Course: PBG 602 (Fundamentals of Plant Biometry)

Lecture: Constructing ANOVA for RCBD and CRD Designs using EXCEL and Determination of
Least Significant Difference Test
Data were recorded on number of grains per panical and panicle length (cm) of 8 rice genotypes.
Our task is to construct ANOVA of these two parameters as RCBD and CRD and estimate LSD for each.
The number of replications both for RCBD and CRD are four.

Grains per Panicle = X1 Genotype Genotype

GENOTYPES R1 R2 R3 R4 Total Means
1 50.2 41.4 36.2 39.8 167.6 41.9
2 41.8 47.2 39.6 46.6 175.2 43.8
3 39.2 37.6 38.8 33.6 149.2 37.3
4 37.8 49.6 35.4 41.8 164.6 41.2
5 35.6 31.4 33.2 29.8 130.0 32.5
6 53.4 50.2 49.6 57.8 211.0 52.8
7 43.8 46.8 41.4 43.6 175.6 43.9
8 50.6 47.8 41.8 46.8 187.0 46.8
Reps Total 352.4 352.0 316.0 339.8 1360.2
No of Reps 4
No of Genotypes 8 C.F. 57817.00
ANOVA as RCBD for Grains per Panicle = X1
SOV d.f. SS MS F-cal Probability
Reps 3 109.2 36.41 2.6 0.0762
Genotypes 7 1024.0 146.28 10.6 0.00001
Error 21 289.7 13.80
Total 31 1423.0 --
C.V. (%) = 8.7
SED of X1 = 2.63 t(0.05) of X1 = 2.08

LSD for X(5%)= 5.5

Panicle length (cm) = X2 Genotype Genotype

GENOTYPE R1 R2 R3 R4 Total Means
1 20.5 20.6 20.5 19.6 81.2 20.3
2 19.5 20.1 19.3 20.1 79.0 19.8
3 19.0 18.5 18.1 19.3 74.9 18.7
4 20.0 20.3 20.6 20.3 81.2 20.3
5 20.0 20.8 20.3 19.9 81.0 20.3
6 19.2 19.5 20.3 19.9 78.9 19.7
7 19.5 20.4 20.7 20.3 80.9 20.2
8 19.7 19.8 20.1 20.5 80.1 20.0
Reps Total 157.4 160.0 159.9 159.9 637.2
No of Reps 4
No of Genotypes 8 C.F. 12688.25
ANOVA as RCBD for Panicle length (cm) = X2
SOV d.f. SS MS F-cal Probability
Reps 3 0.6 0.20 1.1 0.3837
Genotypes 7 8.0 1.14 6.1 0.00058
Error 21 3.9 0.19
Total 31 12.5 --
C.V. (%) = 2.2
SED of X2 = 0.31 t(0.05) of X2 = 2.08

LSD for Y(5%)= 0.64

RCBD ANOVA
Fill in the Blank Spaces
SOV d.f. SS MS F-calculated
Reps 2 --- 80 ---
Genotypes 19 3800 --- ---
Error --- --- 32
Total --- ---

How to Prepare CRD ANOVA from Above???

SOV d.f. SS MS F-cal

Genotypes ?? ?? ?? ???
Error ?? ?? ??
Total ?? ??
 genotypes.
ate LSD for each.

ANOVA as CRD for Grains per Panicle = X1

SOV d.f. SS MS F-cal Probability

Genotypes 7 1024.0 146.3 8.8 0.00002

Error 24 399.0 16.6
Total 31 1423.0 --

ANOVA as CRD for Panicle length = X2 ?????

SOV d.f. SS MS F-cal Probability
Genotypes ? ?? ??? ???? ?????
Error ? ?? ???
Total ? ??