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KEY WORDS
Bacterial requirements for growth include oxygen, (or its absence) nutrients, optimal
temperature and pH.
Oxygen Requirements: Obligate aerobes must grow in the presence of oxygen; they can’t
carry out fermentation. Obligate anaerobes do not carry out oxidative phosphorylation.
Furthermore, they are killed by oxygen; they lack certain enzymes (e.g. catalase [decomposes
+
hydrogen peroxide, H2O2, to H2O and O2], peroxidase (H2O2+ NADH + H converted to NAD
and 2H2O), superoxide dismutase (superoxide [O2-] to H2O2) which detoxify both H2O2and
oxygen free radicals (superoxide) produced as side-products during metabolism in the presence
of oxygen. Aerotolerant anaerobes are bacteria that respire anaerobically, but can survive in the
presence of oxygen. Facultative anaerobes can perform both fermentation and aerobic
respiration. In the presence of oxygen, anaerobic respiration is generally shut down and these
organisms respire aerobically. Microaerophilic bacteria grow well in low concentrations of
oxygen, but are killed by higher concentrations.
Nutrient Requirements: include sources of organic carbon, N, P, S and metal ions (e.g.
iron). Bacteria secrete small molecules that bind iron (siderophores, e.g. enterobactin and
mycobactin). Siderophores (with bound iron) are then internalized via receptors by the bacterial
cell. The human host also has iron transport proteins (e.g. transferrin). Thus bacteria that
ineffectively compete with the host for iron are poor pathogens.
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near to the boiling point of water. Those that grow best at the middle of this range are referred to
as mesophiles; which includes all human pathogens and opportunists. Those having lower and
higher temperature optima for growth are respectively known as psychrophiles and thermophiles.
pH: Many bacteria grow best at neutral pH; however certain bacteria can survive and
even grow in quite acid or alkaline conditions.
Measuring bacterial mass in liquid cultures of bacteria: Common methods include: (a)
Turbidity (how cloudy is a liquid culture of bacterial -a measure of total bacteria [live and
dead]). This is usually quantitated with a spectrophotometer or (b) Number of viable bacteria in a
culture - usually assessed by counting the number of colonies that grow after streaking a known
volume on a plate (“plate counting” of colony forming units). In either case plotting the log of
turbidity or number of living cells versus time is referred to as the growth curve. In the lag
phase, since bacteria are adapting to the media, there is little or no growth. During the log phase
– growth is fast (logarithmic). In the stationary phase the bacteria stop growing. Finally, as the
nutrient supply is exhausted they start to die (death phase). In many cases (but not always) the
bacteria autolyse (during the death phase) and the turbidity decreases. The generation time is
defined as the time required for bacterial mass to double.
Lag Lag
TIME TIME
METABOLISM OF SUGARS
Step 1.
Glycolysis (Embden Meyerhof Parnas Pathway) is the most common pathway in bacteria for
sugar catabolism (also found in most animal and plant cells). A series of enzymatic reactions
convert sugars into pyruvate, generating ATP (adenosine triphosphate) and NADH
(nicotinamide adenine dinucleotide). Chemical energy needed for biosynthetic purposes are
stored in the newly formed compounds (ATP and NADH).
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NAD NADH
Glucose Pyruvate
(C6) ADP ATP (C3)*
*
refers to number of carbons in molecule
There are alternatives, for catabolizing sugars, to produce stored energy within ATP
including the pentose phosphate pathway (hexose monophosphate shunt) that is found in most
animal and plant cells. NADPH is generated using this pathway. A third pathway (the Entner
Doudoroff pathway) is found in a few species of bacteria and is not seen in animals.
Step 2 –either A or B
A. Anaerobic Respiration
Anaerobic respiration includes glycolysis and fermentation. During the latter stages of
this process NADH (generated during glycolysis) is converted back to NAD by losing a
hydrogen molecule. The hydrogen is added to pyruvate and depending on the bacterial species a
variety of metabolic end-products can be produced.
NADH NAD
Pyruvate Short chain alcohols or
(C3) fatty acids (e.g. lactic acid or ethanol)
(C2-C4)
B. Aerobic Respiration/Catabolism
Aerobic respiration involves glycolysis and the tricarboxylic acid cycle (Krebs cycle).
Pyruvate is fully broken down to CO2 and in the process NAD converted to NADH. Thus in
aerobic fermentation NADH is generated from two sources (glycolysis and the Krebs cycle).
Oxidative phosphorylation converts excess NADH back to NAD and in the process produces
more ATP (stored energy). Ubiquinones and cytochromes are components of the electron
transport chain involved in this latter process. Conversion of oxygen to water is the final step in
the process.
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Catabolism/Krebs Cycle
NAD NADH
Pyruvate 3 CO2
(C3) (C1)
Oxidative phosphorylation
NADH NAD
O2 H2O
ADP ATP
C2 Isocitrate X
C
Acetate -CO2 NADH
Citrate C6
-CO2
+ Alpha-keto
Oxaloacetate C4 glutarate
Pyruvate -CO2 NADH
Malate x
Succinate C
Fumarate
Thus if fatty acids are the sole carbon source then no Krebs Cycle intermediates can be removed
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without shutting it down
+ C2
C4 C6
Instead bacteria utilize the glyoxylate cycle (a modified Krebs Cycle). The 2 enzymatic
steps in which CO2 molecules are removed from intermediates of the cycle are by-passed.
Instead a C6 intermediate is converted to two C4 compounds (both components of the cycle).
Thus for every acetyl group (from fatty acids) an additional cycle intermediate can be produced.
The glyoxylate pathway is not generally found in animal cells since pre-formed fatty acids,
present in food, are utilized.
C6 C4
+
+ C2
C2 C4
Take home message the Krebs Cycle functions in a biosynthetic and energy producing fashion.
However, if intermediates are to be removed for use in other metabolic pathways they must be
replenished. The replenishment process for utilization of sugars and fatty acids is different.