Laboratory Work Report Date : 17th May 2018

General Biochemistry Time : 15.00 – 18.00

WIB
Lecturer : Puspa Julistia
P
Assistant : Imelia Dewi
: Suci Hermita

DETERMINING BLOOD GLUCOSE CONCENTRATION

Group 16
Darryl Yee Zhi Heng
(B04178009)
Roshini Muniandy
(B04178014)
Lai Yoke Ping
(B04178022)
Natasha Anne Binti Mohammed Noor Azam
(B40178034)
 DEPARTMENT OF BIOCHEMISTRY
FACULTY OF MATHEMATICS AND NATURAL SCIENCES
BOGOR AGRICULTURAL UNIVERSITY
2018
INTRODUCTION

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METHODOLOGY

Place and Time for Practicum

The practicum was conducted in the biochemistry laboratory of FMIPA of

Bogor Agricultural University. The practicum was held on Thursday, 17 May
2018. The time given for the practicum was from 3.00 pm – 6.00 pm.

Apparatus and Materials

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Procedure

Determination of Vitamin C concentration

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 RESULTS AND DISCUSSION

Table 1 Concentration of blood glucose

Test Ameasured (Au) Acorrected (Au) [Glucose] (mg/δL)
Aquadest 0.103 0.000 0.00

Cstandard 2.693 2.590 1000.00

Csample 0.276 0.173 66.80

Calculations:

Acorrected for aquadest=0.103−0.103

Acorrected for aquadest=0.000 Au
Acorrected for standard=2.693−0.103
Acorrected for standard=2.590 Au
Acorrected for sample=0.276−0.103
Acorrected for sample=0.173 Au

Using the formula,

x
[ glucose ] = 2 x C standard x FP
x1

C standard=100mg /δL

0
[ glucoseaquadest ]= 2.590 x 100 x 10
[ glucoseaquadest ]=0.00 mg/δL
 2.590
[ glucosestandard ]= 2.590 x 100 x 10
[ glucosestandard ]=1000.00 mg/δL
0.173
[ glucosesample ]= 2.590 x 100 x 10
[ glucosesample ]=66.80 mg/δL
In this experiment, we conducted a quantitative analysis to calculate the
concentration of blood glucose using the spectrophotometer (Hussain and Sadozai
2012). From the observation, it was found that the Ameasured for aquadest,
Cstandard, Csample are 0.103Au, 2.693Au and 0.276Au respectively. Using the
aquadest as a guideline, we measured the A corrected for them which are 0.000Au,
2.590Au and 0.173Au respectively. With this, we could find the concentration of
blood glucose which are 0.00mg/δL, 1000.00mg/δL and 66.80mg/δL respectively.
When compared to literature, the blood glucose concentration in the sample is
slightly higher than the normal level for cow which is 55-65mg/δL (Cozzi et al.
2011).

CONCLUSION

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REFERENCES

Hussain Z and Sadozai SK. 2012. A novel Spectrophotometric method for the
trace analysis of glucose. The Journal of Pharmacy. 44(201112):4731-
4733.
Cozzi G, Ravarotto L, Gottardo F, Stefani AL, Contiero B, Moro L, Brscic M, and
Dalvit P. 2013. Reference values for blood parameters in Holstein dairy
cows: effects of parity, stage of lactation, and season of production.
Journal of Dairy Science. 94(8): 3895-3901.