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Lazo
19B
Experiment 4: Identification of an Unknown through Qualitative Analysis 01/03/19
Objectives:
To use the qualitative tests to analyze an unknown sample.
To be able to identify the unknown sample after the qualitative tests
Prelab Questions
o Water START
o 5% NaOH
o 5% NaHCO
o 5% HCl
o Concentrated H2SO4
Is the
sample
Liquid Solid
solid or
liquid?
Add 1 to 2 drops
Use a spatula tip
Start
Soluble
Do a Litmus Test in Yes Nitrogen, Oxygen, or
No
water? compounds with until
4 carbons
Solubility in 5%
NaOH
No Yes
Solubility in 5%
Carboxylic acid or phenol
HCl Test
Solubility in
No Yes 5% NaHCO3
Test
Amine Yes
Solubility in No
H2SO4 Test
Phenol Carboxylic
acid
No Yes
Unsaturated
Ignition Test compounds: yellow
Unsaturated compounds vs Combustion flame and sooty
Saturated compounds smoke
Saturated
compounds: Yellow
flame, non-sooty
smoke
Haloalkanes Sn1 mechanism Formation of
Alcoholic Silver insoluble silver
Nitrate Test halides
Green Precipitates
Phenols Purple,
Iron (III) Chloride Test Phenols and enols red, or green
complexes
Procedure:
Solubility tests:
o Water START
o 5% NaOH
o 5% NaHCO
o 5% HCL
o Concentrated H2SO4
Is the
sample
Liquid Solid
solid or
liquid?
Add 1 to 2 drops
Use a spatula tip
1. Ignition test
Start
Start
Put 1-2 drops of liquid sample into a Add 4-5 drops of ethanolic silver
test tube or dissolve a spatula tip of nitrate test solution and observe for
solid sample in minimum amount of formation of gray/white precipitate.
ethanol and add 1-2 drops of If no precipitate forms after 5
solution to test tube minutes, heat mixture on steam
bath.
3. Baeyer Test
Start
Start
Start
Start
Start
Put 1-2 drops of sample in and Add a drop of 6M NaOH and heat
about 5-8 drops of hydroxamic test test tube in water bath until boiling
solution into a test tube
8. Iodoform Test
Start
Dissolve 2-3 drops of sample in 8-10 Add 8-10 drops of 5% NaOH and
drops of water in a test tube add KI/I2 solution drop-by-drop until
dark color persists. Shake solution
vigorously and observe formation of
precipitates.
Add 3-5 drops of 5% NaOH until Warm test tube in hot water bath to
color disappears and continue 60 ˚C if no precipitates form. If
shaking solution. Dispose in dark color disappears add KI/I2
halogenated organic waste disposal until dark color persists for 2
minutes
Start