Phytochemical Screening

A. TEST FOR ALKALOIDS

Results:
Mayer’s Reagent Formation of light brown
precipitate

Wagner’s Reagent Formation of brown colored

precipitate

Confirmatory test
Mayer’s Reagent Formation of light green color

Wagner’s Reagent Formation of green color

B. TEST FOR CARDENOLIDES AND BUFADIENOLIDES

Results:
Keller-Killiani Test Formation of greenish-brown
color

C. TEST FOR FLAVONOIDS

Results:
Bate-Smith and Metcalf Test Formation of red color

Cyanidin Test Upper layer- No color change

Lower layer- Formation of light
yellow color with brown
precipitate.

D. TEST FOR TANNINS

Results:
Gelatin Test Formation of reddish-orange
color

Ferric Chloride Test Formation of light brown color

 E. TEST FOT ANTHRAQUINONE
Results:
Borntrager Test Upper layer- No color change
Lower layer- Formation of light
orange color

Modified Borntrager Test Upper layer- Formation of light

green color
Lower layer- Result became
clear

F. TEST FOR CARBOHYDRATES

Results:
Fehling’s Test Formation of Green color and
brick red precipitate

Moore’s Test Formation of yellow color and

brown precipitate

G. TEST FOR RESIN

Results:
Phloroglucinol Test Formation of dark brown color
with dirty white precipitate

H. TEST FOR FIXED OILS AND VOLATILE OILS

Results:
Stain Test There is a formation of stain