{In the name of Allah the merciful the compassionate }

Biochemistry
Questions
&
Answers
A-Naeem Al-sawaie
Jordanian University of Science and Technology (JUST)
Medicine & surgery –MD
1-Free energy change(DG) – the energy change for a reaction depends only on initial and final states
and is independent of the path taken .

A-true

B- false

ANSWER: A

2-Free energy change(DG)- the overall energy change for a reaction could be calculated by summing the
energy changes for a series of separate reactions that could convert the reactants to the products

A- True
B- False

ANSWER: A

3-Free energy change (DG)- the rate of a reaction can be determined from the energy change

A-true

B –false

ANSWER: B

4-Free energy change(DG) – the energy change is a function of the concentration of the products and
reactants at start

A – true

B-false

ANSWER: B

ADDITIONAL QUASTION :The standard state is of solution is taken as

A-1 molar concentration

B- 297 K

C- 10 atm

D- all of them are correct

ANSWER: A
5-Consider this rxn which has G0 =+.4 KJ/mol

A+B--- C+D

1M A , 1M B , 0.1M C, 0.1M D are added to a container at room temperature . which direction will the
reaction move toward

A- its positive

B- o (zero)

C- negative

ANSWER: C

6- consider this reaction rxn which has G0= +.4KJ/mol

Succinate +FAD---- furmate +FADH2

21 mM of each compon are mixed & the reaction is allowed to come to equilibrium . which statement is
correct about the resulting concentration of FAD at equilibrium ?

A- [FAD]>[FADH2]

B- [FAD]<[FADH2]

C- [FAD]=[FADH2]

D- cant be determined from such information

ANSWER: A

7-consider this rxn which has G0=+0.4KJ/mol A+B--C+D 1M A, 1M B , 0.1M C, 0.1 D are added to a
container at a room temperature . which of the following statements is true?

A- DG<0 (NEGATIVE)

B-DG=O

C-DG >0(POSITIVE)

D-cant be determined from such information

ANSWER:A

8-For pure solids, the standard state is the pure solid itself

A- true
B- false

C- no way to know

ANSWER: A

9 – for pure liquids , the standard state is 1M concentration

A- true 

B- false 

C- maybe false or true depend on the type of liquid

D- its determined by doing experiment

ANAWER: B

10- for gases the standard state is :

A- 1M concentration

B- 1 atm

C- the gas it self

D- its determined by doing experiment

ANSWER: B

11- for solutes the standard state is

A- its determined by doing experiment

B- no way to know such information

C- depend on the type of solvent and solute

D- 1M concentration

ANSWER: D

12- I am performing a reaction ,A  B , with G0=+0.3 KJ/mol , I start the reaction with 10mM A and no B
, after allowing the reaction to proceed for 24 hrs at room temperature and atmospheric pressure , I
analyze a sample of the reaction mix to find I now have 1 mM A and 9mM B which of the following
conclusions should I make?

A-the reaction has reached equilibrium

B- I should come back later , the equilibrium hasn’t yet been reached

C- THE FORMATION OF B FROM A IS THERMODYNAMICALLY UNFAVORABLE , SO I SHOULD FIND

ANOTHER STARTING MATERIAL TO MAKE B
D – I must’ve screwed up, there is no way I could get that result with that G0

ANSWER : D

13-- I am performing a reaction ,A  B , with G0= - 5000KJ/mol , I start the reaction with 10mM A and
no B , after allowing the reaction to proceed for 24 hrs at room temperature and atmospheric pressure ,
I analyze a sample of the reaction mix to find I now have 8 mM A and 2mM B which of the following
conclusions should I make?

A-the reaction has reached equilibrium

B- I should come back later , the equilibrium hasn’t yet been reached

C- THE FORMATION OF B FROM A IS THERMODYNAMICALLY UNFAVORABLE , SO I SHOULD FIND

ANOTHER STARTING MATERIAL TO MAKE B

D – I must’ve screwed up, there is no way I could get that result with that G0

ANSWER: B

14- Biochemists use modified value for standard DG values because

ANSWER : the reagents aren’t at 1 molar concentration and ph=7

(the previous question from the information of the book)

15- ATP is more stable than ADP

A-true

B-false

ANSWER : B ,because ADP contain less group of similar electrostatic charge located in close proximity
to each other , less repulsion
16- in redox reaction the donator of electron is……….. but called……..

A-reduced, oxidizing agent

B-oxidized, reducing agent

C- reduced , reducing agent

D-oxidized, oxidizing agent

ANSWER:B

17-which best describes the DG for hydrolysis of creatine phosphate under cellular conditions in which
the concentration of creatine phosphate , creatine and phosphate . all equal 1M at 25C* . the G0=-43
KJ/mol.
A-DG< -43KJ/mol

B-DG=-43KJ/mol
C- -43KJ/mol<DG<0

D- DG>0

ANSWER : A

18-By definition , a spontaneous reaction is one in which

A- energy is released

B-energy is absorbed

C-the energy change is zero

D- the reaction happens quickly

E- energy is released and the reaction happens quickly

ANSWER :A

19-the standard state usually used in biochemistry (G0*) includes

A-all concentrations at 1M

B- all concentrations at 1M, except for [H+] , which is10^-7M

C- same as A but at 25C*

D- all of them are correct

ANSWER :B (from the book)

20-“metabolism “ refer to

A- the breakdown of larger molecules into smaller one

B-the production of larger molecules from smaller one

C-both

D-not mentioned

ANSWER :C

21- the production of larger molecules from smaller ones is called :

A-anabolism

B-catabolism

C –metabolism

D-not mentioned

ANSWER : A
22-Catabolism ………energy

A- release

B- absorb

C- both

D-not mentioned

ANSWER : A

23-catabolism is a(n)……….(oxidative /reductive )process that ……..(requires /release) energy .

ANSWER: oxidative , release

24- which of the following statements apply to anabolism?

A- proceeds in stages

B-requires energy

C-requires reducing agents

D-all of these

ANSWER :D

25- an oxidizing agent ……..( gain/ lose) electrons

ANSWER: Gain

26-during reduction reaction electrons (………)

ANSWER: are lost

27-in general , the anabolic pathway tend to involve oxidation reactions

A-true

B-false

ANSWER: B (in “general” the anabolic pathway include reduction reaction )

28-molecular oxygen is always a substrate in oxidation reactions

A-true

B-false
ANSWER : false ( example : NAD+ , FAD, NADP+ ..etc can replace the O2 in the oxidation reactions)

29-oxidation reaction involve the movement of electrons from one molecules to another .

A-True

B-false

ANSWER: A

30-when molecules are oxidized they lost electrons

A-true

B-false

ANSWER : A

31-reduction involve the gain of electrons

A- true

B-false

ANSWER: A

NOTE: Coenzyme A isn’t a carrier for electrons in redox reactions .

32- in biological redox reactions , H+ are usually transferred along with e-

A-true

B-false

ANSWER : A

33- which of the following coenzymes is derived from riboflavin ?

A- NAD+

B- FAD

C-coenzyme A

D-All of the above

E – non of the above

ANSWER: B
34-Which of the following is not a part of FAD?
A. Ribitol
B. isoalloxazine
C. adenine
D. ribose
E. nicotinamide

ANSWER: E

35-What happens to the entropy when ATP is hydrolysed to ADP?

A-increase
B-decrease
C-doesn’t change
D-no way to know with such information
ANSWER :A (hydrolyzed of ATP increase the randomness of molecules )
36-T/F: The oxidation of nutrients supplies the energy to produce ATP:

A-true

B-false

ANSWER :A

37-In order to drive the synthesis of ATP, the hydrolysis of an organic phosphate must have a _______
(equal/higher/lower) free energy change.

ANSWER : higher

38-Consider these reactions:

ATP --ADP + Pi DG°' = -31 kJ/mol

glucose-6-phosphate -- glucose + Pi DG°' = -13 kJ/mol

glucose + ATP -- glucose-6-phosphate + ADP DG°' = ?

A- -44
B- 18
C- -18
 D- 44

ANSWER: C

39-The body allows energy consuming reactions to occur by coupling them with reactions which
have a negative DG
A-true
B-false
ANSWER:A
40-ATP is a good source of energy to run metabolic reactions for all the following reasons,
except:

A. The pyrophosphate bond has a high energy of hydrolysis.

B. The sugar group is very reactive.
C. The bonds between the phosphates are acid anhydrides.
D. The phosphate groups can combine readily with other molecules.
E. All of these explain why ATP is a good energy source.
ANSWER: B
41-T/F: The number of degrees of freedom a molecule has is related to the numbers of resonant
structures it has.

A-true

B-false

ANSWER: A (the resonant structures include the randomness of molecules )

42-What happens to the entropy of a molecule as the number of resonance structures increases?

A-increase

B-decrease

C-doesn’t change

D-no relation ship between

ANSWER:A

43-Many cells oxidize fatty acids to produce ATP. If no ATP were produced, the DG°' of this process
would be
A. unchanged
B. a larger positive number
C. a larger negative number
D. impossible to determine

ANSWER: B

44-When we say that the efficiency of glycolysis is about 33% we mean that

A. 2 ATP are produced and 6 ATP are involved in the overall process
B. 2 ATP are produced in the oxidation of glucose, which contains six carbon atoms
C. the energy used to phosphorylate 2 ATP is 33% of the energy released in the process
D. all of these

ANSWER: C

45-T/F: The energy released during metabolism of nutrients can be used to synthesize ATP from ADP
and phosphate.

A-true

B-false

ANSWER :A

46-T/F: In order to initiate many metabolic pathways it is necessary to activate the starting materials.

A-True

B-false

Answer:A ( like in PPP the first reactant is G6P and the reaction of it is the rate limiting step , in the
reaction can’t go forward with activating it )

47-If the reaction A  B has DG = +25 Joule/mol and the reaction B ® C has DG = -15 Joule/mol, the
overall
energy change A  C will be:

A. -40 Joule/mol.
B. -15 Joule/mol.
C. +10 Joule/mol.
D. +40 Joule/mol.
E. You cannot determine the overall reaction from the given data.

ANSWER: C

48-Which of the following are examples of endergonic processes?

A. protein synthesis and active transport
B. protein synthesis and oxidation of carbohydrates
C. active transport and oxidation of carbohydrates
D. oxidation of fats and of carbohydrates

ANSWER: A

49-Which of the following are examples of exergonic processes?

A. protein synthesis and active transport
B. protein synthesis and oxidation of carbohydrates
C. active transport and oxidation of carbohydrates
D. oxidation of fats and of carbohydrates

ANSWER:D

50- The phosphorylation of ADP to produce ATP is endergonic because

A) a negatively charged ion is bonded to a molecule that already carries a negative charge

B) ATP is more stable than ADP

C) the entropy of the products is less than that of the reactants

D) polyphosphate chains are the storage form of phosphorus in living organisms

ANSWER:A

51-T/F: Spontaneous reaction always occurs at a relatively fast rate

A-True

B-false

ANSWER: B

52- The linking of an exergonic reaction to drive an endergonic reaction is called:

a. coupling

b. a state function

c. resonance

d. catabolism
ANSWER: A

53-Which of the following is not a mechanism used to activate substrates for further
metabolism?
A. Addition of a phosphate group.
B. Combination with a vitamin, such as coenzyme A.
C. Hydrolyzing a polymer into its component monomers.
D. Addition of a phosphate group or combining with a vitamin.
E. All of these processes activate substrates.
ANSWER: C

54-An example of an activation step in metabolism is

A. the hydrolysis of a triacylglycerol.
B. the cis-trans isomerization of retinal.
C. the formation of an acyl derivative of coenzyme A.
D. the formation of the peptide bond.
ANSWER: C
55-Metabolism takes place in stages
A. because enzymes cannot catalyze the process efficiently
B. and allows for efficient production and use of energy
C. because large free energy changes cannot occur in living organisms
D. to use highly unreactive compounds
ANSWER: C
56-

Which is the oxidizing agent?

A) Ethanol

B) NAD+

C) Acetaldehyde

D) NADH

E) H+

ANSWER:B
57-

Which molecule is oxidized??

A) Ethanol

B) NAD+

C) Acetaldehyde

D) NADH

E) H+

ANSWER:B

58-

Which molecule loses electrons?

A) Ethanol

B)NAD+

C)Acetyldehyde

D)NADH

E)H+
ANSWER:A

59- During oxidation of ethanol

A) electrons are lost

B) electrons are gained

C) electrons may either be lost or gained

D) hydrogen is formed

ANSWER:A

60- Which of the following is false?

A) molecular oxygen is always a substrate in oxidation reactions

B)Oxidation reactions involve the movement of electrons from one molecule to another

C)When a molecule is oxidized, it loses electrons

D)Reduction involves the gain of electrons

ANSWER:A

61- The conversion of NAD+ to NADH is an example of reduction because

A) the pyridine ring loses electrons (and a hydrogen)

B) the pyridine ring gains electrons (and a hydrogen)

C) the adenine ring loses electrons

D) the adenine ring gains electrons

ANSWER:A
‫ فقط احفظ اسم الحلقة‬, ‫ال جديد‬

1. D-Glucose and D-Talose

A. Diasteriomers B. Anomers C. Epimers D. Enantiomers E. Not isomers

2. D-Allose and D-Altrose

A. Diasteriomers B. Anomers C. Epimers D. Enantiomers E. Not isomers

3. D-Ribose and D-Talose

A. Diasteriomers B. Anomers C. Epimers D. Enantiomers E. Not isomers
4. α-D-glucopyranose and β-D-glucopyranose
A. Diasteriomers B. Anomers C. Epimers D. Enantiomers E. Not isomers

1. Fructose is called a(n):

a. Ketohexose
b. Aldohexose
c. Aldopentose
d. Ketopentose
2. Glyceraldehyde is a carbphydrate that contains___carbon atoms.
a. 3
b. 4
c. 5
d. 6
3. In addtion to containing alcohol functional groups, a monossacharide that is classified as an
aldose would also contain what specific functional group?
a. Carbonyl
b. Aldehyde
c. Carboxylic acid
d. Ketone
4. Glucose and Galactose are ___ of each other?
a. cis-trans isomers
b. epimers
c. anomers
d. enantiomer
The correct Relationship between D-glucose And D-allose is:
A. anomers B. epimers    C. cis/trans isomers    D. enantiomers   E. all of the above
DONE BY : A-Neem Al-
Sawaie

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finita est”
-Ludwig van Beethoven