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q16
Convenient kit formats
Easy Kit Advanced Kit Standard Kit genesigPLEX Kit genesigPLEX Kit genesigMYPLEX
Human Veterinary Kit
genesig q16 All other instruments: Thermo, Bio-Rad, Roche, QIAGEN etc ...
Multiplex qPCR Y Y Y
Target primer and probe mix Y Y
Positive control Y Y Y Y Y Y
Separate Endogenous control Y
Internal extraction control (IEC) primer and probe mix Y
Target/IEC primer and probe mix Y Y
Target/endogenous control primer and probe mix Y Y
Lyophilised master mix Y Y Y Y
Reaction tubes Y
Resuspension buffers Y Y Y Y Y Y
Kit size (tests) 50 100-150 100-150 100 100 100
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The WORLDS most Affordable qPCR machine
System specifications:
genesig-q16 real-time PCR System
Ultra-
Automated data analysis
No moving parts
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qPCR test Respiratory
infections
Respiratory
infections
kits human continued
pathogen •
•
•
•
Adenovirus type B
Adenovirus type C
Adenovirus type D
Adenovirus type F&G
• Methicillin-resistant Staphylococcus aureus
• MRSA with Staphylococcal cassette chromosome
mec
Multiplex Kit
• genesigPLEX HAV/HEV
• genesigPLEX HBV/HCV/HIV1/HIV2
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Gastrointestinal Gastrointestinal Vector-borne
infections infections diseases
continued continued
Human parasites
genesigMYPLEX STD - Select any 2/3-way multiplex
combination from these 10 key STD viruses
• Cytomegalovirus (HHV5)
• Enterovirus • Chlamydia trachomatis
• Haemophilus influenzae • Gardnella vaginalis
• Herpes simplex type 1 (HHV1) • Herpes simplex type 1 and 2 • Ascaris lumbricoides/ascaris suum
• Herpes simplex type 1 and 2 (HHV1&2) • Mycoplasma genitalium • Acanthamoeba species
• Herpes simplex type 2 (HHV2) • Mycoplasma hominis • Balamuthia mandrillaris
• Leptospirosis • Neisseria gonorrhoeae • Cystoisospora belli
• Lyme disease • Treponema parvum • Entamoeba histolytica
• Neisseria meningitidis • Trichomonas vaginalis • Giardia intestinalis
• Streptococcus pneumoniae • Ureaplasma parvum • Leishmania infantum
• Ureaplasma urealyticum • Leishmania major
• Leishmania
• Leishmania tropica
• Naegleria species
• Naegleria Fowleri
• Plasmodium falciparum
• Plasmodium knowlesi
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Human parasites Multiplex kits
continued
Others
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qPCR test Pathogen
contamination
kits food,
water and •
•
•
Bacillus cereus E33
Brucella abortus
•
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•
Salmonella enterica
agriculture •
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•
•
Brucella genus (all species)
Campylobacter Coli
Campylobacter Jejuni
Coxiella burnetii
•
•
•
•
Shigella (all species)
toxigenic subspecies of Vibrio cholerae
Cystoisospora belli
qPCR testing methods are proven • • Propionibacterium acnes
to be the fastest and most accurate • Cyclospora cayetanensis • Yersinia enterocolitica
• Enterococcus faecalis
way for screening water and food.
• Enterococcus faecium
We offer highly sensitive kits for food • Enterohemorrhagic Escherichia coli
borne pathogens, GMO detection, • Enteroinvasive Escherichia coli
agriculture, meat speciation, allergen •
• Enterotoxigenic Escherichia coli
testing and water contaminants. • Escherichia coli (all strains)
• Escherichia coli 0157:H7
Speciation Allergen
Speciation continued
• Venison •
• Warthog
Meat speciation kits
•
• Fish speciation kits
• • Atlantic Cod
• • Coley
• • Haddock
• • Pollock
• • Whiting
•
• AnimalFINDER for detection of
• highly processed samples/clean
•
•
down validation
• Chicken
• Cod
• Cow
• Horse
• Pig
• Sheep
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Others
• genesig Easy kit for hop resistant Lactobacillus and Pediococcus species
• genesig Easy kit for Pediococcus genus
• genesig Easy kit for Pectinatus genus
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qPCR test Veterinary pathogen
kits
kits veterinary
pathogens •
•
•
•
Acholeplasma laidlawii
Acute bee paralysis virus
Aeromonas hydrophila
Aeromonas salmonicida
•
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•
Camelpox virus
Campylobacter Coli
Campylobacter coli and Campylobacter jejuni
Campylobacter fetus
• African Horse Sickness Virus • Campylobacter fetus subspecies venerialis
The veterinary range is currently the • NEW! African swine fever virus • Campylobacter Jejuni
• African Trypanosomiasis • Candidatus Branchiomonas cysticola
fastest growing part of the genesig • Agapornis species • Candidatus Mycoplasma haemominutum
portfolio. qPCR based veterinary • Aleutian Disease Virus • Candidatus Mycoplasma turicensis
kits attract a lot of attention and this • All Encephalitozoon species • Canine adenovirus 1
• All pathogenic Salmonella species • Canine adenovirus 2
product range addresses some truly • Anaplasma centrale • Canine coronavirus
unique challenges in the field. • Anaplasma marginale • Canine Distemper Virus
• Anaplasma phagocytophilum • Canine herpes virus
• Aspergillus • Canine influenza (H3N8)
• Aspergillus fumigatus • Canine Norovirus
• Aspiculuris tetraptera • Canine parainfluenza virus
• Atlantic salmon paramyxovirus • Capripoxvirus
• Avian adenovirus EDS76 Egg Drop Syndrome • Carnivore protoparvovirus 1
• Avian Infectious Bronchitis Virus (IBV) • Chicken anemia virus
• Avian Influenza A Virus Subtype H5 • Chlamydiaceae (all species)
• Avian Influenza A Virus Subtype H6 • Chlamydophila abortus
• Avian Influenza A Virus Subtype H7 • Chlamydophila felis
• Avian Influenza A Virus Subtype H9 • Chlamydophila pneumoniae
• Avian orthoreovirus • Chlamydophila psittaci
• Babesia bigemina • Classical swine fever virus
• Babesia bovis • Clostridium tetani
• Babesia caballi • Columbid Circovirus
• Babesia canis • Columbid herpesvirus 1
• Babesia divergens • Coxiella burnetii
• Babesia gibsoni • Crimean-Congo Haemorrhagic Fever Virus
• Bacillus anthracis • Cryptococcus neoformans
• Bartonella henselae • Cryptosporidium
• Batrachochytrium dendrobatidis • Cyclospora cayetanensis
• Beak and Feather Disease Virus • Cyprinid herpesvirus 3
• Betanodavirus • Dirofilaria immitis
• Bird flu • Dobrava-Belgrade virus
• Bluetongue Virus • Duck Hepatitis B Virus
• Bluetongue Virus 1 • Echinococcus granulosus
• Bordetella Bronchiseptica and Bordetella • Ehrlichia canis
Parapertussis • Encephalitozoon cuniculi
• Bovine adenovirus 3 • Encephalitozoon species
• Bovine adenovirus 5,6 and 8 • Enterocytozoon bieneusi
• Bovine ephemeral fever virus • Enterohemorrhagic Escherichia coli
• Bovine herpesvirus 1 • Enteroinvasive Escherichia coli
• Bovine Leukemia Virus • Enteropathogenic Escherichia coli
• Bovine parainfluenza virus 3 • Enterotoxigenic Escherichia coli
• Bovine Parvovirus • Epizootic haematopoietic necrosis virus
• Bovine Respiratory Corona Virus • Epizootic Hemorrhagic Disease Virus
• Bovine Respiratory Synctial Virus • Equid Herpesvirus 1
• Brachyspira hyodysenteriae • Equid Herpesvirus 2
• Brucella abortus • Equid Herpesvirus 3
• Brucella suis • Equid Herpesvirus 4
• Budgerigar Fledgling Disease Virus (avian • Equid Herpesvirus 5
polyomavirus) • Equine Arteritis Virus
• Burkholderia mallei • Equine infectious anemia virus
• Burkholderia pseudomallei • Equine Rhinovirus type 1
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Veterinary pathogen
kits
continued
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CEMO, K. pneumoniae and
P. aeruginosa test kits
Permission granted to be used by registered laboratories in the HBLB testing scheme.
• genesig real time PCR detection three kit bundle for Taylorella equigenitalis, Klebsiella
pneumoniae, and Pseudomonas aeruginosa
• genesig Standard real time PCR detection three kit bundle for Taylorella equigenitalis,
Klebsiella pneumoniae, and Pseudomonas aeruginosa
kits biothreat
• African Horse Sickness Virus
qPCR is the perfect tool for rapid • all pathogenic Salmonella species
• Alpha toxin producing Clostridium perfringens
detection of hazardous biological • Bacillus anthracis
agents like anthrax, cholera toxins etc. • Burkholderia mallei
• Burkholderia pseudomallei
• Chlamydophila psittaci
• Coxiella burnetii
• Cryptosporidium
• Escherichia coli 0157:H7
• Francisella tularensis
• H1N1 influenza
• Japanese Encephalitis Virus
• Marburg Virus
• Reston ebola virus
• Rift Valley Fever Virus
• Staphylococcus aureus
• Sudan Ebola Virus
• toxigenic subspecies of Vibrio cholerae
• Vaccinia virus
• West Nile Virus
• Western equine encephalomyelitis virus (WEEV)
• Yersinia enterocolitica
• Zaire ebola virus
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qPCR Somatic mutation
detection
Germline mutation
detection
test kits
genotyping • BRAF (V600E)
• JAK2 v617f
• EGFR-T790M
•
•
•
•
CSRP3-W4R
CYP2C19 codon 212
CYP2C19 codon 227
Cystic Fibrosis (CFTR)
quasa kits (Quantitative Allele
• Factor V Leiden
Specific Amplification) are developed • GABBR2-E421K
specifically for the detection of rare • GSTP1-A114V
mutations. quasa kits give specific • GSTP1-I105V
OnDemand Kits
Guaranteed High Quality Custom Kits Expertly Technical Information
Designed and Developed on Request Our standard assay formats include Double-Dye cleavage probe (e.g.
OnDemand Kits are Real-Time PCR assays developed on request to TaqMan® probes) or as primer only kits for use with SYBR ® Green
meet your specific requirements, giving you an assay of the highest chemistry.
quality, which specificially works for you.
Kit Contents
We offer precise qPCR assay development including primer/probe
design, PCR mix optimisation and assay development to ensure a • OnDemand Advanced Kit: Target primer & probe set, positive
very effective qPCR reaction. control, internal extraction control primer & probe set, internal
extraction control template, endogenous control primer & probe
This service allows for the combination of bioinformatic design and set, RNase/DNase free water, template preparation buffer,
development, in conjunction with laboratory testing and evaluation, Feasibility Report, Kit Data Sheet (incl. anchor nucleotide).
to provide personally designed primers to match your exact needs • OnDemand Easy Kit: Target primer & probe set, oasig®
and ensure high levels of sensitivity and specificity. Lyophilised qPCR Master Mix (DNA/RNA or RNA), oasig ®
resuspension buffer, positive control, internal extraction control,
Product Features RNase/DNase free water, template preparation buffer, 54x genesig
• Top quality assays with guaranteed results q16 reaction tubes, Feasibility Report, Kit Data Sheet (incl. anchor
• Designed by ‘in-house’ bioinformatical experts, not databases nucleotide).
• Scientifically validated in our laboratory
• Preliminary feasibility confirms if OnDemand assay is likely Visit our website for further information and FAQs, or please contact
possible us for additional information.
• An assay for almost any application
OnDemand Kits are sold for research use only and are not licensed
for diagnostic procedures.
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Master Mixes
• oasig® Lyophilised 2X qPCR Master Mixes:
• oasig® Lyophilised 2X qPCR Master Mix (oasig-standard-150)
• oasig® Lyophilised 2X OneStep RT-qPCR Master Mix (oasig-onestep-150)
The genesig Easy DNA/RNA Extraction Kit allows DNA/RNA and RNA extraction from virtually any sample type using magnetic bead
technology - it’s fast, and incredibly easy to perform.
• Extracts DNA/RNA and RNA with high yields <60 minutes
• Works with huge range of sample types
• Safe protocol with no phenolic chemicals
• No centrifuge or electrical equipment required - for use in the lab or in the field
The genesig easy DNA/RNA extraction protocol begins with a simple lysis step where cells and tissue are lysed to release their nucleic acid.
Then tiny magnetic particles are added to bind to RNA/DNA. When placed on to the genesig magnetic separator the particles are pulled to
the side of the tube making it easy to remove the unwanted supernatant with a pipette. Then a series of simple wash steps are performed
before the DNA/RNA is washed off the beads back in to solution, ready for analysis by real time PCR. Its fast, and incredibly easy to perform.
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NEW! genesig® q32
The genesig q32 Real-Time PCR Instrument provides fast results with genesig kits
Rapid heating and cooling makes the genesig q32 one of the fastest qPCR instruments on the market today. Assembled from
just a few building blocks, this robust qPCR instrument allows analysis of up to 32 samples in both tube or strip format. The
powerful genesig q32 software is easily installed from a USB flash drive onto any PC or Mac. The genesig q32 used together
with the dedicated genesig kits provide:
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genesig q32 - Cutting edge design and technology System specifications:
Assembled from just a few building blocks the robust genesig q32 system Dimensions: W 9.45 in x D 10.63 in x H 9.06 in
is easy to transport and install. Up to 32 samples can be run in 0.1 ml tubes (W 24 cm x D 27 cm x H 23 cm)
Weight 15.43 lbs (7 kg)
or 8-strip format. Fast heating and cooling is achieved by utilizing robust Operating noise <40 dB(A)
Peltier elements, whilst assay performance is supported further by a heated Electrical Voltage: 100 - 240 V (+ 10%)
lid design. Excitation is provided by high intensity LEDs filtered to provide Frequency: 50 - 60 Hz (+ 10%)
light at 500nm that is capable of exciting all fluorophores commonly used Power: 170 W
in qPCR. A prism is used to generate spectra from fluorescent emissions. Number of reactions 32
Reaction vessels 0.1ml tube and/or 8-tube strips
These spectra are imaged using a CMOS camera. Reaction volume 10 - 100 µl (20 µl recommended)
Temperature cycling Peltier-based heating & cooling from +40
genesig q32 software system to +99°C
The genesig q32 software is delivered and easily installed from a USB flash Heating rate: 4°C/s & Cooling rate: 3°C/s
drive. When installed in a network, the software can control several q32 Temperature uniformity +/- 0.05°C (SD)
instruments connected via LAN. The powerful and easy to use software Temperature accuracy +/- 0.25°C
Run time <60 min
provides the following features: Fluorescence excitation 500 nm nm (blue LED)
• Quick start using templates for all genesig kit applications Fluorescence detection 120 optical channels from 510 to 750 nm
(CMOS camera)
• Straightforward setup and editing of sample and target information Factory-calibrated dyes FAM; VIC / HEX
• Automated analysis modules for absolute and relative quantification, (at shipment)
melting curve analysis and endpoint genotyping Applications Relative and absolute quantification
• Analysis of full spectral data analysis
Melting peak analysis
• Compatible with Windows, Mac & Linux Systems
• Instrument start from a USB flash drive, using preprogrammed settings Sensitivity Endpoint genotyping analysis
1.1 fold discrimination
• Data export functions 9-log dynamic range
qPCRGPD-1219
Single copy detection
genesig Real-Time PCR Kit compatible Connection options LAN
Direct connection to computer (RJ45)
genesig Real-Time PCR Kits are suitable for use on the genesig q32 PC-free (USB stick/flash drive controlled)
instrument. The genesig Kit range includes over 550 kits for human, vet, and Software Genesig Q32 Software for open and
food pathogens, as well as food speciation testing. All genesig Kits include a close platform kits
positive control and resuspension buffers. The kits come freeze-dried so that
it can be shipped at room temperature for fast delivery to anywhere in the
World.
Automated run templates available for:
• genesig Easy Real-Time PCR Kits
• genesig Advanced Real-Time PCR Kits
• genesig Standard Real-Time PCR Kits
• snpsig Real-Time PCR Kits
• quasa Real-Time PCR Kits
• GMO Real-Time PCR Kits
MD606750 FM559592
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