WESTERN MINDANAO ADVENTIST ACADEMY

“The School for Better Future”

7028 San Pablo, Dumingag, Zamboanga del Sur

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Effect of Curcuma longa (Turmeric) Paste towards Incised Cutaneous Wound

Healing on Male Albino Mice

A Quantitative Research
Presented to
Senior High School Department of
Western Mindanao Adventist Academy
7028 San Pablo, Dumingag, Zamboanga del Sur

In Partial Fulfillment
Of the Requirements for the Subject
Practical Research II.

Zymbyr M. Frigillano
Shelly Van A. Mahusay
Rhyzmyrre Doreighn F. Romo

September 2019
 CHAPTER I

Introduction

This chapter presents an overview on the effect of Curcuma longa (Turmeric) paste

towards incised cutaneous wound healing on Male Albino Mice. This chapter bears The

problem and its background, Statement of the Problem, Hypothesis, Significance of the

Study, Scope and Delimitations, Definition of Terms, Conceptual Framework and

Research paradigm of the study.

The Problem and Its Background

Narrative reviews of Kaniwa (2013) and Pichler (2016) assert that some of the most

common adverse drug events (ADEs) present are the cutaneous drug reactions.

Medications such as anticoagulants, anti-rheumatoid drugs, and anti-inflammatory drugs

can cause or generate wounds by damaging skin integrity and impair wound healing

(Anderson, 2012). Drug therapy and medication such as penicillin, ampicillin, and

sulfonamides can cause skin damage and create wounds instead of actually healing it and

can even cause exanthems to patients (Bircher and Samuel, 2016).

Coffman (2013), Mohr Reyna and Amaya (2014), and Amaya (2015) support that

selected “natural” medications such as Curcuma longa (Turmeric) used topically can help

augment wound healing. Medicinal plants are considered very safe as there is no or

minimal side effects. These remedies are in sync with nature, making it an advantage of

using it as a herbal treatment of any age and gender. This is the reason why herbal treatment

is growing in popularity across the globe (Khan, 2016).

 Used internationally as an element of holistic medicine, Curcuma Longa

(Turmeric) has been providing the world with countless uses and immense health benefits

for thousands of years (Brandon, 2017). Curcumin, a natural yellow phenolic antioxidant

compound found on the plant Curcuma Longa (Turmeric), has been used for wound

healing. This plant has been involved in tissue remodeling, granulation tissue formation,

and collagen disposition that also helps increase fibroblast proliferation and vascular

density (Georgescu et. al., 2016).

Turmeric is a perennial plant that is cultivated throughout Southeast Asia, China,

Australia, and the South Pacific (Micromedex, Multum, and Kluwer, 2019). In the

southwestern part of India, it has been a staple of Ayurvedic medicine for more than 4,000

years and has been part of the Indian culture and cuisine of India, utilizing the root for

multiple applications. People in India became such devout believers in the root’s healing

and protective powers that they became planters and suppliers of this species (Brandon,

2017).

In Dumingag, Curcuma Longa (Turmeric) is present in the Municipality’s organic

garden, making products such as Turmeric powder available at the Organic Trading Post,

promoting its advantages in the field of health and medicine. Myrnalyn Baloro (2019), a

local on the said barangay, witnessed its wound healing effect by applying Curcuma Longa

(Turmeric) on the wound of her carabao.

Ninety-five percent of all lab animals are the laboratory mice and rats that play a

critical role in developing new medical wonders. These species reproduce quickly and have

a short lifespan of two to three years, so several generations of mice can be observed in a

relatively short period of time. Mice and rats are also relatively inexpensive and can be
bought in large quantities from commercial producers that breed rodents specifically for

research. The rodents are also generally mild-tempered and docile, making them easy for

researchers to handle, although some types of mice and rats can be more difficult to restrain

than others. Another reason that they are used as models in medical testing is that their

genetic, biological and behavior characteristics closely resemble those of humans, and

many symptoms of human conditions can be replicated in mice and rats (Melina, 2010).

The researchers made this study to know the possible outcome of Curcuma longa

(Turmeric) paste as an alternative medicine towards incised cutaneous wound on Male

Albino Mice.

Statement of the Problem

The general objective of this study is to find out the effect of Curcuma longa

(Turmeric) paste as an organic wound healing medicine towards Male Albino Mice. S.Y.

2019-2020. Specifically, it sought answers to the following questions:

1. What is the effect of Curcuma longa (Turmeric) paste applied to the incised cutaneous

wound of the Male Albino Mice with regards to: (a) wound size,

(b) weight?

1.1 Experimental group

1.2 Positive group

A. Treatment 1

B. Treatment 2
2. Is there an effect of Curcuma longa (Turmeric) paste as a healing medicine towards

Incised Cutaneous Wound on Male Albino Mice?

2.1 Experimental group

2.2 Positive group

A. Treatment 1

B. Treatment 2

Hypothesis

H0: There is no effect on the topical application of the Curcuma Longa (Turmeric)
towards incised cutaneous wound healing on Male Albino Mice.

Significance of the Study

The findings of this study will profoundly contribute the community considering

that Curcuma longa (Turmeric) paste has been attributed to be effective in terms of

incisional cutaneous wound healing. The study of Curcuma longa (Turmeric) paste

towards incised cutaneous wound healing on Male Albino Mice can be a learning paradigm

to the following:

To the students¸ the result of this study will give them knowledge and

understanding on Curcuma longa (Turmeric) in terms of healing open wounds.

To the teachers, this study will be a contribution as a teaching material to the

learning of the students in regards to the treatment of open wounds using Curcuma longa

(Turmeric).

To the school, this will aid the school to impart to the students the wound healing

benefits of Curcuma longa (Turmeric).

 To the community, the findings of this study will be of great help in surpassing

health related issues such as healing open wounds. This will let the community utilize this

medicinal plant as a home remedy.

To future researchers, this study will serve as a guide and as a reference material

for the future researchers who will be studying this study. This will provide them the

appropriate data that will aid as a foundation of learning and information.

Scope and Delimitations

The main focus of this study is to know the effect of Curcuma longa (Turmeric)

paste towards incised cutaneous wound healing on Male Albino Mice. The areas of the

study limit its effects on the wound size and weight of the Male Albino Mice that was

experimented.

The study did not use Curcuma longa (Turmeric) paste as an oral medicine, inhaler,

buccal medicine, injection, suppositories, drops, implants or soluble tablet to the Male

Albino Mice. The duration of the study limits to a 8-day observation. Any further results

exceeding the said days of the observation will not be considered as an additional data to

the research.
Definition of Terms

The following terms were operationally defined for clarity and better understanding

of the study:

Anticoagulant. The chemical substances that prevent or reduce coagulation of blood,

prolonging the clotting time.

Anti-inflammatory drugs. The drugs that help reduce inflammation and helps relieve

pain.

Anti-rheumatoid drugs. The drugs used in rheumatoid arthritis to slow down disease.

Albino Mice. A mice having congenital absence of pigment in the skin and hair (which are

white) and the eyes (which are usually pink).

Ayurvedic medicine. An ancient Indian system of medicine that uses a wide range of

treatments and techniques from medicinal plants such as Curcuma longa (Turmeric).

Bacitracin. Medication used to prevent minor skin infections caused by small cuts,

scrapes, or burns. Bacitracin is used in this study as a positive control group treatment

towards incised cutaneous wound healing on Male Albino Mice.

Control group. The control group in an experiment that uses a treatment that is known to

produce results but does not receive the test variable. Bacitracin and Fucidin is treated in

this group.

Curcuma longa (Turmeric). A flowering plant of the ginger family that is revered as a

sacred root for its incredible health benefits. It contains a yellow-colored chemical called

curcumin which is used as a herbal supplement, cosmetics ingredient, food flavoring, and

food coloring. Turmeric paste is used in this study as an experimental group medication

towards incisional cutaneous wound healing medicine on Male Albino Mice.

Cutaneous. Relating to, or affecting the skin. In this study, it affects the first skin layer of

the Male Albino Mice through incision.

Experimental group. The group in an experiment that receives the variable being tested.

In this study, this group is treated with Curcuma longa (Turmeric) paste.

Neomycin. A medication used for the treatment of primary and secondary skin infections

like wound cuts. Neomycin is used in this study as a positive control group treatment

towards incised cutaneous wound healing on Male Albino Mice.

Hair. A threadlike growth from the skin of a Male Albino Mice.

Incised. The act of marking a surface by cutting or carving. In this study, it means by

cutting the first skin layer of the Male Albino Mice using a hobby knife.

Laboratory Mice. The laboratory mouse is a small mammal of the order Rodentia which

is bred and used for scientific research. Laboratory mice are usually of the species Mus

musculus. This kind of mice is used as an experimental tool in this study.

Paste. A thick, soft moist substance produced by mixing Curcuma longa (Turmeric) with

water.

Wound. A type of injury which happens relatively quick in which skin is torn, cut, or

punctured. In this study, wound is introduced through a hobby knife.

Weight. A measurement that indicates how heavy the Male Albino Mice is.

Conceptual Framework

Figure 1 presents the conceptual framework that shows the concept structure of

the study. For the purpose of this study, three types of variables were used: (a) Dependent
variables which include the wound healing process on the wound size and weight of the

Male Albino Mice, (b) the independent variable which consists of Curcuma longa

(Turmeric) Paste, and (c) two positive control variables which are, Bacitracin and

Neomycin respectively.

Through the proposed framework, the effect of Curcuma longa (Turmeric) Paste

towards incised cutaneous wound healing on Male Albino Mice will be examined.

Furthermore, the proposed framework would provide an opportunity to determine

whether the Curcuma longa (Turmeric) has a significant effect towards incised cutaneous

wound size and weight on Male Albino Mice.

Research Paradigm

INDEPENDENT VARIABLE DEPENDENT VARIABLE

Curcuma longa (Turmeric) Paste Albino Mice (laboratory mice)

1. Wound healing process
a. Wound size
b. Weight
CONTROL VARIABLE

Bacitracin

Neomycin

Figure 1.