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DERPARTMENT OF EDUCATION
REGION IV-A CALABARZON
Division of Batangas
BALAYAN SENIOR HIGH SCHOOL
Balayan, Batangas
Researchers:
Group 08
Maullon, John Ferdinand C.
Tong, Jayrex
Austria, Aira Rizz S.
Research Adviser:
Mrs. Ma. Rhoda E. Panganiban
Chapter 1
Introduction
Background of the Study
Science has drastically changed how the world works over the past years. These
changes lead to the discovery of scientists of different concepts of phenomena and lead
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The study entitled The Feasibility of Broom Weed (Sida acuta Burm. f.) as an
Anti-Inflammatory Ointment aims to use the properties of broom weed, a common pest
in vacant lots, in creating a medicinal treatment for inflammation and wound.
For the Country, this research study aims to develop an economic-friendly anti-
inflammatory ointment for folks use. As we all know, anti-inflammatory ointments are
very costly here in our locality. Developing an economic-friendly ointment will surely
benefit countrymen. Consistency
For the Local Community, this research study will help the community lessen
the number of broom weed present in the locality by utilizing it in other purpose with
greater value than its traditional way of utilizing it.
For the Pharmaceutical Companies, this research study can give idea on the
potential of broom weed as use for medical purposes.
For the Students, this research study can provide more information about broom
weed and can be used for further study and development of broom weed.
For the Researchers, this study will provide information that can be use in
further studies of related topics.
For this research, a large number of broom weed leaves was used which are
gathered around the vicinity of Balayan Senior High School at Barangay Caloocan,
Balayan, Batangas.
The researchers conducted the study at Balayan Senior High School, from
November 2017 until February 2018. The study is conducted by Grade 12 STEM
students of Balayan Senior High School.
Hypothesis
Null Hypothesis
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The amount of broom weed extract will not affect the effectivity of the anti-
inflammatory ointment.
Alternative Hypotheses
The amount of broom weed extract will affect the effectivity of the anti-
inflammatory ointment.
Conceptual Framework
Figure 1 below illustrates the conceptual framework of the research. The
paradigm presents the variables that will be considered in the research.
Broom
Extraction Production Ointment
Weed
Figure 1: The Conceptual Framework of the Study
Chapter II
Review of the Related Literature
Broom Weed
making soaps. The plant is considered abortifacient, anthelmintic, and antiemetic. Its
leaves are considered demulcent, diuretic, anthelmintic, vulnerary. Its roots considered
a bitter tonic, aphrodisiac, antipyretic, anti-rheumatic, demulcent, diaphoretic, stomachic
and vulnerary.
Broom weeds are commonly used in folklore medicines. Poultices made from
boiled leaves are applied to ulcers and sores. Decoction of roots and leaves are
emollient; taken internally for hemorrhoids, fever, and impotency. Its roots are use as
stomachic and antipyretic. An infusion with ginger added is given in intermittent fevers
and chronic bowel complaints, a teacupful twice a day. Root juice, sugared or mixed
with honey, used to expel worms. Fresh juice of roots applied to wounds and ulcers to
promote healing.
In other countries like in Nicaragua, the whole plant used for asthma, colds,
fever, worms, and renal inflammations. In India, used for fever, bronchitis, ulcers,
diarrhea, skin diseases; paste of leaves mixed with coconut oil used for dandruff and
hair strengthening. In Nigeria, used for malaria, ulcer, fever, breast cancer, poisoning,
inducing abortion. In Sri Lanka, roots and leaves used for hemorrhoids, fever,
impotency, gonorrhea, and rheumatism. In India, seeds are given for enlarged glands
and for inflammatory. In Togo, West Africa, leaves used for eczema, kidney stones, and
headache. In Indian traditional medicine, used for treating liver disorders, urinary
disease and disorders of the blood and bile.
Anti-Inflammatory
Ointment
Ointments are preparations for external use, intended for application to the skin.
Typically, they have an oily or greasy consistency and can appear “stiff” as they are
applied to the skin. Ointments contain drug that may act on the skin or be absorbed
through the skin for systemic action. Many ointments are made from petroleum jelly.
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Like many other pharmaceutical preparations, they frequently contain preservatives and
may also contain aromatic substances and dyes to enhance patient acceptance
Beeswax
Beeswax is a natural wax produced in the bee hive of honey bees of the genus
Apis. It is mainly esters of fatty acids and various long chain alcohols. Typically, for a
honey beekeeper, 10 pounds of honey yields 1 pound of wax.
The wax is formed by worker bees that secrete it from eight wax-producing mirror
glands on the inner sides of the sternites (the ventral shield or plate of each segment of
the body) on abdominal segments 4 to 7. The sizes of these wax glands depend on the
age of the worker and after daily flights these glands begin to gradually atrophy.
The new wax scales are initially glass-clear and colorless (see illustration),
becoming opaque after mastication by the worker bee. The wax of honeycomb is nearly
white, but becomes progressively more yellow or brown by incorporation of pollen oils
and propolis. The wax scales are about 3 millimeters (0.12 in) across and 0.1
millimeters (0.0039 in) thick, and about 1100 are required to make a gram of wax.
Induced paw edema is a biphasic response. The first phase was mediated
through the release of histamine, serotonin, and kinins and the second phase was due
to release of prostaglandin-like substances in 2–3 h. Drugs that inhibit carrageenan-
induced paw edema may act through inhibition of leukocyte migration and prostaglandin
synthesis. In the present study, maximum paw edema was observed at the end of 3 h of
carrageenan injection, that is, after the release of all these mediators of inflammation.
The probable cause of anti-inflammatory action against acute inflammation might be
due to the inhibition of some or all of the mediators released within 3 h of carrageenan
injection. Subacute inflammation involves infiltration of macrophages, neutrophils, and
proliferation of fibroblasts. Flavonoids found in the plant extracts have antiproliferative
activity, which is found to cause a decrease in the weight and volume of contents of
granuloma. Also cytokines are found to be the important mediators in the formation and
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These cytokines have profound effects on the hormones that govern metabolism
and also act directly on the metabolic target organs, such as muscle, liver, gut, and
brain. The result is an increase in resting energy expenditure, a net export of amino
acids from muscle to liver, an increase in gluconeogenesis and a marked shift in liver
protein synthesis away from albumin and toward production of acute phase proteins,
such as fibrinogen and C-reactive protein. Thus the arthritis-induced reduction in weight
can be prevented by the test drug and it may be due to inhibition of TNF-α and IL-1. The
leaves of guava are rich in flavonoids, in particular, quercetin. Much of guava’s
therapeutic activity is attributed to these flavonoids. The flavonoids have demonstrated
anti-inflammatory activity. Also flavonoids have antiproliferative activity, which is found
to cause a decrease in the weight and volume of contents of granuloma in inflammation
Synthesis: The present study demonstrated that the ethanolic extract of the leaves of
PGE showed significant anti-inflammatory activity against acute, sub-acute,
and chronic inflammation. However, further studies are required to
establish and elaborate the molecular mechanism for proper clinical utility.
Also, the development of more purified products of PGE for the treatment
of various inflammatory diseases should be encouraged.
M. Z. Siddiqui
The resin of Boswellia Serrata (Salai) has been use as an incense in religious
and cultural ceremonies and also in medicine since time immemorial wherein, the resin
is stored in specially made basket for oil content removal as well as getting the resin
solidified and graded according to its flavor, color, shape and size after the process.
M. Z. Siddiqui
Synthesis: The study entitled “The wound healing activity of Sida Acuta in rats” has
been proved that the plant Broom Weed (Sida Acuta) contains a healing
property that helps to expedite the recuperation of a certain wound to an
experimental rat. Moreover, the study of wound healing activity of Sida
Acuta in rats may be related to the “Feasibility of Broom Weed (Sida Acuta)
as an anti-inflammatory ointment since both study intends to heal a specific
wound by using the same raw material.
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Twenty four rats undergone biometric analysis in order to examine the changes
in the condition in their wound healing skin. Subjects were divided to 4 different groups,
whereas ( C ) untreated animals and ( T ) treated animals, which has subdivision of two
experimental time scales ( 7 and 16 days ). 3cm2 skin wound was made and 100ml of
Brassica Oleracea was applied twice a day. In addition, the wound area that have been
taken also undergone histological and histhomorphometric analysis for calculation the
amount of birefringent collagen fibers content within the skin. In the result, it was found
out that there is no significant difference between groups in both experimental times
studied. However, it also showed that the number of type I collagen fibers was
significantly higher in the specimen of group T16, which also implies that B. Oleracea is
an accelerant of the process in the healing of wounded skin by boosting the number of
type I collagen fibers and newly formed tissue.
Synthesis: Before the application of ointments on wounded skin of rats, the affected
area must cleaned necessarily. Usage of aesthetics and aspirins within rats
can also be part of the considerations. This study shows that Brassica
Oleracea has significant impact to the healing process of wounded skin in
rats where which it speed up the healing process by increasing the amount
of collagen content within the skin. Relative to the study of the researchers,
they concluded that the longer the application of the ointments in wounded
skin, the better the result would be.
Chapter III
Methodology
To produce an anti-inflammatory ointment from broom weed (Sida acuta Burm.
f.), the researchers will conduct an experiment and the following apparatus are used;
Scissors for cutting leaves of broom weed, reagent bottles and plastic containers for
storage, mortar and pestle for pounding the leaves of broom weed, heating pad and
beakers for boiling, bees wax, triple beam balance for weighting broom weed leaves,
ethyl alcohol, cheese cloth and strainer for filtering, and seven (7) white mice of
analogous size, weight and age.
7. Experimentation
a) Seven (7) white mice are used in the experiment to measure the effect of
broom weed as an anti-inflammatory ointment.
The researchers will perform seven (7) treatments to seven (7) white
mice.
Each white mice is cut 4 cm on their back each.
The researchers will apply the ointment in wounds of mice twice a day,
every 7 am and 4 pm and will be observe for 10 consecutive days.
The observation will be recorded in the table below:
Table 2: Shows the wound recovery on each mice per day for 10 days
A a 4
B b 4
C c 4
D d 4
E e 4
F f 4
G g 4
Note: %wound recovery = |(size of wound Day 0-size of wound Day N)/4 cm x 100|
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Chapter IV
Results and Discussions
This chapter discusses the results and analysis of the study from the data
collected in the process of conducting the study. This chapter also includes the initial
findings of the study.
Findings
Amount/Quantity
Material Price per Unit Total Price
Used
Beeswax 10 g PHP 250.00 per kg PHP 2.50
Ethyl Alcohol 250 ml PHP 65.00 per 500 ml PHP 32.50
Container 1 PHP 13.00 per container PHP 13.00
Total PHP 48.00
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Chapter V
Summary of Findings, Conclusions and Recommendations
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Bibliography
www.ncbi.nlm.nih.gov/pmc/articles/PMC3093039
www.ncbi.nlm.nih.gov/pmc/articles/PMC3093039
www.stuartxchange.org/ualisualisan.html
www.researchgate.net/publication/286733806
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Chapter IV
Results and Discussion
A. Analysis
Amount of
Amount of
Treatment sodium Obtain Fiber
cogon
Hydroxide
A 50 g 10 g None
B 50 g 20 g 8g
C 50 g 30 g 10 g
D 50 g 0g none
Table 1
TABLE 1.1
Table 1.1 shows the strength of the obtain product
Statistical Analysis
No. of No. of
Treatment Observed Expected O-E (O-E)² (O-E)²/E
(O) (E)
A (10) 0 50 -50 2500 50
B (20) 8 50 -40 1600 32
C (30) 10 50 -42 1764 35.28
D (0) 0 0 0 0 0
60 18 150 -132 5864 117.28
Table 1.2
Table 1.2 shows the statistical data used for the computation using chi
square.
Df= 6
Xt= 5.35
Chapter V
Conclusion
a) Cogon grass can produce thread, therefore it can use in the production
of textile.
b) The solution used affects the strength of the produce product, Using
sodium hydroxide solution is better than using detergent.
c) The amount of sodium hydroxide solution has something to do with
the strength of the produce thread. The more sodium hydroxide
solution the better for the fiber.
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Recommendation
3. Try to vary on the amount of water use if the same study will conduct.
Bibliography
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Appendix
Criteria of Rejection
A)
(0-50)2/50 = 50
B)
(8-50)2/ 50 = 32
C)
(10-50)2/50=35.28
D)
(0-0)2/50= 0
∑ABCD
Xc = 117.28
Df= 6
Xt= 5.35
Flow Chart
Production of Lagerstroemia speciosa (Banaba Leaves) Ointment
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Flow Chart
The Feasibility of Broom Weed (Sida acuta Burm. f.) As an Anti-Inflammatory Ointment
Gathering of Materials
Production of Ointment
Melting of Beeswax
Measuring of Broom Weed Leaves
Filtering of Beeswax
Ethanol Extraction
Cooling and Storage
Initial Filtration
Ethanol Washing
Second Filtration
Melting of Beeswax
Treatment A
Treatment B
Treatment C
Treatment D
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Melting of Beeswax
Treatment A
Treatment B
Treatment C
Treatment D
Treatment E
Treatment F
Treatment G
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Pictures
C B A
Produced Thread
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Curriculum Vitae
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