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CHAPTER III

EXPERIMENT METHOD

3.1 Date and Place


1. Planaria cutting
Day/date
: Wednesday, 30 April 2014
Place
: Zoology laboratory of FKIP Biology Jember University
2. Observe of planaria regeneration
Day/date
: every day in one week, from 31 April 07 May 2014
Place
: Nur Kholifatun Nisa boarding house
3.2 Tools and Materials
1. Razor blade
2. Lup
3. 9 of plastic glasses
4. 9 of elastic bracelet
5. 9 of plastic as covering the glass
6. pin
7. Water medium as the origin planaria habit
8. 6 of Planaria
3.3 Procedure

Prepare the tools and material

Prepare 9 of plastic glass that has filled by water medium as the


origin of planaria habit
Give label (head and body in 3 plastic glasses, right body in 3 plastic
glasses, left body and tail in 3 plastic glasses)

Take 3 of planaria,cut into 3 part. They are head, body and tail.
Take 3 planaria, cut vertically into 2 part. They are left and right
side

Put in plastic glass that has prepared

Cover the plastic glass with plastic cover and elastic bracelet

Make air circulation by stab the plastic cover with pin

Observe the development of regeneration every day in one week,


write all the observation result

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