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Water Quailty Lab
Water Quailty Lab
Aim Question
Water quality, known as the physical, biological, chemical and radiological
characteristics of water, its condition can greatly affect the health of living organisms
in it. It can be measure by different methods such as using machine or biological
indicators. Biological indicators, refers to the organisms that are sensitive to the
presence of pollutants in a specific environment. For aquatic environment, an
organism that is commonly used to measure the water quality is Artemia (Brine
Shrimp), it is a typical primitive arthropod that is very tiny and lives in salt-water
lakes. However, we don’t have advance machine to help us measure the relative
water quality of some water sources, so how can we compare and test the relative
water quality for different water source? Are there any differences between tap
water and canal water? Does bottle water really healthy? As a result, during this
experiment, we are going to use the biological indicators---Artemia to measure the
relative water quality in order to find these answers.
We are going to measure the relative water
quality of three sources of water, tap water,
canal water and bottle water. The tap water is
from the school laboratory, we will remove the
chorine before put in the artemia. The canal
water is from the river nearby our school, we will
boil it before use. The bottle water is normal
drinking water. For each water source, we are
going to add salts in order to provide a salinity aquatic environment for Artemia. For
each water source, we will measure both of the growth and hatching rate of
Artemia in it in order to assess the water quality, there will be two days for hatching
rate and two days for growth rate.
B. Hypothesis
I predict that the hatching rate of artemias in bottle water will be the highest,
because there are no bacteria or microorganism and other toxic substances such as
heavy metals, cadmium, mercury, lead present in the bottle water (Drinking water).
Also, no bacteria or microorganism present means that there aren’t other living
things would take in the oxygen in water that decrease the oxygen level for artemia
eggs. Since there are no toxic substances that might poison and affect the hatch of
artemias, no other living organisms to take away the oxygen, I assume most of the
artemias in bottle water will successfully hatched.
I predict that the growth/survive rate of artemias in canal water will be the
highest. Since the canal water that we used were already been boiled, so that the
small bacteria or other tiny planktons will become dead in canal water. Thus, their
bodies can existed as food in canal water for artemia that lives in it. As a result, there
will be more artemias have chances to survive in canal water. In the meantime,
nature artemia live in salty river, so that canal water is very suitable for the growth
of artemias.
I predict the growth/survive rate of artemias in tap water will be similar with
canal, also very high because tap water contain a lot of microorganisms, and also
some of the single cell plant-like organisms. These plant-like organisms can do
photosynthesis inside tap water, thus take in the carbon dioxide and produce oxygen
for artemias, thus the tap water will rich in oxygen that able to let artemia survive
for a longer time. Also, the tap water that we extract from school and house were all
after many purification process, such as dechloridation, filtration, so that there won’t
be many toxic pollutants and bacteria in it.
I predict that the artemias will be least able to survive in bottle water. Since
bottle water is the drinking water for people, so there won’t be any bacteria, organic
nutrient and other tiny planktons lived in it. Thus, bottle water can’t provide food for
artemias. Also, if the bottle water contains many artemias that were hatched, this
also can justify that artemia will be least able to survive in bottle water because the
lack of oxygen level. Eventually, oxygen will be used up by these artemias. Because
we don’t have air pump, so lack of oxygen=death of artemia.
C. Variables
Independent Variable: Water source
In order to manipulate the independent variable, we are going to prepare three
different kinds of water sources, which are tap water, bottle water and canal water.
For tap water, we are going to use the water from the tap inside the laboratory, we
need to first remove the chorine in the tap water by placing the tap water under to
sunlight for approximately 2-3 days in order to let chorine water decomposed to
hydrochloric acid (HCL) and oxygen, because HCL is easy to volatized, so chorine can
be remove. Chorine is necessary to remove because its poisonous and it can possible
damage the health of artemia.
For canal water, since there are a lot of microorganisms live in it and also a lot of
pollutants might existed, so we will boiled the canal water before use as a living
environment for artemia in order to kill most of the microorganisms and deactivated
the pollutants.
For bottle water, we will use the Nong Fu Spring Water. The amount of all the water
sources will be about 10 milliliter, we will measure it by using the measure cylinder.
Besides, artemia need to survive in salt water, it has been found that most artemia
species hatch best in salt concentration of about 30g per liter. Though calculation,
30g salt per liter=0.03g salt per milliliter, we will measure 0.3g of salt by using
electric balance and pout them in each 10ml water source.
Control Variables:
1. The salt concentration in water source
Why to control: Artemia need to live in a salty environment in order to survive. If
there is no salt present in water or the concentration of salt cannot reach the
artemia’s requirement, artemia will not be able to hatched or it will died very soon
after hatched. Salt water is the basic requirements to hatch artemia. Also, different
salt concentration in water might affect the health condition of artemia and result
different hatch and growth rate, so it is important to maintain the salt concentration
to be same in each test tube in every water source.
How to control: We can add the same concentration of salt in each water source.
Since it has been found that most artemia species hatch best in salt concentration of
about 30g per liter. Though calculation, 30g salt per liter=0.03g salt per milliliter, we
will measure 0.3g of salt by using an accurate electric balance and pout them in each
10ml water source.
D. Apparatus
1: 100ml Measure cylinder*1
2: 250ml Beaker*5
3: Test tube*12
4: Electric balance*1
5: Glass rod*1
6: Dropper*5
7: Test tube rack*2
8: Marker*1
9: Tissue paper*1 pack
10: Recording Pan*1
11: Recording sheet*1
12: Magnifier*1
13: 40ml tap water
14: 40ml bottle water
15: 40ml canal water
16: Sea Monkey/Brine Shrimp/Artemia*120
17: PH Paper*12
18: Tweezers*1
19: 5 grams of edible salt
20: 12 pieces of pan paper
E. Safety Precautions
1. Do not drink and taste any liquid inside the lab during the experiment, especially
those with artemia in it.
2. Be careful with the glass-made apparatus during this experiment, put them
horizontally on the desk if didn’t need to use.
3. Do not play with the artemia eggs, carefully place them inside the test tube, do
not spill them all over the ground.
4. Be careful with the test tube that contain artemia eggs in water, do not overturn
the bottles and let the water source and artemia eggs sprinkle to the ground.
5. Do not put the artemia eggs inside month.
6. Do not taste the edible salt that is used to carry out the experiment.
F. Method
1.Prepare all the appliances that will be involved in this experiment, such as the tap
water, bottle water, canal water, beaker, measuring cylinder, dropper, edible salt,
electric balance, test tube, artemia eggs, etc.
2. Using tweezers to put the PH paper in to each water source in order to test the PH
value of tap, canal and bottle water.
3. Using one measuring cylinder to measure 10 ml of tap water and pour it in to one
breaker.
4. Put a piece of pan paper on the electric balance and push the net-weight bottom.
5. Using a spatula to take some edible salt and place on the pan paper.
6. Add or take out the edible salt until the screen on the electric balance display 0.3g.
7. Pour the pan paper that contain 0.3g edible salts into the beaker that contain 10ml
of tap water.
8. Using glass rod to stir the liquid in order to mix the edible salt and tap water
together until the salt is fully dissolved, which there shall be no crystals existing.
9. Clean the glass rod by using the tissue paper after mixing one water source in
order to prevent impurities in one particular water source being brought to another
water source by the glass rod.
10. Pour the salty tap water from the beaker in to one of the test tube, hence using
marker to label the test tube and carefully put the test tube to the big test tube rack.
11. Repeat step 2 to step 8 to do other three trials of tap water.
12. Repeat step 2 to step 9 by replacing the 10ml tap water to 10ml bottle water and
10ml canal water respectively.
13. Carefully count ten artemia eggs by using one magnifier.
14. Using the wet glass rod to stick ten artemia eggs and carefully place them to one
test tube of tap water.
15. Repeat step 13 to 14 with other eleven test tubes of tap water, canal water and
bottle water.
16: Check the side of each test tube to see if there are any artemia eggs sticks on the
test tube, using one dropper to absorb some water inside the test tube and scour the
glass side in order to let the artemia eggs go back the water part in test tube.
17: Arrange all the test tubes with artemia eggs on the test tube rack, put the test
tube with same water source side-by-side, double check the label on each test tube.
18. Wait for two days (48 hours) to see how many artemia eggs successfully hatched
in each test tube and meanwhile record the data on a sheet of paper.
19. After one day, shake each of the test tubes to allow oxygen mix with the water
and also see how many artemia still survive and meanwhile record the data.
20. Repeat step 19 for the next day.
21. After collect all the data, calculate the hatching rate by multiply the number of
hatched eggs in each test tube by 10. Calculate the growth (survive) rate by multiply
the number of survive artemia in each test tube in each day by 10.
22. Draw the finial conclusion according to the calculated result.
Table one:
Hatching rate for 48 hours
(%)
Types of water/tube Tube 1 Tube 2 Tube 3 Tube 4 Average
Tap water (4)*10=40% (5) *10=50% (5) *10=50% (3) *10=30% (4) *10=40%
Canal water (4)*10=40% (2) *10=20% (2) *10=20% (4) *10=40% (3) *10=30%
Bottle water (2)*10=20% (3) *10=30% (2) *10=20% (1) *10=10% (2) *10=20%
Table two:
Survival rate for 24 hours
(%)
Types of water/tube Tube 1 Tube 2 Tube 3 Tube 4 Average
Tap water (2) *10=20% (2) *10=20% (1) *10=0% (0) *10=0% (1) *10=10%
Canal water (3) *10=30% (1) *10=10% (2) *10=20% (3) *10=30% (2) *10=20%
Bottle water (1) *10=10% (1) *10=10% (0) *10=0% (0) *10=0% (1) *10=10%
Table three:
Survival rate for 48 hours
(%)
Types of water/tube Tube 1 Tube 2 Tube 3 Tube 4 Average
Tap water (2) *10=20% (0) *10=0% (0) *10=0% (0) *10=0% (0) *10=0%
Canal water (1) *10=10% (1) *10=10% (0) *10=0% (2) *10=20% (1) *10=10%
Bottle water (0) *10=0% (0) *10=0% (0) *10=0% (0) *10=0% (0) *10=0%
Column Graph
Graph One:
60
Tap water
50
Canal water
40
Bottle water
(%)
30
20
10
0
tube 1 tube 2 tube 3 tube 4 average
different water source (no units)
*Blue column= Tap water, Red column=Canal water, Green column= Bottle water
There are four tubes for each water source.
Calculation for hatching rate: 4 artemia in 10 artemia eggs are hatched, since
10*10=100%, so 4*10=40%, so there are 40% of artemia were hatched in tube 1 in tap
water. (This is only in simple terms, the real calculation process will be discussed in the
data analyzes part)
Graph Two:
40
Average hatching rate (%)
30
20
10
0
tap water canal water bottle water
Different types of water source ( no units)
Graph Three:
The survival rate for Artemia in 24 hours
35
survival rate for artemia in 24
30
25
20
hours(%)
Tap water
15
Canal water
10
5 Bottle water
0
tube 1 tube 2 tube 3 tube 4 average
Different water source (no units)
Graph Four:
20
15
10
0
tap water canal water bottle water
Different types of water source ( no units)
Graph Five:
25
20
48 hours(%)
Tap water
15
Canal water
10
Bottle water
5
0
tube 1 tube 2 tube 3 tube 4 average
Different types of water source ( no units )
0
tap water canal water bottle water
Different types of water source ( no units)
H. Data Analyze:
According to the column graph one and graph two, we can easily find out that tap
water has the highest hatching rate for artemia, which there were 40% of artemia
hatched in tap water in average. From opposite, bottle water has the lowest
hatching rate for artemia, which there were only 20% of artemia hatched in bottle
water. Canal water has a medium rate that is high to 30%.
In simple terms, the hatching rate is calculated by using the original number of
hatched artemia multiply by 10.
For deeper calculation process, for example, there are five artemia in ten eggs were
hatched in tap water.
Because the fraction was written as 5/10, which means 5÷10=0.5.
Next step, we need to convert number into percentage, so we need to use
0.5*100%=50%.
As a result, there are 50% of artemia were hatched.
For Graph Two, the average hatching rate for each specific water source is calculate
by add the data that collect in four test tube together and divide by four.
For example for the average hatching rate of canal water, first we add the data in
four test tube, the sum of the hatched artemia in four test tubes is given by:
4+2+2+3=11 artemia were hatched in four test tube.
Than, we can use 11÷4=2.75 artemia were hatched in each test tube
Finally, we can use round the result to the nearest whole number, which 2.75 will
become 3.
Using the percentage calculation process before, 3/10. 3÷10=0.3, 0.3*100%=30%,
we can get that there are 30% of artemia in canal water were hatched in average.
According to the column graph three and graph four, we can conclude that
artemia has the highest survive rate in canal water, which there are 20% of artemia
successfully survive. 3+1+2+3=9(artemia in total), 9÷4=2.25, 2.25≈2. (Average No.
Of artemia survive in each test tube.) 2/10, 2÷10=0.2, 0.2*100%=20%. From
opposite, bottle water and tap water contain artemia that has lowest survival rate,
which both are only 10%.
According to the column graph five and graph six, we can find out that all the
artemia that lived in bottle water were all fail to survive after 48 hours, and there
are only 20% of artemia survived in one test tube of tap water. But for canal water,
most of the artemia were survived in each test tube of canal water expect test tube 3,
and the average survival rate of artemia in canal water were up to 10%.
*The number of artemia that had hatched or survived were all on the low side,
which there were no one single test tube for any water source contain more than 5
hatched/survived artemia. I think there might be three reasons for this phenomenon.
First, this might due to the water quality, which the water quality is unable to
provide a comfortable environment for many artemia to survive. Second, this might
due to the egg quality. Sometimes the egg is dead before hatched, the egg might be
damage or cannot develop into a artemia, in this way, the egg will never hatched no
matter how suitable and how good the water quality is. Also, these eggs were easy
to stick on the side of glass rather than in the water. This could cause anomalous
result that eggs not in the water could never have hatched. This almost happened in
each test tube, especially in canal water. Thus, if I successfully clean all the artemia
eggs that stuck on the glass side back to canal water, the hatching rate and survival
rate of artemia will increase.
*According to the dose response curve, even there might be some heavy metals in
the tap water that are not been filtered, and some in the canal water due to the
water pollution, because the dose of these heavy metals were not high enough to
affect the indicators, so there aren’t be any observable effect on indicators.
I. Conclusion:
During this scientific investigation, the result from my experiment is not in one
hundred percentage same as my hypothesis. The summery of my result was:
Artemia eggs had the highest hatching rate in tap water, highest survival/growth
rate in canal water, lowest hatching rate and lowest survival/growth rate in bottle
water.
For tap water, it is the water that produced by the purification and disinfection
process in water treatment plant in order to reach relevant standards for people’ life
and usage. It is mainly from rivers, lakes, groundwater or surface water. It will go
though precipitation, disinfection, filtration and other technological processes
before reach the pumping station and finally transport to each user. Generally, it
does not have many harmful pollutants such as heavy metals, cadmium, lead, or
mercury since it need to be safe for people to use. However, there are one substance
in the tap water that are poisonous and could affect the health of both artemia and
people---chorine. Chorine is added during the process of tap water purification, the
purpose is to resist and kill bacteria in the water. But chorine is poisonous and it
could affect the health of living things, so we remove the chorine in the tap water by
placing the tap water under to sunlight for approximately 2-3 days. Tap water
without chlorine is a good place for the hatching of artemia since there weren’t many
poisonous substances and pollutants present in it, there were only some single
celled plant-like organisms left. These plant-like organisms have tenacious vitality,
and they could do photosynthesis that take in carbon dioxide and produce oxygen,
thus the artemia eggs can hatch very effectively in this oxygen-rich environment.
This can prove the first result of my experiment is reasonable.
For canal water, artemia is most adept to survival in it. According to the Wikipedia
for brine shrimp (Artemia), artemia usually live in salty river in nature, so salty canal
water is the real living condition for artemia, which is very suitable for them to
survive. Also, we boiled the canal water before the experiment, so that most of the
bacteria and living organisms that will absorbed oxygen were gone, there were no
other living things would “rob” the oxygen level from artemia. Besides, the dead
bodies of these microorganisms can act as the food for artemia to eat, thus more
artemia would able to survive. Also as same as the tap water, there will be some
plant-like organisms that own tenacious vitality can carry out photosynthesis to
produce more oxygen for artemia. Overall, artemia have the highest survival rate in
canal water due to the high level of oxygen and abundant food supply.
For bottle water, it has the lowest hatching rate and lowest survival rate. This is
different from my hypothesis. Since there are no any microorganisms present in
bottle water, so that the artemia living in bottle water didn’t have any food supply,
which this is one of the reason that it has such low survival rate. Besides, we didn’t
shake the test tube for bottle water during the two days hatching, we only shake
after the artemia was hatched, so there were no oxygen goes into the water. Also,
there are no plant-like organisms that are able to do the photosynthesis and provide
extra oxygen for artemia, so the oxygen level in bottle water will only eventually ran
off instead of increase, that is not good for both hatching and survival.
Things that we done well: However, apart of these mistakes and errors during the
process of experiment, our group also did some parts very valid.
First, we remember to shake the test tube during measuring the survival rate for
each water source during every lunch period and sometimes during break in order
the let the oxygen go into the water. This is a very important step that could allow
more oxygen go into the water for artemia to use.
Second, we control the temperature of the water source by placing the test tube
inside the shade of the laboratory and avoid directly contract with the sunshine. This
make the temperature keep constant during hatching, which will not affect the
normal hatching rate of artemia.
Third, we also maintain the PH value for each water source, each water source has a
PH value around 6 and 7, which is suitable for the hatching and survival of artemia.
Fourth, our method for this experiment allows us to collect sufficient collection of
data. We use four test tubes to do four trails for each water source during this
experiment and we calculate the average hatching and growth rate for each water
source by adding the sum of hatched/survived artemia in each test tube for each
water source together and divide the amount of test tube. This makes the result and
our data more reliable and referable than only do one single trail for each water
source. Also, we choose to use large sample sizes of artemia in each test tube. We
had 10 artemia eggs in each test tube for each water source, which is a simple size
that is large enough to give trustable result.
-END-
Water Quality Lab
Name: Apple Chen
Class: MYP 9(4)
Number: 20129401