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Biochemistry II (100 Items)
Biochemistry II (100 Items)
Question 4
The overall chemical reaction of aerobic respiration is:
C6H12O6 + 6 O2 → 6 CO2 + 6 H2O + Energy
Please analyze this reaction carefully, and choose the
description which describes it best.
Question 5
The following diagram depicts the tetrahedral active site
intermediate formation during serine protease catalyzed
peptide bond hydrolysis. Aspartate (Asp), histidine (His), and
serine (Ser) are amino acids of the serine protease, which are
A. A close to each other and can interact to catalyze the
B. B breakdown of certain peptide bonds in proteins. The protein
C. C is R'NHCOR in the diagram below. You can see hydrogen
D. D bonds between the enzyme's Asp and His, and also the His is
taking away a hydrogen from the Ser hydroxyl group. Which
Question 3 of the following statements best describes the Ser attack on
Below is the conversion of ubiquinone to ubiquinol. This the peptide bond?
reaction takes place in the inner mitochondrial membrane,
and ubiquinone and ubiquinol are two forms of coenzyme Q
in the electron transport chain. Please analyze the following
reaction carefully, and choose the statement which describes
it best.
A. +1, -1
A. Ser is a strong acid; this is why Ser gives His a proton. B. +4, +2
The result is a stable tetrahedral intermediate. C. +3, 0
D. -2, -1
B. The hydrogen is a strong electrophile, and it attacks
the peptide bond forming a negatively charged Question 8
oxyanion. Use the following structural formula to calculate the
C. The deprotonated Ser is a strong nucleophile, and it oxidation state number of the carbon atom in urea.
attacks the peptide bond forming a negatively
charged oxyanion.
D. Ser is a strong base, and it attacks the peptide bond
forming a negatively charged oxyanion.
Question 6 A. +2
The following diagram depicts lactase catalyzed cleavage of B. -2
lactose. Glu-1749 is the side chain of the active site glutamate C. +4
amino acid in the lactase enzyme. Lactose is a disaccharide; it D. -4
is composed of a galactose and a glucose. Downregulated
lactase expression results in lactose intolerance. Please Question 9
analyze the following diagram carefully, and choose the Fill in the blanks. A simplified outline of the catabolism of
statement which describes it best. proteins, polysaccharides, and fats can be seen below.
Nutrients are ______ when they are converted to acetyl-CoA.
The citric acid cycle produces the ______ form of
nicotinamide adenine dinucleotide, which is _________ by
the electron transport chain.
Question 11
Fill in the blank. Sucrose, the common table sugar, is a
disaccharide, which is formed from glucose and fructose
monosaccharides. The structural formula of sucrose is shown
below. The glycosidic linkage between glucose and fructose A. A
makes sucrose a(n) ______. B. B
C. C
D. D
Question 14
Fill in the blank. The diagram of protein synthesis can be seen
below. The addition of new amino acids during protein
A. Ether synthesis is a(n) _____ reaction.
B. Ester
C. Acetal
D. Hemiacetal
Question 12
Fill in the blank. Sucrose, the common table sugar, is a
disaccharide, which is formed from glucose and fructose
monosaccharides. The structural formula of sucrose is shown
below. The formation of sucrose from glucose and fructose is
a(n) _____ reaction.
A. Substitution
A. Substitution
B. Elimination
B. Elimination
C. Condensation
C. Condensation
D. Redox
D. Redox
Question 15
Question 13 At the initial step of β-oxidation, acyl-CoA (the general
Glyceraldehyde-3-phosphate (G3P) is an intermediate of structural formula is shown below) is converted in an
glycolysis. The formula of G3P is shown below. G3P is elimination reaction. What is the product of this reaction?
converted to the next intermediate in a redox reaction
catalyzed by a dehydrogenase enzyme. What is the product
of this reaction?
C. Choline. Choline is linked to the phosphate group in
phospholipids.
D. An amino acid. Amino acids are the building blocks
of proteins.
Question 19
What does the structural formula below show, and what is its
function?
A. A
B. B
C. C
D. D
A. N-acetylglucosamine. N-acetylglucosamine is a
building block of bacterial cell wall.
B. A nucleotide. Nucleotides are the building blocks of
Question 16 nucleic acids.
Fill in the blank. Glycolysis produces ATP via _____ . C. An amino acid. Amino acids are the building blocks
of proteins.
D. Uracyl. Uracyl is a building block of RNA.
A. Substrate level phosphorylation
B. Redox reaction
Question 20
C. Oxidation
What does the structural formula below show, and what is its
D. Isomerization
function?
Question 17
What does the structural formula below show, and what is its
function?
A. 1
B. 2
C. 3
D. 4
Question 26
A. 2 How many chiral carbon atoms can be found in the following
B. 3 organic molecule?
C. 4
D. 5
Question 23
How many chiral carbon atoms can be found in the following
organic molecule?
A. 1
B. 3
C. 5
D. 6
Question 27
Fill in the blank. Triglycerides ______ chiral carbon atoms.
A. Always have 1
A. 1
B. Always have 2
B. 2
C. Always have 3
C. 3
D. Do not always have
D. None
Question 24 Question 28
How many chiral carbon atoms can be found in the following The following diagram depicts energy level changes during
organic molecule? chemical reactions. One of the lines represents a reaction
without a catalyst. What can be the relationship of the two
energy profiles on the diagram?
A. 1
B. 2
C. 3
D. None A. The reaction without a catalyst must be the red line.
The green line cannot be the same reaction
Question 25 catalyzed by an enzyme, because the energy levels
How many chiral carbon atoms can be found in the following of the green and red products are different.
organic molecule? B. The red line is the enzyme catalyzed reaction,
because enzymes speed up reactions and produce
higher energy level products. The green line is the
reaction without a catalyst.
C. The red and green lines represent two different D. Both the green and the red lines represent exergonic
reactions; it is unclear if any of these reactions are reactions.
enzyme catalyzed or not.
D. Both the red and green lines represent enzyme Question 31
catalyzed reactions; the green and red energy peaks You are a scientist and you want to set up a protein
represent the breaking of the chemical bonds in the phosphorylation reaction using PKA. You purchased PKA from
substrates. a biotech company, and the datasheet tells you that PKA
requires ATP, cAMP and Mg2+. What could be the role of
Question 29 these reaction mixture ingredients?
The following diagram depicts energy level changes during
chemical reactions. One of the lines represents a reaction A. ATP and cAMP are coenzymes; Mg 2+is a cofactor.
without a catalyst. Could the other line be the energy profile
of an enzyme catalyzed reaction? B. ATP is a substrate; cAMP and Mg 2+ are cofactors.
Question 32
You are a scientist and you would like to set up a carbon
fixation reaction in the test tube. You would like to make
glyceraldehyde-3-phosphate [G3P] from carbon dioxide; you
would like to mirror the Calvin-Benson cycle. What should
A. The red and green lines represent two different you consider, when you design your experiment?
reactions; it is unclear if any of these reactions are
enzyme catalyzed or not. A. This will be an exergonic reaction, which will
B. Both the red and green lines represent enzyme produce ATP and other possible high-energy
catalyzed reactions; the green and red energy peaks molecules as well.
represent the breaking of the chemical bonds in the B. This will be an exergonic reaction; thus, cooling will
substrates. be necessary if you would like to avoid the high-
C. The green and the red lines may represent the same energy G3P-driven explosion.
reaction; the red is without a catalyst, and the green C. This will be an endergonic reaction; thus, you will
is enzyme catalyzed. need an enzyme to catalyze it.
D. The green and the red line represent different D. This will be an endergonic reaction; thus, you will
reactions, because the green and red activation need high energy compounds that can provide the
energies are different. energy, which will be stored in G3P.
Question 30 Question 33
The following diagram depicts energy level changes during Fill in the blank. Bile acids emulsify_____ and expose them for
chemical reactions. Which line represents an endergonic, and _______ in the small intestine.
which one represents an exergonic reaction?
A. Cholesterol, the pancreatic hydrolase
B. Chylomicrons, apolipoprotein B-100
C. LDL, LDL receptor
D. Triglycerides, the pancreatic lipase
Question 34
Fill in the blank. Bile acid can emulsify fats, because it is
_____.
A. Hydrophilic
A. The green line represents an endergonic reaction; B. Amphipathic
the red line represents an exergonic reaction. C. Lipophilic
D. Hydrophobic
B. The red line represents an endergonic reaction; the
green line represents an exergonic reaction. Question 35
C. Both the green and the red lines represent Fill in the blank. Triglycerides combine with bile to form
endergonic reactions. _____________ and enter the central lacteal of the villi.
D. Succinyl-CoA
A. Low density lipoproteins
B. High density lipoproteins Question 39
C. Chylomicrons Fill in the blanks. ___________ the end product of β-
D. Very low density lipoproteins oxidation, which contributes to ___________ in the first step
of the Szent-Györgyi-Krebs cycle. An increased level of the
Question 36 latter activates fatty acid synthesis through the activation of
β-oxidation breaks down fatty acids to acetyl-CoA, but odd- acetyl-CoA carboxylase.
numbered fatty acids cannot be fully converted into acetyl-
CoA. How are odd-numbered fatty acids metabolized? A. Carbon dioxide is, NADH and FADH2
B. Acetyl-CoA is, oxaloacetic acid
A. Odd-numbered fatty acids contribute to the C. Acetyl-CoA is, citric acid
regulation of the Szent-Györgyi-Krebs cycle's D. Acetyl-CoA and glycerol are, ATP
intermediates through giving rise to succinyl-CoA.
Question 40
B. Odd-numbered fatty acids do not occur naturally, Fill in the blank. Both fatty acid synthesis and catabolism
and there is no metabolic pathway to break them takes place through a series of ___________ reactions.
down fully. If industrially produced odd-numbered
fatty acids are consumed, then the last three carbon A. Elimination
atoms will be excreted as a waste in the form of B. Dehydration synthesis
propionic acid. C. Substitution
C. Eukaryotic cells cannot break down the last three D. Redox
carbon atoms containing propionyl-CoA, but bacteria
in the gastrointestinal tract can. Bacteria will Question 41
produce methane gas from propionyl-CoA, and this Medium chain acyl-CoA dehydrogenase (MCAD) deficiency
will result in gastrointestinal discomfort. affects 1 in 17000 people in the United States. Why should
D. β-oxidation breaks down fatty acids to acetyl-CoA MCAD deficient people avoid prolonged fasting?
and propionyl-CoA. Both acetyl-CoA and propionyl-
CoA can step into the Szent-Györgyi-Krebs cycle, and A. High glucagon level during fasting activates fatty acid
they can be used to generate NADH and FADH2 for oxidation, which cannot be completed due to MCAD
the electron transport chain. deficiency. Fatty acids will be increasingly retained in
the adipose tissue, and this will result in obesity.
Question 37
What is the contribution of fatty acids metabolism to the B. High glucagon levels during fasting activate fatty acid
production of metabolites for biosynthesis and to energy oxidation, which in turn inhibits glucose oxidation. If
production? glucose oxidation cannot be inhibited as the result of
MCAD deficiency, then the brain will not get
A. Metabolites for biosynthesis are produced by fatty sufficient glucose.
acid catabolism; only anabolism makes energy. C. This is a common misconception. MCAD deficient
people are simple lucky, because they can eat fatty
B. Metabolites for biosynthesis are produced by fatty food. Most of the consumed fat is excreted in the
acid anabolism; only catabolism makes energy. form of medium chain fatty acids, and their adipose
tissue cannot accumulate in excess.
C. Metabolites for biosynthesis are produced by fatty D. MCAD deficient people do not have efficient fatty
acid catabolism and anabolism; only catabolism acid catabolism in their brain, because most of the
makes energy. fat energy is lost during the excretion of medium
D. Metabolites for biosynthesis are produced by fatty chain fatty acids.
acid catabolism; both anabolism and catabolism
make energy. Question 42
Fill in the blanks. The transcription factor, __________,
Question 38 regulates both the synthesis and the cellular uptake of
Fill in the blank. The synthesis of__________ is the first step cholesterol. Thus, this transcription factor is directly involved
of fatty acid synthesis; this metabolite inhibits carnitine in _________.
palmitoyltransferase, which controls fatty acid oxidation.
A. SCREBP, artherosclerotic plaque formation
A. Malonyl-CoA B. SCREBP, development of high blood pressure
B. Acetyl-CoA
C. Citric acid C. Sterol, risk level of cardiac arrest
D. Sterol, cardiovascular disease
Question 43
The simplified diagram of the sterol biosynthesis is shown
A. A
below. What is the name of the growing red-blue structure in
B. B
this pathway?
C. C
D. D
Question 46
The chemical structure of a free fatty acid is shown below.
How would you characterize this fatty acid?
A. 18:4 ω-3
B. 18:3 ω-4
C. 18:3 ω-6
D. 18:4 ω-6
Question 47
The chemical structure of a free fatty acid is shown below.
How would you characterize this fatty acid?
A. 18:9 ω-2
B. 18:6 ω-9
C. 18:2 ω-6
D. 18:2 ω-6
A. Mevalonate
B. Lanosterol
Question 48
C. HMG-CoA
You can see the space-filling models of two fatty acids below
D. Isoprene
(1 and 2). Which fatty acid is essential, and which one is non-
essential for humans?
Question 44
Which of the following is a five-carbon organic compound and
is a precursor of terpenoid biosynthesis?
A. Mevalonate
B. Lanosterol
C. HMG-CoA
D. Isoprene
Question 45
Which of the following reactions produces mevalonate?
A. 1
B. 2
C. Both
D. None
Question 49
Fill in the blank. Linoleic acid and linolenic acid cannot be
synthesized in human tissues, because ____________.
A. Human cells can synthesize only mono-unsaturated
fatty acids.
B. Humans are diploid organisms; only polyploid
organisms have enough genes for the biosynthesis of
theses fatty acids.
C. The corresponding biosynthetic pathway was lost
during evolution.
D. Human cells cannot synthesize unsaturated fatty
acids. A. 1 is an α anomer, and 2 is a β anomer.
B. 2 is an α anomer, and 1 is a β anomer.
Question 50 C. Both are α anomers.
The following diagram shows the catabolism of the three D. Both are β anomers.
main nutrient groups, including carbohydrates. Which arrow
represents a pathway that is utilized in every cell? Question 53
Which enzyme is a key regulator of glycolysis?
A. Hexokinase
B. Glucose 6-phosphate
C. Phosphofructokinase
D. Pyruvate kinase
Question 54
Fill in the blank. ATP is required for the activity of
phosphofructokinase, but high ATP level inhibits its activity.
This is because ATP _____________.
Question 56
Fill in the blank. Fermentation recycles ______, thus glycolysis
can produce more ATP.
A. FADH2
B. NADP+
C. NAD+
D. AMP
A. Cyclic, NADH
Question 57 B. Cyclic, NADPH
What is the final electron acceptor during fermentation? C. Linear, NADPH
D. Linear, NADH
A. A coenzyme
B. NADH Question 61
C. ATP The following diagram shows an outline of the Calvin-Benson
D. An organic molecule Cycle. Why is this pathway called a "cycle?"
Question 58
58. Fill in the blanks. Louis Pasteur, a French chemist, was the
first to determine that the presence of some microorganism
results in ____fermentation, while other microorganisms
produce _____.
A. Alcoholic, acid
B. Acidic, carbon dioxide
C. Alcoholic, carbon dioxide
D. Ethanol, lactic acid
Question 59
The following diagram shows an outline of cellular
respiration. Why is the Citric Acid Cycle called a "cycle," but
the glycolysis and the electron transport chain is not?
Question 63
Fill in the blank. A eukaryotic cell that lacks ribulose 1,5- What is different in the Szent-Györgyi-Krebs and the Calvin-
bisphosphate is a(n) _________. Benson cycles?
Question 72
Complete the sentence. Ornithine is essential in the urea
cycle, because ______________.
Question 73
What is a common way of regulating amino acid synthesis?
Question 76
What regulates the committed step of the urea cycle?
Question 77
Which statement best describes the metabolism of amino
A. A
acids?
B. B
C. C
A. Glutamate is involved in both amino acid synthesis
D. D
and amino acid breakdown.
B. Glutamate is involved only in the deamination of
Question 75
other amino acids.
What is the biological function of the following pathway?
C. The amino acid catabolism and the amino acid
synthesis pathways do not overlap.
D. Pyridoxal phosphate is a coenzyme for
transamination reactions.
Question 78
Which of the following best describes a transaminases
catalyzed reaction?
Question 79
Which enzymes are used during both the biosynthesis and
the catabolism of amino acid?
A. Transaminases
B. Transcarbamilases
C. Phosphoenolpyrivate kinase and phosphatase
D. Dighydroxiacid dehydratases
Question 80
What is the fate of the carbone skeleton during amino acid
catabolism?
A. It can be stored in fats. C. Szent-Györgyi-Krebs cycle
B. It can be stored in glucose. D. Alanine cycle
C. It can be stored in fats and glucose.
D. It always released as carbon dioxide.
Question 81
Which metabolic pathway has the intermediate which is used Question 87
to start the biosynthesis of all branch chain amino acids? What is the heterocyclic intermediate of the purine
nucleotide interconversion?
A. Glycolysis
B. Calvin-Benson cycle A. AMP
C. Szent-Györgyi-Krebs cycle B. IMP
D. Alanine cycle C. GMP
D. Uric acid
Question 82
Fill in the blank. If the urea cycle does not work efficiently, the Question 88
blood ____ levels will increase. Which nucleotides are used as energy source by the cell?
A. Amino acid A. ATP is the only nucleotide that is used as the source
B. Ammonia of energy in anabolic reactions.
C. Glutamine B. ATP is the most commonly used, but GTP, CTP, and
D. Glutamate UTP are used as well.
C. GTP is used during protein synthesis, but every other
Question 83 anabolic pathway utilizes ATP.
Fill in the blank. High ammonia levels _______. D. ATP is the energy source in anabolic reactions, but
catabolic reactions use GTP, CTP, and UTP.
A. Result from the breakdown of urea in the urea cycle.
Question 89
B. Result in unnecessary amino acid production, and Which metabolite is involved in both the salvage of adenine
excessive protein synthesis. and the biosynthesis of adenosine-5'-monophosphate?
C. Decrease glutamate levels, thus it interferes with
neuronal signal transduction. A. inosine-5'-monophosphate
D. Result from the breakdown of carbamoyl phosphate B. Hypoxanthine
at the end of the urea cycle. C. Ribose-5-phosphate
D. 5-phosphoribosyl-1-ribophosphate
Question 84
Which metabolic pathway has the intermediate which is used Question 90
to start the biosynthesis of all aromatic amino acids? The relative activity of kinases determines the nucleotide
levels in the cell. Which nucleotides have the highest levels?
A. Glycolysis
B. Calvin-Benson cycle A. The NMP levels are highest, because the cell invests
C. Szent-Györgyi-Krebs cycle in NDP and NTP synthesis only as needed.
D. Alanine cycle B. The NMP levels are highest, because the nucleoside
kinases are the most active.
Question 85 C. The NTP levels are highest, because the NDP kinases
Which metabolic pathway has the intermediate which is used are the most active.
to start the biosynthesis of threonine and glutamate? D. The NDP levels are highest, because the NMP
kinases are the most active.
A. Glycolysis
B. Calvin-Benson cycle Question 91
C. Szent-Györgyi-Krebs cycle Fill in the blanks. ___________ is synthesized from glutamine
D. Alanine cycle for pyrimidine biosynthesis, but for amino acid catabolism, it
is derived from ____________ bicarbonate.
Question 86
Which metabolic pathway has the intermediate which is used A. α-ketoglutarate, carbamate or
to start the biosynthesis of histidine? B. α-ketoglutarate, ammonia and
C. Carbamoyl phosphate, carbamate or
A. Pentose phosphate pathway D. Carbamoyl phosphate, ammonia and
B. Calvin-Benson cycle
c. Allopurinol, which is an isomer of hypoxanthine
Question 97
Question 92 Fill in the blank. The biosynthesis of purine and pyrimidine
Fill in the blanks. The __________cycle catabolizes AMP to nucleosides utilize _____, which is an activated sugar.
___________.
a. Adenosine-5'-triphosphate
A. Calvin-Benson, glyceraldehyde-3-phosphate b. 5-Phosphoribosil-1-phosphate
B. Purine nucleotide, fumarate c. 5-Phosphoribosil-1-pyrophosphate
C. Szent-Györgyi-Krebs, fumarate d. Guanosine-5'-triphosphate
D. Citric acid, oxaloacetic acid
Question 98
Question 93 Fill in the blank. De novo nucleotide synthesis results in ____,
The most common genetically inherited SCID affects the which can be reduced to ______ as needed.
enzyme adenosine deaminase. This deficiency results in
which of the following? a. Ribonucleotides, deoxyribonucleotides
b. Deoxyribonucleotides, ribonucleotides
a. dATP accumulation and the general inhibition of c. Inosine-5'-monophosphate, guanine-5'-
dNTP synthesis from NTPs monophosphate
b. dATP accumulation and the general inhibition of RNA d. Uric acid, adenosine-5'-monophosphate
synthesis
c. AMP accumulation and the selective inhibition of Question 99
GMP synthesis Fill in the blank. Vitamin B12 deficiency inhibits DNA
d. AMP accumulation and the selective inhibition of synthesis, because it ____.
IMP synthesis
a. Is the precursor of tetrahydrofolate
Question 94 b. Interferes with dTMP synthesis
Fill in the blank. Glutamine and _____ are building blocks of c. Interferes with dUMP synthesis
pyrimidine base biosynthesis. d. Activates glycine synthesis