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The Microscope
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A microscope is used for looking at objects that are (00 sail to see with the nakec! eve,
The lenses in the microscope meeni'y objects (make them |ook bigger) so that you can see them.
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objective lenses
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Follow These Easy Steps to Using a Light Microscope
1) Carry your microscope by the hancle.
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2) Place it near a lam or window, and angle the Don't
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reflectdirect sunlight
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into the microscope — it
mirror so light shines through the hole in the stage.
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3) Clip a slice onto the sieve. The slice should have the could damage your eyes.
object(s) you want to look at siuck ioii. 2
4) Select the lowest powered objeciive lens.
5) Luin the roueh tocusine knob to move
the objective lens down to just above the slide.
6) Look down the evepiece lens and adjusi ihe focus using the fine focusing knob.
7)Keen adjusiing until you get a clear imave of whatever's on the slide.
DON’T BREAK THE SLIDE yswitch
8) If to see theslide with greater
ou needahigher magnification,
to sowerec! objectivelens (a longer one).
Always turn the fine focusing knob 9) Now refocus the microscope (repeat steps 5 to 7).
so that the objective lens is moving
away from the slide —so the lens
and slide don’t crash together.
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2) Cells can be seen through a microscove (see previous page) —
but it helps if you siain them first (using a coloured dive).
An Animal Cell
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ONY PLANTS have:
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2) AVacuole:
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EUGLENA: :
chloroplast AMOEBA: : |
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Here's a rather jolly example from a olani.
Don't forget that the sequence applies just as well to animels. ey
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palisade C115... ...make up palisade [55U-... ..which, with other ..which, with more leaves
tissues, makes up a_—_—_and other organs, makes up
A palisade cell is just the name leaf (an ORGAN)... the shoot system (an O2CA!
for a particular type of plant cell. SYSTEM). Different organ
systems make up full plant
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Stuff Moves Into and Out of Cells by Diffusion
1) Cells need things like glucose (a sugar) and oxveen to suivive.
They also need to get ric! of wasie products, like carbon cioxicde.
2) These materials all move inio or oui of cells by a process called citiusion.
3) Diffusion is where a substance moves from an area of high conceniration (where there’s lois of ii
to an area of low concentration (where there's ess oi ii) — just like glucose in this diagram...
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(lots of glucose) 1 © (not much glucose)
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cell membrane
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Respiration is one of the most importantlite orocesses there is. It’s worth learning really well.
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Lactic acid can duilc up in your muscles during exercise and can be painiul.
2) In microorecnisms like yeasi, anaerobic respiration produces carbon cioxice and einanol (alcohol):
respiration,
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glucose .
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Warm-Up Questions
1) Why would you stain a cell before looking at it under a microscope?
2) Name three structures that are found in both plant and animal cells.
Describe what they all do.
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What is the difference between a tissue and an organ?
4) Which process is responsible for the movement of glucose from an area of high
concentration to an area of low concentration?
5) Which sort of respiration involves oxygen? Write the relevant word equation.
6) Which sort of respiration is the most efficient?
Practice Questions
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(a) Name the cell paris labelled (i)-(iv) on the diagram.
(4 marks)
(ob) Name two structures that are found in plant cells, but not in animal cells.
(2 marks)
Practice Questions
...
3 (a) Use the following words to complete the gaps in the sentences below.
a tissue cells an organ
(3 marks)
4 Alana’s class are investigating the cells in onion skin using light microscopes.
Alana collects a microscope from the teacher and positions it near a window.
(a) Light has to enter the microscope for it to work.
(i) Which part of the microscope can be adjusted to allow light in?
(1 mark)
(ii) Which kind of light should not be allowed to enter the microscope?
Explain your answer.
(2 marks)
(b) Alana clips a slide with a piece of onion skin stuck to it onto the stage.
(i) Describe the steps she should take to get a clear image of the
onion cells.
(4 marks)
(ii) Alana would like to make the image of the onion cells bigger.
Describe how she can do this.
(2 marks)