BACTERIAL GROWTH

(REVIEW)

ENVE 309
Fundamentals of Biological Treatment
2021-2022 Fall

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Growth Rate
X
Growth
Medium

V X, S

Optimum Temp., pH x= biomass/volume

S= substrate/volume
V=volume

When S is superflous, μ=𝜇𝑚𝑎𝑥

time
𝑑𝑋
= 𝜇𝑚𝑎𝑥 𝑋 𝑋 = 𝑋𝑜 𝑒 𝜇 𝑚𝑎𝑥 𝑡
ln 𝑋 = ln 𝑋𝑜 + 𝜇𝑚𝑎𝑥 𝑡
𝑑𝑡
𝜇 = 𝑠𝑝𝑒𝑐𝑖𝑓𝑖𝑐 𝑔𝑟𝑜𝑤𝑡ℎ 𝑟𝑎𝑡𝑒 2
 Growth Yield (Y)

∆𝑋 𝑚𝑎𝑠𝑠 𝑜𝑓 𝑏𝑖𝑜𝑚𝑎𝑠𝑠 𝑝𝑟𝑜𝑑𝑢𝑐𝑒𝑑 𝑋−𝑋𝑜

Y= = =
∆𝑆 𝑚𝑎𝑠𝑠 𝑜𝑓 𝑠𝑢𝑏𝑠𝑡𝑟𝑎𝑡𝑒 𝑢𝑠𝑒𝑑 𝑆𝑜−𝑆

𝑑𝑋
When S 0 𝑌=
𝑑𝑆

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 Substrate Utilization Rate

𝑑𝑆
= 𝑞𝑋 Where; q = specific substrate utiliztion rate (or metabolic quotient)
𝑑𝑡

𝑑𝑋 𝜇𝑋𝑑𝑡 𝜇𝑋𝑑𝑡 𝑑𝑆 𝜇𝑋
𝑌= = 𝑑𝑆 =
𝑌 𝑑𝑡
=
𝑌
𝑑𝑆 𝑑𝑆

𝜇𝑋 𝜇
𝑞𝑋 = 𝑞=
𝑌 𝑌

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 Substrate Utilization follows Enxyme Kinetics

𝑣 𝑚𝑎𝑥 𝑆 Michaelis-Menten for Enzyme Kinetics

𝑣=
𝐾𝑚 + 𝑆
Where; Km = Michaelis-Menten constant

So, 𝑣≡q

𝑞𝑚𝑎𝑥 𝑆
∴ 𝑞= Substituting 𝑞=
𝜇
Where; Ks = Half-Saturation substrate concentration
𝐾𝑠 + 𝑆 𝑌

𝜇𝑚𝑎𝑥 𝜇𝑚𝑎𝑥 𝑆
𝜇 𝑆 𝜇=
= 𝑌 MONOD EQUATION
𝐾𝑠 + 𝑆
𝑌 𝐾𝑠 + 𝑆
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 MONOD GROWTH CURVE

When S=Ks ; μ=μmax/2

μmax/2 Ks= half saturation substrate conc. which indicates substrate affinity

The smaller the Ks value, the higher the substrate affinity of the biomass.
Ks