CASTILLO, Nicole Bernardine C.

Parasitology Lec
Sec 15
MTY-1206
HARADA MORI TECHNIQUE
Principle  Introduced by Harada and Mori in 1995
 This techinque uses a filter paper, fecal material,
tap water, and a centrifuge.
 This technique is used in detecting light infection
with hookworm, and in facilitating species of
helminths
 This technique is also used for culturing and
recovering nematode larvae
 The fresh fecal sample placed on a filter paper
strip is submerged in a test tube containg water.
The water continuously soak the paper strip by
capillary action. This helps to induce the hatching
of ova and the development of larvae
 Has greater sensitivity compared to DFS and FECT
 Has a lower detection rate than Baermann and
agar plate culture
Specimen of  Fresh stool sample
choice
Materials  Disposable glass or plastic pipettes
 Glass slides
 Coverslips
 Filter paper
 Wooden applicator sticks (nonsterile)
 15 mL conical tube
 Forceps
 Microscope
 Personal protective equipment
Quality control 1. Follow routine procedures to ensure reliable
results
2. Review larval diagrams for confirmation of larval
indentification
3. Microscope should be calibrated
Procedures 1. Wear PPE
2. Cut a narrow strip of filter paper, 3/8 by 5 inches
will do. Taper it slightly at one end. Smear 0.5 to
1 g of fecal sample on the center of the strip
3. Add 3 to 4 mL of distilled water to a 15 mL conical
centrifuge tube
 4. Insert the filter paper strip into the tube so that
the tapered end is near the bottom of the tube. A
cork stopper or a cotton plug may be used bot is
not necessary.
5. Allow tube to stand upright in a rack at 25 to 28°C.
Add distilled water to maintain the original level
6. Keep the tube for 10 days and check daily by
withdrawing a small amount of fluid from the
bottom of the tube. Prepare a smear on a glass
slide, cover with a coverslip and examine with the
10 X objective
7. Examine the larvae for motility and typical
morphological features to reveal whether
hookworm, Strongyloides, or Trichostrongylus
larvae are present
Results  Larval nematodes may be recovered
 If Strongyloides organisms are present, free-living
stages and larvae may be found after several
days in culture

Reference:
Leber, A. L. (2016). Clinical Microbiology Procedures Handbook (4th ed.).
Washington, DC: ASM
Press.