Gas Chromatography

SETTING OPERATING CONDITIONS

 First of all, set the Initial Temperature to 60deg. C for 2 minutes in the Method Editor.
Then, Set Ramp 1: 5.0 deg/min to 120 deg. hold for 5.00 min.
Ramp 2: 5.0 deg/min to 200 deg. hold for 5.00 min.
Ramp 3: 5.0 deg/min to 300 deg. hold for 5.00 min.
 Secondly, set the 65 min. Run-time for GC and MS.

SAMPLE PREPARATION

 Methane and Ethane are unable to mix with the crude oil and don’t form the
homogeneous solution.
 Finally, crude oil got mixed with the crude oil to form the dark-brown homogeneous
solution and we used it as the test solution.
 Now, we withdrew the 20 μL and 10 μL test solution through injector and inject it
into two plastic vials filled with 100 μL hexane and labelled it as A and B
respectively.
 Both A and B appeared as light-brown solutions. So, we further diluted the solution
by taking out the 10μL solution from vial A through injector and put it in another
vial(labelled as C) filled with 100μL hexane and we got the crystal clear solution.
 Now, we took out the whole solution from vial C through injector and then attached
the 0.2mm syringe filter to same injector and passed the solution through it and again
kept the solution in the vial C.
 We considered this solution as final injecting solution.
 We used syringe filter to remove the suspended particles from the crystal clear
solution which could cause damage to GC equipment.

INJECTING THE FINAL SAMPLE

 Rinse Micro syringe thrice with hexane. Withdraw the hexane slowly.
(If there are air bubbles, dispense quickly to remove them).
 Further, withdraw 1.0 μL of final injecting solution from Micro syringe and inject the
solution into sample Injection port.
 After injecting, wipe-off the outside of the microsyringe immediately and again rinse
microsyringe with hexane to avoid the clogging of syringe by the solution.
 Finally, take the chromatograph after provided residence time.