Describe a technique to show translocation

Bark ringing method

- Remove a ring of bark from stem
- Leave for a minimum of one day
- Analyse content of tissue above ring
Labelled carbon dioxide and potted plant
- Supply plant with radioactive CO2
- Allow to photosynthesises
- Use autoradiography to identify substances
Aphids
- Allow aphids to feed on stem
- Cut off proboscis and collect sap
- Use chromatography to identify substances

Describe the transport of organic compounds in vascular plants

- Phloem transports sucrose
- From sources to sinks
- Sources are the photosynthetic tissues/leaves
- Sinks are fruits, seeds, roots
- Through sieve tubes with sieve plates
- Loading of sucrose into phloem by active transport
- High solute concentration causes water to enter by osmosis
- High hydrostatic pressure causes flow from source to sink
- Companion cells help with loading
- Translocation/Mass flow