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  • Dna Experiments

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    SIDDHANT
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    The document describes an experiment to isolate DNA from plant materials like spinach, peas, and papaya. The requirements include plant materials, mortar and pestle, test tubes, liquid detergent, sodium chloride, and papain solution. The theory is that DNA and RNA are types of nucleic acids found in living organisms, with DNA serving as the genetic material containing the blueprint of the organism. The procedure involves grinding plant tissue, adding detergent, salt solution, and papain enzyme, then collecting the precipitated DNA using ethanol and testing with a spectrophotometer. The observation is that adding ethanol causes DNA to precipitate out as white threads that can be seen and measured spectrophotometrically.

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    DNA experiment CLASS 12TH BIO ACTIVITY PDF

    Original Title

    dna experiments

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    The document describes an experiment to isolate DNA from plant materials like spinach, peas, and papaya. The requirements include plant materials, mortar and pestle, test tubes, liquid detergent, sodium chloride, and papain solution. The theory is that DNA and RNA are types of nucleic acids found in living organisms, with DNA serving as the genetic material containing the blueprint of the organism. The procedure involves grinding plant tissue, adding detergent, salt solution, and papain enzyme, then collecting the precipitated DNA using ethanol and testing with a spectrophotometer. The observation is that adding ethanol causes DNA to precipitate out as white threads that can be seen and measured spectrophotometrically.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    11 views3 pages

    Dna Experiments

    Uploaded by

    SIDDHANT
    The document describes an experiment to isolate DNA from plant materials like spinach, peas, and papaya. The requirements include plant materials, mortar and pestle, test tubes, liquid detergent, sodium chloride, and papain solution. The theory is that DNA and RNA are types of nucleic acids found in living organisms, with DNA serving as the genetic material containing the blueprint of the organism. The procedure involves grinding plant tissue, adding detergent, salt solution, and papain enzyme, then collecting the precipitated DNA using ethanol and testing with a spectrophotometer. The observation is that adding ethanol causes DNA to precipitate out as white threads that can be seen and measured spectrophotometrically.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    of 3

    AIM OF THE EXPERIMENT

    iselate_NA emavailabilils plaril _miateuial suthas spnach lau


    n pea Seuds papaya elc

    REQUIREMENT
    lant matexialsuuh as gpinath luauus, gtun pea sude 8 qtun papaya)-
    Mattar and pecte heakeus, test tukes,llquid ditengarit nem- Tedted
    Seciun chlanide diskillkd uaáten, Mia tenduixex papain scluetie
    E aRAya n appl uite,15 dhanal Specl et
    HEORY
    i Reaxynilbenuleicaid DNA) and i lhenulsie aic (rNA) a tun dypes
    enuli aidound in linq ayitnM NA ad as u 4eni
    mtaial in aa etheotqanitm
    nRNA tuugh talsa ada a qnchc malkaial ia sema utuA
    Mest Aunchems_a a mAAinquadapliu,stuuafistal and în sema lasas
    aA tatalyhic meletals
    n nsuunt " htsak hrsugh in Ha4ila e bictechnelsq a th
    huun Hypea ptedueken_e qyakttalls Malited suganisma pfants , animala.
    natuasngnisnastaueughonabinant NA (r bNAAchnelqu
    Retomhiant PNA tehrelagyqunehe enginasing has altud hxuadus
    o inhneauueeoteian_DNA n MhaStQahisnm).
    Suuh DHQanisnaak Lallu gunultall mediled emanisimn (GMos) Jkus
    DNAtchaeloq4 inveluu iselaied DNA m a
    Vasuicds o
    SeLLAand farthaion t w embina:iom_b NA

    Thepaualien rtien
    Aelguit saltselueion sptupatud hy aadinq 10 ml Bquid dedotgaat
    and 10 non-isdiseol sedua_chlekida to O10mdodislled
    atty.
    ig. 8.1. DNA that separates out can be removed by spooling (spool = reel for winding yarn).
    -Meal Lendrizerseluten is Lendutkto
    prepatud b addir
    5 nil e disltllcd ale
    5 Nac selulien ts ptepastecl by dissplve bq nen -tocisecl Sodiun
    chlside in 46 n e dislillkd Ndu.
    dillinq e l thanet nusl h dene ay keping 95 elhanel in plasic
    betle thehurtxs e tigkt

    PRACEAURE
    5 thu planit taken and qsinded in Ha mertlau y.
    tissut udas

    arddinq n l dateuqsnt,salt selution and tted hteugh muslin dlen


    10nl ttiate uns taken, 3-4 m endnizadwa addad and
    Het tuht Aias utltd hq heldinq it Aheluuen hu uo hands lo ni
    tentante
    10
    na shilld eihanel was Caully deuon and t undistuneu
    aabei minute
    lLsing tuglass ted t udas slitnud qurti thteugk inlenface e the
    layks to ila t peteipitate oNA and tt 10ad pla&d in tesl
    ue huith 5 Nac o distled attn
    h quanis e DNA pRLLUnt in hqiuin plavt malial Canhe
    eslinaaledhrieugh spetchtephslemalru
    OBSERVATIAN
    h addilion t cthanet to ths selulion Cauyes DNA to pMipttalion.
    h ANA hs appea a while prtaeTpilate o u in thrttads
    thqlal- Speel

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