Cytation™ Cell Imaging Multi-Mode Reader

Software Compatibility

Updates to Cytation 3 for Gen5 Upgrade

Compatibility
NOTE
• Cytation 3 customers who wish to upgrade from Gen5 version 2.04 will
need to do the following:
o Contact customercare@biotek.com for a new Gen5 serial number
o Update Cytation 3 basecode as described in section A
o Update the Firewire driver as described in section B

• Cytation 3 customers who wish to upgrade from Gen5 version 2.05 must
update the Firewire Driver as described in section B

A. Instrument Base Code Download

The latest version of Cytation 3 base code is available from the Customer Resource Center under
Cytation 3 > Instrument base code. Be sure to unzip any software to obtain the .bin extension before
attempting any downloads.

IMPORTANT
The filter, Imaging Filter Cubes, Objective and Dispenser Cal values may
need to be updated after base code download. You may want to record
the info prior to beginning the update process. Alternatively, you can
check the installed components for the information.

1. Save the base code file to your desktop

Base code file name cytation3.bin

 Cytation™ Cell Imaging Multi-Mode Reader
Software Compatibility

2. From the Gen5 main screen, select

System,
Instrument Configuration,
View/Modify,
Test Comm.

If the reader is communicating, select OK

and proceed to next step.

3. To enable Gen5 Test Tools

type CTRL + SHIFT + X

select Setup…

4. Backup the instrument calibration data

Select the Auto-Calibration tab then

Select Save Complete Configuration

 Cytation™ Cell Imaging Multi-Mode Reader
Software Compatibility

5. Select the Miscellaneous tab

Select Download Basecode…

6. Use the … button to navigate to Cytation

3.bin previously saved on your desktop

Select Start Download…

7. Verify the part number and version

before selecting YES

It is normal to hear the instrument fan

turn off.

8. The instrument will initialize and prepare

for download

Select OK when you hear the beep

(if you do not hear a beep, select OK after

30 seconds)
 Cytation™ Cell Imaging Multi-Mode Reader
Software Compatibility

9. You should see this window

The number of Bytes Transferred should
be increasing

10. After the download completes, the

instrument will reset and begin a system
test

Once the system test completes, the

instrument will beep and the LED on the
carrier eject button should be green

11. Select the Determine Capabilities button

in the base code window to complete the
update

If you are upgrading to version 1.14, you

must calibrate the objectives.

B. Firewire Driver Installation (Desktop or Laptop)

1. Install Gen5 if not already installed.

Gen5 will save the necessary driver installation files to the following or similar path during
installation:
C:\program Files\BioTek\Gen5.2.xx\Firewire Drivers
Gen5 2.04 or higher.
 Cytation™ Cell Imaging Multi-Mode Reader
Software Compatibility

2. Navigate to the FireWire Drivers folder

C:\program Files\BioTek\Gen5.2.xx\Firewire Drivers (or similar)

Select the folder that reflects the Windows operating system installed on the computer:
Windows_32 or Windows_64. In Windows 7 this information is found under Control Panel,
System.

3. Run the file named “InstallPGRDriver”

This is a MS-DOS batch file.

In Windows XP this file can be executed by selecting on it. If you have Window 7 or higher, right-
select on the file and select Run as Administrator.

4. The drivers will install.

Wait to see a “successful” status displayed near
the bottom of the DOS window. Press any key
to close the window.
If you do not see the word “successful” the
drivers were not installed. Make sure you have
Windows administrator rights and try again.

Laptop Version

5. Reboot the computer to complete the installation

6. Connect the FireWire cable to the Cytation 3

and power up the instrument
 Cytation™ Cell Imaging Multi-Mode Reader
Software Compatibility

7. Connect the Fire Wire cable to the Cytation 3

camera and power on the instrument. You
should see the colored LED on the back of the
camera where the cable is connected will
eventually turn green. This will indicate the
installation was successful and the camera and
computer established communication.

• Red - Power from PC to Camera

• Red/Green - Camera has completed power
up sequence and is in standby mode
• Green - Communicating with computer

NOTE: Gen5 must FIRST be connected to the

instrument and communicating before
communication can be established with the
camera.