Royce Matthew O.

Castro BS-ChE-2 March 12, 2021

1) Make a tabulation

MCL
Sampling Method
Standard Method
Parameters DAO- and Sample
DAO- of Analysis
PNSDW 16 Preservation
16(A)
(AA)
Multiple Tube
3000 Fermentation
Total
- NDA MPN/ Technique, -
coliform
100 mL Enzyme Substrate
Coliform Test
Multiple Tube
Thermotolera
- - - Fermentation -
nt coliform
Technique,
Pour Plate
Heterotrophi Method, Spread
c Plate - - - Plate Method, -
Count membrane Filter
Method
Electrothermal
0.02 Nitric Acid
Antimony - - Atomic
mg/L Digestion,
Absorption
Manual Hydride
0.01 0.02
Arsenic NDA Generation/ Nitric Acid Digestion
mg/L mg/L
Atomic Digestion
Direct Nitrous Nitric Acid-
0.70
Barium NDA 1.5 mg/L Oxide-Acetylene Hydr5ochloric Acid
mg/L Digestion
Flame Method
Curcumin
2.00 Microwave-Assisted
Boron NDA 2 mg/l Method, Carmine
mg/L Digestion
Method
Electrothermal
Atomic
Nitric Acid
Absiorption
0.003 0.006 Digestion, Nitric
Cadmium NDA Spectrometric,
mg/L mg/L Acid-Hydrochloric
Inductively Acid Digestion
Coupled Plasma
Method
Electrothermal
Nitric Acid-
Atomic
0.05 0.02 Digestion,
Chromium NDA Absorption
mg/L mg/L Microwave-Assisted
Spectrometric Digestion
Method
Cyanide 0.50 NDA 0.14 Titrimetric -
 mg/L mg/L Method,
Colorimetric
Method,
cyanide-
Selective
Electrode
Method
Ion
Chromatography
w/ Chemical
Suppression of
1.50 Preliminary
Fluoride NDA 2 mg/L eluent
mg/L Distillation
Conductivity, Ion-
selective
Electrode
Method
Electrothermal
Nitirc Acid
Atomic
0.01 0.02 Digestion,
Lead NDA Absorption
mg/L mg/L Microwave-Assisted
Spectrometric Digestion
Method
Direct Air-
Acetylene Flame Nitric Acid
0.4 Method, Digestion, Nitric
Manganese NDA 2 mg/L
mg/L Extraction/ Air- Acid-Hydrochloric
Acetylene Flame Acid Digestion
Method
Cold-Vapor
Atomic
Absorption
Spectrometric Nitric Acid
0.001 0.002 Method, Digestion, Nitric
Mercury NDA
mg/L mg/L Inductively Acid-Hydrochloric
Coupled Plasma- Acid Digestion
Mass
Spectrometry
Method
electrochemical
atomic
Nitric Acid
absorption
0.07 Digestion, Nitric
Nickel NDA 0.1 mg/L spectrometric,
mg/L Acid-Hydrochloric
inductively Acid Digestion
couples plasma
method
ion
50.00
Nitrate NDA 14 mg/L chromatrography -
mg/L
with chemical
 suppression of
eluent
conductivity,
single-column ion
chromatography
with direct
conductivity
detection
ion
chromatography
with chemical
suppression of
eluent
3.00
Nitrite - - conductivity, -
mg/L
single-column ion
chromatography
with direct
conductivity
detection
Nitric Acid
Manual Hydride
0.04 0.02 Digestion, Nitric
Selenium NDA Generation/
mg/L mg/L Acid-Hydrochloric
Atomic Digestion Acid Digestion
keep in cool
environment (< = 6
C); for samples that
contain volatile
purge and trap constituents but do
capillary-column not contain residual
gas chlorine, add HCl to
0.01
Benzene NDA 0.1 mg/L chromatographic pH <2.0 (4 drops, 1:1
mg/L HCl); for samples
/ mass
spectrometric that contain
method resdiual chlorine,
add 1000 mg
ascorbic acid/L or
0.008% sodium
thiosulfate)
keep in cool
liquid-liquid environment (< = 6
extraction gas C); for samples that
Benzo(a)pyr 0.0007 chromatographic contain resdiual
NDA 1.5 μg/L
ene mg/L / mass chlorine, add 1000
spectrophotomet mg ascorbic acid/L
ric method or 0.008% sodium
thiosulfate)
 keep in cool
environment (< = 6
C); for samples that
contain volatile
purge and trap constituents but do
capillary-column not contain residual
gas chlorine, add HCl to
Carbon 0.004
- - chromatographic pH <2.0 (4 drops, 1:1
tetrachloride mg/L HCl); for samples
/ mass
spectrometric that contain
method resdiual chlorine,
add 1000 mg
ascorbic acid/L or
0.008% sodium
thiosulfate)
keep in cool
environment (< = 6
C); for samples that
contain volatile
purge and trap constituents but do
capillary-column not contain residual
1,2- gas chlorine, add HCl to
dichlorobenz 1 mg/L - - chromatographic pH <2.0 (4 drops, 1:1
ene / mass HCl); for samples
spectrometric that contain
method resdiual chlorine,
add 1000 mg
ascorbic acid/L or
0.008% sodium
thiosulfate)
keep in cool
environment (< = 6
C); for samples that
contain volatile
purge and trap constituents but do
capillary-column not contain residual
1,4- gas chlorine, add HCl to
0.3
dichlorobenz - - chromatographic pH <2.0 (4 drops, 1:1
mg/L HCl); for samples
ene / mass
spectrometric that contain
method resdiual chlorine,
add 1000 mg
ascorbic acid/L or
0.008% sodium
thiosulfate)
purge and trap keep in cool
capillary-column environment (< = 6
1,2- C); for samples that
0.03 gas
Dichloroetha - - contain volatile
mg/L chromatographic constituents but do
ne
/ mass not contain residual
spectrometric chlorine, add HCl to
 method pH <2.0 (4 drops, 1:1
HCl); for samples
that contain
resdiual chlorine,
add 1000 mg
ascorbic acid/L or
0.008% sodium
thiosulfate)
keep in cool
environment (< = 6
C); for samples that
contain volatile
purge and trap constituents but do
capillary-column not contain residual
1,2- gas chlorine, add HCl to
0.05
Dichloroethe - - chromatographic pH <2.0 (4 drops, 1:1
mg/L HCl); for samples
ne / mass
spectrometric that contain
method resdiual chlorine,
add 1000 mg
ascorbic acid/L or
0.008% sodium
thiosulfate)
keep in cool
environment (< = 6
C); for samples that
contain volatile
purge and trap constituents but do
capillary-column not contain residual
gas chlorine, add HCl to
Dichloromet 0.02
- - chromatographic pH <2.0 (4 drops, 1:1
hane mg/L HCl); for samples
/ mass
spectrometric that contain
method resdiual chlorine,
add 1000 mg
ascorbic acid/L or
0.008% sodium
thiosulfate)
keep in cool
liquid-liquid environment (< = 6
extraction gas C); for samples that
Di-(2-
0.008 chromatographic contain resdiual
ethylhexyl)pt - -
mg/L / mass chlorine, add 1000
halate mg ascorbic acid/L
spectrophotomet
ric method or 0.008% sodium
thiosulfate)
purge and trap keep in cool
capillary-column environment (< = 6
Ethylbenzen 0.3 gas C); for samples that
NDA 1.5 mg/L
e mg/L chromatographic contain volatile
/ mass constituents but do
spectrometric not contain residual
 method chlorine, add HCl to
pH <2.0 (4 drops, 1:1
HCl); for samples
that contain
resdiual chlorine,
add 1000 mg
ascorbic acid/L or
0.008% sodium
thiosulfate)
keep in cool
environment (< = 6
C); for samples that
contain volatile
purge and trap constituents but do
capillary-column not contain residual
gas chlorine, add HCl to
0.02
Styrene - - chromatographic pH <2.0 (4 drops, 1:1
mg/L HCl); for samples
/ mass
spectrometric that contain
method resdiual chlorine,
add 1000 mg
ascorbic acid/L or
0.008% sodium
thiosulfate)
keep in cool
environment (< = 6
C); for samples that
contain volatile
purge and trap constituents but do
capillary-column not contain residual
gas chlorine, add HCl to
Tetrachloroe 0.04
- - chromatographic pH <2.0 (4 drops, 1:1
thene mg/L HCl); for samples
/ mass
spectrometric that contain
method resdiual chlorine,
add 1000 mg
ascorbic acid/L or
0.008% sodium
thiosulfate)
keep in cool
environment (< = 6
C); for samples that
purge and trap contain volatile
capillary-column constituents but do
gas not contain residual
0.7
Toluene NDA 3.5 mg/L chromatographic chlorine, add HCl to
mg/L pH <2.0 (4 drops, 1:1
/ mass
spectrometric HCl); for samples
method that contain
resdiual chlorine,
add 1000 mg
ascorbic acid/L or
 0.008% sodium
thiosulfate)

keep in cool
environment (< = 6
C); for samples that
contain volatile
purge and trap constituents but do
capillary-column not contain residual
gas chlorine, add HCl to
Vinyl 0.0003
- - chromatographic pH <2.0 (4 drops, 1:1
Chloride mg/L HCl); for samples
/ mass
spectrometric that contain
method resdiual chlorine,
add 1000 mg
ascorbic acid/L or
0.008% sodium
thiosulfate)
keep in cool
environment (< = 6
C); for samples that
contain volatile
purge and trap constituents but do
capillary-column not contain residual
gas chlorine, add HCl to
Xylenes 0.5
NDA 5 mg/L chromatographic pH <2.0 (4 drops, 1:1
(total) mg/L HCl); for samples
/ mass
spectrometric that contain
method resdiual chlorine,
add 1000 mg
ascorbic acid/L or
0.008% sodium
thiosulfate)
keep in cool
liquid-liquid environment (< = 6
extraction gas C); for samples that
Aldrin and 0.00003 <0.02 chromatographic contain resdiual
NDA
Dieldrin mg/L μg/L / mass chlorine, add 1000
spectrophotomet mg ascorbic acid/L
ric method or 0.008% sodium
thiosulfate)
keep in cool
environment (< = 6
Atrazine and gas C); for samples that
its chloro-s- 0.1 chromatography contain resdiual
- -
trizine mg/L / mass chlorine, add 1000
metabolites spectrometry mg ascorbic acid/L
or 0.008% sodium
thiosulfate)
 add a sufficient
amount of
potassium
dihydrogen citrate
high- to yield a
performance concentration in the
0.007
carbofuran - - liquid sample of 9.2 to 9.5
mg/L g/L to prevent
chromatographic
method hydrolysis of oxamyl,
3-
hydroxycarbofuran,
carbaryl, and
methiocarb
keep in cool
liquid-liquid environment (< = 6
extraction gas C); for samples that
0.0002 <0.02 chromatographic contain resdiual
chlordane NDA
mg/L μg/L / mass chlorine, add 1000
spectrophotomet mg ascorbic acid/L
ric method or 0.008% sodium
thiosulfate)
keep in cool
environment (< = 6
C); for samples that
contain volatile
purge and trap constituents but do
capillary-column not contain residual
1,2-dibromo-
gas chlorine, add HCl to
2- 0.001
- - chromatographic pH <2.0 (4 drops, 1:1
chloropropa mg/L HCl); for samples
/ mass
ne that contain
spectrometric
method resdiual chlorine,
add 1000 mg
ascorbic acid/L or
0.008% sodium
thiosulfate)
keep in cool
liquid-liquid environment (< = 6
extraction gas C); for samples that
dichlorodiph
0.001 <0.04 chromatographic contain resdiual
enyltrichloro NDA
mg/L μg/L / mass chlorine, add 1000
ethane mg ascorbic acid/L
spectrophotomet
ric method or 0.008% sodium
thiosulfate)
keep in cool
liquid-liquid environment (< = 6
extraction gas C); for samples that
0.0006 <0.02 chromatographic contain resdiual
endrin NDA
mg/L μg/L / mass chlorine, add 1000
spectrophotomet mg ascorbic acid/L
ric method or 0.008% sodium
thiosulfate)
 keep in cool
environment (< = 6
C); for samples that
contain volatile
purge and trap constituents but do
ethylene capillary-column not contain residual
dibromide or gas chlorine, add HCl to
0.0004
1,2- - - chromatographic pH <2.0 (4 drops, 1:1
mg/L HCl); for samples
dibromoetha / mass
ne spectrometric that contain
method resdiual chlorine,
add 1000 mg
ascorbic acid/L or
0.008% sodium
thiosulfate)
liquid-liquid
extraction gas add 100 mg/L
sodium thiosulfate
chromatographic
glyphosate 1 mg/L - - for chlorinated
/ mass water and store at 4
spectrophotomet C away from light
ric method
keep in cool
liquid-liquid environment (< = 6
extraction gas C); for samples that
0.002 <0.02 chromatographic contain resdiual
lindane NDA
mg/L μg/L / mass chlorine, add 1000
spectrophotomet mg ascorbic acid/L
ric method or 0.008% sodium
thiosulfate)
liquid-solic
extraction and
capillary column
pendimethali 0.02
- - gas -
n mg/L
chromatography
/ mass
spectrometry
sensory
no
Taste - - evaluation keep cool at <= 6 C
taste
technique
sensory
Odor no odor - - evaluation keep cool at <= 6 C
technique
visual
comparison
Keep cool; analyze
Color 10 CU NDA 100 TCU method (for
same day
apparent color
only)
Nepehelometric
Turbidity 5 NTU - - keep cool at <=4 C
method
 Eriochrome
Cyanine R Nitric Acid
Digestion, Nitric
method,
0.2 Acid-Hydrochloric
Aluminum - - electrothermal
mg/L Acid Digestion;
atomic examine sample
absorption immediately
spectrometric
Cl-B
Argentometric no special
350
Chloride NDA 2 mg/L method, preservative is
mg/L necessary
Potentiometric
Method
Direct Air-
Acetylene Flame Nitric Acid
1.0 0.04 Method, Digestion, Nitric
Copper NDA
mg/L mg/L Extraction/Air- Acid-Hydrochloric
Acetylene Flame Acid Digestion
Method
Total 300 EDTA Titrimetric
- - Add HNO3 or H2SO4
hardness mg/L method
Methylene Blue
Method, Gas
hydrogen 0.05 Dialysis, preserve using zinc
- -
sulfide mg/L Automated acetate solution
Methylene Blue
Method
Direct Air-
Acetylene Flame Nitric Acid
1.0 Method, Digestion, Nitric
iron NDA 5 mg/L
mg/L Extraction/Air- Acid-Hydrochloric
Acetylene Flame Acid Digestion
Method
Electrometric
pH 6.5 - 8.5 NDA 6.0-9.0 none required
method
Direct Air-
Acetylene Flame
Nitric Acid
Method,
200 Digestion, Nitric
sodium - - Electrothermal
mg/L Acid-Hydrochloric
Atomic Acid Digestion
Absorption
Spectrometric
ion
chromatography
250 500 with chemical
sulfate NDA keep cool at 4 C
mg/L mg/L suppression of
eluent
conductivityt
 total dissolved
total dissolve 600
- - solids dried at 180 keep cool at 4 C
solids mg/L
C
Nitric Acid
Electrothermal Digestion, Nitric
Atomic Acid-Hydrochloric
5.0
zinc NDA 4 mg/L Absorption Acid Digestion;
mg/L acidify with
Spectrometric
Method concentrated nitric
acid to pH <2
keep in cool
environment (< = 6
C); for samples that
contain volatile
High- constituents but do
performance not contain residual
chlorine, add HCl to
0.0005 Liquid
acrylamide - - pH <2.0 (4 drops, 1:1
mg/L Chromatography HCl); for samples
with UV that contain
Detection resdiual chlorine,
add 1000 mg
ascorbic acid/L or
0.008% sodium
thiosulfate)
keep in cool
environment (< = 6
C); for samples that
contain volatile
purge and trap constituents but do
capillary-column not contain residual
gas chlorine, add HCl to
epichlorohyd 0.0004
- - chromatographic pH <2.0 (4 drops, 1:1
rin mg/L HCl); for samples
/ mass
spectrometric that contain
method resdiual chlorine,
add 1000 mg
ascorbic acid/L or
0.008% sodium
thiosulfate)
chlorine
0.4 analyse
dioxide - - Colorimeter
mg/L immediately
residual
chlorine analyze
1.5 DPD Colorimetric
residual (as - - immediately, keep
mg/L Method out of direct sunlight
free chlorine)
Ion
Chromatography
0.01 refrigerate
bromate - - wth Chemical
mg/L (unfiltered samples)
Suppression of
Eluent
 Conductivity
Single-Column
Ion
0.7 Chromatography refrigerate
chlorate - -
mg/L with Direct (unfiltered samples)
Conductivity
Detection
Single-Column
Ion filter on site (0.45 μ
0.7 Chromatography cellulose acetate
chlorite - -
mg/L with Direct membrane filter
Conductivity and freeze)
Detection
Gas
Chromatography
dibromoacet 0.07
- - / eelctron cool <= 6 C
onitrile mg/L
Capture
Detector
Gas
dichloroacet 0.05 Chromatography
- - refrigerate at 4 C
ate mg/L / Electron
Capture detector
Gas
dichloroacet 0.02 Chromatography
- - cool <= 6 C
onitrile mg/L / Electron
Capture detector
Micro Liquid-
Liquid Extraction
monochloro 0.02
- - Gas refrigerate at 4 C
acetate mg/L
Chromatographi
c Method
Micro Liquid-
Liquid Extraction
trichloroacet 0.2
- - Gas refrigerate at 4 C
ate mg/L
Chromatographi
c Method
refrigerate at 4 C,
keep in cool
Micro Liquid- environment (< = 6
2,4,6- Liquid Extraction C); for samples that
0.2
trichlorophe - - Gas contain resdiual
mg/L chlorine, add 1000
nol Chromatographi
c Method mg ascorbic acid/L
or 0.008% sodium
thiosulfate)
 keep in cool
environment (< = 6
C); for samples that
contain volatile
constituents but do
Closed-Loop not contain residual
Stripping, gas chlorine, add HCl to
0.1 Chromatographi pH <2.0 (4 drops, 1:1
bromoform - -
mg/L c/ Mass HCl); for samples
Spectrometric that contain
Analysis resdiual chlorine,
add 1000 mg
ascorbic acid/L or
0.008% sodium
thiosulfate); chill to 4
C
keep in cool
environment (< = 6
C); for samples that
contain volatile
purge and trap constituents but do
capillary-column not contain residual
gas chlorine, add HCl to
bromodichlo 0.06
- - chromatographic pH <2.0 (4 drops, 1:1
romethane mg/L HCl); for samples
/ mass
spectrometric that contain
method resdiual chlorine,
add 1000 mg
ascorbic acid/L or
0.008% sodium
thiosulfate)
keep in cool
environment (< = 6
C); for samples that
contain volatile
purge and trap constituents but do
capillary-column not contain residual
gas chlorine, add HCl to
0.3
chloroform - - chromatographic pH <2.0 (4 drops, 1:1
mg/L HCl); for samples
/ mass
spectrometric that contain
method resdiual chlorine,
add 1000 mg
ascorbic acid/L or
0.008% sodium
thiosulfate)
Closed-Loop keep in cool
Stripping, gas environment (< = 6
dibromochlo 0.1 Chromatographi C); for samples that
- -
romethane mg/L c/ Mass contain volatile
Spectrometric constituents but do
Analysis not contain residual
 chlorine, add HCl to
pH <2.0 (4 drops, 1:1
HCl); for samples
that contain
resdiual chlorine,
add 1000 mg
ascorbic acid/L or
0.008% sodium
thiosulfate)
The sum of the
ratio of the
concentration of
each to its
total THM 1 mg/L - - -
maximum
allowable level
should not
exceed 1.
Evaporation
concentrated HNO3
Gross alpha 0.5 Bq/L - - Method for Gross
or HCl to pH <2
Alpha-Beta
Co-precipitation
Method for Gross
concentrated HNO3
Gross beta 1.0 Bq/L - - Alpha
or HCl to pH <2
radioactivity in
drinking water
Bottles are to be
filled full (up to the
brim and no air
spaces); no
11.0 Liquid Scintillation
Radon - - acidification
Bq/L Method required; samples
are to be brought to
PNRI lab within the
day
Gamma
Gamma*
1 Bq/L - - Spectroscopic
(Ra-226)
Method
Gamma
Gamma* concentrated HNO3
0.1 Bq/L - - Spectroscopic
(Ra-228) or HCl to pH <2
Method
Gamma* (Sr- Gamma
90, I-131, Cs- 10 Bq/L - - Spectroscopic
134, Cs-137) Method
Liquid Scintillation
10000
Tritium*(H-3) - - Spectrometric no preservative
Bq/L
Method
2) What is the Chain of Custody protocol in laboratory testing?
- A laboratory will be using documentation chain of custody procedures
to maintain control and accountability of specimens. The date and
purpose shall be recorded on an appropriate Custody and Control
Form each time a specimen is handles or transferred and every
individual in the chain shall be identified. The following information
must at least be present:
 Information identifying the specimen
 Date and time of collection
 Name of testing laboratory
 Name and signature of all individuals who had custody of the
sample during the collection process
3) Why is it important to conduct calibration of instrument, measuring, and
testing equipment in the laboratory?
- Calibration of a laboratory instrument plays a big role when using that
instrument to measure and test different things. Instrument calibration
defines the accuracy and quality of the measurements. Poor
calibration can lead to highly inaccurate results, and uncertainty, so it
is important to regularly calibrate laboratory instruments/equipment in
order to prevent any measurement issues.