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Hope
Hope
Virus
Acellular
Bacteria
Cellular
Fungi
Cellular
Parasite
Cellular
Diameter
0.02-0.2 m
0.2-2 m
3-10 m
10-15 m
Nucleic Acid
DNA or RNA
Type of
Nucleus
Ribosome
Absent
Prokaryotic
Eukaryotic
Eukaryotic
Absent
70s
80s
80s
Mitochondria
Absent
Absent
Present
Present
Nature of
Outer Surface
Protein Capsid
and Envelope
Flexible
Membrane
Motility
Non-motile
Non-motile
Non-motile or
Motile
Mitosis
Not by Binary
Fission
Dela Cruz, Renee Hope S.
Replication
Budding or
Mitosis
MT 3-A
Prokaryotes
Contains only 1 chromosome
Lacks Nuclear Membrane
Lacks Mitotic Apparatus
Eukaryotes
Contains more than 1 chromosome
Have a Nuclear Membrane
Have a Mitotic Apparatus
Parasite
Unicellular (Trophozoite and Cyst)
Multicellular (Tapeworm and Adult Ascaris)
Motility:
Non Motile or Motile Flagella Whip-like
Cilia Eyelashes
Pseudopod False Feet
SIZE (Bacteria)
Biggest - Bacillus anthracis
Smallest Mycoplasma
SIZE (Viruses)
Biggest - Pox Virus
Smallest Polio Virus
Arrangement
In Clusters Staphylococcus aureus
In Chains Streptococcus pneumoniae
Kidney Shape Neisseria gonorrhoea
In Rods with Square ends Bacillus
In Rods with Round ends Salmonella
Club-shape Corynebacterium
Fusiform Fusobacterium
Comma-shape Vibrio
Relaxed coiled Borrelia
Tightly coiled Treponema
GRAM POSITIVE
GRAM NEGATIVE
PEPTIDOGLYCAN
Thicker (+)
Thinner (-)
TECHOIC ACID
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Present (+)
Absent (-)
LIPOPOLYSACCHARIDE
Absent (+)
Present
PHOSPHOLIPID
Absent (+)
Present (-)
LIPOPROTEIN
Absent (+)
Present (-)
IMPORTANT PROPERTIES OF CELL WALL
Gram Negative contains ENDOTOXINS (Lipopolysaccharide)
Porin
Pores
Facilitates the transfer of different substances (including drugs) Hydrophobic molecule
Acid Fast Cell Wall
Inability to be gram stained
Mycobacterium and Nocardia
Weak gram positive and refuse to be decolorized by acid-alcohol Stained by CarbolFuchsin
Cell Wall has Mycolic Acid (Increased in Lipids)
Present in 60% of Acid Fast Cell wall
Polysaccharides and Protein are acting as ANTIGEN
Periplasmic Space
The space in between of the cell wall and cell membrane
Peptidoglycan
Compose of peptide and sugar
Shape and support
Interwoven network within the cell wall
NAM N-acetylmuramic Acid
NAG N-acetylglucosamine Acid
Penicillin, Vancomycin, Cephalosporin
inhibits Cell Wall Synthesis
Used in Gram Positive Bacteria
Lysozyme
Present in saliva, tears, and mucus
4
Ribosome
Site of Protein Synthesis
Granules
To store nutrients
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Bacteriocins
Product of plasmid
Lethal to other bacteria
E. coli produce colicins
P. aeruginosa - produce pyocine
Transposons
A piece of DNA is move from one place to another
JUMPING GENES
IMPORTANCE OF CAPSULE:
Virulence Pathogenic
Specific Identification
Quelling Reaction Swelling Phenimenon
Adherence to the Mucosal Tissue
Streptococcus pneumoniae vaccination
Flagella
- Whip-like structure
Propels towards the nutrients
Bacteria causing UTI:
E.coli and Proteus Urethra to Gall Bladder
Chemotaxis movement of bacteria towards to possible nutrient parts: Filament, Hook, and
Basal Body
LOCATION OF FLAGELLA:
Lophotrichous one side
Atrichous none
Amphitrichous both side
Peritrichous - around the cell
Monotrichous one strand
GENERAL RULE:
All cocci are non-motile
All bacilli are motile/ non-motile
Spirochetes- Axial Filament - Flagellum-like structure
Fimbrae - Aids in Attachment
Pili/Pili Conjuncion/ Pili Conjungation
Shorter than a flagella
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Non-motile
Contain Protein pilin
Commonly found in Gram Negative microorganism
Glycocalyx ( Slime layer)
Inhibits phagocytosis
P. aeruginosa
Bacterial Spores
Not for reproduction
Moist and Nutrients Rods of Gram positive
Two Spore-forming Bacteria
Bacillus b. Clostriduim
RULES IN STAINING:
All cocci are gram positive except Neisseria, Moraxella, and Veillonella. All bacilli are
gram negative except Mycobacterium, Corynebcterium, Bacillus, Clostridium,
Erisipelothrix, Listeria, Lactobacillus All spirochetes are gram negative
Fungi or yeast are gram positive
Hans Gram Introduce the Gram Stain
Gram Staining
Crystal Violet Primary Stain Gram Positive Purple
Grams Iodine Mordant Gram Negative Pink or Red
Acetone Alcohol Decolorizer
Safrain Counter Stain
Temperature
Mesophiles Near the Body Temperature ( 20C - 37 C) Psychrophiles Cold-loving
(10C - 20C)
Thermophiles - Heat-loving (50C - 60C)
pH
Acidophiles - Acid (2.5)
Alkalinophiles Base (8.5 )
Halophiles - grows best in salty environment
Most Pathogens - 7.0-7.5 (humans)
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Chemical Requirements
Carbon 50% of dry weight
Structural backbone of all living organism
Essential for Cellular Constituents
Nitrogen - 14 % of dry weight ; Protein Synthesis
Energy - Cellular Function; ATP
Metal and Ions - Sodium, Potassium, Calcium, Chloride
Phosphate, Phospholipids, Sulfur 4 % of dry weight
Fermentation
Breakdown of sugar to pyruvic to lactic acid
Enzymes: Beta-galactosidase Essential for breaking/cleaving of glycosyl Catalase to be
Lactose (glut-1)
: Beta-galactosidase permease Transportation of lactose to the cell wall
Three Major Biochemical Pathways
Embden-Meyerhof glycolytic
PPP Pentose Phosphate Pathway
Entner-Duodoroff Pathway
Anaerobic Pathway (Fermentation)
- Pyruvic and Lactic Acid
Alcoholic Fermentation Ethanol
Homolactic Fermentation Lactic Acid (Streptococcus and some Lactobacillus)
Heterolactic Fermentation Lactic, Formic, Alcohol (some Lactobacillus)
Propionic Acid Fermentation Propionic Acid (Propionicbacterium)
Mixed Acid Fermentation Formic Acid, Lactic Acid, Succine ( E. coli, Salmonella, Shigella)
Bustanediol Fermentation Acetoin (MRVP); Klebsiella, Enterobacter, Seriatia
Butyric Acid Fermentation Butyric Acid and Acetic Acid (Clostridium)
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