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Pex 08 03
Pex 08 03
3. In this activity the substrate you will be using to detect protein digestion is
You correctly answered: d. BAPNA.
5. A spectrophometer measures
You correctly answered: c. optical density.
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Experiment Results
Predict Question:
Predict Question: At which pH do you think pepsin will have the highest activity?
Your answer : a. pH 2.0
Experiment Data:
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Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
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Review Sheet Results
1. Describe the effect that boiling had on pepsin and how you could tell that it had that effect.
Your answer:
Al calentarlo no hubo actividad, ya que la enzima se desnaturaliz por las altas temperaturas
2. Was your prediction correct about the optimal pH for pepsin activity? Discuss the physiological correlation behind your
results.
Your answer:
el pH ptimo para la actividad de la enzima es 2, siendo la prediccin acertada. Esto se correlaciona con la fisiologa de la
difestin, en que, al entrar el bolo alimenticio al estmago, se libera HCl, el cual activa el pensingeno a su forma activa, la
pepsina, actuando al pH cido del estmago
3. What do you think would happen if you reduce the incubation time to 30 minutes for tube 5?
Your answer:
La actividad enzimtica seguira siendo nula, ya que el pH no es ptimo para la enzima
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