385 PT 11-84

I 4851903 0021747 7 _
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International Standard 385/ 1
'NTERNATION"\. ORGANllATlON fOR sr AN [}/lRDI ZATI[}H •• '[JlJlr"AI'Ol1tIAII orr ••HIfJAUHA no CTAH¡¡APTIUAU~I!.OAOI\N'SI\TION INTE:RNATION ••U [}¡ NORI,\A\.ISIITION

Laboratory grassware t- Burettes

Part 1 : General requirements
Vefferie de /8bor8toire - BUfe/tes - Ptlrtie , : slécific8tions généra/es

First edition - 1984-10-15

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BIBLIOTECA

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UDC 542.3: 531.73/ .75 I Ret. No. ISO 385/1-1984 (El

D&8crlptor8 : laboratOlV equ;pment. laboratory olassvva,e, burettes. classificlltions, specificatic>ns, dimenslons.

Price basad on B palias

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 385 PT 1-84 -. 4851903 OD21748 9 Ií

Foreword

ISO IIhestandards
national Inl8rnotloool Org8ni~8tlon
bodies lor Stond8rdr~8tion)¡
1150 membar bodies). The workis a01worldwide
preparrng fedaration 01
IntRrnalional
Slandards is normally carried out through ISO lechn;ql commitlees. Each membe.r
body i,,!ere5ted in a subject 10( which a !echnical committee ha5 been established has
Ihe righl to be repre5entad en lhat committee. Interna~ion81 organizalions, govern-

mental and non-governmental, in liarsen with ISO, also itake parl in the work.
Draft Intemational Standards BdoptBd by !he technical1cornmittees are circulated to
Ihe member bodíes tar approval before their acceplance as International Standards by
Ihe ISO Cauncil. They are approved in accordance wjl~ ISO procedures requiring 81
16,,5175 % approval by Ihe membP.r bodies voting.

In¡emational Standard ISO 385/1 was prepared by Teclinical Commlttee ISO/TC 48,
Laboratory glassware and related apparatus.

It cancels snd replsces ISO Recommendarion R 385-1$4, of which it constitutas a

18chnical revision.

<l> Inlemalional Organization lor Sund ••rdizalion. 1984 I •

Prinlp.d in SwitzerlBnd

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 385 PT 1-84 I 4851903 .0021749 O T
INTERNATIONAL STANDAR D ISO 385/1-1984 (El

Laboratory glassware t- Burettes

Part 1 : General requirements

1 Scope 8nd field of applic8tion elas!! B far the Iower grade.

In neither elass shall the I¡mlt of volumetrlc error exceed the
TI1isparl af ISO 386 provicles generaJ requiremont$ far an inter- smallest scale divillion.
nationally Bcceptabte señes 01 buraltes, adequat~ far general

laboratory purposes. I
Tlle details specified are in COI1
lo rmity with ISO 384.
5 Types of buretta
NOTE - Particular requ;rement8 '01" different lypoB 1f bUTOttaare Thc following two tyP&$01 bUretl8 ara spacified :
",,,.,,mar! 1" ISO 385/2 <inri ISO 385/3.
type I burettes, for whlch no waiting time 15 specif¡ad,
ClasB A and Class B (sea ISO 385/21;
2 References
type 11burettee, for whlch a waltlng time is speclfied,
ISO 384, l.abolatolY g/oJsware - Principies of de~ign and eon-
slruction of v()lufTlu(ríc gla:¡:¡wDru. Class A only (SBA·'SO 386/31.

ISO 38512, Laboratory glassware - Burettes I - Part 2 :

81/mlreS for which no waíting time is specified.
ISO 385/3, Laboratory {Jlassware - Burettesl -
Bareltes for whích ti waitíng time o( 30 s Is sP8cified.
ISO 4787, Laboratory glasswaro - Va/umBtr/e (Jlasswa1'8
Methods for use and testing of capacity.
6 Limits of volumetric error
Part 3 : 6.1 Errors In the delivered volume ahal! not oKceed lhe limils
given In table 1. These limits represent tha maximum permilted
error al any polnt and sIso the maxlmum permi99ible difference
between the errors 8t any two points.

Table 1 - Capaclties, sub·divisions and IImlt. of error

3 Basis 01 adjustment
j;1:±
Cla88
Nominal ±
±
mi 0,01
0,06
1:0,02
mi0,06
0,03
0,02
0,06
0,2 mi
0,1
0,1 B AUmil8
±SmaUest
Clas6
0,05
0,1
0,05
0.2
0,02 so,sreo, 8rr01'
divisian
0,02
0,01
mi capao'IV
3.1 Unit of volume100
26
25
1062
1EiO

whieh the name millilitre (mil may be usad.

Tha unit of voluma shall be tne cubre centlmelr Icm3), for
NOTE - The term mllliHlre(mil Is commonly used 88 $ specfat nama
for lha Cllbic centímetre Icm3l, in 8ccordancf) wilh thb Inl"",atlonal

3.2 Reference temperature .

The standard
SYS1:em referenee temperature,
of unÍlslSII• I.D. tha t .l peratllrEJ ilt

(nominal capacity) IIhall be 20 "e.

which the burette is ¡ntended to deHver Its 1l1inel volume NOTE - If bureUas a•.••requirP.d wlth '''IO.ninalcap8cití.)s und/or slIh-
NOTE - WhCll tno bllrollu is rcqulrctJ ror 1I<iO in 8 count.y which hAS
divisionsOlher than those listed In thfs labia, II 18 recommendsd !ha!
adopted ti slandard reference temp",rature o, 'Xl ·C l/he alternativa they shal I'.Onform wilh Ihe eSAenlial requirumunls 01 thJs l)llfl of
rccommc1UlutJ in 150 384 101" lropical llsol, this lia[ue _ha. bu
ISO 385.
SUbSliluted for 20 'C,

6.2 The reJationship belween limits of error and c¡¡pacity for

4 Volumetric Hccuracy class A buretto$ Is given in onnex A.

ThfJl'oshall bu two elasses of aecuraey : 6.3 The relationship between limlts 01 error and the manl8CUS
cfass A for the hlgher grade; diameter far class A buretteB lB glvan In annex B,

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 180385/1-1984 (E)
385 PT 1-84 I 4851903 0021750 7~

7 Construction 7.4.2 Stol)cock and jel should form en integral part of 011
class A burettel! wi1h Iho jet joined lo the stopcock barrel/see
7.1 Material figure 31 or forming part of thc stopcock key 1$00 figUrA 4).
Class B buretles should preferably have integraljlolts bUI. altor-
8ur8ttBs shaJl be manufactured from glass cf :¡uitable crnÚ"ical
natively. may be filted with other types of control.
and thermal properties. shall be 8&.ree as possib/e from visible
defocls and sha/I be rAilsonably free from inlernal stress. 7.4.3 Stopcot:ks of conventiorllll pallern made entiraly of
91a5&shall have tha barrel aod key fll1e1y ground. proforably to a
tapar of 1J 10. and shall comply with the appropriate nlltional or
7.2 Dimensions international specifiC<Jlion!l fnr Slopcock&.

Burenes shall comply with the dirnc"sional reQlliretj1ents 7.4.4 Stopcocks of otnar deslgns whelher of ¡¡Iass or sultable
!lhown in table 2.
inert pl&;1ics materi~ are illlolNCd provided that they comply
with Ihe requiremenls of 7.4.1.
Table 2 - Dlmenarons

.._.
m rop .---
--.'----
015btM"etle:
- NomiNl'
100
mm
600
mm500
450
850
160
250
0.02mJ
mil".
0.05
600
250
600
200
550
0.2
0.01
0.1
0,1
0,02
0,05
2!X)
JOO
fC8le Sesle
SmaUeat
",In.lenglh
0.01
Oye •••"
I
'''ng~hl!
7.4.6 SuitabJe retainlng
afO illlawed.
davlcas fer components o. stopcocks

"",x.I
nun 7.5 Stopcock leokage
576
A stopcod< of cOtlvcntiunal desi(Jn mada enlirely of glass or in-
~.- tended for use with groaSQ shan be tested fOf feakage with Ihe
lIIXj burette clamped In a vertical positíon, the stopcock free frcm
ii20 grease. the barre! and key watled with water. and the burette
-..1---·.---570 fillAd ¡nillally to the zero line with water. The rate o. lellkage,
with tho kcy in cilhcr of the fully shut-off positions. shall not ex-
82Ó
62<Í
ceed one SCilIe sub·division in Iha case al cJass A buraltas or
t\'110 scale sub·divislons in the case of class B burettes. in
~.~~. 20 mino
871
Otlle/" all-glass stopoocks shall be subjected to similar tesIS with
IhaÍ!' componeot part5 free from grea¡:e anrl Wf!1tecfwith water.
C"tance of Iowost or"dlJlI1iun

r
lille ""'" 101' nt mil'. fiel lo ti
510pcock :
In addítion to this test. !I double-bore :;topoock :;hall not show a
lengrh 01 tube 01 uniform boIe be!ow lowcsl mil'. 50 ratA of leakage greatar than that given ¡¡bova when testad
gradual!on l;no : simitarlv. wjth Ihe buralle empty, the key of the stopcock In tIle
DIst8nce 01 end 01 il1t from undc",lde ot stop- normal delivcry position Rnti Ihe f~lin!J tuoo connP.<;ted \O 11
mil'. 20 rm SlJitab/" graolJ<lled tubA fiHed wilh water to a leve! of 250 mm
cnck: mil'. 50 rpm
abovc thu ZCfO II"e of Lile burclLe.
1) 01 bunHtp_~1'01 pfOyided with a nling device.
If the stopcock Is of such moteria! that ;1 i5 intended for use
7.3 Top of burette
without greasB. It &hall be tesled in a similar manner. The rate
01 leakaga &l1allnot axceed one half o. one scale sub-division In
The top of each burulte. if 110t provlded with a fillin[J di:Mce,
the case of class A bllrffttp_<¡. or onl! f;(".11IA
sub-divislon In Ihe
shall be smoothly finished wilh 8 slrengthening tlange or be.1d.
case of dass B burettes, in 50 mino
and shBn be 8t ñght angJes to the axis ct the burettes. Bu1rottcs
wlh sub-divisions of O,OS mi or less, if not fitled with a lfilling For 11M lellkoge tests. In order to onsure a sutticiently élccurale
stopcoc:k, should preferably be finished with 11cylindrical cup al
determination. precautions !!hall be laken against large
the topo Larger diameter buretles may hR tittp.d wlth a funnel
temperature changes land evaporation) durlng tila pariod o.
test.
top fer special purposes. I
The lenglh of tube of uni.orm boro bctwccn thc zero graduiI- NOTE - The leakage lests specif~d ebO\le ere suit.eble lor spedli-
catlon and ,elereo purposos. For ",.ny Olhr:f f'ur,,,,",,,,, a qoicker laol
may 00 nBce&&a,y.In whfch case a vacuum lelll<age Nrengement 11
tion Une end the cup shall be 8t lee SI 20 mm. I .uitable. Such a lest is nol suit-••bI" tor CpP.cÍfir.alionfM"IIO""S owing lo
NOTE - Tho con.~tr" ••1jon of a fillill9dP.vice, provkJed llllhe lopof Ihe
bUfelle, i6 ouls!de the scope ot thls pan of ISO 385. Ihe difficulty of standardizino en Ihe conditions. Aroy particular quick
te.t epparalus can be colíbwtod by 1ho IU¡O 01 O.,V()flllhuretl"" whose
It snell nol. however. inrerferc wlth thu ¡,cr:llntcy ,"'¡II;I1!fT1f1nlti
1m Ihe leakage ratea have been d9termin&d by means 01 the leakage lesl
borellas.
specificd abolle.

7.4 StopcOGks and similar devices 7.6 Dclivmy jet

7.4.1 Stopcocl(s sI,aU be of 9000 quality construction t~ allow The jet ¡;hall be madc from thick-walled C8piUarv 1uhiog 8f\d. If
smooth end precise control ol olltf1ow and to preven! a rata nf it form~ an integral part of the bureUa, shal! hava no cavitv at
leakage greeter thsn thllt ullowud by 7.6. Ihe ;oin likely to Irap ait- bubbles.

z
,[ ..
".' . J. ~_, ~_"-
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 385 PT ],-84
I 4851903 0021751 9
ISO 385/1-1984 (El
Ií

The jet 5ho1l be solidly monufactured with al smooth and

B.3 length of graduatloh lines
gradual tlllper wilhout any 5udden constrlction at the orifice
Oraduation pattarn I applies to class A burettes in aecordance
which could g/ve risa to a tutbulant outflow. I wilh ISO 385/2, graduation pattem 11I applies to class B
Tha and 01 the jet shaU be finlshecl by ona 01 the following burette, In accordance with ISO 385/2 and graduation
pattern 11 preferably applles to burettes In accordanco with
melhods : I 150 385/3, bul may also be used ler cless B burettes in occord-
11I) smoolhly ground, square with the axis, sllghtly bevelled anco wjth ISO 386/2.

8.3.1 Graduation pattern I

b) smoothly ground, square with Ihe axi and slighlly
bavelled on lhe outside; .
8.3.1.1 The lengdl 01 Ihe short linas sMI! ba approximately,
on the outsida and fira·polished;
cl cut at rlght IIngles to the Iongitudinol
j{lxis 000 fire-
but not less than, 60 % of the circulTlfarance of tha buratte.

8.3.1.2 Tho length of the medium linas shaU be approximately

polished .. I 65 % ofthe circumlerence of the buretta and shall eKtend sym-
metricallv at aach end beyond the end 01 the short lines.
NOTE - Theae M6thod. are Usted In order 01 prelerere.
should not result in constriction, as Indlcllle in the lirst B.3.1.3 The long tinas 8hall axtand completaly ",und tha eir-
A flre-pollshed
sentence 01 Ihlstinish reduces the
sub-clausa, OT danger ChlPPj9
in undueof stress 8Sinindicated
use. but cumferAnce of tha buratte, but a gap, nol eXC8ading 10 % of
in 7.1. the clrcumference, rs pormisslble.

B.3.2 Graduatlon pattern 11

7.7 Dellvery time
8.3.2.1 The length of the short lines shall be nol le85 than
Tha 10 % and not more than 20 % of dlB circumlerence oi the
of thedefivllry
water time ia defiL1ed
menlscu8 Irom asthe
the zero
tima Une
oecupied ~y tl1a deseent
to thalowast gradu- burelte.
ation line_ end
The with
dellvery
tOO time
]et notis in
determinad with the
Itheside
stopcock

l
fuUy opan contacl with of the
8.3.2.2 Tha medjum lines shall be approximatefy 1,5 times as
long as tha short line8 and shall extend symmetrlcally at each
raceiving veaBal. end beyond the end oi the short lines.
The delivery tima determinad in this way shall a within the
apecified limita. In the case of 11 detschable jet, t~e appropriale 8.3.2.3 Tho long linos shall axtend completely round the cir-
01 normal use . cumference of the buratte. but a gap, not exceeding 10 % of
. tha circumlerence. ia permlssible.

7.8 Waiting time 8.3.3 GraduetTon pa«arn 11I

delivery tima shall be maintail19d when the jet is the. posilioni[
The wailing time, if specified, is defined as Ihe pe,iod of time to 8.3.3.1 Tho length of the sl10rl Unes shall be not less thlln
be observed after the slopcoek has been elosed and belore the
10 % ond not more than 20 % o, the circumference 01 the
fino! reoding h09 beso taken.
b\Jrelte.

8.3.3.2 The medium lines shall be approxjmately 1,5 times as

8 Gradu8tlon and figuring
long as the short lines ood shall extend symmetrlcslly 8t each
end beyond the ends of the short lines ..
8.1 Graduation liRes
8.3.3.3 The long lines shall be not less than twice as long as
8.1.1 Grodulltion IInes shall be clean, permanent and uniform the short Irnes and shaU extend symmetrlcallv at e8eh end
bayond tlla'anda 01 the short and medlum lines.

I1nes 01 thickness not exceeding 0,3 mm. I

11.1.2 AU graduanon Iins9 shall lie in planes lit T!ght angles lo 8.4 Sequence of graduation Unes fsee figure 11
Ihe longitudinal !lxis of tho bure1le.
8.4.1 . On burettes in which the valume equivalent 01 the
8.2 Spaclng ot graduation Unes smallest scale division iB 0,01 mi or 0,1 mi :

al every tenlh graduation fina &hall ba a long Une;

8.2,1 There sIlall be no obvious irregularity in t~e spacing of
the graduation lines. b) there shall be a medium line midway belween two oon-
sectJtive long lines;

8.2.2 The the

Bueh that limit!! on lenglhs
scale the spacing of graduiltion
are within tha lim ¡Iines shull I.>e
ts allowed in cl there 6hall be four skort lines betwaan consecutiva
tabla 2. medium and long linos.

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 ISO 386/1-1984 (El
385 PT 1-84 I 4851903 002~7S2 011

cJ thara shaU be one short line between two consecutiva

smaJlest scale dlvíslon Js0.02 mi or 0,2 mi : medium fines or between oonsecutíve medium and long
lines.

IIJ evary lifth graduation line I!haUbe 11 long Une;

8.42 On burenes in which the voIume equivalent 1',the
b' duna &hallbe four short lines between two consecutiva
long tines.
8.4.3 On bureues In which Ihe \lo/ume equivalent of the
smalla8t 8cale dlvislon 150.05 mi :
8.6 Position of graduat!on Unes (sea figure 21
8.5.1 On bureltes graduated according to pllttarn l. the anda
Ipreferably the right-hand end) af the short graduatlon IInes
sholl lie on an imaginarv vertical line down Iha centre of the
front of 'he burette, me lines thernaelves should proferabJy ex-
tend te ,helaft when the burette is víewed from the front In the
posillon of normal use.

8.6.2 On burettes graduatad according to pattern 1101' 111,the

e) every 16nth gradualion IIne shalt be a long IIne;
b' there shall be four medium lines equally spacei:l 00-
tween two consecutiv8 long lines;
mid-points of ,he short and medium graduation lines shall &e en
an imaginary ver1iC¿\1line down the centre of (he front 01 the
oorette, when tlle buretle is viewed 110mtha Iront in the pos-
itíon 01 normal use.

8.4.1

11Ila". B.3.3)
oo 110" 11
~I
-::::::-

I ~0,1
!jIU
I -I-==-
8.4.2
8.4.3

LiJ0.02
IIJo,os
I
0,2
=:¡
S.quenc8 of lin••• In Beco.dance with

tI1

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 385 PT ],-84 -.. _~ 4851903·· 0021753 2 __
ISO 385/1-1984 (E)

GrBduBtlon p.ttllm I Graduation pAUflm 11 Graduation paU.rn 111

Figur~ 2 - Posillon o, graduadon IInes

8.6 Figuring of graduatlon IIne5 10 Inscriptions

10.1 The following Inscriptlons shaq be marked on cach
burette :

8) the symbol "cm3" or the svmbol "mi" 10 indicate the

8.6.1
top, at AIIburBttes
the intervalsshall
shownbe figured
In tabledownwards
3. froiI zero allha
Table 3 - Figuring of graduatlon IInes unit In terms of which ,he burstte Is grllduated;
I
SmeUest SCAI. dlvlsion mi 0,01 0,02 0,05 I 0,1
bl tho Inscription "20 °C" 10 Indicate the referenee
lemperaturo;
Figurad I'It every mi 0,5 i,
NOTE- Whuro,cl<captionally,the referencelemperalure 19 Xl ·C,
Ihis va!1JIj 811811
b8 subsliwled ,O( 20 ·C.

8.6.2 Figures sh!!" be placed immedialalv above lhe long 11n63 el tha leuers "Ex" to Indlcate Ihat the burette has been 00-
to wh/ch they refer ond sfightly 10 the right of tl~e end o, tha ¡USled to defiver ilS indicated capacity;
adjacent shorter Unas. In the case of graduation pattern 111,tha
dI the inscrlptlon "A" ar inscriptlon "B" to IOOicatethe
. figures may ba placad stightlV to the right of the epd of tha lina cless of eccurocy for which 1he bUrelte has ooen adjusted;
to which !hay refer, in such a way that an extension 01 the lino
would bisacl Ihem. e) the maker's ¡¡odIar v8fldor's nemo or mark;
f} !he walti"g time. if specified, in the form:
9 Setting of the meniscus "Ex + 30 s".

Setting of tha maníacus shall be cerried out us~g ona of Ihe
errors, the Barna method of &fitting&hallbe usad or both zaro
and end rsadings.
two methods deleitad below. In order lo mfni1ize possib!a
a) The meniscus shllq ba set so that the pl8n~ of the upper
edge of the graduatlan lino 15harlzontally tangantlal10 the
118maplane.
lowest point 01 the manlscus, the line 01 8i9h¡ being in Ihe
bl Tha meniscus shall be oot 80 thal the plana of the
centre 01 the graduation lina is horizontally tangenlial to thB
IO\'V'estpoint of the menlscus, The OVil sh'all be ralsed
10wards Ihe plana and shall observe Ihe front and back por-
tions 01 tha l;ne apparently maating the /owest polnt
slmultaneously.
10.2 The following additional inscriptlons shall ba mer1<eden
class A burettes iotended for offlCialverification or certification
If raquired by legal metr%gy; thay should preferably be
marked on other CIaIlSA burettes eOO may 1IIso be used. if
desired, on class B burBttes :
al on burettes, the jet of whlcn forms part 01 tha stepoock
key (soe figure 41, an Identification number whlch shall be
markod on the burette tuba and repeated on the handra of
the stopcock;
NOTE . - In lhe C8SIJ of burettM, Ihe Jet 01which Is Joinadto Ihe
slopcock barre/, lueh identlficalion ¡. unnecossary, boceuse the
deliverytime cennot be InIluenced by the slopcock boto.
b) the dellvery time, In seconds;

-, .".-- ..
5
.•
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 385 PT ],-84 I 4851903 OD2175~

ISO 385/1-1984 (El

el exeept for b\Jrettes milde from soda·lime glass. an
Identitication of the typa ot glass usad. This requirem~nt vvill
be sBtisfred bVthe m&nlJfaeturer's name or trade-mar~ if tha
coefficient of expansion is publi$hed in his cat810gur
10.3 Tha 1m' 01 volumetrlc error according to table 1 may be
marked on 80 burottes, for B)(8mple by the Ins¿rlption
"± ... mlu•
11 Visibllity of graduation Unes. figures and
inscriptions
11.1 AO figures and Inscrlptions stwll be af such Bize and
form as to be clearlv legible under normal conditions 01 use.
11.2 Al! grdduation lines. figures and inscrlptions shall be
dearly visible Bnd permanent.

Figure J - Jet jolned to stopcock barrel Figure 4 - Jet tormino part 01 stopcock kev

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 I
••••
II ---
I
L

385 PT 1.-84
4851903 0021.755 b r- ISO 385/1·1984 (El

Annex A
I
Relationshlp between limits of error and capacity

for crass A bure/rtes as required by sub-clause 4.:0f

.•...
ISO 384

o
LO

.- Ito >-
'0
(.)
lO
~
.•..
E

o f

~ ~ ~
o~ d 0-
IW :+ "lIl1all1(( V SSG(:J ~ JWJa :¡!JIOW~CII\ 'o I¡WIl

-
,
 - I

• ISO385/H984 (El I 385 PT 1-84 I 4851903 0021? 5 b 8 .-

Annex B

Nomograph indicating the r~lationship between limits 01 error and meniscus

diameter for class A burettes as required by sub-clause 4.7 of ISO 384

-,. - . .-0\~1cf'". I-'---;;I~

I I
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Fri Oct 21 12:08:01 1994 I