Wound Healing Effect of Mallungay Moringa oliefera Leaf Extract on the Wounds

of Mice Mus musculue

Edrian C. Villodres

A RESEARCH PLAN

Submitted as an Output in Research 9

Secondary Level

Physical Science

Antique National School

Mrs. Kathleen Pearl Joy P. Llera

Research Adviser

May 2023
 Wound Healing Effect of Mallungay Moringa oliefera Leaf Extract on the Wounds
of Mice Mus musculus
A. Rationale
The healing process of wounds has been a significant concern for various living things,

especially with the risks of infection and scarring. The use of natural products has been a

common method of promoting wound healing due to their potential anti-inflammatory and

antimicrobial properties (ncbi.nlm.nih.gov, 2018). Mallungay, scientifically known as Moringa

oliefera, is a plant commonly found in tropical countries, containing several active compounds

with diverse medicinal properties, including wound healing (Farooq Anwar, 2006).

The use of natural products such as Moringa oliefera leaf extract has increased interest

among researchers and health professionals due to their potential wound-healing properties. If

this study provides evidence in support of the healing effect of Moringa oliefera leaf extract on

mice, it can open up new possibilities for the plant's use as a therapeutic agent (Jed W. Fahey,

2005). This study emphasizes the need to continue to explore natural and accessible

alternatives to wound healing.

Several studies have shown the wound-healing properties of Moringa oliefera leaf

extract. The plant contains flavonoids and phenolic acids, which are potent antioxidants that

speed up the healing process (Simona Martinotti, 2015). However, while previous research has

been conducted to evaluate the effects of extracts of the leaves on rats, there is a paucity of

research on the healing effect of Moringa oliefera leaf extract on mice. Mice models provide a

suitable alternative for evaluating the effects of various substances on the healing process

because of their genetic similarities with humans (Biol, 2022).

In conclusion, this study aims to investigate the potential healing effects of Moringa

oliefera leaf extract on wounds in a mouse model. The use of the extract provides a promising

solution for wound healing, thus reducing the need for expensive conventional treatments that
may cause adverse reactions. The study will examine the changes in skin structure, wound

contraction, and re-epithelialization rate over a given healing period. The findings of this

investigation may provide additional information on the safety and efficacy of the extract,

which can make it more accessible to mice and also possible for humans with minor injuries.

A..Questions, Hypothesis, Engineering Goals, Expected Outcomes

1. Objectives of the Study

The study aims to find out if Malunggay Moringa oliefera leaf extract have a

wound healing effect on wounds of Mice Mus musculus.

Specifically, the study will aim to:

1.1 determine the wound healing effect of Malunggay Moringa oleifera leaf

extract on the wounds of Mice Mus musculus at different concentrations.

1.2 determine if the wound healing effect of Malunggay Moringa oleifera leaf

extract is effective like the commercial medicine for wound healing.

2. Hypothesis of the Study

The following hypothesis will be put forward:

2.1 there is no significant difference in the effectivity time of the wound healing

effect of Malunggay Moringa oleifera leaf extract on the wounds of Mice

Mus musculus at different concentrations.

2.2 there is no significant difference in the effectivity time between the wound

healing effect of Malunggay Moringa oleifera leaf extract and commercial

medicine for wound healing.

3. Expected Outcomes
 The study will expect that:

3.1 the Malunggay Moringa oliefera leaf extract will have a wound healing effect

on wounds of Mice Mus musculus at different concentrations.

3.2 the wound healing effect of Malunggay Moringa oleifera leaf extract is

effective like the commercial medicine for wound healing.

C..Procedures

1. Preparation of Malunggay Moringa Oleifera leaf extract.

Fresh Malunggay Moringa Oleifera leaves will be collected from Atabay, Tobias Fornier,

Antique and will wash them thoroughly with distilled water. Then, will also dry the leaves and

grind them into a fine powder. Weigh 100g of the powdered leaves and extract them using 500

ml of distilled water. Boil the mixture for 10 minutes, then cool and filter it using a sterile filter

paper. Store the extract in a sterilized container at 4°C until needed.

2. Animal selection and preparation.

Use healthy Mus musculus mice with a weight range of 20-30g. House the mice in a

clean cage with standard feeding and environmental conditions. Prior to the experiment, shave

the hair on the back of the mice using an electric razor and clean the area with 70% alcohol.

3. Wounding of the mice.

Using sterile surgical scissors, make two full-thickness excision wounds (circular, 5mm

diameter) on the shaven area of each mouse. The wounds should be made at a distance of about 1
cm from each other.

4. Grouping of the mice.

Randomly divide the mice into three groups, each containing ten mice. Group 1 will be

the control group and will receive no treatment. Group 2 and group 3 will be the experimental

groups and will receive daily treatment with 0.5 ml of 5% and 10% Malunggay Moringa Oleifera

leaf extract, respectively.

5. Treatment of the mice.

Starting on the day of wounding, apply 0.5ml of the assigned Malunggay Moringa

Oleifera leaf extract on the wounds of each mouse in the experimental groups using a sterile

syringe and needle. The application should be done daily until complete wound healing is

achieved.

6. Observation and recording.

Observe the wounds daily for signs of healing such as wound contraction, formation of

granulation tissue, and wound closure. Record the wound diameter, the percentage of wound

closure, and the time taken for complete wound healing in each group.

7. Statistical analysis.

Use appropriate statistical tests such as the t-test or ANOVA to analyze the data obtained

from the experiment.

References
Biol, F. C. (2022, April 11). Mouse Models of Liver Parenchyma Injuries and Regeneration.
Retrieved from Frontiers: https://www.frontiersin.org
Farooq Anwar, S. L. (2006, November 06). Moringa oleifera: a food plant with multiple
medicinal uses. Retrieved from https://doi.org/10.1002/ptr.2023
Jed W. Fahey, S. ( 2005). Moringa oleifera: A Review of the Medical Evidence for Its
Nutritional,. Trees for Life , 1-4.
ncbi.nlm.nih.gov. (2018, October 25). Retrieved from International journal of environmental
research and public health: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266783/
Simona Martinotti, E. R. ( 2015, July 22 ). Propolis: a new frontier for wound healing?
Retrieved from Burns & Trauma: https://burnstrauma.biomedcentral.com