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Ethanol Precipitation

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Lthano| prec|p|tat|on

1 1/10 volume of 3M naCAC and 3 volume eLhanol were added Lo Lhe unA
2 1he mlxLure was lncubaLed on lce for 13 mlnuLes and laLer cenLrlfuged for 13
mlnuLes aL full speed Lo pelleL unA
3 1 ml of 70 eLhanol was added Lo wash followed by spln for 13 mlnuLes
4 1he unA pelleL was alrdrled before suspenslon ln 10 l 1L buffer

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