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Encircle the correct option. CHAPTER NO: 03 Time allowed: hours
* * *
By: Rajesh Kumar (J .S .T ) ENZYME Class: XI th Date: 22/03/2020
NOTE: Cutting & over writing is not allowed.
1. Oxidoreductases include: (a) Catalases and Mutases (b) Oxygenases and Dehydrogenases (c) Dehydrogenases and Ligases
(d) Aminotransferases and Racemases (e) Epimerases and Oxidases
2. Michaelis constant of enzyme (Km) is: (a) The substrate concentration which gives maximal velocity (b) The substrate
concentration which gives 1/2 the maximal velocity (c) 1/2 the substrate concentration which gives maximal velocity
(d) The enzyme concentration which gives maximal velocity (e) The enzyme concentration which gives 1/2 the maximal
velocity
3. A competitive inhibitor: (a) Increases the Km of the enzyme (b) Decreases the Vmax of the enzyme (c) Increases the Vmax
of the enzyme (d) Decreases the Km of the enzyme (e) Increases both Km and Vmax of the enzyme
4. The non-protein part of an enzyme is called:
(a) Apoenzyme (b) Holoenzyme (c) Allosteric Enzyme (d) Isoenzyme (e) Coenzyme
5. The major 6 classes of enzymes include: (a) Transferases and Oxygenases (b) Isomerases and Lyases (c) Hydrolases and
Epimerases (d) Oxidoreductases and Catalases (e) Transaminases and Transferases
6. Enzymes: (a) Increase the energy barrier for a reaction (b) Are recovered chemically altered after a complete reaction
(c) Increase the equilibrium constant of the reaction (d) Activity is subject to regulation (c) Are exclusively proteins
7. Coenzymes: (a) The non-protein part of enzymes (b) Needed for enzyme activity (c) Usually vitamin derivatives
(d) Termed prosthetic group if bonded tight to their enzymes (e) all of the above are correct
8. At Vmax of an enzyme catalyzed reaction: (a) Further increase in substrate concentration increases the rate of the
reaction (b) All the enzyme molecules are combined with substrate (c) The substrate concentration that produces maximal
velocity is termed Km (d) Half the enzymes are combined with substrate (e) Km is maximum
9. In case of non-competitive inhibition of enzymes: (a) Vmax cannot be reached (b) There is no significant alteration of the
active site (c) Inhibitor is structurally similar to the substrate (d) Inhibitor can be removed by increasing substrate
concentration
(e) Vmax is increased
10. Isoenzymes: (a) Are factors required for enzyme activity (b) Are functional plasma enzymes (c) Show similar responses to
inhibitor molecules (d) Are important tools in the diagnosis & prognosis of disease (e) Catalyze oxidation-reduction
reactions
11. Hydrolases enzymes include: (a) Racemases and oxygenases (b) Isomerases and lyases (c) Peptidases and lipases
(d) Oxidoreductases and catalases (e) Transaminases and hydralases
12. Which of the following statements is true of enzyme catalysts?
(a) To be effective they must be present at the same concentration as their substrate
(b) They can increase the equilibrium constant for a given reaction by a thousand-fold or more
(c) They lower the activation energy for conversion of substrate to product
(d) Their catalytic activity is independent of pH
(e) They are generally equally active on D and L isomers of a given substrate
13. Allosteric effector influences the enzyme activity by:
(a) Covalently modifying the enzyme (b) Binding to the substrate and altering its conformation
(c) Competing for catalytic site with substrate (d) Changing the specificity of the enzyme for its substrate (e) Binding to a site
on the enzyme distinct from catalytic site
14. The following enzyme complex requires both thiamine and lipoic acid: (a) Malate dehydrogenase (b) Alpha-ketoglutarate
dehydrogenase (c) Fumarase (d) Isocitrate dehydrogenase (e) Succinate dehydrogenase
15. Sterioisomers: (a) Diversify physical and chemical properties of molecules (b) Have the same spatial configuration
(c) Exhibit enzyme specifity (d) Include epimers, anomers and enantiomers (e) all A, C and D are correct
16. In an enzyme catalyzed reaction: (a) Enzymes increase the energy barrier for the reaction (b) Enzymes form a perminant
complex with the substrate (c) After the reaction, enzymes are recovered unchanged (d) V-max is reached in the presence of
 a non-competitive inhibitor (e) The equilibrium constant is decreased
17. Isomerases: (a) Racemases and mutases (b) Phosphatases and hydratases (c) Lyases and oxidases (d) Transaminases and
kinases (e) Hydratases and glucosidases
18. Subclasses of oxidoreductases include: (a) Kinases and oxygenases (b) Reductases and dehydrogenases (c) Racemases and
oxidases (d) Catalases and aminotransferases (e) Peroxidases and anomerase
19. The active site of an enzyme: (a) is rich with hydrophobic amino acid side chains (b) Binds allosteric effectors
(c) is similar in shape to the substrate (d) Occupies most of the enzyme molecule (e) N.O.T
20. Coenzymes: (a) Are small organic molecules needed for enzyme activity (b) Are associated with apoproteins
(c) Are vitamin derivatives (d) are called prosthetic groups if they are tightly bound to the enzyme (e) A.O.T
21. In competitive inhibition of an enzyme:
(a) Km and Vmax are decreased (b) Inhibitor is structurally similar to the substrate (c) Inhibition cannot be reversed by
increasing substrate concentration (d) Vmax is decreased (e) The inhibitor binds to the regulatory site on the enzyme
22. In non-competitive inhibition of enzyme activity:
(a) Both Vmax and Km are increased (b) Inhibitor has no structural resemblance to the substrate (c) There is a significant
alteration of the active site (d) Vmax is decreased (e) A is the only wrong answer
23. The following statements describe Allosteric enzymes: (a) Effectors may enhance or inhibit substrate binding (b) Binding
of the substrate to the Allosteric site displaces the effectors (c) They are always multimeric proteins (d) The regulatory site
is
identical to the active site (e) Binding of substrate to the active site releases allosteric effector
24. The following statements about regulation of enzyme activity are correct:
(a) Phosphorylation/ dephosphorylation is the common covalent modification (b) Regulatory enzymes usually catalyze
reversible reactions (c) Allosteric inhibitors are usually the end products (d) Increase in the enzyme level has no effect
(e) Irreversible inhibition is physiological type of regulation
25. Phosphorylation/ dephosphorylation of enzymes: (a) Occurs on specific serine and threonine residues (b) Is a covalent
type
of modification (c) Is a reversible type of regulation (d) is achieved by the activities of protein kinases and phosphatases
(e) A.O.T
26. The enzyme for starch digestion is: (a) Salivary amylase (b) Pancreatic amylase (c) Lysosomal glucosidase (d) Both A and B
(e) All of the above is correct
27. Hydrolases include: (a) Catalases and mutases (b) Oxygenases and dehydrogenases (c) Esterases and glucosidases
(d) Aminotransferases and racemases (e) Epimerases and oxidases
28. The following enzymes are absent in muscles but are present in the liver:
(a) Pyruvate carboxylase and citrate synthase (b) Glucose-6-phosphatase and glycerol kinase
(c) Lactate dehydrogenase and isocitrate dehydrogenase (d) Pyruvate dehydrogenase and beta-ketothiolase
(e) Glycogen synthase and 3-HMG CoA synthase
29. Which of the following statements is true of enzyme catalysts?
(a) To be effective, they must be present at the same concentration as their substrate
(b) They can increase the equilibrium constant for a given reaction by a thousand-fold or more
(c) They lower the activation energy for conversion of substrate to product
(d) Their catalytic activity is independent of pH (e) they are generally equally active on D and L isomers of a given substrate
30. Isoenzymes: (a) Are differentiated by dialysis (b) Show the same electrophoretic mobility (c) Include lactate
dehydrogenase
(d) Are used as diagnostic tools (e) Both C and D are correct
31. The pancreatic enzyme not involved in protein digestion is:
(a) Carboxypeptidase (b) Trypsin (c) Lipase (d) Elastase (e) Chymotrypsin
32. Restriction enzymes:
(a) Recognize specific nucleotide sequences in DNA (b) Cut both strands of DNA (c) Often produce single stranded tails
(d) Do all of the above (e) do none of the above?
33. Enzymes involved in hydrolysis:
(a) Are called decarboxylases (b) Use folic acid as a coenzyme (c) Include dehydrogenases (d) Include peptidases (e) Always
require coenzyme
34. Enzymes and pH: (a) All enzymes have one optimum pH (b) Enzymes in the stomach require alkaline pH (c) All enzymes
in eukaryotic cells require the same pH (d) The optimum pH is the one at which the enzyme has maximum activity (e) The pH
 is the same in the lysosome and plasma
35. Isomerases:
(a) Catalyze oxidation-reduction reactions (b) Do not change the structural formula of the substrate
(c) Include transaminase (d) Use water to break covalent bonds (e) Require ATP to form covalent bonds
36. The following vitamin work as coenzymes with the corresponding enzyme: (a) Biotin and carboxylases (b) Cobalamin
with
isomerases (c) Retinol with lyases (d) Riboflavin with hydrolases (e) Thiamin and isomerases
37. Who is the father of enzymology? (a) Anton von Leeuwenhoek (b) Edward Howel (c) JB Sumner (d) John Northrop
38. Which of the following statement/s is/are correct?
(a) Enzyme is a biocatalyst (b) Enzyme increases the rate of reaction
(c) Enzyme decreases the activation energy of the reaction (d) All the above
39. Nature of the enzyme is: (a) Protein (b) Lipid (c) Carbohydrate (d) None
40. An enzyme was crystallized for the first time by: (a) John Northrop (b) Edward Buchner (c) J.B. Sumner (d) None
41. Which of the following is correct?
(a) Apoenzyme + Cofactor = Holoenzyme (b) Apoenzyme - Cofactor = Holoenzyme
(c) Apoenzyme =Cofactor - Holoenzyme (d) None
42. Enzymes are classified into _____ types: (a) Two (b) Three (c) Five (d) Six
43. Competitive inhibitor of an enzyme is: (a) Structural analogue of enzyme (b) Functional analogue of enzyme
(c) Functional analogue of substrate (d) Structural analogue of substrate
44. Majority of the enzymes are inactive at: (a) At 25ºC (b) At 15ºC (c) Between 30-40ºC (d) Above 70ºC
45. Induced fit theory for the formation of Enzyme-Substrate complex was proposed by:
(a) Koshland (b) Charles Leibeg (c) Buchner (d) Fischer
46. SI Unit for enzyme activity is: (a) Bel (b) mho (c) Hertz (d) Katal
47. Which of the following is the reason for Gout disease? (a) Deficiency of Xanthine Oxidase enzyme (b) Over production of
Xanthine Oxidase (c). Deficiency of ß-galactosidase enzyme (d) Deficiency of Lipase enzyme
48. Which of these enzymes is related to molecular biology? (a) DNA Ligase (b) DNA topoisomerase (c) Polymerase (d) A.O.T
49. Deficiency of the enzyme ß-glucerebrosidase results in:
(a) Gauchers Disease (b) Faber’s disease (c) Krabbe’s disease (d) Lesh-Nyhan Syndrome
50. Enzyme that differ in amino acid sequence but catalyze the same reaction are_______.
(a) Co-factors (b) co-enzymes (c) Apo enzymes (d) Isoenzymes
51. The temperature at which the maximum activity of the enzyme occurs is:
(a) Optimum temperature (b) Maximum Temperature (c) Minimum Temperature (d) None
52. The class of enzymes that catalyzes synthetic reactions where two molecules are joined together and ATP is used is:
(a) Hydrolases (b) Lyases (c) Ligases (d) Isomerases
53. Non-protein organic part of the enzyme is__________. (a) Co-factor (b) Co-enzyme (c) Apo enzyme (d) Isoenzyme
54 Pepsin and urease are examples for which class of enzymes: (a) Hydrolases (b) Ligases (c) Oxidoreductases (d) Lyases
55. Which of the following enzymes is elevated in alcoholism:
(a) Hepatitis (b) Serum Glutamate Pyruvate Trasmittase (c) Acid Phosphatase (d) glutamyl transpeptidase
56. Which one of the following statements is true with reference to enzymes?
(a) Apoenzyme = Holoenzyme + Coenzyme (b) Holoenzyme = Apoenzyme + Coenzyme
(c) Coenzyme = Apoenzyme + Holoenzyme (d) Holoenzyme = Coenzyme – Apoenzyme
57. Restriction endonuclease is employed for:
(a) Cutting RNA (b) Cutting single stranded DNA (c) Cutting double stranded DNA (d) Joining strands of DNA
58. None protein part of enzyme is called: (a) Ketone group (b) Prosthetic group (c) Apoenzyme (d) Transferase enzyme
59. Enzymes functional inside the cell are called: (a) Endoenzyme (b) Exoenzyme (c) Apoenzyme (d) Isoenzyme
60. The polypeptide or protein part of the enzyme is called: (a) Apoenzyme (b) Holoenzyme (c) Prosthetic group (d) Zymogen
61. Turn over number of an enzyme is dependent upon:
(a) Size of enzyme (b) Molecular weight of enzyme (c) Active sites (d) Concentration of substrate
62. Which of the following is produced with the combination of Apoenzyme & coenzyme?
(a) Holoenzyme (b) Enzyme substrate complex (c) Prosthetic group (d) Enzyme product complex
63. In which of the following organisms enzymes are absent: (a) Fungi (b) Viruses (c) Algae (d) Bacteria
64. NADP+ is: (a) Enzyme activator (b) Ion carrier (c) Hydrogen acceptor (d) Electron acceptor
65. Most of the members of vitamin B complex act as: (a) Cofactor (b) Coenzyme (c) Prosthetic group (d) Apoenzyme
66. An enzyme that joins the ends of two strands of nucleic acid is: (a) Polymerase (b) Ligase (c) Synthetase (d) Helicase
67. Most of enzymes are highly active at: (a) 25℃ (b) 37℃ (c) 20℃ (d) 32℃
68. Key lock theory was produced by: (a) Friendrich (b) Robert hook (c) Fischer (d) Koshland
69. The enzymes which active within the cell are called: (a) Endoenzyme (b) Ectoenzyme (c) Exoenzyme (d) N.O.T
70. Each enzymes has groove of a specific shapes which is called: (a) Competitive site (b) Inactive site (c) Active site (d) N.O.T
71. Conjugated enzymes also called: (a) Holoenzymes (b) Apoenzyme (c) Activators (d) N.O.T
72. Enzymes exist in the cells as: (a) Solution (b) Crystals (c) Solids (d) Colloids
73. Enzymes are sensitive to: (a) Cold (b) Cell wall (c) Heat (d) Pressure
74. A protein having both structural & enzymatic traits is: (a) Collagen (b) Trypsin (c) Myosin (d) Actin
75. Ribozyme is: (a) RNA without phosphate (b) RNA with sugar (c) RNA with enzyme activity (d) RNA with extra phosphate
76. Enzyme catalyzing rearrangement of atomic groupings without altering molecular weight or number of atoms is:
(a) Ligase (b) Isomerase (c) Oxidoreductase (d) Hydrolase
77. The enzyme which hydrolyses starch to maltose is: (a) Protease (b) Amylase (c) Lactase (d) Maltase
78. Enzymes are basically made up of: (a) Fats (b) Proteins (c) Nucleic acids (d) Vitamins
79. Enzymes are polymers of: (a) Hexose sugar (b) Amino acids (c) Fatty acids (d) Inorganic phosphate
80. Prosthetic group is: (a) Organic (b) Thermostable (c) Losely attached to the enzyme (d) Called Apoenzyme
81. The catalytic activity of an enzyme is restricted ti its small portion called:
(a) Active site (b) Passive site (c) Allosteric site (d) A.O.T
82. An activated enzyme made up of polypeptide chain & co-factor is: (a) Coenzyme (b) Substrate (c) Apoenzyme (d)
Holoenzyme
83. Enzymes are largely in their chemical nature. (a) Lipids (b) Steroids (c) Proteins (d) A.O.T
84. The non-protein part of an enzyme is known as: (a) Holoenzyme (b) Vitamin (c) Apoenzyme (d) Prosthetic group
85. Many enzymes are secreted in inactive form to protect: (a) Cell membrane (b) Mitochondria (c) Cell protein (d) Cell DNA
86. The first enzyme to be purified & crystallized was: (a) Urease (b) Diastase (c) Insulin (d) Zymase
87. In a cell, digestive enzymes mostly occur in: (a) Mitochondria (b) Lysosomes (c) Ribosomes (d) Plastids
88. Enzymes having slightly different molecules structure but performing identical activity are:
(a) Apoenzymes (b) Isoenzymes (c) Holoenzymes (d) Coenzymes
89. Enzymatic activity is:
(a) Increased due to water (b) Decreased due to water (c) Not effected due to water (d) Inhibited due to water
90. Enzymes contain another group with protein is called
(a) Conjugated enzymes (b) Simple enzymes (c) Non conjugated enzymes (d) N.O.T
91. Which molecules binds to the active site of an enzyme:
(a) Allosteric inhibitor (b) Allosteric activator (c) Non-competitive inhibitor (d) Competitive inhibitor
92. Protein part of an enzyme is called: (a) Apoenzyme (b) Ketone group (c) Prosthetic group (d) Transferase enzyme
93. The active site of an enzyme is formed by a few of the enzyme:
(a) R-groups of amino acids (b) NH2 groups of amino acids (c) –COOH groups of amino acids (d) Exposed disulphide acids
94. Milk drinking babies have an additional enzyme called: (a) Renin (b) Amylase (c) Lipase (d) N.OT
95. Which enzyme present in stomach curdles the milk? (a) Rennin (b) Trypsin (c) Pepsin (d) Lipase
96. A temperature beyond optimum: (a) Can affect the shape of an enzyme (b) Lowers the energy of activation
(c) Makes cells less suspectible to disease (d) both a & c
97. Nucleic acid which also serves as enzymes are: (a) Nucleoprotein (b) Ribozyme (c) (Ribosome d) Co-enzyme
98. The activity of almost every enzyme in a cell is regulated by:
(a) Feed-back inhibition (b) Positive-feedback (c) Negative-feedback (d) Feed-back control
99. Magnessium (Mg+2) is an inorganic activator for the enzyme: (a) Enolase (b) Phosphatase (c) Hexokinase (d) A.O.T
100. If the prosthetic group is an organic molecule, it is called: (a) Inhibitors (b) Activators (c) Co-enzymes (d) N.O.T
101. Inorganic substances which increases the activity of an enzyme are called:
(a) Inhibitors (b) Activators (c) Co-enzymes (d) N.O.T
102. Substances which decrease the activity of an enzyme are called: (a) Inhibitors (b) Activators (c) Co-enzymes (d) N.O.T
103. Pepsin of stomach has an optimum pH of 1.4. It is inactive in solution.
(a) Neutral (b) Alkaline (c) Acidic (d) Both a & b
104. At a very high concentration the substrates exerts a retarding effect upon enzyme action. This may be due to reasons:
(a) Higher quantity of substrate than enzyme (b) Accumulation of end product in high quantity (c) both a & b (d) None
105. Some co-enzymes of oxidation & reduction processes are: (a) NAD (b) NADP (c) FMN (d) ATP (e) A.O.T
106. Enzymes activities can be inhibited by certain factors called inhibitors e.g:
(a) Substrate concentration (b) Enzyme concentration (c) pH (d) A.O.T
107. Most of the enzymes are the in nature. (a) Globular proteins (b) Fibrous proteins (c) Both a & b (d) N.O.T
108. Chemical transformation requires that certain covalent bonds be broken within the reactants. To do so the reactants must
contain sufficient kinetic energy (energy of motion) to overcome a barrier called:
(a) Energy of activation (b) Activation energy (c) Additional energy (d) A.O.T
109. The figure below shows the conversion of a substrate into product by an enzyme. In which one of the four option (1-4) the
components of reaction labelled as A, B, & D are identified correctly?

110. Symbolic representation which of the following model of enzyme action.

E (a) Lock & Key model (b) Induced Fit model (c) Both (d) N.O.T

111. Symbolic representation which of the following model of enzyme action.

(a) Lock & Key model (b) Induced Fit model (c) Both (d) N.O.T

112. Following figure shows which type of enzymatic action inhibition.

(a) Enzymatic reaction proceeds normally (b) Competitive inhibitor (c) Non-competitive inhibitor (d) Feedback inhibitor
113. Following figure shows which type of enzymatic action inhibition.
 (a) Enzymatic reaction proceeds normally (b) Competitive inhibitor (c) Non-competitive inhibitor (d) Feedback inhibitor
114. Following figure shows which type of enzymatic action inhibition.

(a) Enzymatic reaction proceeds normally (b) Competitive inhibitor (c) Non-competitive inhibitor (d) Feedback inhibitor