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  • Bab Iv Ivo

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    Azhari Gajah
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    The document reports the results and discussion of a study. It presents measurements of absorption curves and calibration curves for sulfadiazine. It shows the concentrations used to create standard solutions and calculates concentrations for calibration curves. Absorption data is presented for sulfadiazine in an artificial stomach fluid medium at pH 1.2, including concentration, absorbance, and calculations for the regression equation to model the relationship between concentration and absorbance.

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    BAB IV IVO

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    The document reports the results and discussion of a study. It presents measurements of absorption curves and calibration curves for sulfadiazine. It shows the concentrations used to create standard solutions and calculates concentrations for calibration curves. Absorption data is presented for sulfadiazine in an artificial stomach fluid medium at pH 1.2, including concentration, absorbance, and calculations for the regression equation to model the relationship between concentration and absorbance.

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    6 views3 pages

    Bab Iv Ivo

    Uploaded by

    Azhari Gajah
    The document reports the results and discussion of a study. It presents measurements of absorption curves and calibration curves for sulfadiazine. It shows the concentrations used to create standard solutions and calculates concentrations for calibration curves. Absorption data is presented for sulfadiazine in an artificial stomach fluid medium at pH 1.2, including concentration, absorbance, and calculations for the regression equation to model the relationship between concentration and absorbance.

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    of 3

    BAB IV

    HASIL DAN PEMBAHASAN

    4.1 HASIL

    Pengukuran Kurva Absorpsi dan Kurva Kalibrasi Sulfadiazin

    LIB I
    250
    C = 1000 x 1000 mcg/ml = 250 ppm

    LIB II
    4
    C = 100 x 250 ppm = 10 ppm

    LIB III

    1. V1. C1 = V2. C2

    4ml. 10 = 10ml. C2

    C2 = 4 ppm

    2. V1. C1 = V2. C2

    5ml. 10 = 10ml. C2

    C2 = 5 ppm

    3. V1. C1 = V2. C2

    6ml. 10 = 10ml. C2

    C2 = 6 ppm

    4. V1. C1 = V2. C2

    7ml. 10 = 10ml. C2

    C2 = 7 ppm

    5. V1. C1 = V2. C2

    8ml. 10 = 10ml. C2
    C2 = 8 ppm

    6. V1. C1 = V2. C2

    9ml. 10 = 10ml. C2

    C2 = 9 ppm

    7. V1. C1 = V2. C2

    10ml. 10 = 10ml. C2

    C2 = 10 ppm

    4.1.1 Data kurva absorpsi Sulfadiazin dalam medium lambung buatan pH 1,2

    No. Konsentrasi Absorbansi X2 Y2 X1Y1

    (X) (Y)

    1. 4 -0,061 16 0,003721 -0,244

    2. 5 -0,050 25 0,0025 -0,25

    3. 6 -0,089 36 0,007921 -0,534

    4. 7 -0,558 49 0,34364 -3,906

    5. 8 -1,561 64 2,436721 -12,488

    6. 9 -0,043 81 0,001849 -0,387

    7. 10 0,025 100 0,000625 0,25

    X=49 Y=2,337
    XY=371 X2=2,764701 Y2=-17,559
    Xrata2=7 Yrata2=0,333
    Persamaan Regresi:

    a
    XY ( X . Y ) / n = 17,559 (49.2,337) / 7 =-0,042
    X ( x ) / n
    2 2
    371 (49) / 7 2

    b=Y-aX=0,333-(-0,042.7)=0,627

    r2
    XY ( X . Y ) / n
    ( X 2 ( X ) / n)( Y ( Y )
    2 2 2
    / n)

    17,559 (49.2,337) / 7
    r2 =-6,649882
    (371 (49) 2 / 7)( 2,764701 (2,337) 2 / 7)

    Persamaan garis regresi:Y=ax+b= Y=0,042x+0,627

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