Delta Delta GMO Ratio

Plant
Plant GMO Cq Cq ddCq 2ddCt
Food Cq Cq Plant GMO
100%
GMO 23 27 0 0 0 1.0000
Non-
GMO 24 39 -1 -12 -11 0.0004
Produc
tA 22 30 1 -3 -4 0.0625
Produc
tB 25 29 -2 -2 0 1.0000
Food Ct plant Ct GMO dCt plant dCt GMO ddGMO 2ddCt
control GMO 23 27 0 0 0 1.00
proba n-GMO 24 39 -1 -12.0000 -11 0.00049
proba A 22 30 1 -3 -4 0.06250
proba B 25 29 -2 -2 0 1.00

2^(cont- 2^(contr-prob)
Food Ct plant Ct GMO prob) 35S PSII R %
control GMO 23 27 1 1 1 100
proba n-GMO 24 39 0.000244 0.5 0.000488 0.048828125
proba A 22 30 0.125 2 0.0625 6.25
proba B 25 29 0.25 0.25 1 100

40
8 14.4 10^8 14.4
35 f(x) = − 3.275 x + 40.45
7 17.6 10^7 17.6
R² = 0.999528499435586
6 20.7 10^6 20.5 30
5 24 10^5 24
25
No.de cycle

4 27.3 10^4 27.2

3 30.3 10^3 30.2 20
2 34 10^2 34 15
1 37.4 10^1 37.3
10
5
0
0 1 2 3 4 5 6
Log (copies ADN/uL)
5
0
0 1 2 3 4 5 6
Log (copies ADN/uL)
 Ratio

ΔCt (control−proba )
%
( 2tinta )
R= ΔCt ( control−proba )
100.00 ( 2ref )
1
0.04
Amplificare=10
( −
panta )
6.30
1

Eficienta=[ 10
100.00
%
( −
panta )] −1
100.00
0.04882813
6.25
100

+ 40.45
99435586

3 4 5 6 7 8 9
Log (copies ADN/uL)
3 4 5 6 7 8 9
Log (copies ADN/uL)