CHEM 132.

2 – BIOCHEMISTRY (LABORATORY)
LABORATORY REPORT

Name John Allan T. Pasana______ Date Submitted: _Dec 28, 2020_______

Lab Schedule: _MTh at 10:00-1:00_____________ Date Performed: _Dec 28, 2020_______

Introduction: Safety Precautions and the Chemical Laboratory

I. OBJECTIVES

 Familiarize the different laboratory apparatus and the specific functions they play.
 Know the appropriate usage and handling of the different apparatus to avoid dangers and hazards in the chemical
laboratory.
 Understand how to carry out a basic experiment utilizing common laboratory apparatus.

Results Inference
1. Measuring Volume When it comes to measuring volume, it is very essential
that we check the laboratory apparatus for any cracks,
a. Using Pipet ____7.5 mL___ stains and damages in order for it not to negatively
affect our results. In this part of the experiment, two
b. Using graduated cylinder ___100 mL____ apparatus (pipet and graduated cylinder) were used.
The first one is used for transferring or measuring out
small quantities of liquid while the second one is used to
measure volume of liquids, chemicals and other
solutions.
2. Measuring Weight When measuring weight, warm up the analytical balance
for 30 minutes before using it. For solid substances,
a. Tare weight (wt. of container) __2.2461 g____ don’t directly weigh the substance itself, weigh first the
weight of the weighing boat. After weighing the weight of
b. Gross weight (wt. of container w/ sand) __4.4840 g___ the weighing boat, you may then add the desired the
amount of solid substance you need. If you wish to
c. Net weight (wt. of sand) _2.2379 g___ utilize the top loading balance (although it’s not as
accurate as the analytical balance), follow the same
procedure.
3. Precipitation When ferric chloride reacted with sodium hydroxide, it
produced ferric hydroxide. The yellowish-brown color
a. Color of ferric chloride (FeCl3) solution _Yellow-Brown solution turned to a brown color precipitate. Through
this, we can really see how the color changes due to the
b. Color of sodium hydroxide (NaOH) solution _Colorless___ reaction of two substances.

c. Color precipitate ___Brown____

4. Decantation In the process of decantation, the weight of the
evaporating dish itself was first recorded since it will be
 a. Color of supernatant liquid ___Light Pink__ utilized as the receiving container for both decantation
and filtration processes. Moreover, the supernatant
liquid of one of the solution of part C was decanted with
the aid of a stirring rod which then resulted in a light pink
color.
5. Filtration In this process of filtration, a filter paper was utilized by
folding it and shaping it into a cone. It was then weighed
a. Weight of filter paper ___1.2519 g__ in the analytical balance to measure its weight. Next in
order to filter the solutions with the aid of a funnel, tripod
b. Wt. of filter and precipitate (after drying) ___2.3090 g__ and a clay triangle, it was also moistened with distilled
water (also the funnel). After that, the evaporating dish
c. Weight of precipitate ____1.0571 g_ was placed under to receive the filtrate. After draining
the liquids, dry the filter paper and the precipitate and
d. Color of filtrate _Clear/Colorless were then weighed. The filtrate, on the other hand, was
set aside to be used in the evaporation process.
6. Evaporation In the process of evaporation, the evaporating dish
containing the solution was placed on the burner,
a. Weight of evaporating dish __56.8255 g_ supported with a tripod and wire gauze to recover the
sodium chloride through evaporating the water from the
b. Wt. of evap. dish and residue (after drying) _58.7243 g__ solution. After all the liquid has evaporated, solid
substances remained on the evaporating dish. It was
c. Weight of residue __1.8988 g___ then cooled to room temperature and weighed. Lastly, I
noticed that the residue has a texture similar to powder
d. Color of residue ____White____ and has a white color.

e. Texture of residue ___Powdery_