Exercise 8: Chemical and Physical Processes of Digestion: Activity 4: Assessing Lipase Digestion of Fat Lab Report

Pre-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.
1. Which of the following is/are true of bile?
You correctly answered: b. It works by a physical process.
2. The substrate used in this simulation is
You correctly answered: c. vegetable oil.
3. When fatty acids are liberated by lipase, the pH
You correctly answered: b. decreases.
4. One of the products of the chemical digestion of lipids is
You correctly answered: d. fatty acids.

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Experiment Results
Predict Question:
Predict Question: Which tube do you think will have the highest lipase activity?
Your answer : a. tube 1
Stop & Think Questions:
What does the pH measure?
You correctly answered: d. both lipase activity and fatty acid release
Why is it difficult to detect whether lipase is active in tube 5?
You correctly answered: d. The pH is already very low, so a decrease in pH might be difficult to detect.
Experiment Data:
Tube No.
1
2
3
4
5
6

Reagent 1
Lipase
Lipase
Lipase
Water
Lipase
Lipase

Reagent 2
Vegetable oil
Vegetable oil
Water
Vegetable oil
Vegetable oil
Vegetable oil

Reagent 3
Bile salts
Water
Bile salts
Bile salts
Bile salts
Bile salts

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Reagent 4
pH 7.0
pH 7.0
pH 9.0
pH 7.0
pH 2.0
pH 9.0

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Time
60
60
60
60
60
60

Temp.
37
37
37
37
37
37

pH
6.21
6.72
9.00
7.00
2.00
8.97

Post-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.
1. What is the product of lipase hydrolysis?
You correctly answered: d. fatty acids
2. From your results, which pH is ideal for pancreatic lipase digestion?
You correctly answered: b. pH 7.0
3. Which tube confirms that there is no lipase in bile salts or vegetable oil?
You correctly answered: c. tube 4
4. From your results, where (in theory) would pancreatic lipase be active?
You correctly answered: d. mouth and pancreas

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Review Sheet Results

1. Explain why you can't fully test the lipase activity in tube 5.
Your answer:
Ya que de por s el pH es muy bajo, siendo dificil de medir la actividad de la lipasa mediante la disminucin del pH con el
acidmetro.
2. Which tube had the highest lipase activity? How well did the results compare with your prediction? Discuss possible
reasons why it may or may not have matched.
Your answer:
El tubo 1 tuvo una mayor actividad de la lipasa. Los resultados muestran lo acertada de la prediccin. La actividad de la
lipasa que es liberada en el intestino delgado es ptima a un pH neutro, si el pH es ms basico, la enzima tendr efectos
menores. Adems, la bilis emulsifica las grasas aumentando la superficie de accin de la enzima. Es por ello que el tubo 1
tubo una mayor actividad enzimtica.
3. Explain why pancreatic lipase would be active in both the mouth and the pancreas.
Your answer:
La lipasa pancreatica seria igual de activo en la boca ya que es la misma enzima que se produce en este medio, teniendo
ambos la misma mnima actividad en comparacin con el intestino delgado. En el intestino, se emulsifica las grasas y
aumenta la superficie de accin enzimtica, siendo en este medio mas eficiente la accin de la lipasa.
4. Describe the process of bile emulsification of lipids and how it improves lipase activity.
Your answer:
En el proceso de emulsificacin, las sales biliares actuan como detergentes, separando los acmulos de lpido y
aumentando la superficie accesible a las enzimas lipasa, mejorando de esta manera la actividad de dicha enzima.

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