TSS & VSS

Weight of empty aluminum dish, = 1604 mg

Volume of water sample pass through the filter paper = 25 mL
Weight of aluminum dish + filter paper (A) = 1670 mg
Weight of aluminum dish + filter paper (after filtration), = 1837 mg
Weight of aluminum dish + filter paper (after drying at 105 oC), (B) = 1678 mg
Weight of aluminum dish + filter paper (after ignotation at 550 oC), (C) = 1602 mg

Calculation:

( B−A ) x 1000
TSS (mg/L) =
sample volume∈mL
( 1678−16 70 ) x 1000
=
25
= 320 mg/L

( B−C ) x 1000
VSS (mg/L) =
sample volume∈mL
( 1678−1602 ) x 1000
=
25
= 3040 mg/L
pH, AN & COD

Parameters Reading 1 Reading 2 Reading 3

pH 6.738 6.708 6.740
AN (mg/L) 0.50 0.51 0.50
COD (mg/L) 24.1 24.2 24.0

Calculation:

6.738+6.708+6.740
pH =
3
= 6.729

0.50+0.51+0.50
AN =
3
= 0.503 mg/L

( mgO 2∈final volume )( 1000 )

COD =
sample∈mL
Since results in mg/L;
24.1+ 24.2+ 24.0
COD =
3
= 24.1 mg/L
DO
Volume of water sample = 100 mL
Volume of Winkler bottle = 300 mL
DO at Day 1 = 7 mg/L
DO at Day 5 = 5 mg/L

Calculation:

( DO 1 – DO 5 )−( S ) Vs
𝐵𝑂𝐷5 (𝑚𝑔/𝐿) =
P
where:
D1 = DO of diluted sample immediately after preparation, mg/L
D5 = DO of diluted sample after 5 days’ incubation, mg/L
S = Oxygen uptake of seed, Δ DO/mL seed suspension added per bottle
(¶ 6d) (S = 0 if samples are not seeded)
Vs = Volume of seed in the respective test bottle, mL
P = Decimal volumetric fraction of sample used

volume of water sample

P=
volume of BOD bottle

Note: Only consider dilutions where: (1) depletion is ≥ 2.0 mg/L, and
(2) final DO ≥ 1.0 mg/L.

100
P =
300
1
=
3

( 7−5 )−0
BOD5 (mg/L) = 1
3
= 6 mg/L