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  • Problema 1

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    Clara Paulina Almanza Trejo
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    A batch culture of Atropa belladonna plant roots was established in an air-driven reactor maintained at 25°C. Mass balance calculations were used to monitor growth since roots could not be removed. After 10 days of culture, 1110g of liquid was drained containing 0.063% glucose and 1.7% NH3. The dry mass of roots produced was calculated to be 78g based on a fresh to dry weight ratio of 14:1. Glucose was determined to be the limiting substrate.

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    A batch culture of Atropa belladonna plant roots was established in an air-driven reactor maintained at 25°C. Mass balance calculations were used to monitor growth since roots could not be removed. After 10 days of culture, 1110g of liquid was drained containing 0.063% glucose and 1.7% NH3. The dry mass of roots produced was calculated to be 78g based on a fresh to dry weight ratio of 14:1. Glucose was determined to be the limiting substrate.

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    © All Rights Reserved
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    46 views1 page

    Problema 1

    Uploaded by

    Clara Paulina Almanza Trejo
    A batch culture of Atropa belladonna plant roots was established in an air-driven reactor maintained at 25°C. Mass balance calculations were used to monitor growth since roots could not be removed. After 10 days of culture, 1110g of liquid was drained containing 0.063% glucose and 1.7% NH3. The dry mass of roots produced was calculated to be 78g based on a fresh to dry weight ratio of 14:1. Glucose was determined to be the limiting substrate.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 1

    Culture of plantroots

    Plant roots can be used to produce valuable chemicals in vitro. A batch culture of Atropa
    belladonna roots is established in an air-driven reactor as shown in Figure B. The culture is
    maintained at 25°C. Because roots cannot be removed during operation of the reactor, it is
    proposed to monitor growth using mass balances.
    min−1. During a 10-day culture period, 1425 g
    of nutrient medium are provided, while 15
    litres of CO2 and 47 litres of O2 are collected
    in the off-gas. After 10 days, 1110 g of liquid
    containing 0.063% glucose and 1.7%
    dissolved NH3 is drained from the vessel. The
    ratio of fresh weight to dry weight for roots
    is known to be 14:1.

    (a) What dry mass of roots is produced in 10


    days?
    (b) Write the reaction equation for growth,
    indicating the approximate chemical formula
    for the roots, 𝐶𝐶𝐻𝐻∝ 𝑂𝑂𝛽𝛽 𝑁𝑁𝛿𝛿 .
    Nutrient medium containing 3% glucose and (c) What is the limiting substrate?
    1.75% NH3 is prepared for the culture; the (d) What is the yield of roots from glucose?
    remainder of the medium can be considered
    water. Air at 25°C and 1 atm pressure is
    sparged into the reactor at a rate of 22 cm3

    Culture of plantroots
    Plant roots can be used to produce valuable chemicals in vitro. A batch culture of Atropa
    belladonna roots is established in an air-driven reactor as shown in Figure B. The culture is
    maintained at 25°C. Because roots cannot be removed during operation of the reactor, it is
    proposed to monitor growth using mass balances
    water. Air at 25°C and 1 atm pressure is
    sparged into the reactor at a rate of 22 cm3
    min−1. During a 10-day culture period, 1425 g
    of nutrient medium are provided, while 15
    litres of CO2 and 47 litres of O2 are collected
    in the off-gas. After 10 days, 1110 g of liquid
    containing 0.063% glucose and 1.7%
    dissolved NH3 is drained from the vessel. The
    ratio of fresh weight to dry weight for roots
    is known to be 14:1.
    (a) What dry mass of roots is produced in 10
    days?
    (b) Write the reaction equation for growth,
    indicating the approximate chemical formula
    for the roots, 𝐶𝐶𝐻𝐻∝ 𝑂𝑂𝛽𝛽 𝑁𝑁𝛿𝛿 .
    Nutrient medium containing 3% glucose and
    1.75% NH3 is prepared for the culture; the (c) What is the limiting substrate?
    remainder of the medium can be considered (d) What is the yield of roots from glucose?

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