EXPERIMENT NO.

Aim: To show experimentalily that carbon dloxide is

given out during respiration.
APPARATUS AND MATERIAL
bent at two places at
REQUIRED-A conicai flask, a small test tube, beaker, thread. cork, delivery tuoe
a
right angles in the same direction, some
solution, water, etc. germinating seeds, potassium hydroXIde
THEORY-Respiration is an oxidising and energy liberating
out carbon dioxide on Oxidation of food materials. phenomenon involving the intake of oxygen and giving
solution absorbs carbon dioxide. The Respiration is a basic need of all organisms. Potassium
reactions occurring are as hydroxide
given below:
CgH1208 602 Respiration
Glucose 6CO2 6HO+ Energy
Oxygen Carbon dioxide Water
2KOH +
CO2
Potassiumhydroxide Carbon dioxide K2CO3 H2O
Potassiunm carbonate Water
Dry seeds are living but they are in a state of
seeds start dormancy. After the absorption of water their
respirating and germinating. dormancy is broken. The
PROCEDURE Diagram
Put some
germinated seeds in a conical flask.
Suspend a small test tube containing 20 per Cork
cent potasssium Delivery tube
hydroxide solution with the
help of a thread into the flask while
cork inserting a
having delivery tube bent twice at right
a

angles at two places in the same direction. The Coloured

outer end of the
delivery tube should dip in Conical flask- water
water contained in KOH solution
a beaker. Place this Germinated
apparatus for some time and
keep on seeds
observing the apparatus, especially the outer
end of the
delivery tube.
OBSERVATION-WNater level in the outer tube
rises ascarbon dioxide produced
during
respiration is absorbed by potassium hydroxide Fig. 7:
Experimental set-up to show that
solution and pressure inside carbon dioxide is produced
the flask during respiration
decreases.
CONCLUsION-Carbon dioxide is evolved during respiration.
PRECAUTIONS
() Germinated seeds should not have
any green parts so that
(i) Apparatus should be air photosynthesis does not take
place.
tight.
(n) Take care that KOH solution
does not spil on the
germinated seeds.

VIVA-VOCE
01. Define respiration.
Ans. 02. Name the two
Respiration is an oxidising and energy types of respirations ?
Ans. () aerobic
liberating phenomenon involving the intake of
respiration and
(i) anaerobic respiration
oxygen and giving out carbon dioxide on
03. Do
oxidation of food materials. the plants respire during day or night?
Energy liberated is Ans. Plants respire all the time
ilised to perform various vital activities. day and night. continuously during