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Culture Media by Shyamal PDF
Culture Media by Shyamal PDF
CULTURE MEDIA
-A source of energy
-Sources of carbon, nitrogen, sulfur,
phosphorus
-Minerals, e.g., Ca2+, Mg2+, Na+
-Vitamins and growth factors
- Water
pure
12/30/13 culture of S.albus Dr. Shyamal Kr Paul, Culture media
mixed culture 4
Culture Media
a. broth b. semi-solid
12/30/13 Dr. Shyamal Kr Paul, Culture media 12
c. solid
2. Plated
12/30/13 Dr. Shyamal Kr Paul, Culture media 13
Agar
Complex polysaccharide
Used as solidifying agent for culture media in
petri plates, slants, and deeps
Generally not metabolized by microbes
Liquefies at 100°C
Solidifies at 40°C
Basal/ordinary/general
Enriched: with enrichment substances
Enrichment
Selective media: with inhibitory substances
Differential media: with indicators/dyes
Special Media for Biochemical Testing
Media for Antimicrobial Susceptibility Testing
12/30/13 Dr. Shyamal Kr Paul, Culture media 15
Basic media
Plate
deep (Petri dish)
Slant
alpha/partial hemolysis
Enrichment Broths
“encourage” the growth of a particular type of
microbe;
Addition of “nutrients” enrich for microbial group
of interest
Ex. Cellulose broth- enriches for microbes
which degrade cellulose
Ex. Petroleum Broth- enriches for microbes
which could eat an oil spill.
12/30/13 Dr. Shyamal Kr Paul, Culture media 30
Selective Media
Differentiatial between
lactose fermenting and
non fermenting enteric
bacteria
Reducing media
Candle jar
CO2-packet
12/30/13 Dr. Shyamal Kr Paul, Culture media 43
Culture Media to Isolate Common
Members of Family Enterobacteriaceae
MacConkey agar
Indicator: phenol red
Inhibitors: bile salts, crystal violet
Carbohydrate source: lactose
LF= pink-red
NLF= clear/colorless
Typical organisms