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2-1. Obtain 20 measurements from two different organisms of the same species. e.g., the lengths of
20 mature leaves from each of two plants of the same species, or the diameters of toes/fingers from
2 people. Put data into 2 digits (e.g., 34 mm). Calculate, for each individual, these statistics:
1. COUNT (n) =
2. RANGE (from lowest =MIN to highest =MAX) =
3. SUM (Total); (note that SUM = nm) =
4. AVG or Mean (m) =
5. Standard Deviation (s) or =STDEV =
Note: this may be sigma sub n-1 on some calculators
Formula, without calculator; s = SQRT (SumX^2 - (SumX)^2/n)/(n-1)
6. Standard error of the mean (SEm) = s/(SQRT n) =
7. Coefficient of Variation (CV) = s/m in % =
8. Mean Square (MS) = (s^2) or =VAR =
9. Variance = MS * (n-1)/n or =VARP on EXCEL spreadsheets =
Variance is a population parameter, MS is a sample statistic; Variance = sigma^2, MS = s^2
When comparing two samples, calculate:
10. Mean Difference (MD) = m1 - m2 =
11. Standard error of difference (SEd) = SQRT (SE1^2+SE2^2) =
12. t = MD/SEd (Use Fisher's t table to test significance) =
Complete the ANOV, using this method for Hand Calculator:
13. COUNT X (n) = total of both trees =
14. SUM X (for both trees) =
15. Cf = (SUM X)^2/n =
16. SUM X^2 (for both trees) =
17. SS Total = SUM X^2 - Cf =
18. SS Trees = ((Sum X1)^2 + (Sum X2)^2))/20 - Cf =
19. SS Error = SS Total - SS Trees =
Note: MS = SS/df; F = ratio of MS values, normally comparing (e.g.) MStrees with MS Error
Source df SS MS F
Total 39 79.775
Trees 1 27.225 27.23 19.69
Error 38 52.550 1.38
Spreadsheet approach:
SS TOTAL = 79.775 (=VARP(Range)*n)
SS TREES = 27.225 (=VARP(SUMs)*2/20)
HEET AND CALCULATOR
ith MS Error