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DERPARTMENT OF EDUCATION
REGION IV-A CALABARZON
Division of Batangas
BALAYAN SENIOR HIGH SCHOOL
Balayan, Batangas

The Feasibility of Broom Weed


(Sida acuta Burm. f.) As an Anti-
Inflammatory
Ointment

Researchers:
Group 08
Maullon, John Ferdinand C.
Tong, Jayrex
Austria, Aira Rizz S.
Research Adviser:
Mrs. Ma. Rhoda E. Panganiban

Chapter 1
Introduction
Background of the Study

Science has drastically changed how the world works over the past years. These
changes lead to the discovery of scientists of different concepts of phenomena and lead
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to the creation and development of invention which became beneficial to humanity. It


became responsible for creation of new technological advances, exploration beyond
Earth’s boundary, the mechanisms of machines and engines, manipulation of genetics
and development in medicine.
In the past years, multiple researches about organic ointment such as Gumamela
(Hibiscus rosa-senensis Lin) and Ipil-ipil (Leucaena leucocephala) have been conducted
for an attempt to create an effective cure for wounds and inflammation. This researches
provide ideas in creating new products that will benefit the world for some common
problem humans are facing. Also, it encourages individuals to create new products from
common plants we see around that will surely help man for daily survival.
Broom weed (Sida acuta Burm. f.), commonly known as eskuba, uaualisan,
herbara, taking-baka is a specie of flowering plants that is believed to have originated in
Central America, it is now commonly found in tropical countries including Philippines
where it can be seen on vacant lots and forests. (Stuartxchange.org) Broom weeds
grow widely and rapidly over land areas since it is considered as an invasive plant.
Many individuals utilize this plant as a broom for cleaning mess on ground. Individuals
sundry these plants and flatten its branches to be able to use as a cleaning tool
However, anti-inflammatory ointments in the Philippines are usually costly. This
is due to the demand and expensiveness of chemicals and raw materials used in
making the products.
Creating an anti-inflammatory ointment from broom weed would greatly benefit
individuals. Moreover, this potential is a favorable instance whereas it would help to
introduce the other use of broom weed not only for cleaning and propagate its existence
to the community for greater use of it in the medical field. It is in the light that this
research study is to prove that broom weed has and anti-inflammatory property and can
be used as an ointment for treating inflammation and wounds effectively.
Statement of the Problem

The study entitled The Feasibility of Broom Weed (Sida acuta Burm. f.) as an
Anti-Inflammatory Ointment aims to use the properties of broom weed, a common pest
in vacant lots, in creating a medicinal treatment for inflammation and wound.

The study specifically aims to:

1. Perform the necessary process of ethanol extraction on broom weed


leaves to produce an anti-inflammatory ointment;
2. Produce an economic-friendly anti-inflammatory ointment; and
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3. Find if the amount of broom weed extract present in the ointment


affects its efficacy as an anti-inflammatory ointment.

Significance of the Study

For the Country, this research study aims to develop an economic-friendly anti-
inflammatory ointment for folks use. As we all know, anti-inflammatory ointments are
very costly here in our locality. Developing an economic-friendly ointment will surely
benefit countrymen. Consistency

For the Local Community, this research study will help the community lessen
the number of broom weed present in the locality by utilizing it in other purpose with
greater value than its traditional way of utilizing it.

For the Pharmaceutical Companies, this research study can give idea on the
potential of broom weed as use for medical purposes.

For the Students, this research study can provide more information about broom
weed and can be used for further study and development of broom weed.

For the Researchers, this study will provide information that can be use in
further studies of related topics.

Scope and Delimitation

The study focused on making an anti-inflammatory from broom weed to prove


that it has medicinal purpose rather than its tradition use.

For this research, a large number of broom weed leaves was used which are
gathered around the vicinity of Balayan Senior High School at Barangay Caloocan,
Balayan, Batangas.

The researchers conducted the study at Balayan Senior High School, from
November 2017 until February 2018. The study is conducted by Grade 12 STEM
students of Balayan Senior High School.

Hypothesis

Null Hypothesis
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The amount of broom weed extract will not affect the effectivity of the anti-
inflammatory ointment.

Alternative Hypotheses

The amount of broom weed extract will affect the effectivity of the anti-
inflammatory ointment.

Conceptual Framework
Figure 1 below illustrates the conceptual framework of the research. The
paradigm presents the variables that will be considered in the research.

Broom
Extraction Production Ointment
Weed
Figure 1: The Conceptual Framework of the Study

Chapter II
Review of the Related Literature

Broom Weed

According to StuartXchange.org, broom weed


(Sida acuta Burm.f.) is an erect, branched, slightly
hairy or nearly smooth, half-woody shrub growing to a
height of a meter or less. Leaves are lineolate, 3 to 5
centimeters long, with toothed margins, pointed tip
and blunt or rounded base. Flowers are yellow, about
1.3 centimeters in diameter, borne singly at the axils of
the leaves. Fruit is a capsule about 3.5 millimeters
long, with two projections and consists of 4 to 9
carpels enclosed by the calyx. Usually, it is found in
open places throughout the Philippines. Figure 2 Broom Weed

Chemical Composition of Broom Weed

Broom weed’s roots contain asparagine, a non-essential amino acid C 4H8N2O3


that is an amide of apparatus acid. Its leaves contain saponin, a chemical used in
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making soaps. The plant is considered abortifacient, anthelmintic, and antiemetic. Its
leaves are considered demulcent, diuretic, anthelmintic, vulnerary. Its roots considered
a bitter tonic, aphrodisiac, antipyretic, anti-rheumatic, demulcent, diaphoretic, stomachic
and vulnerary.

Usage of Broom Weed in Folklore Medicine

Broom weeds are commonly used in folklore medicines. Poultices made from
boiled leaves are applied to ulcers and sores. Decoction of roots and leaves are
emollient; taken internally for hemorrhoids, fever, and impotency. Its roots are use as
stomachic and antipyretic. An infusion with ginger added is given in intermittent fevers
and chronic bowel complaints, a teacupful twice a day. Root juice, sugared or mixed
with honey, used to expel worms. Fresh juice of roots applied to wounds and ulcers to
promote healing.

In other countries like in Nicaragua, the whole plant used for asthma, colds,
fever, worms, and renal inflammations. In India, used for fever, bronchitis, ulcers,
diarrhea, skin diseases; paste of leaves mixed with coconut oil used for dandruff and
hair strengthening. In Nigeria, used for malaria, ulcer, fever, breast cancer, poisoning,
inducing abortion. In Sri Lanka, roots and leaves used for hemorrhoids, fever,
impotency, gonorrhea, and rheumatism.  In India, seeds are given for enlarged glands
and for inflammatory. In Togo, West Africa, leaves used for eczema, kidney stones, and
headache. In Indian traditional medicine, used for treating liver disorders, urinary
disease and disorders of the blood and bile.

Anti-Inflammatory

Anti-inflammatory refers to the property of a substance or treatment that


reduces inflammation or swelling of a certain wound. Anti-inflammatory drugs make up
about half of analgesics, remedying pain by reducing inflammation as opposed
to opioids, which affect the central nervous system to block pain signaling to the brain.

Ointment

Ointments are preparations for external use, intended for application to the skin.
Typically, they have an oily or greasy consistency and can appear “stiff” as they are
applied to the skin. Ointments contain drug that may act on the skin or be absorbed
through the skin for systemic action. Many ointments are made from petroleum jelly.
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Like many other pharmaceutical preparations, they frequently contain preservatives and
may also contain aromatic substances and dyes to enhance patient acceptance

Beeswax

Beeswax is a natural wax produced in the bee hive of honey bees of the genus
Apis. It is mainly esters of fatty acids and various long chain alcohols. Typically, for a
honey beekeeper, 10 pounds of honey yields 1 pound of wax.

The wax is formed by worker bees that secrete it from eight wax-producing mirror
glands on the inner sides of the sternites (the ventral shield or plate of each segment of
the body) on abdominal segments 4 to 7. The sizes of these wax glands depend on the
age of the worker and after daily flights these glands begin to gradually atrophy.

The new wax scales are initially glass-clear and colorless (see illustration),
becoming opaque after mastication by the worker bee. The wax of honeycomb is nearly
white, but becomes progressively more yellow or brown by incorporation of pollen oils
and propolis. The wax scales are about 3 millimeters (0.12 in) across and 0.1
millimeters (0.0039 in) thick, and about 1100 are required to make a gram of wax.

Review of the Related Studies

A Study of the Anti-Inflammatory Effect of the Leaves of


Psidium Guajava Linn on Experimental Animal Models
Sarmistha Dutta and Swarnamoni Das Carrageenan

Induced paw edema is a biphasic response. The first phase was mediated
through the release of histamine, serotonin, and kinins and the second phase was due
to release of prostaglandin-like substances in 2–3 h. Drugs that inhibit carrageenan-
induced paw edema may act through inhibition of leukocyte migration and prostaglandin
synthesis. In the present study, maximum paw edema was observed at the end of 3 h of
carrageenan injection, that is, after the release of all these mediators of inflammation.
The probable cause of anti-inflammatory action against acute inflammation might be
due to the inhibition of some or all of the mediators released within 3 h of carrageenan
injection. Subacute inflammation involves infiltration of macrophages, neutrophils, and
proliferation of fibroblasts. Flavonoids found in the plant extracts have antiproliferative
activity, which is found to cause a decrease in the weight and volume of contents of
granuloma. Also cytokines are found to be the important mediators in the formation and
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maintenance of granuloma. So it is speculated that the anti-inflammatory activity against


subacute inflammation by PGE may be due to flavonoids found in the extract. Freund’s
adjuvant-induced arthritis is a widely used chronic model for inflammation. One of the
reasons for the wide utilization of this model is due to the strong correlation between the
efficacy of therapeutic agents in this model and in rheumatoid arthritis in humans and it
is characterized by very rapid erosive disease. Adjuvant arthritis is characterized by
weight loss and body weight loss is associated with increased production of
proinflammatory cytokines, such as tumor necrosis growth factor-α (TNF-α) and
interleukin-1 (IL-1).

These cytokines have profound effects on the hormones that govern metabolism
and also act directly on the metabolic target organs, such as muscle, liver, gut, and
brain. The result is an increase in resting energy expenditure, a net export of amino
acids from muscle to liver, an increase in gluconeogenesis and a marked shift in liver
protein synthesis away from albumin and toward production of acute phase proteins,
such as fibrinogen and C-reactive protein. Thus the arthritis-induced reduction in weight
can be prevented by the test drug and it may be due to inhibition of TNF-α and IL-1. The
leaves of guava are rich in flavonoids, in particular, quercetin. Much of guava’s
therapeutic activity is attributed to these flavonoids. The flavonoids have demonstrated
anti-inflammatory activity. Also flavonoids have antiproliferative activity, which is found
to cause a decrease in the weight and volume of contents of granuloma in inflammation

Synthesis: The present study demonstrated that the ethanolic extract of the leaves of
PGE showed significant anti-inflammatory activity against acute, sub-acute,
and chronic inflammation. However, further studies are required to
establish and elaborate the molecular mechanism for proper clinical utility.
Also, the development of more purified products of PGE for the treatment
of various inflammatory diseases should be encouraged.

Boswellia Serrata, A Potential Antiinflammatory Agent: An Overview

M. Z. Siddiqui

Gum-resin extract of Boswellia Serrata have been traditionally used in folk


medicine for centuries to treat various chronic inflammatory diseases according to the
study conducted by M.Z Siddiqui.
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The resin of Boswellia Serrata (Salai) has been use as an incense in religious
and cultural ceremonies and also in medicine since time immemorial wherein, the resin
is stored in specially made basket for oil content removal as well as getting the resin
solidified and graded according to its flavor, color, shape and size after the process.

Furthermore, Boswellia Serrata can usually found in dry mountainous region of


India, Northern Africa and Middle East and despite its historical, religious, cultural and
medicinal importance, Boswellia has not been thoroughly studied, and gaps still exist
between our knowledge of the traditional uses of the resin and the scientific data
available.

Synthesis: The study of Boswellia Serrata as an potential Anti-Inflammatory is a proof


that there are still large number of plants and trees that has been
traditionally used as an anti-inflammatory agent without much scientific
knowledge or basis although it serve as an alternative replacement for the
generic brand for inflammation. According the statements above, It is
therefore concluded that the feasibility of broom weed (Sida acuta Burm.f.)
as an anti-inflammatory ointment has a great potential as a treatment for
inflammation if it would be based on the study of Bosswelia Serrata.

The Wound Healing Activity of Sida Acuta in Rats

M. Z. Siddiqui

Effects of topical administration of methanolic extract of Sida acuta ointment was


studied respectively on two types of wound models in rats, (i) the excision and (ii) the
incision wound model. The ointment of the methanol extract of Sida acuta produced
significant response in both of the wound types tested. In the excision model the
extract treated wounds were found to epithelialise faster and the rate of wound
contraction was higher, as compared to control wounds. The extract facilitates the
healing process as evidenced by increase in the tensile strength in the incision model.
The results were also comparable to those of a standard drug nitrofurazone.

Synthesis: The study entitled “The wound healing activity of Sida Acuta in rats” has
been proved that the plant Broom Weed (Sida Acuta) contains a healing
property that helps to expedite the recuperation of a certain wound to an
experimental rat. Moreover, the study of wound healing activity of Sida
Acuta in rats may be related to the “Feasibility of Broom Weed (Sida Acuta)
as an anti-inflammatory ointment since both study intends to heal a specific
wound by using the same raw material.
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Effects of Brassica Oleracea in Rats Skin Wound Healing

Aline Rebolla et. al

Twenty four rats undergone biometric analysis in order to examine the changes
in the condition in their wound healing skin. Subjects were divided to 4 different groups,
whereas ( C ) untreated animals and ( T ) treated animals, which has subdivision of two
experimental time scales ( 7 and 16 days ). 3cm2 skin wound was made and 100ml of
Brassica Oleracea was applied twice a day. In addition, the wound area that have been
taken also undergone histological and histhomorphometric analysis for calculation the
amount of birefringent collagen fibers content within the skin. In the result, it was found
out that there is no significant difference between groups in both experimental times
studied. However, it also showed that the number of type I collagen fibers was
significantly higher in the specimen of group T16, which also implies that B. Oleracea is
an accelerant of the process in the healing of wounded skin by boosting the number of
type I collagen fibers and newly formed tissue.

Synthesis: Before the application of ointments on wounded skin of rats, the affected
area must cleaned necessarily. Usage of aesthetics and aspirins within rats
can also be part of the considerations. This study shows that Brassica
Oleracea has significant impact to the healing process of wounded skin in
rats where which it speed up the healing process by increasing the amount
of collagen content within the skin. Relative to the study of the researchers,
they concluded that the longer the application of the ointments in wounded
skin, the better the result would be.

Chapter III
Methodology
To produce an anti-inflammatory ointment from broom weed (Sida acuta Burm.
f.), the researchers will conduct an experiment and the following apparatus are used;
Scissors for cutting leaves of broom weed, reagent bottles and plastic containers for
storage, mortar and pestle for pounding the leaves of broom weed, heating pad and
beakers for boiling, bees wax, triple beam balance for weighting broom weed leaves,
ethyl alcohol, cheese cloth and strainer for filtering, and seven (7) white mice of
analogous size, weight and age.

To produce an anti-inflammatory ointment, the following procedure is performed:


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1. Gathering of broom weed


20.72 kg of broom weed leaves is gathered around the vicinity of Balayan
Senior High School, Brgy. Caloocan, Balayan, Batangas.
2. Preparation of materials
Scissors, plastic containers, mortar and pestle, alcohol lamp and beakers,
bees wax, triple beam balance, measuring spoons and cups, ethyl alcohol,
cheese cloth, and seven (7) mice of analogous size, weight and age are
prepare before conducting the experiment. All necessary materials and
apparatus are washed and sterilized first to ensure the accurateness of
the results.
3. Preparation of broom weed
a) Gathering of broom weed leaves from stems
The leaves from the newly gathered broom weed are removed from its
stem thru manual sorting to separate the leaves from its seeds and stems.
b) Washing of broom weed leaves
The newly gathered broom weed leaves are washed in running tap water
to remove unwanted materials on it.
c) Sun Drying
The leaves are sun dried to remove its water content for easy pounding
and extraction process.

4. Pounding of broom weed leaves


Using a mortar and pestle, the broom weed leaves are pounded to better get the
extract of the broom weed leaves.
5. Preparation of the beeswax
The beeswax are melted in order to mix the broom weed extract after extraction.
6. Making the ointment proper
a) Boiling
The pounded broom weed leaves are boiled in an ethyl alcohol with the
ratio of 34.5625: 500 ml until only the broom weed extract remain in the
beakers.
b) Filtering
The remained extract in the beaker are filtered using cheese cloth to
remove bits broom weed leaves in the extract.
c) Ethanol washing
To ensure that pure broom weed extract is used in the study, the extract
are boiled again in an ethyl alcohol.
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d) Mixing extract with beeswax


The filtered broom weed leaves extract are mixed in the heated beeswax
to obtain the ointment texture and comfort.
Different treatments with different concentration of extract will be prepare.

Table 1: Different amount of broom weed extract mixed with beeswax


Amount of Beeswax Amount of Broom Weed
Treatment
(g) Extract (ml)
a 10 10
b 10 7.5
c 10 5
d 10 2.5
e 10 None
f None 10
G (Control) None None

7. Experimentation
a) Seven (7) white mice are used in the experiment to measure the effect of
broom weed as an anti-inflammatory ointment.
 The researchers will perform seven (7) treatments to seven (7) white
mice.
 Each white mice is cut 4 cm on their back each.
 The researchers will apply the ointment in wounds of mice twice a day,
every 7 am and 4 pm and will be observe for 10 consecutive days.
 The observation will be recorded in the table below:

Table 2: Shows the wound recovery on each mice per day for 10 days

Treatment Wound Recovery in Day


Set Up
Applied 0 1 2 3 4 5 6 7 8 9 10

A a 4
B b 4
C c 4
D d 4
E e 4
F f 4
G g 4
Note: %wound recovery = |(size of wound Day 0-size of wound Day N)/4 cm x 100|
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Chapter IV
Results and Discussions
This chapter discusses the results and analysis of the study from the data
collected in the process of conducting the study. This chapter also includes the initial
findings of the study.

Findings

Upon conducting the study, the following results were gathered:

1. The researchers are able to perform ethanol extraction in order to have


broom weed extract which are used in making the anti-inflammatory
ointment. In performing the extraction, the researchers kept the temperature
not higher than 80 C. Since alcohol is a volatile material which easily
evaporates when expose to high temperature, maintaining a certain
temperature will make the extraction more effective in gathering the extract.
With the ratio of 34.5625 g of pounded broom weed leaves is 500 ml of ethyl
alcohol, the researchers successful gathered extract from the broom weed
leaves which is necessary in performing this research study. The ethanol
extraction performed by the researchers has two parts. First is the initial
extractions which took almost 45 minutes followed by the ethanol washing
which took almost one hour in order to gather necessary extract for the
study. The gathered extract were then used to create the ointment by binding
it with the melted beeswax in order to attain the ointment texture and
comfort.

2. In the process of making an anti-inflammatory ointment, the following


expenses were enumerated in order to evaluate how much a single ointment
costs on its production.

Table 3: Expenses in Making the Anti-Inflammatory Ointment per Ointment

Amount/Quantity
Material Price per Unit Total Price
Used
Beeswax 10 g PHP 250.00 per kg PHP 2.50
Ethyl Alcohol 250 ml PHP 65.00 per 500 ml PHP 32.50
Container 1 PHP 13.00 per container PHP 13.00
Total PHP 48.00
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In the process of making the anti-inflammatory ointment, the


researchers spent PHP 48.00 on producing 20 g of ointment which is
economically lower than the prices of anti-inflammatory ointments in the local
market which usually cost PHP 75.00 and above. This shows that the
produced anti-inflammatory ointment’s price is 36% lower than the
commercially made ones.

3. To find the efficacy of the anti-inflammatory ointment, the researchers would


be needing enough time in order to make observations to better prove the
effectiveness of the broom weed as an anti-inflammatory ointment. After
gathering the necessary data, the researchers will immediately proceed in
the statistical test to identify if the amount of broom weed extract present in
the ointment affects its efficacy as an anti-inflammatory ointment.
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Chapter V
Summary of Findings, Conclusions and Recommendations
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Bibliography
www.ncbi.nlm.nih.gov/pmc/articles/PMC3093039
www.ncbi.nlm.nih.gov/pmc/articles/PMC3093039
www.stuartxchange.org/ualisualisan.html
www.researchgate.net/publication/286733806
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Chapter IV
Results and Discussion

A. Analysis

Amount of
Amount of
Treatment sodium Obtain Fiber
cogon
Hydroxide
A 50 g 10 g None
B 50 g 20 g 8g
C 50 g 30 g 10 g
D 50 g 0g none
Table 1

Table 1 shows the amount of obtain fiber from different amount of


sodium hydroxide solution and it also shows it strength.

Forced Applied (N)


Treatment Obtain Fiber
5N 8N 10 N
A None X X X
B 8g / / X
C 10 g / / /
D None X X X

TABLE 1.1
Table 1.1 shows the strength of the obtain product

Statistical Analysis

No. of No. of
Treatment Observed Expected O-E (O-E)² (O-E)²/E
(O) (E)
A (10) 0 50 -50 2500 50
B (20) 8 50 -40 1600 32
C (30) 10 50 -42 1764 35.28
D (0) 0 0 0 0 0
60 18 150 -132 5864 117.28
Table 1.2

Table 1.2 shows the statistical data used for the computation using chi
square.

STATISTICAL TEST: CHI SQUARE


Xc= 117.28
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Df= (C-1) (R-1)

Df= 6

Xt= 5.35

Reject Ho if X- calculate > X tabular.

Therefore, Ho is rejected. There is a significant relationship on the amount


of solution and the obtain thread.
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Chapter V
Conclusion

In the progression of the study, the researchers were able to collect


and gathered all the required data. These were possible by understanding
and analyzing the information we gathered from the start. The following
conclusions were produced after the study:

a) Cogon grass can produce thread, therefore it can use in the production
of textile.
b) The solution used affects the strength of the produce product, Using
sodium hydroxide solution is better than using detergent.
c) The amount of sodium hydroxide solution has something to do with
the strength of the produce thread. The more sodium hydroxide
solution the better for the fiber.
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Recommendation

Based on the result and conclusion of the researchers, the following


recommendations offer:

1. Larger amount of cogon grass should use for larger production.


2. Make use of another product using this materials.

3. Try to vary on the amount of water use if the same study will conduct.

4. Make another process to obtain thread.

5. Use appropriate apparatus for the study.


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Bibliography
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Appendix

Criteria of Rejection

(No. of observed – No of Expected)2/ Expected

A)

(0-50)2/50 = 50

B)

(8-50)2/ 50 = 32

C)

(10-50)2/50=35.28

D)

(0-0)2/50= 0

∑ABCD

Xc = 117.28

Df= (C-1) (R-1)

Df= 6

Xt= 5.35

Reject Ho if X- calculate > X tabular


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Flow Chart
Production of Lagerstroemia speciosa (Banaba Leaves) Ointment
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Flow Chart
The Feasibility of Broom Weed (Sida acuta Burm. f.) As an Anti-Inflammatory Ointment

Gathering of Materials

Collection of Broom Weed Leaves

Cutting of Broom Weed Leaves From its Stem

Washing of Broom Weed Leaves

Sun-drying of Broom Weed Leaves

Pounding of Dried Broom Weed Leaves

Production of Ointment

Melting of Beeswax
Measuring of Broom Weed Leaves
Filtering of Beeswax
Ethanol Extraction
Cooling and Storage
Initial Filtration

Ethanol Washing

Second Filtration

Cooling and Storage

Binding of Broom Weed Ethanol Extract and Beeswax


`
Measuring and Weighing of Broom Weed Ethanol Extract and Beeswax

Melting of Beeswax

Mixing of Banaba Ethanol Extract and Beeswax

Cooling and Storage

Testing of Broom Weed Ointment

Feasibility Test using varying amounts

Treatment A

Treatment B

Treatment C

Treatment D
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Binding of of Broom Weed Ethanol Extract and Beeswax

Measuring and Weighing of Broom Weed Ethanol Extract and Beeswax

Melting of Beeswax

Mixing of Banaba Ethanol Extract and Beeswax

Cooling and Storage

Testing of Broom Weed Ointment

Feasibility Test using varying amounts

Treatment A

Treatment B

Treatment C

Treatment D

Treatment E

Treatment F

Treatment G
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Pictures

Gathering of Cogon Grass

Washing of Cogon Grass


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Pressing of Cogon Grass

Boiling of Cogon Grass


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Adding of Sodium Hydroxide in the boiling water

C B A

Boiling of the Cogon Grass Showing the three diffirent treatments


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Sorting of Cogon Grass

Produced Thread
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Twisting of the produce thread

Manual Weaving of the Produce thread


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Testing the strength of the fabric


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Curriculum Vitae
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Name: John Ferdinand C. Maullon


Address: 068 Brgy. Sucol, Balayan, Batangas
Birthdate: October 28, 1999
Contact Number: 09066940657
Email: ferdiemaullon@gmail.com
Parents
Father: Fernando P. Maullon
Mother: Estelita C. Maullon
Educational Background
Pre Elementary: Poinsettia Daycare Center
Elementary: Sucol Elementary School
High School: Balayan National High School

Name: Angelica Baylosis


Address: Duhatan, Balayan, Batangas
Birthdate: January 05, 2000
Contact Number: 09754727243
Email: angelicabaylosis0500@yahoo.com
Parents
Father: Romeo Baylosis
Mother: Felipa B. Maglunog
Educational Background
Pre Elementary: Duhatan Elementary School
Elementary: Duhatan Elementary School
High School: Balayan National High School
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Name: Maria Kimberly C. Ellao


Address: Lumbangan, Tuy, Batangas
Birthdate: October 12, 1999
Contact Number: 09972072312
Email: ellaomariakimberly@gamail.com
Parents
Father: Apolinar Ellao
Mother: Efrene C. Ellao
Educational Background
Pre Elementary: N/A
Elementary: Balayan East Central School
High School: Balayan National High School

Name: Maribeth B. Mayuga


Address: 296 Nangkaan Brgy. Magabe,
Balayan, Batangas
Birthdate: February 04, 1999
Contact Number: +63 935 552 9767
Email: mayugabeth@yahoo.com
Parents
Father: Conrado R. Mayuga
Mother: Elna B. Mayuga
Educational Background
Pre Elementary: Birds of Paradise Day Care Center
Elementary: Jose Chua Ben Chong Elementary School
High School: Balayan National High School

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