Name: ___________________________________ Period: ___________ Date: ___________

2.5 Stickleback Evolution Virtual Lab  

Lesson Objective:​​Today I will be able to: ​Analyze​ the pelvic structures of
stickleback fish collected from two lakes to determine which population (Bear Paw or
Frog Lake) has experienced a loss of pelvic structure and make a prediction about why
this happened.

Criteria for Success

❏ Determine if pelvic structure is Complete, Reduced, or Absent
❏ Determine ​which population has lost pelvic structure over time
❏ Identify 3 pieces ​of ​relevant/appropriate evidence ​to support my claim and help explain why this happened

Do Now
1. In my own words, ​ phenotype​is…
____________________________________________________________________________________________
____________________________________________________________________________________________

2. Having a pelvic structure is​ dominant​over not having a pelvic structure. Which of the following​ best describes
this dominant trait in stickleback fish​?
a. A ​homozygous recessive (pp) stickleback fish​​will​ have a pelvic structure
b. A​ heterozygous (Pp)​stickleback fish ​will not​ have a pelvic structure
c. A ​homozygous dominant (PP) ​stickleback fish ​will​ have a pelvic structure
d. A ​homozygous dominant (PP) and heterozygous​​(Pp)​stickleback fish ​will​ both show a pelvic
structure.

3. Based on your knowledge, what do you think is an advantage of having a pelvic structure? What is a disadvantage
of having a pelvic structure?
____________________________________________________________________________________________
____________________________________________________________________________________________

TEAM ROLES: Virtual Lab Link:​ ​Stickleback Evolution Lab

Manager Set up and run the virtual lab

Timekeeper Keep Track of time and make sure to check in with your members about time

Leader  Leads discussion,​makes sure everyone is on task at all time and participating, kind to
one another, using academic language, and gets packet signed.

Recorder Makes sure ​all group members​ write down the information on the data tables.

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 Name: ___________________________________ Period: ___________ Date: ___________
Part 1: Tutorial 1 
1. In tutorial 1, you will practice ​scoring preserved and stained stickleback fish​.
2. Click the tray of fish to begin.
3. For each sample stickleback fish, analyze its pelvic structure phenotype. If the​ pelvic structure​ is:
Complete​, write ​C
Reduced​, write ​R
Absent​, write ​A

(2-3 min) Actual ​Timekeeper time​:​ ​ __________

Fish 1 2 3 4 5 6 7 8 9 10
sample #

Pelvic
structure

Your Score: __________________________

LEADER ​Discussion Questions: (Make sure to discuss as a group, encourage ALL members to participate)
a. How do you determine whether a pelvic structure is ​Complete, Reduced or Absent?
b. How do you think the pelvic structure helps the stickleback fish survive?
c. What would need to happen for the pelvic structure phenotype to disappear?

 
Part 2: Experiment 1 Part 1 - Staining the Fish 
1. Press the blue gloves to get started.

2. Click on

 
 
 
 
 
 
 
 
 
 
 
 
 
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Name: ___________________________________ Period: ___________ Date: ___________
Part 3: Experiment 1 Part 2- Scoring the Fish 
1. Click the jar of stained fish labeled ​Bear Paw Lake​to begin.
2. Follow the directions on the lab to prepare the fish. Then make sure to analyze the pelvic structure of each fish
and fill out the chart below.
3. Repeat the steps for the ​Frog Lake.

(6-8 min) Actual ​Timekeeper time​:​ ​ __________

Stickleback Bear Paw Lake Stickleback Fish  Stickleback Frog Lake Stickleback Fish 
Fish Pelvic Structure Phenotype: Fish Sample Pelvic Structure Phenotype:
Sample # Complete, Reduced, or Absent? # Complete, Reduced, or Absent?

1 1

2 2

3 3

4 4

5 5

6 6

7 7

8 8

9 9

10 10

11 11

12 12

13 13

14 14

15 15

16 16

17 17

18 18

19 19

20 20

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Name: ___________________________________ Period: ___________ Date: ___________
Part 4: Analyze the Data: ​Fill out the data table below:
Phenotype Bear Paw Lake Frog Lake

# of fish with ​Complete Pelvic Structure

# of fish with ​Reduced Pelvic Structure

# of fish with ​Absent Pelvic Structure

 
LEADER ​Discussion Questions: (Make sure to discuss as a group, encourage ALL members to participate)
a. Looking at your data, which pelvic structures phenotype (Complete, Absent, Reduced) of stickleback
fish were most prominent in ​Bear Paw Lake​?

b. Looking at your data, which pelvic structures phenotype (Complete, Absent, Reduced) of stickleback
fish were most prominent in ​Frog Lake?

c. In terms of pelvic structure phenotypes (Complete, Reduced, Absent), why​are the Bear Paw Lake
stickleback fish and the Frog Lake stickleback fish ​different from one another?​​What ​environmental
factors can you predict​might be responsible for these differences? Come up with at least 3 possible
environmental factors.

Part 5: ​Written Response

Claim:​Which populations of stickleback fish has lost its pelvic structure over time (absent/reduced), the Bear Paw
Lake stickleback fish, the Frog Lake stickleback fish, or both?
______________________________________________________________________________________________
______________________________________________________________________________________________
Part 6: ​Evidence
1. Now that you have a claim, start to gather 3 pieces of evidence that ​BEST​​support your claim and/or can
start to help explain why this population may have lost their pelvic structures over time​.
2. Open the Evidence envelope for the Population you selected.
3. LEADER ​Discussion Questions: (Make sure to discuss as a group, encourage ALL members to
participate)
4.Look at the evidence cards one by one. Discuss any of the  Evidence Criteria for Success  
following: 
a. What does the evidence card show? What does it ❏ I selected 3 different pieces of evidence that ​BEST ​support
mean? the claim
b. The evidence that best supports my claim about
❏ I can explain what the evidence card selected means/shows
stickleback fish losing pelvic structures is…
c. My first piece of evidence is …
❏ I can explain how this piece of evidence helps to support
d. Another piece of evidence is… the claim ​OR​ how it helps to predict why this population
e. This evidence shows that… of stickleback fish no longer have pelvic structures
f. Based on this evidence I can conclude that…
g. This is important because ... ❏ I am using academic language/vocab words

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 Name: ___________________________________ Period: ___________ Date: ___________
Part 6: ​Selection of Evidence to Support Claim Rubric
Skill 4 3 2 1
Using Various My claim ​answers​ the My claim ​answers​ the question My claim ​answers​ the My claim is ​unclea​r or
Sources of question about which about which population (Bear question about which does not​ answer the
Evidence to population (Bear Paw Lake Paw Lake or Frog Lake) has population (Bear Paw Lake question about which
Support a or Frog Lake) has lost the lost the pelvic structure over or Frog Lake)has lost the population (Bear Paw
pelvic structure over time time ​AND​ is supported by 2 pelvic structure over time Lake or Frog Lake) has
Claim
AND​ is supported by 3 or DIFFERENT and relevant BUT is only supported by lost the pelvic structure
more ​DIFFERENT and sources​ of evidence. 1 piece of evidence or does over time ​AND​ is ​NOT
relevant ​sources​ of not use ​DIFFERENT supported by ​ANY
evidence AND explains sources of evidence. EVIDENCE/OR THE
how this piece of evidence EVIDENCE IS
supports the claim. INACCURATE
Think-Pair-Share: ​What is the difference between a 3 and a 4?

Evidence #1 and Reasoning: ​My first piece of evidence to help support or explain my claim is…
_____________________________________________________________________________________________
______________________________________________________________________________________________
______________________________________________________________________________________________
______________________________________________________________________________________________
______________________________________________________________________________________________
______________________________________________________________________________________________

Evidence #2 and Reasoning: ​In addition, another piece of evidence to help support or explain my claim is…
_____________________________________________________________________________________________
______________________________________________________________________________________________
______________________________________________________________________________________________
______________________________________________________________________________________________
______________________________________________________________________________________________
______________________________________________________________________________________________

Evidence #3 and Reasoning: ​Furthermore,

_____________________________________________________________________________________________
______________________________________________________________________________________________
______________________________________________________________________________________________
______________________________________________________________________________________________
______________________________________________________________________________________________
______________________________________________________________________________________________  
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Name: ___________________________________ Period: ___________ Date: ___________

2.5 Independent Tasks  

Working ​SILENTLY​ AND ​INDEPENDENTLY. ​RAISE YOUR HAND IF YOU HAVE A QUESTION

Part 1: “Video #1: Making of the fittest”

1. Watch the ​Making of the fittest: Stickleback Evolution Video
2. Write down 7 facts you learned from the video

1.

2.

3.

4.

5.

6.

7.

​Part 2: Punnett Squares Vocab

1. Use the link ​Punnett Squares Vocab​ ​ to fill the chart below:

WORD DEFINITION EXAMPLE/SENTENCE/IMAGE

Punnett square

Allele

Hybrid

Purebred

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Name: ___________________________________ Period: ___________ Date: ___________

Part 3:DNA and Genes Video

Directions: ​Watch the ​DNA, Chromosomes, Genes and Traits Video​ ​and answer the following questions.

1. Who is Spike?

2. What is heredity?

3. What are some of Spike’s traits?

4. What does DNA stand for?

5. What are the 3 parts of nucleotides?

6. What is the most important part of the nucleotide? Why?

7. What are the 4 bases of DNA? How do they pair up?

8. What holds the bases together?

9. Proteins coded for by genes play a wide variety if roles besides just your eye color? What are some
things proteins are involved in?

10. What is gene regulation?

11. How many chromosomes do humans have?

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Name: ___________________________________ Period: ___________ Date: ___________

Part 4:DNA and Genes Video

Directions: ​Watch the ​Alleles and Genes Video ​and answer the following questions:

1. What is PTC?

2. What does PTC taste like?

3. What does each chromosome pair have?

4. What is the gene locus?

5. What are alleles?

6. What are the 2 possible genotypes for PTC tasters?

7. What is the genotype for the people cannot taste PTC?

8. What do the parent genotypes need to be?

9. What can punnett squares be used for?

10. What is Polydactyl?

Part 5: Punnett Squares Guinea Pigs

Watch the ​Punnett Squares Guinea Pigs​ ​ and answer the following questions:
1. ​What are alleles?

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Name: ___________________________________ Period: ___________ Date: ___________
2. Does a ​hairless​ guinea pig have​ recessive or dominant alleles?

3. What does it mean by a ​recessive allele?

4. What does it mean by a ​dominant allele?

5. What is a ​monohybrid cross?

6. What are the ​steps to fill out a Punnett Square?

7. What are the ​genotypes of the offspring? What does the 1:2:1 ratio mean?

8. What is the​ phenotype ratio?​ What does ​3:1 ratio mean?

9. What are ​probabilities?

7. Copy the chart below. After watching the video, you should be able to fill out the chart below using the information
provided in the video. If you need to watch the video again, here is the link: ​Punnett Squares Guinea Pigs
Image Genotype Heterozygous or Phenotype
homozygous

HH

1.

Hairless

2.

Heterozygous

3.

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Name: ___________________________________ Period: ___________ Date: ___________
8. If a baby ​girl guinea pig looks almost identical to its mother​, does this then mean that it inherited more alleles
from its mother? Explain. (​Hint: Think about the vocabulary words dominant and recessive.)

Part 6: SNPs
Watch the ​SNPs video​ and answer the following questions:
1. What is a ​genome?

2.How many base pairs is the human genome made up of?

3. What is an​ SNP​?

4. How many​ SNPs​ are there in the human genome?

Part 7: Finish Work

1. Make sure all sections of packet 2.4 Pre Lab notes is complete (Monday’s Packet)
2. Complete any incomplete sections from 2.2 - 2.3 packets
3. Work on an Achieve Article/iReady/DreamBox/Moby Max

 
 
 
 
 
 
 

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Name: ___________________________________ Period: ___________ Date: ___________

 
Peer-Feedback Form 
Name of student you are evaluating: _____________________

Peer Feedback Tasks Rate your Justify your rating

partner’s
evidence 1 to 4

1. Does the claim answer the question? ________________________________

● Which stickleback fish populations have​ lost the pelvic ________________________________
structure over time​? ​(The Bear Paw Lake or Frog Lake 1 2 3 4 ________________________________
stickleback fishes?) ________________________________
________________________________

2. Evidence #1 ________________________________
________________________________
● This piece of evidence supports the claim ​OR​ it helps ________________________________
to predict why this population of stickleback fish no
longer have pelvic structures
1 2 3 4 ________________________________
● Response uses academic language ________________________________
● Written in complete sentences ________________________________
● This is one of the ​STRONGEST ​pieces of ________________________________
evidence to help support this claim

3. Evidence #2 ________________________________
● This piece of evidence supports the claim ​OR​ it helps ________________________________
to predict why this population of stickleback fish no ________________________________
longer have pelvic structures
● Response uses academic language
1 2 3 4 ________________________________
● Written in complete sentences ________________________________
● This is one of the ​STRONGEST ​pieces of ________________________________
evidence to help support this claim ________________________________
________________________________

4. Evidence #3 ________________________________
● This piece of evidence supports the claim ​OR​ it helps ________________________________
to predict why this population of stickleback fish no ________________________________
longer have pelvic structures
● Response uses academic language
1 2 3 4 ________________________________
● Written in complete sentences ________________________________
● This is one of the ​STRONGEST ​pieces of ________________________________
evidence to help support this claim ________________________________

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