winCATS Planar Chromatography Manager

Analysis Report
Method D:\TLC SCANNER\My Documents\CAMAG\METODE\Scan
PG.cme
Created by FITOKIMIA Wednesday, December 29, 2010
1:22:22 PM
Last modified by Biologi Farmasi UGM Wednesday, October 08, 2014
11:40:07 AM
SOP document
Validated Design
Description :

Analysis D:\TLC SCANNER\My Documents\CAMAG\DATA\Prak AKTO

2014\G1\20141008-Keji
beling Hidrolisis K2.cna

Created/used by Biologi Farmasi UGM Wednesday, October 08, 2014

11:45:09 AM
Current user Biologi Farmasi UGM

Stationary phase
Executed by Biologi Farmasi UGM Wednesday, October 08, 2014
11:42:54 AM
Plate size (X x Y) 10.0 x 10.0 cm
Material TLC plates silica gel 60 F 254
Manufacturer E. MERCK KGaA
Batch
GLP code
Pre-washing No
Modification No

Definitions - Screening
Executed by Biologi Farmasi UGM Wednesday, October 08, 2014 11:40:40 AM
Batch Product
Substance name Rf Window size Manufacturer number Expiry date number

AutoGenerated1 0.35 0.500

AutoGenerated2 0.01 0.500
AutoGenerated3 0.24 0.500
AutoGenerated4 0.14 0.500

Detection - CAMAG TLC Scanner 3

Information
Application position 10.0 mm
Solvent front position 90.0 mm

Instrument CAMAG TLC Scanner 3 "Scanner3_140718" S/N 140718 (1.14.28)

Executed by Biologi Farmasi UGM Wednesday, October 08, 2014 11:42:54 AM
Number of tracks 1
Position of first track X 91.9 mm
Distance between tracks 0.0 mm
Scan start pos. Y 5.2 mm
Scan end pos. Y 44.0 mm
Slit dimensions 8.00 x 0.40 mm, Macro
Optimize optical system Light
Scanning speed: 20 mm/s
Data resolution: 100 m/step

User : Biologi Farmasi UGM Approved :. . . . . . . . . . . . . . . . . . . SN 1410W025, V1.4.3

Wednesday, October 08, 2014 11:45:11 AM Report ID : 07DE0A08040B2D09 Page 1 of 5
 winCATS Planar Chromatography Manager

Measurement Table
Wavelength 254
Lamp D2 & W
Measurement Type Remission
Measurement Mode Absorption
Optical filter Second order
Detector mode Automatic
PM high voltage 303 V

Detector properties
Y-position for 0 adjust 5.2 mm
Track # for 0 adjust 0
Analog Offset 10%
Sensitivity Automatic (39)

Integration
Properties
Data filtering Savitsky-Golay 7
Baseline correction Lowest Slope
Peak threshold min. slope 5
Peak threshold min. height 10 AU
Peak threshold min. area 50
Peak threshold max. height 990 AU
Track start position 5.9 mm
Track end position 42.9 mm
Display scaling Automatic
All tracks at Wavelength

800

[ AU ]

600

500

400

300

200
100
100 [ mm ]
0 80
-0.10 70
-0.05 60
0.00
0.05 50
0.10 40
0.15
0.20 30
0.25
0.30 20
0.35 10
[ Rf ]
0.45 0

User : Biologi Farmasi UGM Approved :. . . . . . . . . . . . . . . . . . . SN 1410W025, V1.4.3

Wednesday, October 08, 2014 11:45:11 AM Report ID : 07DE0A08040B2D09 Page 2 of 5
 winCATS Planar Chromatography Manager
Track 1, ID:
00 800

00 700

00 600

00 500

00 400

00 300

00 200
AutoGenerated2 @ 254 nm AutoGenerated3
AutoGenerated4 @ 254 nm AutoGenerated1
@ 254 nm @ 254
00 100

0 0
-0.13 -0.03 0.07 0.17 0.28 0.38 0.47 -0.13 -0.03 0.07 0.17 0.28 0.38 0.47
Start Start Max Max Max End End Area
Peak Rf Height Rf Height % Rf Height Area % Assigned substance
Peak deleted by operator
1m -0.04 0.7 0.01 61.0 25.75 0.04 0.0 2721.2 25.53 AutoGenerated2
2 0.07 1.4 0.14 53.1 22.40 0.17 3.6 2339.3 21.95 AutoGenerated4
Peak deleted by operator
3 0.21 1.5 0.24 58.9 24.85 0.28 28.0 2518.2 23.62 AutoGenerated3
Peak deleted by operator
Peak deleted by operator
4m 0.32 1.8 0.35 64.0 27.00 0.41 0.3 3081.2 28.90 AutoGenerated1

Spectrum scan
Executed by Biologi Farmasi UGM Wednesday, October 08, 2014 11:44:56 AM
Mode All detected peaks
Slit dimensions 8.00 x 0.40 mm, Macro
Optimize optical system Resolution
Scanning speed 100 nm/s
Data resolution 1 nm/step
Reference spectrum, pos X 91.9 mm
Reference spectrum, pos Y 5.2 mm

Measurement Table
Lamp D2 & W
Start wavelength 200 nm
End wavelength 500 nm
Measurement type Remission
Measurement Mode Absorption
Optical filter Second order
Detector Mode Automatic

Detector properties
Y-position for 0 adjust 0.0 mm
Track # for 0 adjust 0
Analog Offset 10%
Sensitivity Automatic (27)

User : Biologi Farmasi UGM Approved :. . . . . . . . . . . . . . . . . . . SN 1410W025, V1.4.3

Wednesday, October 08, 2014 11:45:11 AM Report ID : 07DE0A08040B2D09 Page 3 of 5
 winCATS Planar Chromatography Manager

AutoGenerated1 on all Tracks

100.0 100.0
[ AU ] [ AU ]
80.0 80.0
70.0 70.0
60.0 60.0
50.0 50.0
40.0 40.0
30.0 30.0
20.0 20.0
10.0 10.0
0.0 0.0
200.0 250.0 300.0 350.0 400.0 [ nm ] 500.0

T Rf Substance Max. @
1 0.35 Rf AutoGenerated1 321 nm

AutoGenerated2 on all Tracks

100.0 100.0
[ AU ] [ AU ]
80.0 80.0
70.0 70.0
60.0 60.0
50.0 50.0
40.0 40.0
30.0 30.0
20.0 20.0
10.0 10.0
0.0 0.0
200.0 250.0 300.0 350.0 400.0 [ nm ] 500.0

T Rf Substance Max. @
1 0.01 Rf AutoGenerated2 316 nm

AutoGenerated3 on all Tracks

100.0 100.0
[ AU ] [ AU ]
80.0 80.0
70.0 70.0
60.0 60.0
50.0 50.0
40.0 40.0
30.0 30.0
20.0 20.0
10.0 10.0
0.0 0.0
200.0 250.0 300.0 350.0 400.0 [ nm ] 500.0

T Rf Substance Max. @
1 0.24 Rf AutoGenerated3 316 nm

User : Biologi Farmasi UGM Approved :. . . . . . . . . . . . . . . . . . . SN 1410W025, V1.4.3

Wednesday, October 08, 2014 11:45:11 AM Report ID : 07DE0A08040B2D09 Page 4 of 5
 winCATS Planar Chromatography Manager

AutoGenerated4 on all Tracks

100.0 100.0
[ AU ] [ AU ]
80.0 80.0
70.0 70.0
60.0 60.0
50.0 50.0
40.0 40.0
30.0 30.0
20.0 20.0
10.0 10.0
0.0 0.0
200.0 250.0 300.0 350.0 400.0 [ nm ] 500.0

T Rf Substance Max. @
1 0.14 Rf AutoGenerated4 218 nm

Evaluation results

Evaluation Sequence
Track Track type Vial Sample ID
1 Sample 1

Table of substances
Position Tracks
Substance MD mm 1
AutoGenerated1 38.0 m
AutoGenerated2 10.6 m
AutoGenerated3 29.5 A
AutoGenerated4 21.3 A

User : Biologi Farmasi UGM Approved :. . . . . . . . . . . . . . . . . . . SN 1410W025, V1.4.3

Wednesday, October 08, 2014 11:45:11 AM Report ID : 07DE0A08040B2D09 Page 5 of 5