QUESTION BANK

BIOCHEMISTRY I (2230003)

CHAPTER 1
1. Discuss the transport mechanism across cell membrane.
2. Enumerate various cell organelles and discuss about mitochondria.
3. Describe structure of cell membrane with diagram.
4. Describe structure and functions of different cell organelles of eukaryotic cell.
5. Short note on: Facilitated diffusion.
CHAPTER 2 & 3
1. Classify carbohydrates. Describe the structure and function of polysaccharides.
2. Explain following terms with significance:
(i) Epimers
(ii) Invert sugar
(iii) Muta-rotation
3. How galactose is metabolized in the body?
4. Describe HMP shunt or hexose monophosphate or pentose phosphate pathway.
Give its significance.
5. Explain TCA cycle or Krebs cycle or tricarboxylic acid cycle along with
energetics and amphibolic nature of it.
6. Discuss the role of hormones in maintenance of blood sugar/glucose level.
7. Differentiate: Gluconeogenesis and glycolysis.
8. What are carbohydrates and give classification of carbohydrates with examples.
Describe in brief chemical reactions of monosaccharides
9. Define the following terminology:
(a) Glycolysis
(b) Gluconeogenesis
(c) Anomers
(d) Prosthetic group
10. Discuss aerobic glycolysis with examples.
11. Describe the structure and functions of mucopolysaccharides.
12. Discuss glycolysis with its energetics.
13. Discuss importance of Cori’s cycle.
14. Discuss the synthesis of glucose from non-carbohydrate sources.
15. Write a note on glycogen storage disease.
 16. Define and classify carbohydrates and chemical reactions of monosaccharides.
17. Explain glycogenolysis with metabolic reactions.
18. Explain glycogenesis in detail.
19. Explain the process of gluconeogenesis and special pathway involved in it.
20. Write a note on genetic disorders of carbohydrate metabolism.
21. Define mutarotation and epimers. Which test will you perform to detect the
presence of reducing sugar. Explain the principle of the same test.
CHAPTER 2 & 4
1. What are essential fatty acids and write functions and deficiency symptoms.
2. Explain carnitine shuttle pathway of fatty acids. What is metabolic water?
3. Classify lipids with example. Add note on triglycerides.
4. Describe various chemical tests to check the purity of fats and oils.
5. Classify and explain phospholipids.
6. Discuss in detail cholesterol biosynthesis.
7. Write about biosynthesis and utilization of ketone bodies in diabetes.
8. How bile acids are formed in the body and write their significance.
9. Differentiate: α-oxidation and β-oxidation.
10. Differentiate: saturated and unsaturated fatty acids.
11. Explain reactions and utilization of ketone bodies.
12. Discuss β-oxidation of fatty acids with energetics.
13. What are bioenergetics and discuss their biochemical importance.
14. Define:
(i) Iodine number
(ii) Saponification number
(iii) Acid number
(iv) Rancidity
15. Short note on:
(a) Lipoproteins
(b) Essential fatty acids
16. Describe the structure of steroids. Write a note on functions of cholesterol.
17. Write a note on lipid storage disease.
18. Write an account of classification of lipids with suitable example.
19. Discuss ketogenesis with reactions. Explain significance of ketogenesis. Add note
on demerits caused due to overproduction of ketone bodies.
20. Write a note on cholesterol metabolism.
21. Write a note on α-oxidation and ω-oxidation of fatty acids in detail.
22. Define and state the importance of following:
(i) Iodine number
(ii) Reichert Meissl number
 (iii) Saponification number
(iv) Acid number
23. What do you mean by ketone bodies and what is their biochemical significance.
24. Short note on biosynthesis of fatty acids.
25. Explain biochemical importance of cholesterol.
26. Short note on essential fatty acids and their biochemical significance.
CHAPTER 5 & 6
1. Explain following terms with significance:
(a) Apoenzyme
(b) Holoenzyme
2. Differentiate: enzyme and coenzyme.
3. Classify enzyme. Write a note on factors affecting enzyme activity.
4. Give an account on mechanism of enzyme action.
5. Classify enzymes based on the type of reactions they catalyze with relevant
examples and explain clinical significance of enzymes.
6. Explain vitamins as co-enzyme and their significance.
7. Write a note on iso-enzymes.
8. Describe enzymes inhibition with suitable examples [irreversible and reversible
both].
9. Discuss enzymes kinetics in brief and mechanism of action.
10. Discuss the role of enzymes in disease diagnosis and therapeutics.
11. Define enzyme and iso-enzyme and explain iso-enzyme’s clinical diagnosis.
12. Explain metal and vitamins as co-enzymes and their significance.
13. Define:
(i) Cofactor
(ii) Co-enzymes
CHAPTER 7
1. Describe absorption, transport and storage of iron on body.
2. Discuss biochemical role and mechanism of regulation of blood calcium.
3. Write a note on water and mineral metabolism.
4. Short note on mineral metabolism.
5. Explain different mechanism to maintain acid base balance in body.