CHEMICALS REQUIRED

1. Sodium hydroxide (NaOH)

2. Copper sulphate (CuSO4)
3. Moliscli’s Reagent(C10H70H)
4. Fehling’s Solution A &B
5. Sulphuric acid (H2SO4)
6. Tollen’s Reagent
7. Ammonium Chloride (NH4Cl)
8. Ammonium Hydroxide (NH4OH)
9. Sodium Phosphate (Na3PO4)

PROCEDURE FOR ANALYSIS

Organic tests and Inorganic tests done to find the presents of the different in chocolates. Tests for identification of Calcium and
Magnesium (Good Substances) and also tests for identification of Lead and Nickel (Poisonous Substances) were also done.

TEST FOR PROTEIN

EXPERIMENT OBSERVATION INFERENCE
Sml of each sample if chocolate taken in different test tube. Appearance of violet Presence of PROTEIN in
Add 1 pellet of NaOH to each. Add 1-2 drops of CuS04 solution to each coloration observed. the sample.
case.

RESULT

All samples studied showed that they contain PROTEIN.

(dark chocolate.... chocolate cream.... Milky bar...... milk chocolate bar....

Cadburys bar)

TEST FOR FAT

EXPERIMENT OBSERVATION INFERENCE
Take a small sample of each chocolate on different pieces of filter paper. Appearance of violet Presence of FAT
Fold and unfold the paper on heating was to crush the sample over a flame colouration observed. in the sample

TEST FOR SUGAR

EXPERIMENT OBSERVATION INFERENCE
5ml of each sample if chocolate taken in different test tube. A purple ring is Presence of SUGAR
Add 1ml water to each then add few drops of moliscli’s reagent (alpha naphthol formed at the in the sample.
in alcohol.) top.
Then add con. H2SO4 drops along inner edge of the test tube.
Mix 2ml of Fehling’s A & B in different test tubes. Add a pinch of the chocolate Red-brown Presence of SUGAR
into each test tube. precipitate was in the sample.
Then the solution in the water bath. obtained.
2ml of Tollen’s Reagent was taken in different test tube. A silver mirror Presence of SUGAR
Add a pinch of the chocolate into each test tube. surface is in the sample.
Then the solution in the water bath. obtained.

TEST FOR CALCIUM

EXPERIMENT OBSERVATION INFERENCE
A mixture of NH4Cl + NH4OH+(NH4)2CO3 A white Presence of CALCIUM
precipitate in the sample.
was obtained.
With help of a glass rod each sample of chocolate solution placed on different Brick red Presence of CALCIUM
watch glass. colour flame in the sample.
Add a drop of con.HCl and a paste is made on each sample. was obtained.
This paste from each sample is taken on the tip of new glass rod and shown to blue
flame of spirit lamp.

TEST FOR IRON

EXPERIMENT OBSERVATION INFERENCE
A mixture of NH4Cl + NH4OH is made. This added to each sample of No brown Absence of IRON in the
chocolate solution taken in different test tubes. precipitate was sample.
obtained.

TEST FOR MAGNESIUM

EXPERIMENT OBSERVATION INFERENCE
A mixture of NH4Cl + NH4OH +Na3PO4 is made. This is added to each sample of No white Absence of
chocolate solution taken in different test tubes. precipitate was MAGNESIUM in the
obtained. sample.

TEST FOR NICKEL

EXPERIMENT OBSERVATION INFERENCE
A mixture of NH4Cl + NH4OH is made. This is added to each sample of No black precipitate was Absence of NICKEL
chocolate solution taken in different test tubes. obtained. in the sample.
Pass H4S gas through the solution.