74

Lampiran 15. Perhitungan Rendemen Ekstrak

Berat ekstrak yang di dapat

% Rendemen = X 100%
Berat simplisia yang di ekstraksi

= 261,3 g
X 100%
2000 g

= 13,065%
 75

Lampiran 16. Perhitungan Kadar Senyawa Larut Etanol

Bobot Kadar
Cawan Cawan+Ekstrak Akhir Ekstrak
Replikasi Rata-rata
kosong (g) Akhir (g) Ekstrak Larut Air
(g) (%)
1 74,5 75,2 0,7 14
2 71,2 72,0 0,8 16 14%
3 70,7 71,3 0,6 12

% Kadar = Bobot ekstrak akhir

X 100%
Bobot ekstrak awal
0,7 g
1. % Kadar senyawa larut etanol Rep 1 = X 100% = 14%
5g

0,8 g
2. % Kadar senyawa larut etanol Rep 2 = X 100% = 16%
5g

0,6 g
3. % Kadar senyawa larut etanol Rep 3 = X 100% = 12%
5g

14% + 16% + 12%

Rata – rata = = 14%
3

Standar Deviasi (SD) = 2

 76

Lampiran 17. Perhitungan Kadar Senyawa Larut Air

Bobot Kadar
Cawan Cawan+Ekstrak Akhir Ekstrak
Replikasi Rata-rata
kosong (g) Akhir (g) Ekstrak Larut Air
(g) (%)
1 64,3 64,9 0,6 12
2 70,7 71,3 0,6 12 12,67%
3 71,2 71,9 0,7 14

% Kadar = Bobot ekstrak akhir

X 100%
Bobot ekstrak awal
0,6 g
1. % Kadar senyawa larut air Rep 1 = X 100% = 12%
5g

0,6 g
2. % Kadar senyawa larut air Rep 2 = X 100% = 12%
5g

0,7 g
3. % Kadar senyawa larut air Rep 3 = X 100% = 14%
5g

12% + 12% + 14%

Rata – rata = = 12,67%
3

Standar Deviasi (SD) = 1,154701

 77

Lampiran 18. Perhitungan Susut Pengeringan

Berat Berat
Berat
Ekstrak wadah + Susut
Replikasi Wadah Rata-rata
Awal Ekstrak pengeringan (%)
(g)
(g) Akhir (g)
1 14, 6521 1,1482 15,2072 51,6548 51,9667
2 14,5175 1,1782 15,0815 52,1304 %
3 14,1050 1,0709 14,6178 52,1150

Berat Susut Pengeringan

Susut Pengeringan (%) = X 100%
Berat ekstrak awal

1. Susut Pengeringan (%) Rep 1 = 0,5931

X 100% = 51,6548%
1,1482
0,6142
2. Susut Pengeringan (%) Rep 2 = X 100% = 52,1304%
1,1782

0,5581
3. Susut Pengeringan (%) Rep 3 = X 100% = 52,1150%
1,0709

Rata-rata = 51,6548% + 52,1304% + 52,1150%

= 51,9667%
3

Standar Deviasi (SD) = 0,270252

 78

Lampiran 19. Perhitungan Bobot Jenis

Piknometer Pikno+ Pikno+ Ρ Air Bobot

Rata-
Replikasi kosong (g) Ekstrak (g) Air (g) (g/mL) Jenis
rata
(g/mL)
1 15,1370 27,6443 24,8569 0,9720 1,2507
1,2507
2 15,1370 27,6436 24,8569 0,9720 1,2507
g/mL
3 15,1370 27,6445 24,8569 0,9720 1,2508

W2 – W0
d= X bj Air
W1 – W0

27,6443-15,1370
1. Bobot Jenis Rep 1 = X 0,9720 = 1,2507 g/mL
24,8569-15,1370
27,6436-15,1370
2. Bobot Jenis Rep 2 = X 0,9720 = 1,2507 g/mL
24,8569-15,1370

27,6445-15,1370
3. Bobot Jenis Rep 3 = X 0,9720 = 1,2508 g/mL
24,8569-15,1370

Rata-rata = 1,2507g/mL + 1,2507g/mL + 1,2508g/mL = 1,2507 g/mL

3

Standar Deviasi (SD) = 0,000057

 79

Lampiran 20. Perhitungan Kadar Air

Wadah+ Ekstrak Wadah+ Berat air

Kadar
Replikasi ekstrak (g) ekstrak yang Rata-rata
Air (%)
(g) akhir (g) hilang (g)
1 64,8074 2,3607 64,2438 0,5636 23,8743
2 54,6625 2,0537 54,1785 0,4840 23,5672 23,7648%
3 68,5205 2,1402 68,0100 0,5105 23,8529

Berat Air yang Hilang

Kadar Air = X 100%
Berat Ekstrak Awal

0,5636
1. Kadar Air Rep 1 = X 100% = 23,8743%
2,3607

0,4840
2. Kadar Air Rep 2 = X 100% = 23,5672%
2,0537

0,5105
3. Kadar Air Rep 3 = X 100% = 23,8529%
2,1402

Rata-rata = 23,8743% + 23,5672% + 23,8529% = 23,7648%

3

Standar Deviasi (SD) = 0,171461

 80

Lampiran 21. Perhitungan Kadar Abu Total

Berat Berat Cawan +

Cawan (g) Ekstrak Ekstrak
Replikasi Awal (g) setelah Kadar Abu (%) Rata-rata
diabukan
(g)
1 25,5061 1,1495 25,7282 19,3214
2 24,3539 1,2662 24,6043 19,7757 19,5572%
3 24,3548 1,2266 24,5949 19,5744

W2 – W0
Kadar Abu Total (%) = X 100%
W1

25,7282 – 25,5061
1. Kadar Abu Total Rep 1 = X 100% = 19,3214%
1,1495

24,6043 – 24,3539
2. Kadar Abu Total Rep 2 = X 100% = 19,7757%
1,2662

24,5949 – 24,3548
3. Kadar Abu Total Rep 3 = X 100% = 19,5744%
1,2266

19,3214% + 19,7757% + 19,5744%

Rata-rata = = 19,5572%
3

Standar Deviasi (SD) = 0,227640

 81

Lampiran 22. Perhitungan Kadar Abu Tak Larut Asam

Berat
Cawan +
Berat Kadar
Abu
Berat Berat Kertas Abu Tidak
Replikasi yang C*0,0076 Rata-rata
cawan ekstrak Saring Larut
Tak
(g) Asam (%)
Larut
Asam (g)
4,26208 x
1 25,5061 1,1495 25,5131 0,5608 0,2382
10-3
4,32136 x 0,2310%
2 24,3539 1,2662 24,3610 0,5686 0,2195
10-3
4,31528 x
3 24,3548 1,2262 24,3620 0,5678 0,2353
10-3

Kadar Abu Tak Larut Asam (%) = W2 – (C X 0,0076) – W0

X 100 %
W1

25,5131 – (0,5608 X 0,0076) – 25,5061

X 100%
1. Kadar Abu Tak Larut Asam Rep 1 = 1,1495

= 0,2382%

24,3610– (0,5686 X 0,0076) –24,3539

X 100%
2. Kadar Abu Tak Larut Asam Rep 2 = 1,2662
= 0,2195%

24,3620– (0,5678 X 0,0076) – 24,3548

X 100%
3. Kadar Abu Tak Larut Asam Rep 3 = 1,2262
= 0,2353%

Rata-rata = 0,2382% + 0,2195% + 0,2353% = 0,2310%

3

Standar Deviasi (SD) = 0,010064

 82

Lampiran 23. Perhitungan Kadar Pb

Replikasi Ekstrak Pengen Volume Pembacaan Kadar Pb Rerata

(g) ceran (mL) (mg/L) (mg/kg) (mg/kg)
1 2,0483 1 50 0,097 2,36782 2,3473
2 2,0493 1 50 0,097 2,36666 mg/kg
3 2,0587 1 50 0,095 2,30728

Kadar Pb (mg/kg) = Pembacaan

X Volume X Pengenceran
Berat Ekstrak

1. Kadar Pb Rep 1 = 0,097

X 50 X 1 = 2,36782 mg/kg
2,0483
0,097
2. Kadar Pb Rep 2 = X 50 X 1 = 2,36666 mg/kg
2,0493

3. Kadar Pb Rep 3 = 0,095

X 50 X 1 = 2,30728 mg/kg
2,0587

Rata-rata = 2,36782 mg/kg + 2,36666 mg/kg + 2,30728 mg/kg

= 2,3473 mg/kg
3

Standar Deviasi (SD) = 0,034623

 83

Lampiran 24. Perhitungan Kadar Hg

Replikasi Ekstrak Pengen Volume Pembacaan Kadar Pb Rerata

(g) ceran (mL) (mg/L) (mg/kg) (mg/kg)
1 2,0483 1 50 <0,0002 <0,00488 <0,0049
2 2,0493 1 50 <0,0002 <0,00488 mg/kg
3 2,0587 1 50 <0,0002 <0,00486

Pembacaan
Kadar Hg (mg/kg) = X Volume X Pengenceran
Berat Ekstrak

1. Kadar Hg Rep 1 = 0
X 50 X 1 = 0 mg/kg
2,0483
0
2. Kadar Hg Rep 2 = X 50 X 1 = 0 mg/kg
2,0493

3. Kadar Hg Rep 3 = 0
X 50 X 1 = 0 mg/kg
2,0587

Rata-rata = 0 mg/kg + 0 mg/kg + 0 mg/kg

= 0 mg/kg
3

Standar Deviasi (SD) = 0

 84

Lampiran 25. Perhitungan Kadar As

Replikasi Ekstrak Pengen Volume Pembacaan Kadar Pb Rerata

(g) ceran (mL) (mg/L) (mg/kg) (mg/kg)
1 2,0483 1 50 <0,003 <0,07323 <0,0731
2 2,0493 1 50 <0,003 <0,07320 mg/kg
3 2,0587 1 50 <0,003 <0,07286

Kadar As (mg/kg) = Pembacaan

X Volume X Pengenceran
Berat Ekstrak

1. Kadar As Rep 1 = 0
X 50 X 1 = 0 mg/kg
2,0483
0
2. Kadar As Rep 2 = X 50 X 1 = 0 mg/kg
2,0493

3. Kadar As Rep 3 = 0
X 50 X 1 = 0 mg/kg
2,0587

Rata-rata = 0 mg/kg + 0 mg/kg + 0 mg/kg

= 0 mg/kg
3

Standar Deviasi (SD) = 0