EXERCISE 4

Introduction to Proteomics

Submitted by:
Shaldan Chlokie Pendon
Allona Reasol
Alodia Angeline Villa
Rhovil Marie Yecyec

Bio 17L YB

Xavier University Ateneo de Cagayan

September 26, 2015

Table 1. Labels and concentrations of standards

LABEL

STANDARD (mg/ml)

Blank
1
2
3
4
5
6
7
A
B

1 X PBS
0.125
0.250
0.500
0.750
1.000
1.500
2.000
Sample A
Sample B

ABSORBANC
E
0.566 A
0.659 A
0.817 A
1.265 A
1.275 A
1.247 A
2.039 A
2.242 A
1.463 A
1.729 A

CONCENTRATION
(mg/ml)
6.718
7.582
9.541
14.72
14.78
14.47
23.70
26.05
16.67
20.10

Table 2. Unknown protein concentrations

SAMPLE
Sample A
Sample B

ESTIMATED PROTEIN CONCENTRATION (mg/ml)

0.750
1.500

Table 3. Spectrophotometric data for unknown samples

SAMPLE

A595

Sample A
Sample B

1.436 A
1.729 A

ESTIMATED PROTEIN
CONCENTRATION (mg / ml)
0.750
1.500

Table 4. Spectrophotometric data for unknown samples

SAMPLE

ESTIMATED PROTEIN
CONCENTRATION (mg/ml)

Sample A
Sample B

0.750
1.500

QUANTIFIED
SPECTROPHOTOMETER
PROTEIN CONCENTRATION
(mg/ml)
16.67
20.10

Table 5. Standard Curve Absorbance Values

SAMPLE
Std. #1
Std. #2
Std. #3
Std. #4
Std. #5
Std. #6
Std. #7

A595
0.659 A
0.817 A
1.265 A
1.275 A
1.247 A
2.039 A
2.242 A

CONCENTRATION (mg/ml)
0.125
0.250
0.500
0.750
1.000
1.500
2.000

Table 6. Final Concentration of Unknown Samples

SAMPLE

A595

Sample A
Sample B

1.436 A
1.729 A

CONCENTRATION READ
FROM STANDARD
CURVE
(mg / ml)
0.750
1.500

DILUTION
FACTOR

FINAL
CONCENTRATION
(mg/ml)

Table 7. Comparing measured protein concentrations to the values found on food labels
SAMPLE
Sample A
Sample B

BRADFORD ASSAY (mg/ml)

FOOD LABEL (mg/ml)