Abs Protein pada Ekstrak kasar Enzim Bromelin dari Batang, Bonggol, Buah, Kulit Nanas

No. Nama pH Ulangan

Sampel U1 U2 U3
1. Batang 6.5 0.312 0.332 0.342
2. Kulit 0.421 0.400 0.342
3. Buah 0.540 0.551 0493
4. Bonggol 0.409 0.402 0.472

No. Nama pH Ulangan Rata-rata

Sampel U1 U2 U3
1. Batang 6.5 1.4393 1.5651 1.6280 1.5441
2. Kulit 2.1252 1.9930 1.6280 1.9154
3. Buah 2.8742 2.9433 2.5783 2.2112
4. Bonggol 2.0056 2.0497 2.0560 2.0371

y = ax + b
dimana y = nilai absorbans
y= 0.1589x+ 0.0833
R2= 0.9937
Batang
U1.y = 0.312
0.312 = 0.1589x+ 0.0833
x= 1.4393
U2.y = 0.332
0.332 = 0.1589x+ 0.0833
x= 1.5651
U3.y = 0.342
0.342 = 0.1589x+ 0.0833
x= 1.6280

Kulit
U1.y = 0.421
0.421 = 0.1589x+ 0.0833
x= 2.1252
U2.y = 0.400
0.400 = 0.1589x+ 0.0833
x= 1.9930
U3.y = 0.342
0.342 = 0.1589x+ 0.0833
x= 1.6280
Buah
U1.y = 0.540
0.540 = 0.1589x+ 0.0833
x= 2.8742
U2.y = 0.551
0.551= 0.1589x+ 0.0833
x= 2.9433
U3.y = 0.493
0.493 = 0.1589x+ 0.0833
x= 2.5783

Bonggol
U1.y = 0.409
0.409 = 0.1589x+ 0.0833
x= 2.0056
U2.y = 0.402
0.402 = 0.1589x+ 0.0833
x= 2.0497
U3.y = 0.472
0.472 = 0.1589x+ 0.0833
x= 2.0560

Kadar Protein
No. Nama pH Ulangan Rata-rata
Sampel U1 U2 U3
1. Batang 6.5
2. Kulit
3. Buah
4. Bonggol

% Protein = W x FC x 100 %
Volume sampel(ml) x1000
y = ax + b

W = y- a
b
y = nilai absorbans
Fc = Faktor Pengenceran
Batang
U1 % Protein = 1.4393 x 10 x 100 %
0.5 x1000
% Protein = 2.8786

U2 % Protein = 1.5651 x 10 x 100 %

0.5 x1000
% Protein = 3.1302

U3 % Protein = 1.6280 x 10 x 100 %

0.5 x1000
% Protein = 3.256
Kulit
U1 % Protein = 2.1252 x 10 x 100 %
0.5 x1000
% Protein = 4.2504

U2 % Protein = 1.9930 x 10 x 100 %

0.5 x1000
% Protein = 3.986

U3 % Protein = 1.6280 x 10 x 100 %

0.5 x1000
% Protein = 3.256

Buah
U1 % Protein = 2.8742x 10 x 100 %
0.5 x1000
% Protein = 5.7484

U2 % Protein = 2.9433 x 10 x 100 %

0.5 x1000
% Protein = 5.8866

U3 % Protein = 2.5783 x 10 x 100 %

0.5 x1000
% Protein = 5.1566

Bonggol
U1 % Protein = 2.0056 x 10 x 100 %
0.5 x1000
% Protein = 4.0112

U2 % Protein = 2.0497 x 10 x 100 %

0.5 x1000
% Protein = 4.0994

U3 % Protein = 2.0560 x 10 x 100 %

0.5 x1000
% Protein = 4.112