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The Simple Anti-oxidant Compound in Anti-cancer... Similarity Index

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Proceedings of

International Conference on Basic Science 30

(ICBS) “The Role of Basic Science in Health, Environment, and Advance Technology”

Faculty of Science, University of Brawijaya, 30

Malang, Indonesia Widyaloka Convention Hall, February, 17 – 18th 2011 Keynote Speakers 1. Assoc. Prof. Spas D. Kolev
– University of Melbourne (Australia) 2. Dr. Edgar Paski – British Columbia Institute of Technology (Canada) 3. Prof. Dr.

Lukman Hakim – Indonesian Institute of Science (Indonesia) 4. Prof Dr. Ir. Chandrawati Cahyani, MS – University of

Brawijaya (Indonesia) Board of Advisors Prof. Dr. Ir. Yogi Sugito (UB – Indonesia) Prof. Dr. Marjono, MPhil (UB –
Indonesia) Prof. Dr. Ir. Chandrawati Cahyani, MS (UB – Indonesia) Prof. Dr. drh. Aulanniam, DES (UB – Indonesia) Prof.

Dr. Loekito Soehono, M.Agr (UB – Indonesia) Prof. Petr Solich (Charles University – Rep. of Czech) Assoc. Prof. Stuart R.
Batten (Monash University – Australia) Dr. Masayuki Sano (AIST – Japan) Dr. Edgar Paski (British Columbia Institute of

Technology – Canada) Board of Editors Dr. Hermin Sulistyarti (UB – Indonesia) – Editor in chief Prof. Mick O’Neill

(Advisory and Training Service Pty, Ltd – Australia) Prof. Petr Solich (Charles University – Rep. of Czech) Dr. Edgar Paski

(British Columbia Institute of Technology – Canada) Dr. Francois Malherbe (Swinburne University of Technology -
Australia) Dr. Lilibeth dlC. Coo (University of the Philiphine - Philiphine) Dr. Verasak (King Mongkut's University of

Technology - Thailand) Zubaidah Ningsih, M. Phil (UB – Indonesia) Irfan Mustafa, MSi (UB – Indonesia) Yuniar Ponco

Prananto, MSc (UB – Indonesia) Design and Layout Hary Agustiawan Armeida Dwi Ridhowati Madjid Publisher: Galaxy

Science Publisher, Malang, Indonesia ISBN: 978-602-97628-5-3 PREFACE

Proceeding of the International Conference on Basic Science 4

2011

is a book containing several papers that have been presented in the 1

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seminar, either oral or in poster. The objective of this proceeding is to collect and to share any useful information that

had been gathered recently in area of Mathematics, Biology, Chemistry and Physics, which is generally known as the

basic science. Hence, through this book the readers are expected to gain new knowledge or information, especially in

health, environment and advance technology. In general, the papers are presented in order of health (part A),
environment (part B) and advance technology (part C) in which in each part the papers are divided into two sections, the

oral and poster papers. Many papers in this proceeding discuss the exploration and the development of natural

resources as the medicine based compound source. Furthermore, the modi cation of analyzing method and modeling

in health and environmental science, whether it is based on nuclear technology or advance biotechnology, has also been

compiled in this proceeding. In addition, several interesting topics which present the latest information in environmental
eld add the usefulness of information gathered in this proceeding. Additional information of the rst author is also

given at the end of this book. Hence further contact to the author is possible via the email address available at the

a liation section. Should the readers have further questions, comments or need more information, please directly
contact the authors of the papers. The editorial board

has made some editing correction needed in some cases. Most of the editing correction are 1
conducted and concentrated in the organization of the paper based on the guideline and the language,

i.e. some gures or tables were corrected, or maybe printed in

grayscale, and placed in the last part of the article (appendix).

In addition, the language is the most time-consuming work; hence 1

we apologize for the late publishing of this book and for any inconvenience as a result of the delay. 1

Lastly, on behalf of the committee, we would like to say thank you to all the participants for their kindness to be part of

this conference. We also would like to acknowledge each partnerships and sponsorships that involved during this event.

We believe that this proceeding still has some weaknesses; therefore any constructive comments are welcome. Once

again, thank you to all, have a nice academic life and see you in the next ICBS. Malang, May 2011 i Message from

President of ICBS 2011 Praise to Allah SWT, as only of his love and blessing, our 1stth International conference on Basic

Science (ICBS 2011) was successfully held on February 17-18th, 2011 in Widyaloka Building University of Brawijaya
Malang Indonesia, as one of celebration for the 48thAnniversary University of Brawijaya and the International Year of

Chemistry 2011. The conference was projected to facilitate the discussion of the truth and beauty of science and its

application to impact positive improvement in health, environment and advance technology for prosperity to the local

society and worldwide. We would like to thank and give highest appreciation to Rector University of Brawijaya and Dean

Faculty of Science for their fully support and to Our honourable and generous keynote and invited speakers: Dr. Edgar

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Paski from BCIT & Analytical Innovation Canada; Assoc. Prof. Spas Dimitrov Kolev, from The University of Melbourne,

Australia, Prof. Lukman Hakim, Head of Indonesian Institute of Science, Prof. Chandrawati Cahyani from Chemistry

Department University of Brawijaya Malang Indonesia, Prof. Brenny

van Groesen from University of Twente, Netherlands and LabMath-Indonesia, Bandung 32

Indonesia;

Prof. Motoki Kubo and Prof Mamoru Wakayama from Department of Biotechnology, Ritsumeikan University, Japan; Dr.

Francois Malherbe from Swinburne University of Technology, Australia. Another great appreciation goes to the board of

editors: Assoc Prof. Lilibeth Coo from University of Philippines Manila, Prof. Mick O’Neill, the Director Statistical

Advisory & Training Services Australia, and Prof. Petr Solich from Charles University Republic of Czech. Many thanks go

as well to all national & international participants for their contributions to the conference program and to this

proceeding. It is our pleasant duty to also acknowledge our sponsors: PT Ar ndo Bersinar, CV Makmur Sejati,

Indonesian Forestry Department East Java Division, the Local Government of Malang City, Indonesian Chemistry

Society, PT. Otsuka Lawang, PT. Amerta Indah Otsuka - Pasuruan, Kristalindo, Trans Zahwaa Travel, Elfara FM and Tugu

Hotel as your strong support and active participation have made the 1st ICBS becomes a reality. The material in this

proceeding is divided into three parts: (1) Health, (2) Environment, and (3) Advance Technology. We hope that the
papers contained in this proceeding will prove helpful toward improving international collaboration research to solve the

problems related to heath, environment, and advance technology. As this conference is held annually, we are looking

forward to the 2nd International Conference on Basic Science that will be held on February, 2012 at the same location.

We hope that it will be a more interesting and enjoying conference. President Dr. Hermin Sulistyarti ii Message from

Dean of Faculty of Science – UB On behalf of the faculty of Science we are very happy to complete the 1stth

International conference on Basic Science (ICBS 2011) by this proceeding. Our appreciation to

Prof. Dr. Ir. Yogi Sugito, rector of the University of Brawijaya 38

for his support and all of the keynote and invited speakers who made this conference succeeds. This proceeding
contains as the following. Part A: Health, Part B: Environment, and Part C: Advanced Technology. Oral papers are

followed by posters at each part. Finally, we would like to thank all of the steering and organizing committee for their
efforts in succeeding this conference, especially to Dr. Hermin Sulistyarti as the chair of the organizing committee. Last
but not least for all of the participants, we are thankful for your cooperation, contribution and very valuable support for

this event. Thank you, Prof. Dr. Marjono, MPhil. iii Table of Content Preface Message from President of ICBS 2011
Message from the Dean of Science Faculty of UB Table of Content i ii iii iv Part A. Health Section A.1. Oral Presentation

A.2. Poster Presentation Part B. Environment Section B.1. Oral Presentation B.2. Poster Presentation Part C. Advance
Technology Section C.1. Oral Presentation C.2. Poster Presentation 1 – 106 107 – 181 182 – 299 300 – 366 367 – 490

490 – 617 Erratum 618 – 619 Bibliography of First Author Sponsorships xvi xxiii iv Part A. Health Section A.1. Oral
Presentation

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CD8+CD122+ Regulatory T Cells is a New Therapy for In ammatory Bowel Disease 23

Agustina T Endharti, Nobuaki Misawa, Masafumi Ito, Haruhiko Suzuki ...................................... 1 – 106 1

Cytotoxic, antioxidant and antibacterial activity of methanol extract of Xylocarpus moluccensis fruit 4
husk

Asep Awaludin Prihanto ............................................................................................................... 2

Research-based on Herbs Exploration and Use of Animal Models: Nature Materials towards 15
Supporting Evidence Based Medicine
Aulanni’am....................................................................................................................................

The Effect of Red Guajava (Psidium guajava) Fruit Extract on Tyrosinase (EC 1.14.18.1) Activity 14

by Spectrophotometry Ayik Rosita Puspaningtyas

.......................................................................................................... 10 Study of Toxicity Effect of

Formalin on Antioxidant Enzymatic Activities and Oxidative Damages of Rats Hepar Tissue Chanif Mahdi, Aulanni’am
.......................................................................................................... 15 Monoclonal Antibodies to Bovine Zonna Pellucida 3

(Mab-bZP3) for Woman Immunocontraceptive Vaccine Dedy Kurniawan, Sutiman B Sumitro,

Aulanni’am...................................................................... 19 Monitoring and Capability Analysis of Hemoglobin Level in

Pregnant Women Dewi Anggraini............................................................................................................................ 22 Anti-

In ammatory of Methanolic Fraction from 96% Ethanolic Extract of Honey Bee Propolis on White Male Rats Wistar

Strain Dwi Ningsih, Agustina Wulandari, Gunawan Pamudji Widodo ................................................. 27 Evaluation on the
Phytoestrogenic Effect of Legumes Extract in Binding Competition on Estrogen Receptor Edi Priyo Utomo
........................................................................................................................ 33 Preparation of Constructs Gene sY86 as the

Basis of Antibody Formation for Determination Method of Male Infertility Evi Hanizar, Miswar
................................................................................................................... 34

Function of hINSR Mutant Againts Tyrosine Kinase Precede Abnormally on Onset Diabetes 12
Mellitus: In Silico Study Fatchiyah,

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NMA. Putri , D. W. Soeamadji .................................................................................. 37 The Analysis of Ca2+ Intensity Pro le

of M-II Oocyte Natural Activated by Crude Sperm Extract Gatot Ciptadi

............................................................................................................................. 38 The Effect of Prostaglandin F2α Addition

on Sperm Quality in Goat Semen Diluted with Various Solutions Herawati

....................................................................................................................................... 42 v Correlation Between AgNOR, MIB-

1 and Radiotherapy Response in Lung Cancer Iin Kurnia, Heriawati, Juniarti, Ruth ES, Zubaidah Alatas, Elisna Syahruddin,
Made Widhyasmara….………………………………..………………………………………………………………….……….......

Tyrosinase Activity of Piper betle and Piper crocatum Essential Oil 2

Irmanida Batubara, Min Rahminiwati,Latifah K. Darusman, Tohru Mitsunaga 2

...................... Immune system impairment in CD122-de cient mice is caused by abnormality of activated T cells
Muhaimin Rifa'i………………………………………………..…………………………………………………………………. The Simple Anti-oxidant Compound

in Anti-cancer Potential Fraction of Kesum Leaves Extract Muhamad Agus Wibowo, Lucy Ariani, Aulanni’am, M. Aris
Widodo, Basuki B. Purnomo ....... Cytotoxicity,

Antioxidant and Antimicrobial Activity of Methanol Extract of Bark (Xylocarpus 4

Granatum) Muhamad Firdaus ....................................................................................................................... Developing

Malaria Vaccine Candidate with Gamma Rays: Alteration of Heat Shock Protein Pro les Post Irradiation Mukh Syaifudin,
Devita Tetriana, Siti Nurhayati, Darlina, Tur Rahardjo, Irawan Sugoro ........... Polyphenol Extract of Brown Seaweed

(Sargassum duplicatum Bory): Phytopharmaco Exploration for In ammatory Bowel Disease Theraphy Nur Lailatul
Rahmah, Aulanni’am, Anna Roosdiana ..................................................................

Agrobacterium Mediated Transformation of Tomato and Production of Transgenic Plants 25

Containing Gene

Increased Sucrose Transport SoSUT1 Parawita Dewanti, Bernet Agung Saputra, Tatik Wardiyati, Ari n Nur Sugiarto,
Bambang Sugiharto.................................................................................................................................... Cytotoxicity,

Antioxidant and Antibacterial Activities of Methanol Extract of Xylocarpus Moluccensis Lam. 4

Fruit

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Peel Rahmi Nurdiani ......................................................................................................................... Study of Antibacterial and

Cytotoxic Activity of Porphyra Sp Extract Raja B.D. Sormin, Sukoso, Happy Nursyam, Siti Rasminah Ch. Sy
................................................ Superoxide Dismutase (SOD) Activity and Histological Pancreas Tissue of Type 1 Diabetes

Mellitus Rat Treated with Temu Giring Extract (Curcuma heyneana) Rosalina A.L., Aulanni’am, Chanif Mahdi
................................................................................... Characterization of Allergen Proteins and Alergenicity of Porang

tubers (Amorphophallus muelleri Blume.) by using IgE-Immunoblotting Sofy Permana, Sri Widyarti, Luccy Mutia Lingga
Dewi, Indrian Rizka Amalia ............................

Growth Differentiation Factor-9 Gene Expression of Mice Oocytes in Vitro 28

Sri Rahayu , Manami Nishio , Yumi Hoshino , Eimei Sato .......................................................... In Vitro Anti-Malarial Activity
of Alkaloids from Erythrina Variegata against Plasmodium Falciparum Tati Herlina , Unang Supratman , Syafruddin ,

Hideo Hayashi .................................................. 45 50 54 59 62 65 70 75 79 83 87 92 93 97 vi Recognition Antigen and

Antibody Speci c Protein 24 and Protein 40 Borna Virus in Serum Patients Psychiatric Disorders Tri Maharani ….

……………………………………………………………………………………………………………………… Lactobacillus Plantarum S1.30 Isolated from Dadih

has Antagonistic Properties and Harbours Gene Encoding the Production of Bacteriocin

Yoga Dwi Jatmiko, Miguel De Barros Lopes, Mary Barton ......................................................... 101 31
102 A.

2. Poster Presentation 107 - 181 Aloe Gel Herbal Therapy on Diabetic Ulcer Healing Adeodatus Yuda Handaya ,
Djanggan Sargowo, M. Aris Widodo, Diana Lyrawati, Askandar Tjokroprawiro

............................................................................................................. 107 The Effect of Teaching of Vitamin C using

Problem Based Learning and Laboratory Works on Students Achievement Afnidar, Sri Hamda, Erdawati

...................................................................................................... 112 Isomerization and Toxicity Test of Methyl Linolenic

Ester of α-Linolenic Acid Isolated from Ocimum Basilicum Seeds A. Ghanaim Fasya , Rurini Retnowati , Moh. Farid

Rahman ....................................................... 117 Biodegradable Composite Polymer for Medical Application: A Review

Agus Haryono, Sri Budi Harmami ................................................................................................ 120 AdhF-36kDa Protein

Expression Pattern of Salmonella typhi on Different Environmental Conditions in Vitro I Nengah Kundera , Sanarto

Santoso , Aulanni’am, Sri Winarsih ................................................ 126 Evaluation on Antioxidative Activity of Pollard

Protein Hydrolysates Khothibul Umam Al Awwaly, Muhammad Halim Natsir , Abdul Manab ,Aris Sri Widati ............ 130

Exogenous IL-2 Promote T Cells Proliferation Under Anti –CD3/CD28 Combination Muhaimin Rifa'i
…………………………………………………………………………………………………………………….. 133

Potency of Inhibin B As Male Hormonal Contraception Candidates Based on Sperm Viability and LH 5
Serum Level of Rattus norvegicus

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Muhammad Hilman F. Amin , Aulanni’am , Agung P.W.Marhendra , Muslim Akmal ................. 5

134 Potency of Silicon Dioxide (SiO2) as

a Denaturator Media of Rabies Virus M. Misbah Khunur, Rizky Arief Shobirin, Muchid Abdul Aziz,

………………………………………………… 137 Decrease of Ethanol Content in Urine Wistar Rats after Giving Acute Alcohol Ni Made
Suaniti ........................................................................................................................... 141 Response of Rhizopus Sp in

Ration to the Food Consumption, Body Weight and Feed Conversion of Chickens Layer Grower Period of I Nur Alim

Natsir ............................................................................................................................ 144 Controlling Aedes Aegypti

Mosquito Population as DHF Vector with Sterile Insect Technique in Pasar Jumat – Nuclear Research Center Siti
Nurhayati, Budi Santoso ......................................................................................................... 147 vii Radiation Induced

Micronuclei in Lymphocyte Cell of Radiation Workers So ati Purnami, Yanti Lusiyanti, Masnelli Lubis, Dwi Ramadhani,

Devita Tetriana, Siti Nurhayati, Mukh Syaifudin........................................................................................................... The

Antioxidant Activity of Purple Star Apple (Chrysophyllum cainito L.) Tagor M. Siregar, Herry Cahyana, Ika Narishantika

.....................................................................

The In uence of Status and The Patterns of Driving License Ownership toward The Gradation of 10

Head Injury

Suffered By Motorcycles Accident Patients in Emergency Department of Saiful Anwar General 10

Hospital

Tri Maharani……………………………………………………………………………………………………………………….. Isolation of Active Extract of

Tengkawang Seed Oil (Shorea sumatrana Sym.) as Antibacterial Valentina Adimurti Kusumaningtyas, Ahmad Sulaeman,

Yusnelti, Dewi Meliati Agustini........... The In uence of Citrus Nobilis Lour Aqueous Fraction on Mice Fertilization Rate by
In Vitro Fertilization Method Widjiati, Raden Roro Wulan Oktavia Aryaguna Putri, Diah Kusumawati ....................................

Acoustic Performance Study of Human Hearing System Due To Noise Potential Damages Wike Herawaty, Nurida

Finahari .................................................................................................. Isolation and Characterization of A Gene Involved in

DNA Repair of the Extremely Radioresistant Bacterium Deinococcus Radiodurans Zubaidah Alatas

........................................................................................................................... Part B. Environment Section 152 157 163 164

167 170 175 B.1. Oral Presentation 182 - 299 Utilization of Bagasse Fly Ash for Nickel and Chromium Immobilization in

Cement-Solidi ed Metal Plating Sludge Ahmad Husni, Angga Dheta Sirajuddin Aji, Yusi
Arisandi……....................................... Alkaloids from Marine Sponges: Isolation of a New Aaptamine Related Compound

from an Indonesian Aaptos Aaptos Ajuk Sapar, I Wayan Mudianta, Andi H. Alimuddin, Mary J.

Garson…………………………................... The Effect of Refugia Blog to Spatial and Temporal Distribution of Natural Enemies in
Rice Field Asyik Nur Allifah AF, Bagyo Yanuwiadi, Zulfaidah P. Gama, Amin S. Leksono..............................

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Airborne Measurements of Particle Concentration from Biomass Burning in Northern Territory 3

Australia

Arinto Yudi Ponco Wardoyo…………………………………………………………………………………………………….. Zeolite Carbon Composite

Polyvinylchloride-Coated Wire Electrode for Lead Detection as an Environmental Sensor Atikah, Qonitah F , Chasan Bisri ,
Bagus Setiawan , Tyas Karya P………………………………………………

Geochemical Fractions of Heavy Metals in the Sediment of 39

Brantas River Downstream Barlah Rumhayati, Enco Sukarsa ………………………………………………………........................................ A

Preliminary Study: Towards Molecular Phylogeny of Endiandra (Lauraceae) Deby Ari ani, Adi Basukriadi, Mien A. Rifai ,
Tatik Chikmawati ……………………………………………...... 182 187 192 196 197 202 205 viii The Adsorption Desorption of

Ag(I), Pb(II), Cu(II), Cr(III), Ni(II) Toward Mercapto -Silica 8

Hybrid by Coloumn System Dwi Rasy Mujiyanti, Nuryono, Eko Sri Kunarti …………………………………........................................

Synthesis and Ability Test of Mg/Al Hydrotalcite-like from Arti cial Brine Water to Remove Eosin 17
Yellow

Eddy Heraldy, Sri Juari Santosa, Karna Wijaya, Triyono ……………………….…............................... Optimation of Bioethanol

Production from Sugar Cane Baggase using Separated Hydrolysis and Fermentation Method Based on Cellulase System

of Bacillus Circulans Evi Susanti, Neena Zakia, Sumari……………………………………………………..........................................

Conversion of West Kalimantan’s Tropical Peat into Bioethanol as Renewable Energy Ferry Hadary

…………………………………………………………………………………………………………………………..

Synroc Performance for Immobilization of High Level Liquid Radioactive Waste Gunandjar 9

……………………………………………………………………………….................................................

Bambara Groundnut (Vigna subterranea (L.) Verdc) as Sustainable Food: 37

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Strategic Consideration Future Prospect Hayyu Febriani , Endah Sri

Redjeki…………………………………………………………………..........................

Damping Concentration Effect Simulation on the Particulate PM10 Average Dynamic Dose Rate per 3
Year in X Region at Serpong Residency Icuk Setiyawati, Abdurrouf,

Eko Pujadi, P.A Saraswati …………………………………………................. Determination of Optimum pH and Adsorption Time of

Malachite Green on Coal Fly Ash M. Lathifurrijal, Anton Prasetyo, Diana Candra Dewi ……………………………………………................

Groundwater Mapping in Coastal Plain, North Kelantan –

Malaysia Nur Islami, Samsudin Hj Taib, Ismail Yusoff 42

………………………………………………………………. The Application of The Gompertz, Richards and Hyperbolastic Growth Models to
Describe Weight Growth NW Surya Wardhani, Solimun, Era Setiarini…………………………………………………….........................

Nitrogen Leaching on Coffee Based Agroforestry Systems in the Presence of Different Shade Trees R. Priyadarshini, K.

Hairiah, D. Suprayogo, J.B.Baon …………………………………………........................

Functional Morphology of Deposit Feeder Bivalve Theora lata (Bivalvia: Semelidae) 8

Reni Ambarwati, Trijoko …………………………………………………………………........................................ Soluble Quinone Reductase from

Methanosarcina acetivorans binds a Flavin Adenine Dinucleotide Suharti

……………………………………………………………………………………….............................................. Monitoring for the Effect of Chlorination in

Drinking Water by Using

Solid-Phase Spectrometry Sulistyo Saputro, Kazuhisa Yoshimura, Kô Takehara, Shiro Matsuoka, 26

Narsito

...……….............. Zoning on Vulnerability Potency of Geology Disaster Area in Malang-East Java Indonesia by Means of

Geophysics Data Sunaryo……………………………………………………………………………………................................................. 206 211 216

222 227 233 238 242 245 249 253 257 262 266 270 ix Development of Radionuclide Monitoring Stations in Indonesia in

Anticipating the Increasing of Peaceful Uses of Nuclear Energy in ASEAN Region - An Initial Assessment Susilo Widodo,

Syarbaini, Dadong Iskandar, Bunawas ………………………………........................... Diversity, Composition, and Community

Structure of the Teak Canopy Arthropods in Porang Crop Area Saradan Forest, Madiun, East Java Syahrudin Agung

Permana, Amin Setyo Leksono, Bagyo Yanuwiadi………………………………………… Biology of Fungi’s Pelawan Tree (Tristaniopsis

Merguensis) Triadiati, Anastastia Raditya Hidayanti, Nampiah Sukarno.......................................................... Mathematical

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Model of Interactions Immune System with Micobacterium Tuberculosis Usman Pagalay, Marjono, Kusworini Handono,

Agus Suryanto ..........……………......................... Role of Wild Plant as Alternative Habitat for Natural Enemies in Agricultural

Land Wiwin Maisyaroh, Bagyo Yanuwiadi, Amin Setyo L., Zulfaidah P.Gama …………………...................

Biosorption Phenomena of Heavy Metals and Radionuclides by Dispersed Bacterial Extracelular 9

Polymeric Substances Zainus Salimin, Endang Nuraeni

................................................................................................. 275 280 281 282 290 294 B.2. Poster Presentation The Study

of Phosphate Released from Aquatic Sediment and Its Effect on Algal Growth Asep Saefumillah, Nining Betawati

Prihantini, Dini Damayanti, Zaenab Sahamiddina…………… 300 - 366 300 Normative Law Dimension Introduction of Variety
Description for Pests and Plant Disease in Sustainable Food Crop Cultivation Bambang

Sudjito…………………………………………………………………………............................................. 301

Approach Method Self Organizing Maps (SOM) for Grouping Zone Season (ZOM) District Ngawi and 11

Accuracy Evaluation Method ZOM with General Regression Neural Network (GRNN)

Bambang Widjanarko Otok, Muhammad Sjahid Akbar, Agnisa Bhakti Persada ..……………....... 307

Biosorption of Lead(II) And Cadmium (II) by Biomass 8

of Azolla Microphylla-Silica in Continuous System Danar Purwonugroho, Sri Wardhani, Darjito, Deasi Ari Shandi ,

Descaniati Chan……………........ 312

Variation Shape and Color of Gland from Genus Orophea (Annonaceae) in Purwodadi Botanical 21
Garden

Dewi Ayu Lestari ……………………………………………………………………………........................................... 315 Single Nucleotide

Polymorphism Identi cation of Porang in East Java Estri Laras Arumingtyas

………………………………………………………………………………………………………….. 319 Logging Impact to Diversity of Epiphytes

at Malinau Research Forest (MRF)-CIFOR Malinau Regency 12

Ismail, Akas Pinaringan Sujalu……………………………………………………………………............................... 320 The

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Comparative Air Analysis of the Institute of Chemistry, UP Diliman using Passive Sampler with
7
Activated Charcoal and Commercially Available Adsorbent Lilibeth dlC. Coo , Christian Mark G.

Salvador , Abegail Z. Rasco………………………………................

325 Synthesis and Characterization of Nickel(II) doped Calcium Tartrate Tetrahydrate x (CaxNi(1-x)C4H4O6.4H2O)

Single Crystals in Metasilicate Gel Using CaCl2 Made of Achatina fulica Snail Shell Waste Mohammad Misbah Khunur,

Muchamat Chasan Basori, Yuniar Ponco Prananto, Rachmat Triandi Tjahjanto

……………………………………………………………………………………………………… Synthesis and Characterization of Mangan(II) doped

Calcium Tartrate Tetrahydrate (CaxNi(1-x)C4H4O6.4H2O) Single Crystals from Achatina fulica Snail Shell Waste

Mohammad Misbah Khunur, Imam Sakdi, Yuniar Ponco Prananto, Rachmat Triandi

Tjahjanto………………………………………………………………………………………………………… Epiphytic Orchid Diversity in Wilis Mountain,

District of

Ponorogo, East Java Nina Dwi Yulia, Sugeng Budiharta 29

…………………………………………………………………………………………..... Study of Infrared Spectra in

the Interaction of Cr(VI), Mn

(II), Ni(II), Cu(II), Zn(II), Cd(II), Hg(II), and Pb(II) 20

by Seaweed (Gracilaria Sp) Biomass Noer Komari , Filomena Matilda

…………………………………………………………...................................... Evaluation of Accuracy Clustering Zom BMKG in Ngawi with
Multivariate Adaptive Regression Spline (MARS) Approaching Nur Faizah, Bambang Widjanarko Otok, Sutikno

........................................................................ The Effect of Cadmium as Reductor and Reduction Time in the

Measurement of NO2 Gas in Ambient Air using KI-Amylum Absorber Qonitah Fardiyah, Hermin Sulistyarti, Nur Hayba
Islamiyah ..................................................... The Optimization Time of Steam Distillation of Vacuum Oven Dried

Aceh Patchouli Leaves (Pogostemon cablin Benth) and The Characterization by TLC and 6

GC – MS Sentot Joko Raharjo, Rurini Retnowati ...................................................................................... Potencies of Plants

around Fishes Ponds in Karang Sentul Village, Gondang Wetan District Pasuruan, East Java Solikin
………………………………………………………………………………………………………………………………………... Woody Plant Diversity and Structure of

Coffee Agroforests in the Recharge Area of Some Springs in Ngantang Subs District,Malang Regency, East Java Titut
Yulistyarini, Zaenal Kusuma, Soemarno, Endang Arisoesilaningsih................................... 330 334 338 342 346 352 356

361 362 Part C. Advance Technology Section C.1. Oral Presentation 367 – 485

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Analizing The Double Pulse Experiment by Using Fourier Transformation Abdurrouf 3

.................................................................................................................................. Dominant Frequency Analysis and

Seismic Vulnerability Index in subdistrict of Klojen, Malang Regency, using "Horizontal to Vertical Spectral Ratio" for
Seismic Microzonation Adi Susilo, Dwi Wahyudi............................................................................................................. Zircon
Imprinted Polymer: Synthesis, Characterization and Zircon Ion Absorption Properties Aladin Sianipar, Amran M.B.,

Buchari, I Made Arcana ................................................................. Effect of Pyrrole (Py) Concentration and the Number of
Cycles during Electropolymerization of Pyrrole (Py) on Platinum Electrode Anceu Murniati, Buchari, Suryo Gandasasmita,
Zeily Nurachman …………………………...….… 367 372 380 386 xi Development of Biomass Stove by Using Brick Refractory

Castable Insulator Bambang Poerwadi .....................................................................................................................

Veri cation of Absorbed Dose Rate To Water Determination For A Co-60 Gamma Beam Using Farmer Dosemeter and

Ptw Unidos in Ulin Hospital, Banjarmasin C. Tuti Budiantari, Nurman Rajagukguk

...................................................................................... Assessing Mud Volcano Vulnerable Area Using Fuzzy Approach
Candra Dewi ................................................................................................................................ Sensitivity Enhancement of

Enzyme Linked Immunosorbent Assay (ELISA) for Salmonella Detection Using Multi Walled Carbon Nanotubes
(MWCNTS) Dyah Kinasih Wuragil, Sukunya Oaew, Verasak Surareungchai .................................................. Standardization
for Gamma Emitting of Co-60 And Cs-137 Mixed Source Using Gamma Spectrometry Method Gatot Wurdiyanto,

Hermawan Candra, Wijono .......................................................................... The Diet Effect Using Amorphophallus Sp Raw
Flour

from East Java on Cholesterol of Rat (Rattus Novergicus) Wistar Strain 13

Harijati N, Widyarti S, Azrianingsih R ......................................................................................... Study on Formation of Fatty

Acid Methyl Esters (Fames) From Oil Seed Nyamplung (Calophyllum Inophyllum): The Effect of Catalyst Homogenous
and Heterogenous Hendro Juwono, Triyono, Sutarno, Endang Tri Wahjuni ………………………….………………………….. Preparation of
152Eu Standard Source in Al2O3 Matrix Hermawan Candra, Gatot Wurdiyanto, Pujadi

........................................................................... Estimation Parameters of Regression Spasial Lag by Ordinary Least

Square Method Indah Rahmawati, Sri Harini, Abdul Aziz .................................................................................... Estimation

Parameters of Regression Spatial Error by Estimated Generalized Least Square Method Lailiatul Mubtadiah, Sri Harini,
Abdul Aziz ................................................................................... The Determination of Skin Resistance Coe cient of
Apples Respect To O2 and CO2 Exchange Using SF6 Gas as a Tracer By a CO2 Laser Photoacoustic Spectroscopy

Method M.A.J. Wasono…………………………………………………………………………………………………………………………. Effect of Annealing on

the Crystallization and Hysteresis Loop of Pt/TiO2/Si(100)/PZT/Au Structures Masruroh
..................................................................................................................................... Resrad Build Code as Supporting Tool to

Estimate Radiological Impact from A Concrete Building Material Moekhamad Al yan

.................................................................................................................... Microparticulated Whey Protein Concentrate
(Wpc)-Low Metoxyl Pectin (Lmp) Complexes under Different Acidic Environment: A Rheology and Confocal Laser

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Scanning Microscopy (Clsm) Studies Mokhamad Nur, Todor Vasiljevic, Nicoleta Dragomir
………………………………………....................... Comparison of Three Spatial Analyses in Randomized Complete Block Design

Mohammad Masjkur ……………………………………………………………………………………………………………… 392 396 400 405 409 413 418 423
427 431 435 439 443 448 449 xii

Laboratory Measurements of Electrokinetic Potential to Determine The Fluid Flow Velocity in 22

Porous Media

at Unhas Campus, Tamalanrea, Makassar Muhammad Hamzah

Syahruddin................................................................................................ Polymerization of β-Pinene using Zirconium β-
diketonato Catalysts Muhammad Yusuf, Yessi Permana, Ismunandar .........................................................................

Standardization of 129I by Photon-Photon Coincidence Method with 125I Tracer Pujadi M, Gatot W, Hermawan C
................................................................................................. MLP Radial Basis Functions for Forecasting of the Chaotic

McGlass Time Series Samingun Handoyo.......................................................................................................................

Sample Preparation Technique for Total Arsenic in Tuna Fish Tiny Agustini Koesmawati, Buchari, Aminuddin Sulaiman,
Slamet Ibrahim, Kevin A. Francesconi .................................................................................................................... Test Rate of

Evaporation and Oxidation Stability of Synthetic Sex Pheromone Candidate Molecules Engineered Alpha Linolenic Acid
(ALA) of Basil Seed Oil (Ocinum basilicum L.) Warsito, Jumina, Chairil Anwar
……………………………………………………………………………………………… Study of Structure and Magnetic Properties of Complex

Compounds of Cu (II) with Nitronil Nytroxide and Dicyanamide Ion Ligands I Wayan Dasna
............................................................................................................................ Characteristics Test and Identi cation of
Radiation Sources from the High Performance Radioisotope Identi er 75023 using Co-60, Cs-137 and Eu-152 Wijono,

Gatot Wurdiyanto, Pujadi ............................................................................................. Study on Vertex Orders of Almost

Moore Digraphs with Self-repeat Yus Mochamad Cholily………………………………………………..………………………………………………………….

453 456 460 465 470 474 479 483 488 C.2. Poster Presentation 491 – 624 Optimization of Biodiesel Synthesis by
Trans-Esteri cation of Jatropha Curcas Oil with High Free Fatty Acid Aman Santoso
.............................................................................................................................. 491 Re nement Optimization of Crude Palm

Oil (CPO) using Zeolite or Clay and Its Potential As Biodiesel Raw Materials Aman Santoso, Sri Kumalaningsih, Susinggih
Wijana, Imam Santoso ......................................... 496 Analysis and Numerical Solution of Saint Venant Equations for the
2D Shallow Water Wave Models with Initial Value Problem and Boundary Value Problems Ari Kusumastuti, Dewi Erla M.,

Silva Ahmad A. ............................................................................ 501 Spatial Pattern Analysis of Dengue Fever Incident for
Early Warning Disaster Information in Surabaya City Arrowiyah, Sutikno, Setiawan
...................................................................................................... 505 Preliminary Study of Tra c Marking Formulation

Using Natural Rubber Grafted Modi cation Athanasia Amanda Septevani, Agus Haryono, Dewi Sondari , Ahmad Randy
…………………………… 510 xiii

Evaluation of Clustering Season Zone in Ngawi – East Java Using Support Vector Machine (SVM) 18
Bambang Widjanarko

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Otok, Agus Suharsono, Elly Nur Shobihah ............................................. Performance Genetic Algorithm and Simulated
Annealing of Case Studies Flow Shop Scheduling Dian Ratnawati …………………………………………………………………………………….

…………………………………. Development of Static Load Sensor Based on PVDF Film Element Didik R.
Santoso............................................................................................................................. The Pro le of the Constituents of
White Turmeric Essential Oils (Curcuma zedoaria (Berg) Roscoe) in the Variety of Distilate Collection Time Dore Yulia,

Rurini Retnowati, Moh. Farid Rahman ...................................................................... Nutrient Status Improvement of Ca and
Mg in Leaf by Administering Dolomite to Decrease Acidity Levels of Tangerine Fruit (Citrus suhuiensis Tan) Edi Siswadi,
Ari n, Syekhfani, Sudarmadi Purnomo....................................................................

Developing of Solid Phase Extraction Based on Zeolites: Case Studied Cr(VI) 2

Eti Rohaeti, Betty Marita Soebrata, Putri Ratna Furi, Irmanida Batubara.................................... 2
Synthesis of

Silver(I) Nitrate with Ethylenethiourea and Characterization Using SEM-EDAX Fariati, Markus Diantoro, Rokiy

Alfanaar...................................................................................... The Structure of Silver (I) Cyanate and Triphenylphosphine,

[AgNCO(PPh3)]4 Fariati, Effendy, Lilik Sunaria ........................................................................................................
Design,Implementation andAnalysis

Performance of XEN Hypervisor Virtual Machine Monitor Based on Linux for Server Technology 13
Solutions in Indonesia

Hendarmawan ………………………………………………………………………………………………………………………… Study of Phosphate Coated Wire

Electrode Using Chitosan as an Active Material Hendry Iyabu, Hermin Sulistyarti,

Atikah……………………………………………………............................... Flow Injection Multichannel Sensor for Nitrate Analysis Hermin

Sulistyarti, Atikah, Setyawan P. Sakti, Spas Kolev, R.W. Cattrall ................................. Testing of Radiation Exposure
Homogenity of 137Cs Source Spherical Geometry Holnisar, Rosdiani,

Pujadi............................................................................................................. Comparison of Amobilization Chrome (Cr) in

Waste of electroplating with Portland Pozzolan Cement - Kaolin and Portland Pozzolan Cement - Zeolite Use

Solidi cation / Stabilization Technique Ika Setyorini, Lies Wuryanita Adriyani

......................................................................................... The In uence of Inoculate Concentration Ratio of

Lactic Acid Bacteria Streptococcus thermophilus, Lactococcus lactis and Lactobacillus 33

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bulgaricus toward The Time of pH Decrease and The Weight of Curd in Cheese Production Marissa Agnestiansyah,
Chanif Mahdi, Anna Roosdiana ........................................................... Effect of Carbonization Temperature on Physical

Properties of Coconut Shell Carbon

Meytij Jeanne Rampe, Bambang Setiaji, Wega Trisunaryanti, Triyono 40

....................................... Analysis of Dinamic Behavior on Chua's Circuit Moh Anas Kurnia R, Erna Apriliani
............................................................................................... 514 519 520 521 525 530 535 538 541 542 543 547 550 551

555 560 xiv Immobilization Lipase from Mucor miehei using Silica Gel Matrix Ninik Afrizatus Sholichah, Anna
Roosdiana, Sutrisno ..................................................................

Existence of Global Solutions to a System of Nonlinear Klein-Gordon 36

Equations Ratno Bagus Edy Wibowo.............................................................................................................

Ranked Clusterability Model of Dyad Data in Social Network R. B. Fajriya Hakim, Subanar, Edi 19
Winarko

................................................................................... Analysis and Numerical Solutions of 2D Navier-Stokes for Model of

Shallow Water Flow with Initial Value Problem and Boundary Value Problem Ririn Kusumawati, Ari Kusumastuti, Fitriyanti

Rumfot, Silva Ahmad A........................................ The Optimum Time of

Steam Distillation of Wilted Aceh Patchouli Leaves (Pogostemon cablin Benth) and the 6
Characterization by Thin Layer Chromatogaphy and Gas Chromatography – Mass Spectra

Rurini Retnowati, Sentot Joko Raharjo ………………………….……………………………………………………….. Investigation the Mediating

Variable: What Necessary? Solimun.........................................................................................................................................

Study of Impregnated Co(II) Content in Zeolite-Co(II) Catalyst toward Phenol Oxidation Sri Wardhani, Tutik Setianingsih,
Agustina A Rahman............................................................... Recent Seismicity at Guntur Volcano Complex, West Java,

Indonesia Sukir Maryanto………………………………………………………………………………………………………………………... Isolation of α-Linolenic

Acid from Fatty Acids of Basil Seed Oil (Ocimum Basilicum L.) using Urea Inclusion; Variation of Fatty Acid-Urea Ratio

and Temperature Crystallization Suleman Duengo, Rurini Retnowati, M. Farid Rahman

............................................................... Synthesis and Characterization of Zinc-Soap Based-on Crude Palm Oil Sutrisno,
Irma Agustina, Ratih Purnamasari ................................................................................ The Effect of Alternative Nitrogen

Sources on Xylanase Production from Trichoderma Viride Sutrisno, Anna Roosdiana, Widyasari S

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........................................................................................ Effectivity of Ammonium Iron (III) Hexacyanoferrate

Decontaminant in eliminating 137Cs Radionuclides from the Body of Long Tail Monkey (Macaca fascicularis) Tur

Rahardjo …………………………………………………………………………………………………………………………… Preparation of Lead(II) Coated Wire

Electrode Using Chitosan Membrane for Monitoring Lead(II) in Water Samples Wiwin Rewini Kunusa, Atikah, Hermin
Sulistyarti…………………………………………………………………….. ERRATUM Health – Poster Presentation Testosterone Level of Male

Cattle at Regional Arti cial Insemination Center in Airlangga University, Faculty of Veterinary Medicine Sri Pantja
Madyawati, Pudji Srianto, Trilas Sardjito…………………………………………………………………… Advance Technology – Poster
Presentation Identi cation of Clay Minerals by Using

Differential Thermal Analysis (DTA) and Fourier Transform Infrared (FTIR) 34

Rachmat Slamet Santoso …………………………………………………………………………………………………………. 566 572 575 581 585 590 593
595 596 601 607 611 617 618 620 xv Advance Technology – Oral Presentation

Face Detection Image Based RGB Color Using Cube Form 35

Muh. Arif Rahman ..………………………………………………………………………………………………………………………. 625 Environment – Oral

Presentation

Airborne Measurements of Particle Concentration from Biomass Burning in Nothern 3

Territory

Arinto Yudi Ponco Wardoyo …………………………………………………………………………………………………………. 629 xvi

Proceedings of The International Conference on Basic Science 2

17-18 Feb 2011 ORAL The Simple Anti-oxidant Compound in Anti-cancer Potential Fraction of Kesum Leaves Extract
Muhamad Agus Wibowo 1, Lucy Ariani 2, Aulanni‘am 3, M. Aris Widodo 4, Basuki B. Purnomo 5 (1

,2) Chemistry Department, Science Faculty, Tanjungpura University, 27

Pontianak (Aguso2@yahoo.com)

(3) Chemistry Department, Science Faculty, Brawijaya University, 27

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Malang (4,5)

Department of Medicine, Faculty of Medicine, Brawijaya University, 43

Malang Abstract Kesum (Polygonum minus L) is a plant that potentially be used in the treatment of cancer. Some
researchers claim that the extract of this plant is capable of killing cancer cells and act as antioxidants. Recent research

shows that n-hexane fraction of Kesum leaves have anti-cancer activity. This fraction demonstrates a proliferation
activity inhibition of lung cells that exposed by benzapyrene. In this study, isolation of potential compound as anticancer
that contained in the n-hexane fraction of Kesum leaves extract has been carried out. The analysis of isolated

compound was carried out by using GC-MS spectroscopy. The results showed that 2-hydroxy-2-methyl-4-pentanone is a
dominant compound that contained in the fraction of n-hexane and it has antioxidants activity in the DPPH test. Based
on these result, 2-hydroxy-2-methyl-4-pentanone is assumed to be the compound that has potency as an anti-cancer.

Keywords: kesum, anti-oxidant, 2-hydroxy-2-methyl-4-pentanone. 1. Introduction Kesum (Polygonum minus L) is one of

the typical and popular plants in West Kalimantan, which are used as additives in various typical foods [1]. This plant is

potentially be used to treat cancer. The anti-oxidant test of the aqueous extract of Kesum by using the method of
potassium ferricyanide, FTC (ferric thiocyanate), and TBA (thiobarbituric acid) showed that water extract of Kesum are
powerful antioxidants [2]. Vimala (2008) in his research stated that the methanol and water extracts of the Kesum

leaves are powerful antioxidants [3]. The anti-cancer activity is owned by Kesum leaves, too. Mackeen et al. (1997) in his
research stated that the ethanol extract of Kesum leaves are cytotoxic to HeLa cells, with LC50 (lethal concentration 50)
is 30µg/ml [4]. The n-hexane fraction of Kesum leave is a potential fraction to inhibited cancer growth [5]. Wibowo et al.

(2010) in his study states that this fraction is able to increase p53 gene expression,

decreased the levels of MDA (malondialdehyde), and reduce the proliferation activity of 16
the lung cells in

the animals model that exposed by benzopyrene [5]. Given that the n-hexane fraction of Kesum leaves is potential as a

lung cancer drug, it is necessary to isolate the anti-cancer potential compound in the n-hexane fraction. 2. Experimental
Details Materials Kesum (Polygonum minus L) used in this study were collected from Kota Baru, Pontianak. This
species is determined in the "Laboratory of Plant Taxonomy and Structure Development, Department of Biology,

Brawijaya University" and the specimen stored there. The parts of plants that used in this study are its leaves. The n-
hexane fraction (the fraction of potential anti-cancer) is obtained by using the method Wibowo et al. (2010) [5].

Determination of Anti-oxidant compounds by Thin Layer Chromatography method (TLC) The n-hexane fraction was
developed by using methylene chloride. Test of anti-oxidant compounds made with spray

reagent DPPH (2,2- diphenyl-1-picrylhydra-zyl) 44

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by using thin layer chromatography method. Antioxidants potential stain was marked with pale yellow spots are left in
place for 10 hours. Determination of phenolic compound group was used the spray reagent of FeCl3 1%. Determination
of terpenoid compound group was used the spray reagent of vanillin 1% in sulfuric acid. 59

Proceedings of The International Conference on Basic Science 2

17-18 Feb 2011 ORAL Isolation and analysis of antioxidant compounds from n-hexane Isolation of potential anticancer
stains performed gravity column chromatography (KKG) using a solvent gradient from non-polar to polar, to obtain
relatively pure compound. Against the pure compounds obtained and then

analyzed by using gas chromatography-mass spectroscopy (GC-MS). 3. Result and 41

Discussion This study begins with the determination of the dominant spots that are anti-oxidants by using the method
of TLC (thin layer chromatography). Determination of antioxidant stain performed using DPPH spray reagent 0.2%. In

addition, also tests the classi cation of compounds by TLC method. Determination of phenolic compound group was
used the spray reagent of FeCl3 (1%) while the terpenoids was used the spray reagent of vanillin (1% in sulfuric acid).
The results of this experiment are presented in Figure 2 and Table 1. The results showed that the rst spot (Rf 0.95) is a

anticancer potencial. It is shown by the formation of yellow-pale color when tested with DPPH spray reagent. The rst
spot is a terpenoid compound that shown by the formation violet color when tested by vanillin spray reagent. In addition
of terpenoids, the phenolic compounds are also found (spots 9, 10, and 11) that is potential as anticancer compounds.

Figure 1. TLC determination of antioxidant spots. (A) visible light; (B) 1% FeCl3; (C) vanillin in H2SO4; (D) 0.2% DPPH in
methanol (developed n-hexane fraction by using MTC solvent). Isolation of the dominant spot of anti- oxidants ( rst
spot; Rf 0.95) is done by using the column of chromatography and the gradations of solvent from a non-polar to polar.

Isolates were then analyzed by GC-MS instrument. The structure of compounds in each peak was con rmed by an
internal standard.. The result of analysis is given in Figure 2 and Table 2. Table 1. The Rf of chromatogram as a results
of elution by using methanol. No light FeCl3 Vaniline DPPH Rf Comp. 1 yellow - crimson Yellow pale 0,95 terpenoid 2

green green green Green base yellow 0,87 Phenolic 3 brown - crimson - 0,76 terpenoid 4 green green green green 0,68
Phenolic 5 green grey green - 0,59 Phenolic 6 brown blue green - 0,43 Phenolic 7 brown blue green - 0,35 Phenolic 8

brown blue green - 0,30 Phenolic 9 brown blue dark Yellow pale 0,19 Phenolic 10 brown blue dark Yellow pale 0,05
Phenolic 11 brown blue dark Yellow pale 0 Phenolic Figure 2. Chromatogram analysis of isolates by using GC-MS Table
2. R time of the peak chromatogram on the GC-MS analysis Peak R Time Concentration (%) 1 7.824 72 2 16.227 10 3

19.697 18 Analysis by MS at the rst peak of the chromatogram gives the spectra as in the gure 3. The Analysis of
compounds by using an internal standard has showed that the target compound is 2-hydroxy-2-methyl-4-pentanone 60

Proceedings of The International Conference on Basic Science 2

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17-18 Feb 2011 ORAL Figure 3. The spectral of the rst peak (tr 7.824) from the chromatogram From the analysis above,
the compound of 2-hydroxy-2-methyl-4-pentanone is the dominant compound in the fraction of n-hexane that is as anti-
oxidants. The ability of compound 2- hydroxy-2-methyl-4-pentanone as anti-oxidants because of these compounds are

capable to delocalizations of electron. The existence of the 2-hydroxy-2-methyl-4-pentanone and the several of phenolic
compounds in the n-hexane fraction are allowing this fraction can reduce the

levels of MDA and increased the expression of p53 gene in 16

the lung tissue of the animal model in the research of Wibowo et al. (2010). 4. Conclusion From the results of research,

it can be concluded that the compound of 2-hydroxy-2- methyl-4-pentanone that contained in the fraction of n-hexane
are dominant compounds that has anti-oxidants activities and potential to be used as an anti-cancer. 5.

Acknowledgements Our thanks are to convey

to the Directorate General of Higher Education (DIKTI) that has funded this research 24

through research grants PHB in 2010 with a contract number: 3158/H22.13/PL/2010 (21 April 2010). 6. References [1].
Wibowo, M.A., R. Wulandari (2007), Isolasi dan uji Aktivitas Antimikroba Minyak Atsiri Daun Tanaman Kesum

(Polygonum cf minus Huds), Proceeding of Basic Science Seminar, University of Brawijaya, Malang [2]. Huda-Faujan, N.,
Noriham, A., Norrakiah, A.S. and Babji, A.S. (2007), Antioxidative Activities of Water Extracts of Some Malaysian Herbs,
ASEAN Food Journal, 14 (1): 61-68 [3]. Vimala, S., M. A. Ilham, A. A. Rashih, S. Rohana & M. Juliza (2008), Antioxidant

and Skin Whitening Standardized Extraxts: Cosmeceutical and Neutraceutical Products Development and
Commercialization in Firm, Forest Research Institute Malaysia FRIM), Selangor Darul Ehsan, 224-230. [4]. Mackeen
M.M., Ali A.M., El-Sharkawy S.H., Manap M.Y., Salleh K.M.; Lajis N.H., & Kawazu K. (1997), Antimicrobial and Cytotoxic

Properties of Some Malaysian Traditional Vegetables (Ulam), Pharmaceutical Biology, 35 (3), 174-178 [5]. Wibowo, M.A.,
S. Anwari, F. Rahman, Aulanni‘am (2010), Efek Antiproliferasi Ekstrak n-Heksan Daun Kesum (Polygonum minus) Pada
Sel Kanker Paru Tikus: Upaya Pemanfaatan Kesum Sebagai Obat KankerParu, Media Kedokteran Hewan, 26(1), 15-18 61

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