CHEM 4581

Spring 2008
Biochemistry Laboratory I

HOMEWORK #3

Name: Date: February 4, 2008

No late homework accepted.

1. What is the length of bases in yeast tRNAPhe? (Hint: Search the Protein Data Bank.)
(10 points)

2. Describe the covalent differences between DNA and RNA. (15 points)
3. What are the roles of each of the three types of RNA? (15 points)

4. What impact does urea have on biological macromolecules? (10 points)

5. Generate a ribbon diagram of the 3D structure of yeast tRNAPhe. Use RasMol or Protein
Explorer to manipulate the PDB file. Attach the figure. (25 points)

6. Generate a "cloverleaf" illustration of yeast tRNAPhe and label the amino acid acceptor
stem, the anticodon stem and loop, the D stem and loop, and the T stem and loop. Attach
the figure. (25 points)