Saint Michael’s College of Laguna

Old National Road, City of Binan, Laguna

Institute of Nursing
Activity Number 1

I . Title: Common Laboratory Equipments and Apparatus: Its Description,

Proper Use and Function.
II. Students Name/ Course and Year:
Instructor: Ma’am Nina M. Moleta
III. Objectives: Familiarization and proper use of laboratory equipment's and
apparatus and Safety rules and procedures.
A. To familiarize on the proper use and functions of common Biochemistry
Lab Equipments and Apparatus.
B. To broaden the knowledge of Safety Rules when performing experiments
in Biochemistry Laboratory.
IV. Procedures:
A. List down ten (10) common lab equipments in Biochemistry Laboratory.
Mention its description, proper use and function.
B. List down ten (10) safety rules while working in Biochemistry Laboratory
V. Presentation of Data
Name Apparatus Description Proper Use Function
(with picture)
Beakers
Beakers are Never carry a
another cylindrical beaker by its side.
utensil made up of Pour liquid into the
glass, with a flat beaker; pour slowly Are of varying sizes
bottom and the to avoid splashing and are used to
upper opening the liquid. ... Center hold, heat, or mix
having a the beaker on a substances with the
spout.They are of burner or over an proper measure
varying sizes and open flame to heat
are used to hold, the liquid, if

Nina M. Moleta, LPT, MS

 heat, or mix necessary; don’t fill
substances with the the beaker more
proper measure. that 1/3 when
heating and always
use safety tongs
when handling a
hot beaker.

Test tube
The next very -Test Tube Rack:
common apparatus Never leave a test Test tubes are one
are the test tubes. tube on a counter. of the most
They are usually Always obtain a important
cylindrical pipes test tube rack apparatus as they
made up of glass, before moving test are functional from
with a circular tubes to your lab storing to mixing
opening on one station. reagents in any
side and a rounded -Test Tube Holder: chemical or
bottom on the other This instrument is biological reaction
side only necesary when
heating a test tube.
Never touch a hot
test tube. Always
use test tube
holders.
-Glass Stirrer: Use
a small glass stirrer
to mix solutions in
test tubes and
always wash the
stirrer before and
after using it.
-Never carry more
than one test tube
in each hand!

Microscope
A microscope is a -Do not touch the
popular lab glass part of the
apparatus used to lenses with your
observe things that fingers. Use only Used to magnify
are too tiny to be special lens paper small objects.
observed by the to clean the lenses.
naked human eye. -Always keep your
microscope
covered when not

Nina M. Moleta, LPT, MS

 in use.
-Always carry a
microscope with
both hands. Grasp
the arm with one
hand and place the
other hand under
the base for
support.
Erlenmeyer Flask
Never carry a flask used to contain
by its neck. This liquids and for
used to contain mixing, heating,
An Erlenmeyer liquids and for cooling, incubation,
flask, also known mixing, heating, filtration, storage,
as a conical flask or cooling, incubation, and other liquid-
a titration flask, is a filtration, storage, handling
type of laboratory and other liquid- processes. Their
flask which features handling slanted sides and
a flat bottom, a processes. Their narrow necks allow
conical body, and a slanted sides and the contents to be
cylindrical neck.  narrow necks allow mixed by swirling
the contents to be without the risk of
mixed by swirling spills, which is
without the risk of useful for titrations
spills, which is and for boiling
useful for titrations liquids.
and for boiling
liquids.

Volumetric Flask
Begin with a clean
This is one of the flask. Rinse it well
most important with Distilled water.
equipment of any Use a volumetric It is mostly used in
lab, which is made pipet to deliver the the preparation of
up of glass and is Appropriate volume standard solutions.
calibrated to hold a of concentrated
precise volume of solution to the
liquids at any Volumetric flask.
precise Add distilled water
temperature. to the flask, using a
Funnel or wash
bottle, until the level
reaches the neck of
The flask. Cap and

Nina M. Moleta, LPT, MS

 mix the solutions
thoroughly. At this

Point, add water

more slowly.
Finally, add water
drop by Drop until
the bottom of the
meniscus is even
with the Calibration
mark. Stopper the
flask tightly, invert it
and Shake to
obtain a
homogeneous
solution. Repeat
the Inversion and
shaking process
several times.

Bunsen Burner
Place the Bunsen
burner away from It is used for
any overhead heating and
shelving, sterilization
This apparatus equipment, or light purposes in the
produces a single fixtures by at least various
open flame 12 inches. Remove experiments
all papers, conducted in labs.
notebooks,
combustible
materials, and
excess chemicals
from the area. Tie-
back any long hair,
dangling jewelry, or
loose clothing. Use
correct tubing (butyl
rubber hose).

The measuring
A narrow, cylinder should be
cylindrical container placed on a flat Is a piece of
marked with surface. And should laboratory
horizontal lines to be viewed a level apparatus used to

Nina M. Moleta, LPT, MS

 Measuring Cylinder represent units of as you pour the measure the
measurement and liquid. In. The volume of a liquids,
used to precisely measurement chemicals or
measure the should be taken solutions during the
volume of liquids. from the bottom of lab daily work.
the meniscus as
shown on screen.

Thermometer
Thermometer as Place the The thermometer
many times, certain thermometer in the becomes very
chemical or same spot for about necessary to
biological reactions 40 seconds. measure the
can be carried out Readings will temperature of the
in any particular continue to required solution
temperature range increase and the F before moving
only and so to (or C) symbol will forward with further
proceed, flash during reaction procedure
measurement.
Usually, the
thermometer will
make a beeping
noise when the final
reading is done
(usually about 30
seconds)

Watch Glass
When using the
watch glass to It is normally used
This apparatus is cover reaction to hold solids,
more commonly vessels the glass evaporate liquids,
found in chemistry should always be and heat small
laboratories and is placed with the quantities of
made up of a concave side facing different
concave piece of up so the glass substances as per
glass. nests into the the need of the
reaction vessel. experiment.

Nina M. Moleta, LPT, MS

 Pipettes
a small piece of Apply light pressure Used to dispense
apparatus which to insert the pipette measured volumes
typically consists of into the tip. There is of liquids. Pipettes
a narrow tube into no need for force or most commonly
which fluid is moving from side to work by creating a
drawn by suction side. This would partial vacuum
(as for dispensing only strain your arm above the chamber
or measurement) and damaged the that holds the liquid
and retained by pipette and selectively
closing the upper releasing this
end vacuum to draw up
and dispense
according to the
preferred volume.

B. INCLUDE Eye wash, shower, medicine cabinet.

Safety Guidelines
1. Read through the procedures of each laboratory activity before you start the lab.
So that you are familiar with them.
2. Know how to locate and use all safety equipment in the laboratory, including the
fume hood, emergency shower, first aid kit, fire blanket, fire extinguisher, and
eyewash.Also be sure to locate the nearest exit in case of an emergency.
3. Avoid dangerous behavior in the laboratory.
4. Always conduct your experiments with adult supervision.
5. Wear safety goggles when handling all hazardous chemicals, working with an
open. Flame, or when otherwise instructed.
6. Wear an apron or a smock to protect your clothing in the laboratory when using
chemicals.
7. Tie back long hair, and secure any loose-fitting clothing.
8. Never eat or drink in the laboratory.
9. Wash your hands before and after each activity in the lab.
10. Keep the work area free of any unnecessary items.

V1. Analysis
Science lab equipment allows students to interact directly with the data gathered.
They get a first-hand learning experience by performing various experiments on their
own. Science laboratory equipment refers to the various tools and equipment that are
used by professionals or students working in a laboratory. It’s also refers to various gear
used in a laboratory to perform different tasks. The example of different laboratory

Nina M. Moleta, LPT, MS

equipment used are Bunsen burner, microscopes, calorimeters, reagent bottles,
beakers and many more. Some science lab apparatus are used for weighing objects,
mixing and preparing solutions, and others for cleaning vessels. It is quite essential to
go with the right kind of lab equipment.
VII. Conclusion
Overall, we can call them the basic or common laboratory apparatus we use in a
school lab. Laboratory equipment is required to be properly use. It is important to us to
know the description, functions, and the proper use of the equipment. Many of the
accidents that occur in the laboratory can be attributed to improper use or maintenance
of laboratory equipment so that it is important that we have enough knowledge about
different apparatus. If you don’t have a proper knowledge that how to use the chemical
and laboratory instruments properly, you can come across all sorts of damage.
Biochemistry laboratory safety begins with recognition of chemical hazards and
safety focused attitude, good personal behavior, good housekeeping in the laboratory
work area and storage areas and all the continual practice of good laboratory
techniques.
VIII. Reference Materials
Google:
https://www.labkafe.com/blog/14_20-common-school-science-laboratory-equipment-
and-their-uses.html
http://www.csun.edu/~psk17793/G%20Biology/lab_safety.html
Meriam Webster

Nina M. Moleta, LPT, MS