Scribd Logo
Upload

Welcome to Scribd!

  • 1.2 Enzymatic Degradation of Cellulose: Dci DX

    Uploaded by

    pricett
    23 views4 pages
    1. Enzymatic degradation of cellulose involves breaking down polymeric chains of glucose units (cellulose) into smaller polymers and eventually glucose. 2. Endoglucanase and Cellobiohydrolase enzymes break bonds within cellulose chains, producing smaller polymer fragments. Beta-glucosidase then breaks these fragments down into glucose. 3. Mathematical equations are provided to model the rates of formation and degradation of polymer fragments of different lengths (C1-C4) by the various enzymes. Probabilities of bonds being cut are also considered in the equations.

    Original Description:

    Original Title

    Enzymes Q1.2

    Copyright

    © Attribution Non-Commercial (BY-NC)

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    1. Enzymatic degradation of cellulose involves breaking down polymeric chains of glucose units (cellulose) into smaller polymers and eventually glucose. 2. Endoglucanase and Cellobiohydrolase enzymes break bonds within cellulose chains, producing smaller polymer fragments. Beta-glucosidase then breaks these fragments down into glucose. 3. Mathematical equations are provided to model the rates of formation and degradation of polymer fragments of different lengths (C1-C4) by the various enzymes. Probabilities of bonds being cut are also considered in the equations.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    23 views4 pages

    1.2 Enzymatic Degradation of Cellulose: Dci DX

    Uploaded by

    pricett
    1. Enzymatic degradation of cellulose involves breaking down polymeric chains of glucose units (cellulose) into smaller polymers and eventually glucose. 2. Endoglucanase and Cellobiohydrolase enzymes break bonds within cellulose chains, producing smaller polymer fragments. Beta-glucosidase then breaks these fragments down into glucose. 3. Mathematical equations are provided to model the rates of formation and degradation of polymer fragments of different lengths (C1-C4) by the various enzymes. Probabilities of bonds being cut are also considered in the equations.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 4

    1.

    2 Enzymatic degradation of cellulose

    Summary of reactions

    Where –

    Pi - is a polymer unit of length I where I is a real number greater than 2

    P2 – is cellobiose (glucose dimer )

    P1 - is glucose

    Note : In the ensuing discussion the concentration rate of a particular species is


    simply the rate v multiplied by the concentration

    dci
    a) The rate of formation of polymers of a particular length i units ( dx ) will be
    due to the rate at which the enzymes Endoglucanase (EG) and
    Cellobiohydrolase (CBH) cuts the polymeric chain times the concentration
    of the polymeric chain that is cut minus what is already there.

    It is assumed that EG can cut chains of original length 3or greater and that each
    bond in the chain has equal probability of being cut.
    Figure 1 Probability of a c4 chain being cut to form c3 chain

    Figure 2 probability of a c4 chain being cut

    dc 3
    Hence looking ( dt ) enzyme (EG) will break a a chain lengh of length 4 to form of
    a polymer of chain length 3 and 1 ,while polymers of chain length 4 disappears
    dueto it being attacked .Chain length 4 can be attacked at any linkage (hence the
    probability of 3) .

    A Cellobiohydrolase (CBH) enzyme cuts off a single unit of length 2, from one end
    (the reducing end) of the polymeric chain to produce a ( c3 )

    Figure 3 Enzyme (BH) at work

    While any ( c3 ) present will be attached and hence the concentration falls.

    b) For
    dc 2
    The rate of formation of polymers with 2 units ( dx ) will be due to the sum
    of the rates at which the enzymes Endoglucanase (EG) and Cellobiohydrolase
    (CBH) cuts the polymeric chain times the concentration of the polymeric chains
    present minus the rate at which Beta-glucosidases (BG) removes the ( c2 )
    polymer units that are formed to form glucose.

    Looking closer at the formulas -

    The rate of formation of ( c2 ) is


    doubled because the enzyme (EG) can cut the chain at either end . This is the
    “feed” material for the enzymes (EG) and (BH) to make smaller chains (in
    particular ( c2 ) .Note (BH) removes ( c2 ) )

    In this case when there are


    chain lengths of 4 ( c4 ) the rate is doubled because the chain is “symmetrical “
    and can be cut at either end.

    This term is the rate at which Beta-


    glucosidases (BG) removes the ( c2 ) polymer units that are formed to form
    glucose. That is why it is subtracted.
    c) Inspecting each term

    The rate of formation of ( c1 ) is


    doubled because with the enzyme (EG) can cut the chain at either end .This is the
    “feed” material for the enzymes EG and BH to make smaller chains (in particular
    ( c1 ) )

    For chain lengths of 3 ( c3 ) the


    enzyme (BH) will attack a ( c3 ) polymer to form a ( c2 ) and a ( c1 ) hence the
    multiplication of the rate with ( c3 )

    the last term is doubled due to the fact


    that the molecule is ‘’symmetrical ‘’ and there is equal chance of splitting a ( c 2 )
    to form a ( c1 )

    You might also like