Prokaryotes and Eukaryotes cell

Habtamu Biazin (BSc, MSc)

AAU ,CHS, SoM
Department of MIP

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 Prokaryotic and Eukaryotic cell
 All living cells can be classified as

 Prokaryotes cells: pre-nucleus

 the Greek words pro (before) and karyon (nucleus).

 All prokaryotes are:

 single-celled organisms, and all are bacteria.

 Microscopic

 cells lack a nucleus and other membrane-enclosed

structures.

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 Cell types …
 Eukaryotes cells-true nucleus

 Eu (true) and karyon (nucleus).

 Unicellular (microscopic) and multicellular

 cells have a true nucleus and

 membrane-enclosed structure or organelles

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 Cell types…
 Viruses: acellular, parasitic particles composed of
a nucleic acid and proteins.

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 Cell types …
 Eukaryotes include all

 plants, animals, fungi, and protists.

 Prokaryotic and eukaryotic cells are similar in several ways.

 Both are surrounded by a cell membrane, or plasma membrane.

 encode genetic information in DNA molecules.

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 Similarities and Differences Between Prokaryotic and Eukaryotic Cells
Characteristic Prokaryotic Cells Eukaryotic Cells
Genetic material (DNA) Single circular DNA Paired linear DNA

Location Nuclear region (nucleoid) Membrane-enclosed nucleus

Nucleolus Absent present
Histone proteins Absent Present
Extrachromosomal DNA Plasmids Mt and chloroplasts

Membrane bounded organelles Absent Present: ER, Mt, GA etc.

Respiratory enzymes Cell membrane Mitochondria

Ribosomes 70S 80S in cytoplasm and on rER,
Cell wall PG found on most cells Cellulose, chitin, mannan,glucan
Cell division Binary fission Mitosis and/or meiosis

Reproduction Asexual Sexual or asexual reproduction

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 Prokaryotes cells…
 Bacteria are prokaryotic, single-celled microorganisms.

 Have a complex cell wall and

 Have a single, Ds ,circular DNA

 lack a nuclear membrane

 Reproduce by Binary fission

 Metabolically they are active and mostly independent

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 Taxonomy
 Taxonomy:
 is the formal system for organizing, classifying,
and naming living things.
 Classification:
 is the assignment of organisms (species) based
on evolutionary relationships.
 Nomenclature:
 assigning specific names of organisms (genus
+species)
 Identification:
 Determining and recording traits of organisms
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for placement into taxonomic schemes. 7/11/2019
 The Levels of Classification:
Hierarchy

 It begins with domain and ends with species the

smallest and most specific taxon.

 Domain: archaea, bacteria and eukarya

 Binomial system:

 Assigning specific names

 It describes each living organism by two names: genus

and species.
 C. Linnaeus (1701-1778)

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 Classification …
 Binomial nomenclature:

i. Genus comes before species (e.g., Escherichia coli)

ii. Genus name is always capitalized (e.g., Escherichia)
iii. Species name is never capitalized (e.g., coli)
iv. Both names are always either italicized or underlined
( e.g Escherichia coli or Escherichia coli)
v. The genus name may be used alone, but not the species name (i.e
saying or writing “Escherichia “ alone is legitimate while saying or
writing “ coli” is not)

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 Classification…
 In general, Bacterial classification depends on:
 Phenotypic methods
1. Morphology and arrangement
2. Staining
3. Anatomic structures
4. Physiology
 Cultural characteristics
 Biochemical reactions
 Antigenic structure
 Genetic :Base composition of bacterial DNA
 DNA-DNA hybridization
 rRNA
 G+C Content

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 Morphology of Bacteria
Size
Prokaryotes are among the smallest of all organisms.
Most prokaryotes range from 0.5 to 2.0 um in
diameter.
With some exception
cyanobacteria are 60 um long
Bacillus anthrax measures 10um

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 Morphology of Bacteria
Shape
1. Spherical /circular Coccus/Cocci Plane of division
I. Pairs diplococci one
II. Four cells arranged tetrad two
III. Eight cells arranged Sarcina three
IV. Chains streptococci one
V. Clusters staphylococci Random

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  Arrangement of bacterial cells
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 Morphology of Bacteria
2. Rod –shaped Bacilli/bacillus
Bacilli divide in only one plane. Diplobaccilli or Streptobacilli
 Straight
 Curved/comma-shaped (vibrio) Vibrio
 tapered-fusiform
 Coccobacilli-short rods
 Pleomorphic-change shape and size

Bacilli

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 Morphology of Bacteria
3. Spiral shaped:
 Spiral bacteria are not generally grouped together.

 Spirillum - stiffed

 Spirochetes –flexible

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 Staining characteristics
 Color

 Bacteria are colorless, transparent(similar refractive

index with surrounding environment).

 Staining preparation needed to see them under the

microscope
 Gram positive vs. gram negative bacteria

 Acid fast positive vs. negative bacteria

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 Bacterial cell structure

Typical bacterial cell structure

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 Bacterial structure
 Bacterial structure

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 Bacterial cell Structure
1. Surface layers (capsule, loose slime)  External
2. Surface appendages (Pilli, flagella) structures

3. Cell envelope (Cell wall , Cell membrane )

4. Internal structures (nucleoid, cytoplasm, plasmid,

ribosomes, mesosomes, endospores etc.)

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 1. Surface appendages (Pilli, flagella)

 Are surface structures originated outside the CM

 Sometimes being attached to it, and

 Extended into the environment

 Two types of surface appendage are found on certain

bacterial species:

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 Surface appendages…
I. Flagella :
Are thread/whip like structures

Free at one end and attached to a cell at the other end

made of protein flagellin.
are organs of locomotion

 occur on both Gram-+ve and Gram-ve bacteria.

 Their presence can be useful in identification.

 Flagellar antigen-H antigen

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 Flagella…
 The flagella has 3 basic parts
 The filament-the long outermost region.
-Consists of flagellin a Globular protein.
The hook-where the filament is attached
-wider and consists of different proteins.
The basal body-which anchors the flagellum to the CW
and CM.
-composed of a small central rod inserted
into a series of rings.
-in GNB, it contains two rings
-the outer pair of ring is anchored to the
CW.
-the inner pair of rings is anchored to the
CM
24 -In GPB, only the inner pairs of rings are
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 Flagella…

25  Structure of prokaryotic 7/11/2019

 Surface appendages…
Flagellar arrangements

1. Atrichous: Bacteria with no flagellum.

2. Polar
2.1. Monotrichous: Bacteria with single polar flagellum.
2.1. Lophotrichous: Bacteria with bunch of flagella at one pole.
2.3 Amphitrichous: Bacteria with flagella at both poles.

3. Peritrichous: Bacteria with flagella all over their surface.

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 Flagelar arrangement

27 Typical arrangements of bacterial flagella. 7/11/2019

 A-Monotrichous; B-Lophotrichous; C-Amphitrichous; D-
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 Flagella…
 Function

 Locomotion/movement (motile vs. non-motile)

 Colonization/attachment

 Antigenic-flagellar antigen “H” antigen

 Serogrouping/serotyping

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 ii. Fimbriae and Pili
 Hairlike appendages common in GNB.
 Are shorter, straighter and thinner than flagella.
 Used for attachment and transfer of DNA.
 composed of protein called pilin.

 pili occur almost exclusively on Gram-negative bacteria.

 found on only a few GPB(e.g., Corynebacterium renale).

 Pili (Latin hairs), fimbriae (Latin fringes).

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 Pili and Fimbriae…
 Two types (Based on function)
 Common pili(Fimbriae):
 A few to several hundreds per cell.
 Have the tendency to adhere to each other and
to the surface.
 The structure for adherence to cell to cell
surface.
 They involved in biofilm formation.
 Help bacteria to adhere epithelial cells

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 Pili and Fimbriae..
 Sex pili:

 Are usually longer than fimbriae

 Only 1 or 2 in numbers per cell.

 Involved in motility and transfer of genetic

material during the process of conjugation.

 Twitching and gliding motility

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 Comparison between flagella and
Pili

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 Cell envelopes(cell wall, cell membrane )

 Cell wall:
 a rigid, multilayered structure

 Non -selectively permeable

 Almost exclusively common in all bacterium except

Mycoplasma spp., protoplasm
 Contain somatic antigen “O” Ag.

 Made up of a polymer of peptidoglycan (murein):

 N-acetyl glucosamine( NAG)

 N-acetyl muramic acid (NAM)

 Four aa attached on NAM as side chain

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 Cell wall…
 L-alanine
 D-alanine
 D-glutamic acid
 Diaminopimelic (DPA)-lysine
 A set of identical peptide bridge cross links NAG with NAM
with the help of carboxypeptidase and transpeptidase
enzymes.

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 Cell wall…
 Components of cell wall of Gram positive bacteria

1. Peptidoglycan
2. Teichoic acid

 Components of cell wall of Gram negative bacteria

1. Peptidoglycan
2. Lipoprotein
3. Phospholipid
4. Lipopolysaccharide

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 GPB vs. GNB Cell Wall

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 GPB vs. GNB Cell Wall

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 CELL WALL DIFFERENCE

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 Cell wall defective bacteria
I. Natural: e.g. Mycoplasma species
II. Induced:
 produced after exposure of antibiotics, detergents, lysozymes
1. Protoplasm:

a bacterial cell with its cell wall completely removed .

 It is spherical in shape and osmotically sensitive (gram +ve)
2. Spheroplast :

a relatively spherical cell formed by the weaking or partial

removal of cell wall component
 e.g by penicillin tx of gram –ve .
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 Cell wall defective bacteria
3. L-forms:
 are (G+ve and -ve mutant bacteria)

 partial or complete loss of the cell wall.

Resistance to antibiotics
Can grow and divide
Encounter infections
Can revert to the normal form

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 Functions of cell wall
1. Provides shape to the bacterium

2. Gives rigidity to the organism

3. Protects from environment

4. Provides staining characteristics to the bacterium

5. Contains receptor sites for phages/complements

6. Site of action of antibody, antibiotics and colicin

7. Contains toxic components to host-endotoxic

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 Cell /plasma membrane
 Phospholipid by layer +protein

 It accounts for 30% of the dry weight of bacterial cell.

 It is composed of
 60% protein

 20-30% lipids and

 10-20% CHO

Function of cell membrane

1. Selective permeable/barrier
2.Transport of molecules(passive, active)
3. Energy generation: Respiration –ATP
4. Synthesize biosynthetic enzymes
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 Surface layers
 Glycocalyx :

 Secreted substance on the surface of many

prokaryotic cells.

 A.k.a the pericellular matrix- substances that

surround cells

 Glycocalyx (meaning sugar coat)

 It is a viscous (sticky), gelatinous polymer

 It is composed of polysaccharide, polypeptide, or

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 surface layers
 Capsule:

 Glycocalyx which is organized and is firmly attached to the cell

wall
 the outer most layer of bacteria cell.

 made of polymers of polysaccharide +polyalcohols+aminosugars

except in B.anthracis (polymers of D-glutamic acid).
 capsulated bacteria produced smooth, mucoid colonies.

 Non-capsulated bacteria produced rough non-mucoid colonies.

 Capsular antigens, K,Vi

 e.g. Hameophilus influnzae ,S.pneumoniae, N.meningitis

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 function of capsule
 Anti-phagocytic Activity

 Antigenicity

 Attachment/adherence/colonization

 Resistance to drying

 Serotyping bacteria

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 Surface layer…
 Slime layer
Glycocalyx substance is unorganized and only
loosely attached to the cell wall.
The Glycocalyx is a very important component of
biofilms.

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 Mesosomes
 Infoldings/invagination of the plasma membrane in which

bacterial chromosomes attached to it.

 Often found near dividing line in bacteria

 Involved in segregation of newly replicated chromosomes

following DNA replication.

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 Internal structures

 Ribosomes

 DNA(Nucleoid)

 Granules
Cytoplasmic constituents
 Plasmid

 Endospores

Polyamine

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 Inclusion bodies /granules
 Aggregates of various compounds used as foods storage /reserves

 Poly-beta hydroxyalkanoate (PHA):

 is a derivative of poly-hydroxybutyrate (PHB): source of

carbon and energy

 Metachromic granules (volutin): inorganic sulphate, Sulfur

granules
 Polysaccharide granules : usually glycogen or starch

 Lipid inclusions

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 Endospores
 Small round, highly resistant, resting structures produced with the

cell during starvation/accumulation of toxic wastes.

 Little or no metabolism inside the spore

 Spores are resistant to heat, radiation, chemicals and desiccation

 Spores are cable of detecting their environment and under favorable

environmental conditions.

 they germinate and retuning to the vegetative state

 Spore formation (sporulation)-process of forming spores

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 Layers of endospore

 Core: the core is dehydrated cytoplasm; containing DNA,

ribosomes ,enzymes ,calcium dipocolinate (5-15% of dry

weight).

 Cortex: the cortex is a modified cell wall/PG layer.

 Coat: keratin-like proteins layers that are impermeable to

most chemicals

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 Structure of bacterial endospore
o Are formed in response to certain
adverse nutritional conditions.
o Are inactive bacterial cells.
o More resistant to desiccation, heat
and various chemicals.
o Contain calcium dipicolinate which
aids in heat resistance within the
core.
o Germinate under favorable
nutritional conditions.
o Helpful in identifying some species
of bacteria.
o Spore germinate to produce a single
vegetable cell.

Structure of bacterial endospore

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  Arrangement of spore/location

 Terminal

 Central

 Subterminal

 Spore former bacteria

Clostridium spp

Bacillus spp.

Coxiella burnetti
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 Germination of spores
 The overall process of converting a spore into a

vegetative cell.

 It has three stages: Activation, germination proper

and outgrowth.
Activation: favorable environmental condition

Initiation: degradation of the cortex PG and

release of calcium dipocolinate from the core.

Outgrowth: forming new vegetative cells
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 Germination…

A typical sporulation cycle from the active vegetative

56 cell to release and germination 7/11/2019
 Bacterial Nutrition and Growth
 Bacterial nutrition:

 Bacteria, like all cells, require nutrients for the

maintenance of

their metabolism and for cell division.

 Bacterial structural components and the

macromolecules for the metabolism are synthesized

from the elements.
 The four most important elements of bacteria are C,
H2, O2 and N2.
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 Minerals: sulfur and phosphorus, trace elements
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 Carbon
 Organisms require a source of carbon for the synthesis

of numerous organic compounds that comprise

protoplast.

 Depending on their requirements, bacteria can be

classified as

1. Autotrophs: Free-living, non-parasitic bacteria

 use carbon dioxide as carbon source.

 The energy needed for their metabolism can be

59 obtained from: 7/11/2019

 2. Heterotrophs:

 Parasitic bacteria

 require more complex organic compounds as their

source of carbon and energy.

 Human pathogenic bacteria are heterotrophs

 The principal source of carbon is CHO which are

 degraded either by oxidation, in the presence of

oxygen, or
 by fermentation, in the absence of oxygen, to
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provide energy in the form of ATP.
 Glycolysis
 Pyruvic Acid
 Fermentation
 Aerobic
respiration

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 The main types of energy-capturing metabolism.
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 Terms Relating to Energy and Carbon
Sources

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 Bacterial Nutrition…
 Hydrogen and oxygen

 Obtained from water.

 Essential for the growth and maintenance of cell.

 Nitrogen

 Constitutes 10% of dry weight of bacterial cell.

 Obtained from organic molecules like proteins and

inorganic molecules like ammonium salts and nitrates.

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 Growth factors
 Growth factors

are organic compounds

Required in small amounts

the cell can not synthesize from other carbon

source.
 These are aminoacids, purines and pyrimidines, and
vitamins.
 Prototrophs: Wild-type bacteria with normal growth
requirements.
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 Auxotrophs: Mutant bacteria, which require an
 BACTERIAL GROWTH
 It is an orderly increase in all the components of an

organism.

 It is an increment in biomass.

 It is synchronous with bacterial cell reproduction.

 Generation time: is the time taken for the size of a

bacterial population to be
 Bacterial growth phases
Lag phase
Exponential(log) phase
double.Maximal stationary phase
Decline phase

 The normal bacterial growth curve has four phases.

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 1. Lag phase
 The period of adaptation

 active macro molecular synthesis like DNA, RNA,

various enzymes and other structural components.

 It is the preparation time for reproduction; no increase

in cell number.

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 2. Exponential(log) phase

 The period of active multiplication of cells.

 Cell division precedes at a logarithmic rate.

 determined by the medium and condition of the

culture.

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 3. Maximal stationary phase
 The period when the bacteria have achieved their
maximal cell density or yield.
 There is no further increase in viable bacterial cell
number.
 The growth rate is exactly equal to the death rate.

 A bacterial population may reach stationary growth when

one of the
following conditions occur:
 The required nutrients are exhausted
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 Inhibitory end products are accumulated
 4. Decline phase
 The period at which the rate of death of bacterial cells

exceeds the rate of new cell formation.

 There is drastic decline in viable cells.

 Few organisms may persist for so long time at this

period at the

expense of nutrients released from dying micro-

organisms.

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 Factors influencing bacterial
growth

 Rates of bacterial growth are influenced by the

following environmental parameters.
I. Nutrition
II. Temperature
III. Oxygen

IV. PH

V. Salinity

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 1. Nutrition
 Macromolecules (source of C,H,O,N)

 Minerals: sulfur and phosphorus, trace elements

 Growth factors: amino acids, purines, pyrimidines and

vitamins.

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 2. Temperature
 Optimal growth range of temperature:

 The temperature at which the maximum growth rate

occurs; and results in the shortest generation time of

bacteria.

 Optimal growth temperature

 Psychrophilic bacteria:15-20 ° c; grow best at low T°

range

 Mesophilic bacteria:30-37 °c; grow best at middle T°

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range
 Optimal temperature required of bacteria
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 3. Oxygen
 Base on oxygen requirements and tolerance,
bacteria are classified as:
1. Obligate aerobes
2. Microaerophilic
3. Obligate anaerobes
4. Facultative anaerobes
5. Aerotolerent anaerobes
6. Capnophilic

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 Obligate aerobic bacteria grow only when free oxygen is

available to support their respiratory metabolism.

 They obtain ATP by using oxygen as a final electron

acceptor in respiration.

 Obligate anaerobic bacteria grow in the absence of oxygen;

exposure to oxygen kills anaerobes.

 FA bacteria: grow in the presence or absence of O².

 They obtain ATP by fermentation or anaerobic respiration.

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 Environmental oxygen…
 Microaerophilic bacteria grow best at reduced

oxygen tension; high oxygen tension is toxic to

them.
 Aerotolerant anaerobes do not utilize oxygen

but can survive in its presence.

 are not killed by oxygen, because they possess

alternative mechanisms for breaking down

peroxides and superoxide.
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 Capnophilic bacteria grow best at a higher
 Environmental oxygen…

Bacterial growth at different environmental oxygen

concentration
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 4.Hydrogen ion concentration
 Neutrophilic bacteria grow best at near neutral PH

value.

 Acidophilic bacteria prefer to grow at low PH value

(acidic medium).

 Alkalophilic bacteria prefer to grow at high PH value

(alkaline medium).

 Most pathogenic bacteria grow best at PH of 6-8.

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 5. Salinity
 Salt content of the medium affects bacterial growth.

 Halophile bacteria grow best at high salt

concentration.

 Moderate halophiles: require 3% salt concentration.

 Extreme halophiles require 15% salt concentration.

 Most bacteria can not tolerate high salt concentration.

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 6. Osmotic pressure
7. Sun light

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