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Biochromatography Portfolio Guide

Media and Prepacked Columns

Merck Millipore is a business of


Media Type Product Description Ligand Application Product Characteristics Prepacked Columns Catalog No. Bulk Media Catalog No.
Eshmuno® Q media Acidic, neutral, recombinant • Mean particle size: d50 85 μm MiniChrom Columns: 10 ml 1200790010
Base matrix: Hydrophilic proteins and peptides from •D  ynamic binding capacity: 1 ml (8 *20 mm) 1250650001 100 ml 1200790100
Polyvinyl Ether different sources, (including 80 mg BSA/ml settled resin at 2 min residence time 5 ml (8 *100 mm) 1250740001 500 ml 1200790500
plasma proteins). DNA removal. • pH stability range (working): 2-12 5L 1200795000
RoboColumns®:
Designed specifically for • p H stability range (CIP): 0-14 0.2 ml (5 *10 mm) 1251330001
highly productive downstream • P ressure limit 8 bar 0.6 ml (5 *30 mm) 1251410001
bioprocessing. • L inear flow rate up to 1000 cm/h < 2.5 bar net pressure Chromabolt® columns prepacked
with Eshmuno® Q media:
Separation at high flow rates.
10 cm (inner diameter) CHRPC10791
instead of high flowrate
20 cm (inner diameter) CHRPC10792
applications.
32 cm (inner diameter) CHRPC10793

Fractogel® EMD TMAE Acidic, neutral, recombinant • Particle size: 40-90 µm MiniChrom Columns: 10 ml 1168810010
(M) media proteins and peptides from • Protein binding capacity about 100 mg BSA/ml of gel 1 ml (8*20 mm) 1250540001 100 ml 1168810100
Base matrix: Methacrylate different sources, (including 5 ml (8*100 mm) 1250690001 500 ml 1168810500
• pH stability range pH 2-12, Pressure limit 8 bar
plasma proteins). DNA and virus 5L 1168815000
• Linear flow rate up to ~250 cm/h RoboColumns®:
removal, plasmid isolation.
0.2 ml (5*10 mm) 1250840001
0.6 ml (5*30 mm) 1251360001
Strong anion exchangers (AEX)

Fractogel® EMD TMAE • Particle size: 20-40 µm (S) type MiniChrom Columns: 10 ml 1168870100
Trimethylammoniumethyl

(S) media • Protein binding capacity about 100 mg BSA/ml of gel 1 ml (8*20 mm) 1250570001 100 ml 1168870500
Base matrix: Methacrylate 5 ml (8*100 mm) 1250710001 500 ml 1168875000
• pH stability range pH 2-12, Pressure limit 8 bar
5L
• Linear flow rate up to ~150 cm/h (S-type) RoboColumns®:
TMAE

0.2 ml (5*10 mm) 1250860001


0.6 ml (5*30 mm) 1251360001

Fractogel® EMD TMAE • Particle size: 40-90 µm MiniChrom Columns: 10 ml 1103160010


Hicap (M) media • Protein binding capacity about 180 mg BSA/ml of gel 1 ml (8*20 mm) 1250550001 100 ml 1103160100
Base matrix: Methacrylate 5 ml (8*100 mm) 1250700001 500 ml 1103160500
• pH stability range pH 2-12, Pressure limit 8 bar
5L 1103165000
• Linear flow rate up to ~250 cm/h RoboColumns®:
0.2 ml (5*10 mm) 1250850001
0.6 ml (5*30 mm) 1251370001

Chromabolt® columns prepacked


with Fractogel® EMD TMAE Hicap
media:
10 cm (inner diameter) CHRPC18811
20 cm (inner diameter) CHRPC18812
32 cm (inner diameter) CHRPC18813

Fractogel® EMD TMAE • Particle size: 40-90 µm MiniChrom Columns: 10 ml 1168850010


Medcap (M) media • Protein binding capacity about 150 mg BSA/ml of gel 1 ml (8*20 mm) 1250780001 100 ml 1168850100
Base matrix: Methacrylate 5 ml (8*100 mm) 125056.0001 500 ml 1168850500
• pH stability range pH 2-12, Pressure limit 8 bar
5L 1168855000
• Linear flow rate up to ~180 cm/h RoboColumns®:
0.2 ml (5*10 mm) 1251440001
0.6 ml (5*30 mm) 1251500001

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Media Type Product Description Ligand Application Product Characteristics Prepacked Columns Catalog No. Bulk Media Catalog No.
Fractogel® EMD DEAE Acidic, neutral, recombinant • Particle size: 40-90 μm MiniChrom Columns: 10 ml 1168830010
(M) media proteins and peptides from • Protein binding capacity about 100 mg BSA/ml of gel 1 ml (8*20 mm) 1250580001 100 ml 1168830100
Base matrix: Methacrylate different sources, (including 5 ml (8*100 mm) 1250790001 500 ml 1168835000
• pH stability range pH 2-13
plasma proteins) and DNA 5L
• Pressure limit 8 bar RoboColumns®:

Diethylaminoethyl
removal.
• Linear flow rate up to 170 cm/h 0.2 ml (5*10 mm) 1251450001
Due to the titration behaviour, 0.6 ml (5*30 mm) 1251510001

DEAE
Weak anion exchangers (AEX)

the ion exchange media can be


used from pH 2 up to pH 9.5.
Fractogel® EMD DEAE Blood fractionation. • Particle size: 20-40 μm 10 ml 1168880010
(S) media • Protein binding capacity about 100 mg BSA/ml of gel 100 ml 1168880100
Base matrix: Methacrylate 500 ml 1168880500
• pH stability range pH 2-13
5L 1168885000
• Pressure limit 8 bar
• Linear flow rate up to 80 cm/h

Fractogel® EMD DMAE Strong acidic and neutral • Particle size: 40-90 μm MiniChrom Columns: 10 ml 1168840010
(M) media
Dimethylaminoethyl
proteins (e.g. plasma proteins), • Protein binding capacity about 100 mg BSA/ml of gel 1 ml (8*20 mm) 1250590001 100 ml 1168840100
Base matrix: Methacrylate peptides, and nucleic acids. 5 ml (8*100 mm) 1250800001 500 ml 1168840500
• pH stability range pH 2-13
5L 1168845000
Due to the titration behavior, RoboColumns®:
DMAE
• Pressure limit 8 bar
the ion exchange media can be • Linear flow rate up to 170 cm/h 0.2 ml (5*10 mm) 1251460001
used from pH 2 up to pH 8.5. 0.6 ml (5*30 mm) 1251520001
Blood fractionation.

Eshmuno® S media Strong basic and neutral • Particle size d50: 85 µm MiniChrom Columns: 10 ml 1200780010
Base matrix: Hydrophilic proteins (e.g. antibodies) and • P rotein binding capacity 135-195 mg lysozyme/ml of 1 ml (8*20 mm) 1250640001 100 ml 1200780100
Polyvinyl Ether peptides, designed specifically settled resin, IgG Dynamic Capacity ≥ 60 mg/ml 5 ml (8*100 mm) 1250730001 500 ml 1200780500
for highly productive (2 min residence time, 10% BT) 5L 1200785000
RoboColumns®:
downstream bioprocessing.
• pH stability range (working): 2-12 0.2 ml (5*10 mm) 1251320001
Superior binding capacity in • pH stability range (CIP): 0-14 0.6 ml (5*30 mm) 1251400001
Strong cation exchangers (CEX)

direct capture applications, at • Pressure limit 8 bar


moderate ionic strength. Chromabolt® columns prepacked
• Linear flow rate up to 1000 cm/h < 2.5 bar net pressure
High flowrate applications. with Eshmuno® S media:
10 cm (inner diameter) CHRPC10781
Sulfoisobutyl

20 cm (inner diameter) CHRPC10782


32 cm (inner diameter) CHRPC10783
SO3

Eshmuno® CPX media Intermediate or polishing • Mean particle size: 50 µm MiniChrom Columns: 10 ml 1200830010
Base matrix: Hydrophilic purification of monoclonal • P rotein binding capacity 110 mg plgG/ml settled resin 1 ml (8*20 mm) 1251560001 100 ml 1200830100
Polyvinyl Ether antibodies. (4 min residence time, 5% BT) 5 ml (8*100 mm) 1251570001 500 ml 1200830500
5L 1200835000
High aggregate removal • pH stability (working): 2-12
RoboColumns®:
efficiency. • pH range (CIP): 0-14 0.2 ml (5*10 mm) 1251580001
Excellent IgG dynamic binding • Pressure limit 8 bar 0.6 ml (5*30 mm) 1251590001
capacity. • L inear flow rate up to 500cm/h
(<3.0 bar net pressure)
Wide pH and conductivity
operating window.

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Media Type Product Description Ligand Application Product Characteristics Prepacked Columns Catalog No. Bulk Media Catalog No.
Fractogel® EMD SO3- Strong basic and neutral • Particle size: 40-90 μm MiniChrom Columns: 10 ml 1168820010
(M) media proteins (e.g. antibodies) and • Protein binding capacity about 130 mg lysozyme/ml of gel 1 ml (8*20 mm) 1250610001 100 ml 1168820100
Base matrix: Methacrylate peptides. 5 ml (8*100 mm) 1250720001 500 ml 1168820500
• pH stability range pH 1-13
5L 1168825000
• Pressure limit 8 bar RoboColumns®:
• Linear flow rate up to ~200 cm/h 0.2 ml (5*10 mm) 1250900001
0.6 ml (5*30 mm) 1251390001

Chromabolt® colums prepacked

Sulfoisobutyl
with Fractogel® EMD SO3 (M)
media: CHRPC18821

SO3
10 cm (inner diameter) CHRPC18822
Strong cation exchangers (CEX)

20 cm (inner diameter) CHRPC18823

Fractogel® EMD SO3- Strong basic and neutral • Particle size: 20-40 µm 10 ml 1168900010
(S) media proteins (e.g. antibodies) and • Protein binding capacity about 150 mg lysozyme/ml of gel 100 ml 1168900100
Base matrix: Methacrylate peptides. 500 ml 1168900500
• pH stability range pH 1-13
5L 1168905000
• Pressure limit 8 bar
• Linear flow rate up to ~80 cm/h

Fractogel® EMD Strong basic and neutral • Particle size: 40-90 μm MiniChrom Columns: 10 ml 1148940010
SE Hicap (M) media proteins (e.g. antibodies) and • Protein binding capacity about 160 mg lysozyme/ml of gel 1 ml (8*20 mm) 1250600001 100 ml 1148940100
Base matrix: Methacrylate peptides. 5 ml (8*100 mm) 1250810001 500 ml 1148940500
• pH stability range pH 1-13
5L 1148945000
• Pressure limit 8 bar RoboColumns®: 1251470001
0.2 ml (5*10 mm)
Sulfoethyl

• Linear flow rate up to ~220 cm/h 1251530001


0.6 ml (5*30 mm)
SO3

Chromabolt® columns prepacked


with Fractogel® EMD SE Hicap
(M) media:
10 cm (inner diameter) CHRPC18941
20 cm (inner diameter) CHRPC18942
32 cm (inner diameter) CHRPC18943

Fractogel® EMD COO- Basic and neutral proteins and • Particle size: 40-90 μm MiniChrom Columns: 10 ml 1168860010
(M) media peptides. • Protein binding capacity about 100 mg lysozyme/ml of gel 1 ml (8*20 mm) 1250620001 100 ml 1168860100
Base matrix: Methacrylate 5 ml (8*100 mm) 1250820001 500 ml 1168860500
Weak cation exchangers

Due to the titration behaviour, • pH stability range pH 1-12


5L 1168865000
the ion exchange media can be • Pressure limit 8 bar RoboColumns®:
Carboxyethyl

used from pH 6 up to pH 12. • Linear flow rate up to~300 cm/h 0.2 ml (5*10 mm) 1251480001
0.6 ml (5*30 mm) 1251540001
COO-
(CEX)

Efficient for difficult aggregate


removal. Chromabolt® columns prepacked
with Fractogel® EMD COO (M)
media:
10 cm (inner diameter) CHRPC18861
20 cm (inner diameter) CHRPC18862
32 cm (inner diameter) CHRPC18863

Eshmuno® HCX media Direct capture and intermediate • Mean particle size: d50 , 85 µm MiniChrom Columns: 10 ml 1200870010
sulfo + carboxy + phenyl
salt-tolerant (multi-mode

Base matrix: Hydrophilic polishing from clarified feed • P rotein binding capacity (5 min residence time, 10 % 1 ml (8*20 mm) 1250660001 100 ml 1200870100
Polyvinyl Ether stocks. breakthrough) ≥ 50 mg pIgG /ml settled resin 5 ml (8*100 mm) 1250750001 500 ml 1200870500
5L 1200875000
High dynamic binding • pH stability range (working): 2-12 RoboColumns®:
CEX High

media)

capacity at high conductivity • pH stability range (CIP): 2-14 0.2 ml (5*10 mm) 1251340001
applications. • Pressure limit 8 bar 0.6 ml (5*30 mm) 1251420001
• Linear flow rate up to 1000 cm/h (<2.5 bar net pressure)

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Media Type Product Description Ligand Application Product Characteristics Prepacked Columns Catalog No. Bulk Media Catalog No.
Hydrophobic interaction Fractogel® EMD Phenyl Weak hydrophobic • Particle size: 20-40 µm 10 ml 1161970010
chromatography (HIC) (S) media chromatography support for • Protein binding capacity about 20 mg ovalbumin/ml of gel 100 ml 1161970010
Base matrix: Methacrylate purification of proteins and 500 ml 1161970500
• pH stability range pH 2-12
antibodies, membrane proteins.
• Pressure limit 8 bar

Phenyl
• Linear flow rate up to 80 cm/h

Fractogel® EMD Chelate Various proteins, e.g. histidine- • Particle size: 40-90 µm MiniChrom Columns: 10 ml 1103380010
Immobilized metal affinity

(M) media
chromatography (IMAC)

tailed recombinant proteins or • Protein binding capacity about 60 mg lysozyme/ml of gel 1 ml (8*20 mm) 1250630001 100 ml 1103380100

Iminodiacetic acid
Base matrix: Methacrylate proteins with exposed histidine 5 ml (8*100 mm) 1250830001 250 ml 1103380250
residues. •M etal ion binding capacity about 75 μmol copper/ml 500 ml 1103380500
of gel RoboColumns®: 5L 1103385000
The metal ion can coordinate to
• pH stability range pH 1-12 0.2 ml (5*10 mm) 1251490001
Non-bonding electron pairs of
• Pressure limit 8 bar 0.6 ml (5*30 mm) 1251550001
the amino acid side chains.
• Linear flow rate up to ~240 cm/h

Eshmuno® A media High capacity Protein A • Particle size: ~50 µm MiniChrom Columns: 10 ml 1200890010
Merck Millipore proprietary

Base matrix: affinity chromatography • P rotein Binding capacity 40-55mg/ml at 3-6 min RT and 1 ml (8*20 mm) 1251600001 100 ml 1200890100
ligand recombinantely

Hydrophilic Polyvinyl Ether


expressed in E. coli

resin for the purification of 5% breakthrough for mAbs 5 ml (8*100 mm) 1251610001 500 ml 1200890500
Fc-containing proteins, 5L 1200895000
• pH stability range 2.5-8.0 RoboColumns®:
including but not limited to 1251620001 10 L 1200899010
• Pressure limit 8 bar 0.2 ml (5*10 mm)
monoclonal antibodies 1251630001
• Linear flow rate >500 cm/hr 0.6 ml (5*30 mm)

ProSep® Ultra Plus Affinity purification of mAbs, • Particle size: 60 µm MiniChrom Columns: 2 ml 175118822
media rSPA = recombinant designed for cost effective, 1 ml (8*20 mm) 1250670001 10 ml 175118824
Protein A affinity chromatography

• Dynamic Binding Capacity: >50 mg/ml


Base matrix: Controlled Pore structurally large scale purification of high 5 ml (8*100 mm) 1250760001 100 ml 175118827
• pH stability range pH 1-8.5
Glass (CPG) conserved natural titre mAbs. 1L 175118830
• Pressure limit 10 bar RoboColumns®:
protein A (E. coli) 1251350001 5L 175118833
• L inear Flow Rate up to 500 cm/h at 2 bar at 20 cm 0.2 ml (5*10 mm)
(60um) CPG with 1251430001 10 L 175118835
bed height 0.6 ml (5*30 mm)
~800Å 25 L 175118834
sized pores that
allow high flow rates
at relatively low
back pressures

ProSep®-vA Ultra Affinity purification of mAbs. • Particle size: 75-125 µm 2 ml 115115822


media nPA = native animal • Dynamic Binding Capacity: ~35 mg/ml 10 ml 115115824
Base matrix: Controlled free protein A from • pH stability range pH 1-8.5 100 ml 115115827
Pore Glass Staphylococcus • Pressure limit 10 bar 500 ml 115115829
aureus (100 µm) • L inear Flow Rate up to 500 cm/h at 2 bar at 20 cm 1L 115115830
CPG with ~700Å bed height 5L 115115833
pores 10 L 115115835

ProSep®-vA High • Particle size: 100 µm 2 ml 113115822


Capacity media nPA = native animal •D  ynamic Binding Capacity: >20 mg/ml at 10% 10 ml 113115824
Base matrix: Controlled free protein A from breakthrough and 3-6 minute residence time 100 ml 113115827
Pore Glass Staphylococcus • pH stability range pH 1-8.5 500 ml 113115829
aureus (100 µm) • Pressure limit 10 bar 1L 113115830
CPG with ~1000Å • Linear Flow Rate up to 500 cm/h at 2 bar at 20 cm 5L 113115833
pores bed height 10 L 113115835
10 L 115115835

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Media Type Product Description Ligand Application Product Characteristics Prepacked Columns Catalog No. Bulk Media Catalog No.
Fractogel® EMD BioSEC Separation of proteins in the • Particle size: 20-40 µm 150 ml 1103170150
(S) media range between 5kDa and 250 ml 1103170250
chromatography
• pH stability range pH 1-12

Modified pore
Size exclusion

Base matrix: Methacrylate 1000kDa. 5L 1103175000


• Pressure limit 8 bar

structure
(SEC)

• Linear flow rate up to 100 cm/h

PharmPrep® P sorbent Small peptides (insulin), APIs, • Spherical high performance sorbent Ready to use columns for method 10, 100, 1000, 5000g*
Base matrix: Spherical antibiotics, intermediates. • Mean pore size: 10 nm development
PharmPrep® P sorbent 100
particles of silica Scout columns 250x4.6 mm
• Pressure PharmPrep® P Si <25 bar (10 µm), 10 bar RP-18e, 10 μm 1.19995
• P ressure PharmPrep® 100 RP-18e <40 bar PharmPrep® P sorbent 100 RP-18e,
(10 µm) PharmPrep® P sorbent 100
10 μm 1205710001

RP-18e, 10 µm, 20 µm
1.19996

Si 100, 10 µm, 20 µm
RP-18e, 20 μm
PharmPrep® P sorbent 100 RP-18e,
20 μm 1205720001 PharmPrep® P sorbent
Si 100, 10 μm 1.19681
Ready to use columns for
preparative chromatography PharmPrep® P sorbent
HIBAR® prepacked columns Si 100, 20 μm 1.19682
250x25 mm
Normal phase/Reversed phase chromatography and Silica gel

PharmPrep® P sorbent 100 RP-18e,


10 μm 1205730001
PharmPrep® P sorbent 100 RP-18e,
20 μm 1205740001

LiChroprep® sorbent APIs, antibiotics, steroides, • Irregular particles Ready to use columns for preparative On demand 100, 500, 1000g*
Base matrix: Irregular intermediates. • 1 5-25 µm, 25-40 µm, 40-63 µm chromatography HIBAR® prepacked
particles of silica LiChroprep® sorbent
• Mean pore size: 10 nm columns CN 40-63 µm 1.13959
250x25 mm, 250x50 mm
LiChroprep® sorbent
RP-18e, 15-25 µm 1.13901

LiChroprep® sorbent
RP-18e, 25-40 µm 1.09303

LiChroprep® sorbent
RP-8, CN, NH2, Diol

RP-18e, 40-63 µm 1.13900


RP-18, Si 60

LiChroprep® sorbent
RP-8, 40-63 µm 1.09362

LiChroprep® sorbent
Diol, 40-63 µm 1.13973
LiChroprep® sorbent
NH2, 40-63 µm 1.13974

LiChroprep® sorbent
Si 60, 15-25 µm 1.09336

LiChroprep® sorbent
Si 60, 25-40 µm 1.09390

LiChroprep® sorbent
Si 60, 40-63 µm 1.13905

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Media Type Product Description Ligand Application Product Characteristics Prepacked Columns Catalog No. Bulk Media Catalog No.
Silica gel Natural ingredients, vitamins, • For normal phase chromatography, 500g to 400kg*
Base matrix: Irregular F&B. filtration, flash chromatography Silica gel Si 60, 15-40 µm 1.13905
Normal phase/Reversed phase chromatography and Silica gel
particles of silica • Particle size: Silica gel Si 60, 40-63 µm 1.09385
200 - 500 μm

Si 60
63 - 200 μm Silica gel Si 60, 63-100 µm 1.15101
40 - 63 μm Silica gel Si 60, 63-200 µm 1.07734
15 - 40 μm Silica gel Si 60, 200-500 µm 1.07733
• Mean pore size: 6 nm
• pH (10 % aq. Solution) 6.5 - 7.5
Aluminium Oxide Intermediates • For normal phase chromatography 1, 2, 5, 50kg*

60 basic, 90 acidic, neutral, basic, 150 basic


Base matrix: Irregular • High pH stability in the alkaline range.
particles of aluminia Aluminium Oxide 60, active, 1.01067
Anion and cation properties basic 63-200 µm
• Mean pore size: 6 nm/15 nm
• pH stability pH 8.5-10.5 Aluminium Oxide 150, basic 1.01061
63-200µm

Aluminium Oxide 90, active 1.01076


basic, 63-200 µm

Aluminium Oxide 90, active 1.01077


neutral, 63-200 µm

Aluminium Oxide 90, active 1.01078


acidic, 63-200 µm

Aluminium Oxide 90, 1.01097


standardized, 63-200 µm
Chromabolt® Prepacked, prevalidated See resin for resin characteristics. Chromabolt® colums prepacked
prepacked columns chromatography columns, All columns are packed in a ISO 8 Clean Room using with Eshmuno® S media:
with Merck Millipore resins automated packing and sanitizing equipment. This ensures 10 cm (inner diameter) CHRPC10781
for early clinical stage, process a high quality reproducible and robust manufacturing 20 cm (inner diameter) CHRPC10782
development and scale up. process. 32 cm (inner diameter) CHRPC10783
The columns are in sizes 10, 20 and 32 cm (ID) with fixed
Chromabolt® columns prepacked
20 cm bed heights.
with Fractogel® EMD TMAE Hicap
The columns have been validated to maintain specifications media:
after undergoing ISTA-1E testing to simulate shipping 10 cm (inner diameter) CHRPC18811
conditions. 20 cm (inner diameter) CHRPC18812
Chromatography columns prepacked

32 cm (inner diameter) CHRPC18813


Each column comes with the following:
with Merck Millipore resins

• User Guide Chromabolt® columns prepacked


• Certificate of Quality with Fractogel® EMD SO3 media:
10 cm (inner diameter) CHRPC18821
•C ertificate of Quality of resin lot packed in the column
20 cm (inner diameter) CHRPC18822
see resin

32 cm (inner diameter) CHRPC18823


The 20/32 cm Chromabolt® columns are shipped with a
50 mL sample of resin that is packed in the column
Chromabolt® columns prepacked
with Eshmuno® Q media:
10 cm (inner diameter) CHRPC10791
20 cm (inner diameter) CHRPC10792
32 cm (inner diameter) CHRPC10793

Chromabolt® columns prepacked


with Fractogel® EMD COO (M):
10 cm (inner diameter) CHRPC18861
20 cm (inner diameter) CHRPC18862
32 cm (inner diameter) CHRPC18863

Chromabolt® columns prepacked


with Fractogel® EMD SE Hicap (M):
10 cm (inner diameter) CHRPC18941
20 cm (inner diameter) CHRPC18942
32 cm (inner diameter) CHRPC18943

For further details, please see our product specific brochures and Data Sheets * cat. no. needs to be complemented by the 4-digit volume indication: 10g: 0010; 100g: 0100; 1000g: 1000; 5000g: 5000 7
Media Type Product Description Ligand Application Product Characteristics Prepacked Columns Catalog No. Bulk Media Catalog No.
Chromabolt® Prepacked, prevalidated See resin for resin characteristics. Chromabolt® columns prepacked
prepacked columns chromatography columns, All columns are packed in a ISO 8 Clean Room using with Fractogel® EMD TMAE (M): CHRPC18891
with Merck Millipore resins automated packing and sanitizing equipment. This ensures 10 cm (inner diameter) CHRPC18892
for early clinical stage, process a high quality reproducible and robust manufacturing 20 cm (inner diameter) CHRPC18893
Chromatography columns prepacked

development and scale up. process. 32 cm (inner diameter)


with Merck Millipore resins

The columns are in sizes 10, 20 and 32 cm (ID) with fixed


Chromabolt® columns prepacked
20 cm bed heights.
with Fractogel® EMD DEAE (M):
The columns have been validated to maintain specifications 10 cm (inner diameter) CHRPC18831
after undergoing ISTA-1E testing to simulate shipping 20 cm (inner diameter) CHRPC18832

see resin
conditions. 32 cm (inner diameter) CHRPC18833

Each column comes with the following:


• User Guide
• Certificate of Quality
•C ertificate of Quality of resin lot packed in the column

The 20/32 cm Chromabolt® columns are shipped with a


50 mL sample of resin that is packed in the column

To place an order or receive


technical assistance
In Europe, please call Customer Service:
France: 0825 045 645
Germany: 069 86798021
Italy: 848 845 645
Spain: 901 516 645 Option 1
Switzerland: 0848 645 645
United Kingdom: 0870 900 4645
For other countries across Europe,
please call: +44 (0) 115 943 0840

Or visit: www.merckmillipore.com/offices

For Technical Service visit:


www.merckmillipore.com/techservice

www.merckmillipore.com /biochromatography

Merck Millipore, the M logo, Chromabolt, Eshmuno, Fractogel, HIBAR, LiChroprep, PharmPrep and ProSep are registered trademarks of Merck KGaA, Darmstadt, Germany.
Robocolumn is a registered trademark of Atoll GmbH, Weingarten. Lit code: PF4422EN00 Version 2.0 DP SBU-11-05184 © 2015 EMD Millipore Corporation, Billerica, MA, USA. All rights reserved.

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