PRACTICAL 8

DETERMINATION OF VITAMIN C ( ASCORBIC ACID )

Information on preparation of sample :

Weight of pineapple being used = 298.18 g

Final volume of homogenous slurry (sample + 6% HPO3) = 500 ml

Aliquot of homogenous slurry used for dilution to 100ml with 3% HPO3 = 30 ml

Volume of filtrate of supernatant used for titration = 10 ml

Table 8.1: Titration of ascorbic acid standard

Ascorbic acid standard

Trial #1 Trial #2 Trial #3

Initial burette reading (ml) 0.00 8.30 16.50

Final burette reading (ml) 8.30 16.50 24.70

Volume used (ml) (final-

initial)

Average (ml)

Table 8.2: Titration of unknown concentration of vitamin C in sample

Sample: Pineapple

Trial #1 Trial #2 Trial #3

Initial burette reading (ml) 0.00 6.20 12.30

Final burette reading (ml) 6.20 12.30 18.30

Volume used (ml) (final-

initial)
Table 8.3: Amount of ascorbic acid in pineapple

Sample: Pineapple

Trial #1 Trial #2 Trial #3

Amount of ascorbic acid in

pineapple
(mg /100 g sample)

Average amount of ascorbic acid

in pineapple
(mg/ 100 g sample)