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  • 11-6-15 Presentation

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    Sam Boland
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    The document discusses a meeting about manipulating cell migration using amphiphiles. It summarizes results from a previous experiment where staining was not very effective and there was some difficulty imaging. A new method using PDMS placed on the dish before seeding cells was introduced, and preliminary images were shown. The next steps discussed were repeating the experiment with better staining, considering computer aided cell counting, and potentially trying a new method.

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    The document discusses a meeting about manipulating cell migration using amphiphiles. It summarizes results from a previous experiment where staining was not very effective and there was some difficulty imaging. A new method using PDMS placed on the dish before seeding cells was introduced, and preliminary images were shown. The next steps discussed were repeating the experiment with better staining, considering computer aided cell counting, and potentially trying a new method.

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    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    32 views6 pages

    11-6-15 Presentation

    Uploaded by

    Sam Boland
    The document discusses a meeting about manipulating cell migration using amphiphiles. It summarizes results from a previous experiment where staining was not very effective and there was some difficulty imaging. A new method using PDMS placed on the dish before seeding cells was introduced, and preliminary images were shown. The next steps discussed were repeating the experiment with better staining, considering computer aided cell counting, and potentially trying a new method.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 6

    Manipulating Cell

    Migration Using
    Amphiphiles
    11/6/15 Meeting

    Last Time
    DAPI

    Phase

    DAPI

    More reliable results- agree with data from paper


    Staining not very effective
    Slight difficulty imaging

    Phase

    New Method: PDMS


    PDMS placed on surface of dish before seeding
    Once confluence forms, PDMS is removed and
    amphiphiles are applied
    Images taken before migration

    Preliminary Images

    Universal Cell Migration Assembly

    Next Week
    Repeating experiment
    Better DAPI staining
    Depending on the results/ logistics, computer aided
    counting
    Try new method?

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